• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.10a
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• pp.86-86
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• 2003

정향을 물, 메탄올, 에테르로 각각 추출하여 각 추출물별 항균 활성을 검토하였다. Paper-disc법에 의한 메탄올 추출물 항균효과는 E. coli, B. subtilis, S. aureus, S. thphimurium, P. aeruginosa에 강한 항균력를 보였다. 물 추출물의 경우 E. coli, S. aureus, S. thphimurium, P. aeruginosa에 대해선 강한 항균력을 보인 반면 B. subtilis에서는 항균력을 보이지 않았고 에테트 추출물의 경우에도 모든 균에 대하여 항균력을 보이지 않았다. E. coli, S. aureus, S.thphimurium, P. aeruginosa균에 대한 정향 물 추출물 및 메탄올 추출물의 농도가 증가할수록 생성된 clear zone의 크기도 증가하였다. (중략)

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.10a
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• pp.87-87
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• 2003

정향을 물, 메탄올, 에테르로 각각 추출하여 각 추출물별로 총페놀함량, 전자공여능(electron donating ability, EDA), 과산화물가와 에멀젼 시스템에서의 항산화효과를 비교, 검토하였다. Follin-Dennis법으로 구한 물, 메탄올 및 에테르 추출물의 총페놀 함량은 각각 2.34, 6.98, 5.83 mg/100g으로 메탄올 추출물에서 가장 큰 함량을 보였다. 정향의 물, 메탄올 및 에테르 추출물의 전자공여능은 각각 86%, 78%, 68%이었다. 리놀레인산 기질에서의 항산화효과는 메탄올 및 에테르 추출물에서 높게 나타난 반면, 에멀젼 시스템에서는 물 추출물이 가장 우수한 것으로 나타났다. (중략)

• Korean Journal of Organic Agriculture
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• v.22 no.2
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• pp.269-280
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• 2014

This study was performed to test the insecticidal and antimicrobial activity of plant extracts from clove, Sophora flavescens Aiton and neem. As the result of antimicrobial activity test, clove extract showed the strongest activity against Botrytis cinerea. In insecticidal activity test, the extract of Sophora flavescens Aiton was the highest against Nilaparvata lugens. The pyroligneous liquor (10%) and emulsified spreader (10%) were added to the extracts of clove and Sophora, respectively, to apply the environment-friendly rice field. In the field treated clove extract, disease damage occurred 49.1% and insect damage occurred 29.5% compared to control plot (100%). In the field treated Sophora extract, disease damage was 56.7% and insect damage was 21.0% compared to control plot (100%). In conclusion, plant extracts from Sophora and clove could control about 50% of disease and about 70% of insect damage that they could be used as environment-friendly resources to control disease and insect in rice farming.

• Journal of Conservation Science
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• v.10 no.1 s.13
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• pp.21-30
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• 2001

The antibacterial and insecticidal properties of ethanol extracts and volatile components extracted from Eugenia caryophyllata, Boswellia carterii, Agastache rugosa, Aristolochia contorta, and Aquilaria agallocha were evaluated. The ethanol extract and volatile component of E. caryophyllata showed strong antimicrobial effect against all strains (Mucor hiemalis, Aspergillus niger, Penicillium funiculosum, Trichoderma viride) and the volatile component of B. carterii showed antimicrobial effect against all strains except T. viride. The ethanol extract of E. caryophyllata and A. contorta showed $100\%\;and\;32\%$ mortality against Reticulitemes spertus kyushuensis Morimoto for 48 hours and 72 hours, respectively. In the case of volatile component, E. aryophyllata showed $100\%\;and\;20\%$ mortality against R. spertus and Lyctus linearis GOZE, respectively. The main constitute, eugenol $(92\%)$ among nine components from volatile component of E. aryophyllata were identified as antibacterial active substance.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.494-499
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• 2004

This study was investigated the antimicrobial activity of clove extracts according to extraction solvents. The extracts were tested for their antimicrobial activity against several food spoilage microorganisms including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa. The methanol extract showed stronger antimicrobial activities than water extract. However, petroleum ether extract did not show antimicrobial activity. The water extract of clove showed growth inhibition effect against Escherichia coli, Salmonella typhimurium and Staphylococcus aureus, whereas no effect against Bacillus subtilis. The methanol extract of clove extracts showed more sensitive antimicrobial activity in Gram (+) bacteria than in Gram (-) bacteria. The antimicrobial activities were increased with increasing concentration of the clove extract.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.10a
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• pp.83-83
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• 2003

대두유, 올리브유 및 채종유에 대한 정향추출물(clove extract)의 항산화 효과를 알아보고자 대두유, 올리브유 및 채종유에 정향추출물을 0.02%의 농도로 첨가한 후 180$\pm$2$^{\circ}C$에서 48시간동안 가열산화 시키면서 산가(acid value) 및 공액이중산가(conjugated diene value)를 측정하였다. 대두유, 올리브유, 채종유의 초기 산가는 0.13, 0.75, 0.11이었으며, 가열산화 후 0.52, 1.31, 0.51로 증가하였다. 또한 대두유, 을리브유, 채종유의 초기 공액이증산가는 0.57, 0.43, 0.35에서 가열산화 후 1.93, 1.04, 1.08으로 대두유보다 채종유나 올리브유의 산화안정성이 높았다. (중략)

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.4
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• pp.609-613
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• 2004

This study was investigated on antioxidant activities of water, methanol and ether extracts of clove. The extraction yields of water, methanol and ether extracts were 34.7, 21.4 and 17.0%, respectively. Total phenolic contents of water, methanol and ether extracts were 23.1, 55.7 and 34.9%, respectively. Eugenol contents were 6.1 and 8.1% in methanol and ether extracts, but it was not detected in water extract. Electron donating abilities of water, methanol and ether extract were 36.1, 30.9 and 29.7%, respectively. In linoleic aicd system, the ether extract showed more high antioxidant activity than water and methanol extracts. The antioxidant activity of ether extract was almost equal that of $\alpha$-tocopherol, but weaker than that of BHT In linoleic acid emulsion system, the water extract showed higher or stronger antioxidant activity than methanol and ether extracts. Antioxidant activities in linoleic acid emulsion substrates were in order of BHT ＞water extract＞methanol extract＞ether extract＞ $\alpha$-tocopherol.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.200.1-200
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• 2003

본 연구에서는 식품의 부패에 관여하는 미생물인 Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureu, Salmonella typhimurium Lactobacillus plantarum에 항균 활성을 나타내는 생약제의 효능을 살펴보았다. 금산 약초시장에서 구입한 생약재로부터 물 추출물과 70%에탄을 추출물을 제조하여 6종의 세균에 대해 항균활성을 탐색한 결과, 생약재 물 추출물의 경우에 가자 추출물은 E. coli, P. aeruginosa B. subtilis, S. aureus, S. typhimurium, L. plantarum의 6가지 균종에 대해서 모두 높은 항균활성을 나타내었으며, 복분자와 지유의 물추출물에서는 L. plantarum의 균종을 제외한 5가지 균종에 대해 높은 항균 활성을 나타내었다. 또한 소목 과 황련의 추출물에서는 E. coli를 제외한 5가지 균종에서 항균활성을 나타내었으며, 정향 추출물에서는 E. coli균주에 대해서는 약한 항균활성을 나타내었으나, B. subtilis, P. aeruginosa, S. aureus, S. typhimurium의 4종의 균주에 대해서는 우수한 항균활성을 나타내었다. 이 외에 감초 추출물은 P. aeruginosa, 석곡 추출물은 S. aureus, S. typhimurium, 계피 추출물은 S. typhimurium, 산수유 추출물은 B. subtilis, S. aureus, 파고지는 S. aureus균종에 대해서 항균활성을 나타내었다. 70% ethanol 추출물은 대부분의 생약재에서 높은 항균 활성을 나타내었다. 특히 가자, 감초, 강황, 복분자, 석곡, 소목, 오미자, 작약 ,정향, 지유, 파고지, 호장근, 황련 의 13가지 생약재 추출물에서 높은 항균활성을 나타내었다. 가자의 70% ethanol추출물의 경우는 물 추출물의 결과에서 같이 6가지 균종에 대해 모두 높은 항균활성을 나타냈으며, 복분자, 작약, 정향, 지유의 70% ethanol 추출물은 L. plantarum균을 제외한 5가지 균종에 대해 높은 항균 활성을 나타내었다. 소목의 70% ethanol 추출물의 경우에는 E. coli 균을 제외한 5가지 균종에서 높은 항균 활성을 보였으며, 이외에 생약재는 강황, 석곡, 황련, 호장근의 70% ethanol 추출물은 4가지의 균주에 대해서 항균 활성을 나타내었다. 또한 파고지, 오미자의 70% ethanol 추출물은 3가지 균주에 대해서 항균활성을 나타냈으며, 천궁, 육두구, 오수유, 갈근, 계피, 가시오가피의 70% ethanol 추출물은 2가지 균주에 대해 항균 활성을 나타내었다. 항균활성이 우수한 생약재를 농도별로 활성을 조사한 결과, 물 추출물과 10% Ethanol 추출물 모두 낮은 농도에서도 우수한 항균활성을 나타내었다.

• 한국문화재보존과학회:학술대회논문집
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• 2001.11a
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• pp.49-58
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• 2001

The antibacterial and insecticidal properties of ethanol extracts and volatile components extracted from Eugenia caryophyllata, Boswellia carerii, Agastache rugosa, Aristolochia contorta, and Aquilaria agallocha were evaluated. The ethanol extract and volatile component of E. caryophyllata showed strong antimicrobial effect against all strains (Mucor hiemalis, Aspergillus niger, Penicillium funiculosum, Trichodema viride) and the volatile component of B. carterii showed antimicrobial effect against all strains except T. viride. The ethanol extract of E. caryophyllata and A. contorta showed $100\%\;and\;32\%$ mortality against Reticulitermes spertus kyushuensis Morimoto for 48 hours and 72 hours, respectively. In the case of volatile component, R. aryophyllata showed $100\%\;and\;20\%$ mortality against R. spertus and Lyctus linearis GOZE, respectively. The main constitute, eugenol $(92\%)$ among nine components from volatile component of E. aryophyllata were identified as antibacterial active substance.

• Journal of the Korean Applied Science and Technology
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• v.37 no.3
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• pp.429-437
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• 2020

The purpose of this study is for checking anti-inflammatory effects of Syzygium aromaticum ethanol extract. For this, we investigated biological active evaluation about anti-inflammatory effects by Syzygium aromaticum ethanol extract. Syzygium aromaticum was extracted with 70% ethanol. This extract was tested for the cell viability on RAW 264.7 cell line by MTT assay, nitric oxide inhibitory activity and expression of inflammatory mediators. The extract showed low cytotoxicity as more than 90% cell viability in under 25 ㎍/ml concentration. On LPS-induced RAW 264.7 cell line, nitric oxide inhibition activity result showed that the extract reduced NO productions in a concentration-dependent manner. Expression of inflammatory cytokines as PGE₂, TNF-α and IL-1β decreased in a concentration-dependent manner and iNOS and COX-2 proteins expression were decreased significantly in western blot analysis. We confirmed that the Syzygium aromaticum ethanol extract has excellent anti-inflammatory effect. This results suggested that extract of Syzygium aromaticum may have value as the cosmetic materials.