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Quantification of Reproductive Effort and Microscopic Observation on the Larval Development of Manila clam Ruditapes philippinarum (Adams and Reeve, 1850) (바지락 (Ruditapes philippinarum)의 번식량 측정 및 유생발달에 관한 연구)

  • Lee, Hee-Jung;Kang, Hyun-Sil;Park, Kyung-Il;Mondol, Mostafisur Rahman;Choi, Kwang-Sik
    • The Korean Journal of Malacology
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    • v.28 no.2
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    • pp.145-156
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    • 2012
  • Larval development of the Manila clam Ruditapes philippinarum reared in an indoor tank system was examined in this study using light microscope and scanning electron microscope. To induce spawning and subsequent larval development, clams were collected from the intertidal zone at Gim-nyeong harbor in Jeju Island in August 2011. After 2 days of rearing in the tank, all Manila clams spawned in the midnight. Non-feeding trochophore larvae appeared 7hrsafter fertilization and the first D-shape larvae could be observed at 19 hrs. Twenty one days after fertilization the pediveliger larvae crawling on the bottom of the tank with well-developed foot were observed. Histology indicated that all the clams used in this study were in the ripe stage prior to spawning and the gonad-somatic index (GSI), a ratio of the egg mass to the tissue weight, of the ripe female measured by ELISA was 28.6%. The GSI of female clam declined to 17.3% after the massive spawning in the tank, suggesting that Manila clam discharged 40% of the total eggs during the first spawning event. In conclusion, spawning and subsequent larval development of Manila clam was successfully carried out in this study using an indoor tank system, and the information obtained in the present study could be useful in future Manila clam hatchery development.

Haplotype Diversity and Gene Flow of the Diamondback Moth, Plutella xylostella(L.) (Lepidoptera: Yponomeutidae), in Korea (배추좀나방(나비목: 집나방과)의 haplotype 다양성과 유전자 이동률)

  • 김익수;배진식;최광호;진병래;이경로;손흥대
    • Korean journal of applied entomology
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    • v.39 no.1
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    • pp.43-52
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    • 2000
  • A portion of mitochondria1 COI gene (438 bp) was sequenced from the sampls of Plutella xylostella from four localities in Korea to investigate the population genetic structure and characteristics by measuring the magnitude of genetic diversity and the degree of gene flow among populations. Thirteen haplotypes ranging in nucleotide divergence 0.3% to 1.4%, were obtained from 21 individuals. The nucleotide divergence was similar to the other related studies, but haplotype diversity was substantially higher (mean h = 0.81). The genetic distance among geographically remote Cheju Island population and the two Kimhae populations, distant 1 lkm to each other, was not statistically significant (p<0.05). Instead, a substantial or high female gene flow was detected (Nm = 2-30). One Hawaiian haplotype of the diamondback moth obtained through GenBank search also was genetically similar to the ones obtained from this study. Collectively, the genetic population structure of the diamondback moth in Korea can be characterized into two aspects. First, the diamondback moths in Korea possesses overall moderate genetic divergence based on a high number of haplotypes. Second, a high haplotype diversity within each population due to the long distance dispersal with a substantial dispersal power and the resultant genetic similarity among geographic populations is characteristic.

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Reproduction of Extreme Waves Caused by Typhoon MAEMI with Wave Hindcasting Method, WAM (II) - Characteristics of extreme waves generated by Typhoon MAEMI in the south coast of Korea - (제3세대 파랑추산모형을 이용한 태풍 ‘매미’의 극한파랑 재현 (II) - 태풍 ‘매미’가 야기한 우리나라 남해안 일대의 극한파랑 특성 -)

  • Shin, Seung-Ho;Hong, Key-Yong
    • Journal of Navigation and Port Research
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    • v.28 no.8
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    • pp.745-751
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    • 2004
  • Following a preceding study of Shin et al.(2004), wave fields for a month of September of 2003 are simulated based on the modified WAM cycle 4 model that enables the precise wave hindcasting with fine spatial meshes, and characteristics of extreme waves at the south coast of Korea are analyzed The accuracy of applied wave model is verified by comparing computed wave parameters and corresponding ones measured at Ieodo ocean research station. The wave hindcasting of typhoon 'Maemi' with an hour time interval reveals the extreme wave characteristics at 4 primary locations of south coast of Korea as follows: 1) At the front sea of Chaguido in the south of Jeju-do, the maximum significant wave height, mean wave period and mean wave direction appear to be 7.41m, 13.65s and $6.4^{\circ}$ respectively at 16:00 KST of Sep. 12, 2003. 2) At the entrance of Masan Bay, 12.50m, 13.65s and $1.2^{\circ}$ at 21:00 KST of Sep. 12. 3) At the front sea of Suyoung Bay, 13.85m, 13.81s and $0.2^{\circ}$ at 22;00 KST of Sep. 12. 4) At the front sea of Ulsan port, l1.00m, 13.25s and $2.8^{\circ}$ at 23:00 KST of Sep. 12.

Survey on Personal Medicines in Cheju Island (제주도 민간요법에 관한 조사연구)

  • Lee, Kyung-Hee
    • Journal of East-West Nursing Research
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    • v.2 no.1
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    • pp.37-53
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    • 1997
  • The purpose is to inspect the personal medicines in Cheju island that are used traditionally and to get their characteristics. The subjectives are 39 Cheju people, men 10, women 29, who were horned, grown up in Cheju and agreed with this study. The ranges of age are 10 persons(m : 1, w: 9) over 40 less 50 years old, 14(m : 7, w : 7) over 50 less 60, 5(m : 1, w : 4) over 60 less 70, 6(m : 0, w : 6) over 70 less 80, and 4(m : 1, w : 3) over 80. The average age is 64.5 years old. The method to collect the data is 20 structured opening questionaires that are based on references. The duration to collect datas is 11days from 4th, Aprill 1997 to 14th, Aprill 1997. The workers who are trainned the interview methods went their villages and got answers after explaining the purpose and contents to them. Somtimes they used to record the answers. The analysis was identified the subjectives to four regions of Cheju, arranged answers with items, rearranged the same answers, counted with number and percentage. And classified the materials and characteristics. The results of this study are followed: The things that is used as personal medicines are the effects through experiences ans misteries that have hand down by word of mouth, even though they are not scientific. People used the materials arround their circumference. It is an accumulation of experiences. The ways used in eating, doing acupuncture or sting, exposing to smoke, wheedling, fixing after pounding. Almost materials are plants. Mugwort is effective in fever, gastritis, hemorrhoid, diarrhea, edematous hands or feet and dermatitis. Citron and Arrowroot in fever, gastritis. Seeds of Pumpkin in indigestive, hemorrhoid, edematous hands or feet. Gallic in fever, diarrhea, frostbite, dermatitis, and toothache. Motherwort in diarrhea, gastritis. Radish juice in indigestive, jaundice. Bean paste in burn, wound. Acupuncture in fever, gastritis, indigestive, back pain, edematous hands or feet. Sting as similar with it in fever, indigestive, edematous hands or feet. Cigarrette in hemorrhoid, wound, toothache. Cowstools in edematous hands or feet, wound. Sault is usded a lot in fever, gastritis, indigestive, hemorrhoid, uneffective voiding, edematous hands or feet, dermatitis, having a boil around the mouth, toothache, and eye disease. Japanese Parsley in fever, gastritis. Egg Apple in diarrhea, edematous hands or feet, frostbite. And Wild Chrysanthemum is effective in jaundice. In the conclusion, people used the things arround. A things is effective in several symptoms. If these are not effective, they would used the magic as god's anger. As locally, they used the grasses and fruits in the middle of Mt. Halla and seafood on the sea village.

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Synergistic Effects of Cinnamomum camphora Leaves Extract against Clinical Isolated Methicillin-resistant Staphylococcus aureus (임상에서 분리한 메치실린 내성 황색포도상구균에 대한 녹나무잎 추출물의 항균활성)

  • Cha, Jeong-Dan;Choi, Mi-Rae;Ko, Eun-Sil;Hwang, Seung-Mi;Kang, Jea-Ran;Oh, Joa-Sub;Park, Young-Jin;Jung, Young-Hwan;Jeon, Ah-Lim;Choi, Kyung-Min
    • Korean Journal of Plant Resources
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    • v.28 no.1
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    • pp.9-15
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    • 2015
  • Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important hospital and community pathogens. At the same time, because of the difficulty in developing chemical synthetic drugs and because of their side-effects, scientists are making more efforts to search for new drugs from plant resources to combat clinical multidrug-resistant microbial infections. Cinnamomum camphora (C. camphora) is a plant of family Lauraceae, and grown Jeju island in South Korea that are used as a drug to treat neurasthenia, epilepsy, cystitis, pyelonephritis, digitalis, cancer, and diabetes mellitus in folk remedies. In this study, antibacterial activites of 80% ethanol extract of C. camphora leaves (CCE) were investigated in combination with antibiotics against clinical isolates of MRSA. The results showed that CCE was determined with MIC and MBC values ranging from 156 to 313 and 313 to $625{\mu}g/ml$, oxacillin from 128 to 256 and 128 to $512{\mu}g/ml$, ampicillin from 4 to 64 and 8 to $128{\mu}g/ml$. The combination of CCE with oxacillin or/and ampicillin were synergistic effect against MRSA 1, 6, 7, 8, 10, 11, 12, 13, and 15/ MRSA 1, 2, 6, and 7.

Studies On the Major Components and Antioxidative Properties of Whole Fruit Powder and Juice Prepared from Premature Mandarin Orange (미숙 온주밀감을 이용한 통 밀감 분말과 착즙액의 성분함량 및 항산화성에 관한 연구)

  • Kang, Yeung-Joo;Yang, Min-Ho;Ko, Weon-Jun;Park, Seung-Rim;Lee, Bong-Gyu
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.783-788
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    • 2005
  • Major components and antioxidative properties of whole fruit powders and juices prepared from premature mandarin, Citrus unshu, were investigated. Dietary fiber, flavonoid, and organic acid contents greatly increased in samples with lower degree of fruit maturity (August) than higher ones (October), and in whole fruit powders than extracted juices, whereas free sugar contents, especially sucrose content, increased in fruits with higher degree of maturity than lower ones. Antioxidative properties were not affected by degree of fruit maturity, whereas total polyphenol content and electron-donating abilities were higher in extracted juices than whole fruit powders. Nitrite-scavenging abilities of all samples were similar except at pH 6.0.

Growth of Seedling and Germination Characteristics of Acanthopanax koreanum NAKAI (섬오갈피나무의 발아 및 유묘의 생육특성)

  • Ko, Han-Jong;Song, Chang-Khil;Cho, Nam-Ki
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.1
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    • pp.46-52
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    • 2003
  • This experiment was conducted to investigate the propagation for mass production of Acanthopanax koreanum NAKAI utilized for health food and medicine material. Germination rate of A. koreanum seeds were 64% when seeds were stored at 15 for 60 days and then were treated with 50 ppm of kinetin and dormancy were broken at 5 for 60 days. Rooting rates of green-wood cuttings treated with IBA 100 ppm, NAA 50 ppm, and IAA 100 ppm were 61.7, 56.7, and 60.0%, respectively. Rooting rates of greenwood cuttings treated with Rooton in scoria + horticulture media and volcanic ash were 76.7 and 66.7%, respectively. Survival rate of seedlings planted on Aug. 10 was highest (96%) under 75% shading net while shoot growth was best under 55% shading net. Survival rate of seedlings planted on May 2 was highest (91%) 55% shading net, and shoot growth was also best 55% under shading net.

Occurrence of Powdery Mildew Caused by Erysiphe abeliicola on Glossy Abelia in Korea (Erysiphe abeliicola에 의한 꽃댕강나무 흰가루병 발생)

  • Cho, Sung-Eun;Park, Ji-Hyun;Lee, Seung-Kyu;Lee, Sang-Hyun;Shin, Hyeon-Dong
    • Research in Plant Disease
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    • v.18 no.2
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    • pp.133-138
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    • 2012
  • In November 2009, a powdery mildew on glossy abelia (Abelia ${\times}$ grandiflora) was found in Seogwipo, Jeju Island, Korea. Further survey in the southern part of Korea, e.g., Jeju, Busan, and Tongyeong confirmed occurrence of the disease. White colonies were present on leaves, young stems, and flowers, detracting from their beauty in landscape plantings. Severely infected lesions were discolored to red-purplish. Based on the morphological characteristics and analysis of rDNA, the fungus associated with the symptoms was identified as Erysiphe abeliicola U. Braun & S. Takam. This work provides the morphological feature of its anamorph for the first time, which is characterized by having multi-lobed hyphal appressoria and short foot-cells of conidiophores. Morphological characteristics of mature chasmothecia were consistent with the previous Japanese record of this species. The sequence of internal transcribed spacer region of ribosomal DNA obtained from a Korean sample showed that this species places in the section Microsphaera of the genus Erysiphe in phylogenetic position, corresponding with the classical taxonomy. This is the first report of E. abeliicola and its host plant in Korea. The host plant A. ${\times}$ grandiflora is newly listed in the host range of E. abeliicola.

The Patterns of Conversion to Anti-Tuberculosis Drug Resistance in Mycobacterium tuberculosis (결핵균의 항결핵제 내성 전환 패턴)

  • Chong, Moo-Sang;Lee, Kyutaeg
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.2
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    • pp.125-132
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    • 2022
  • The prevalence of multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) are increasing. We analyzed the patterns of drug resistance and tracking period days of acquiring anti-mycobacterial resistance. From January 2010 to December 2019, drug susceptibility tests (DST) were performed by the absolute concentration method using the Löwenstein-Jensen solid medium and pyrazinamidase activity test (to assess pyrazinamide resistance) in samples from patients who were referred to the Green Cross Laboratories in Yongin. Among the cases that showed resistance to one or more anti-tuberculosis drugs, 55 patients (33.1%) were resistant to isoniazid (INH) at the time of initial referral, and the rates for the development of resistant anti-tuberculosis drugs were ethambutol (EMB) (26.6%), rifampicin (RFP) (21.9%), quinolones (QUI) (21.9%) and pyrazinamide (PZA) (10.9%), in that order. In the cases sensitive to all 10 anti-tuberculosis drugs initially, the development of resistance to INH was the most frequent, seen in 43 patients (7.2%). The average follow-up period was 435.6 days, and the resistance development was observed in the order of INH (7.2%), RFP (3.9%), SM (1.9%), QUI (0.7%), amikacin (AMK) (0.5%), and EMB (0.5%). The conversion of susceptible strains to resistant strains is an important warning sign for the patient, especially in cases of conversion to MDR or XDR. This information would be helpful for improving patient care during TB treatment.

The Effects of Depression on Fear of Recurrence and Health-related Quality of Life in Young Adults with Stroke (젊은 뇌졸중 환자의 우울감이 재발염려와 건강관련 삶의 질에 미치는 영향)

  • Yoon, Su-Sie;Han, Sang-Ho
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.4
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    • pp.371-380
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    • 2020
  • This study was conducted to investigate the effects of depression on fear of stroke recurrence and health-related quality of life in Young Adults with Stroke. The subjects of this study were 240 young stroke patients who visited the outpatient department of neurology at a tertiary hospital in C province and J university hospital from July 24, 2018, to November 22, 2018. The collected data were analyzed for statistical significance and hypothesis verification using SPSS 24.0 and SmartPLS 3.0. The results showed that guilt and mental depression in depression factors had an effect on fear of stroke recurrence, and mental depression had a statistically significant impact on health-related quality of life. Also, fear of recurrence has a significant impact on health-related quality of life. The results of this study suggest that overcoming fear of stroke recurrence by interventions that reduce guilt and mental depression through early assessment of their depression, thus it can significantly contribute to improving health-related quality of life in young adults with stroke.