• Title/Summary/Keyword: 정제과정

Search Result 872, Processing Time 0.022 seconds

Purification and Characterizatlon of a Cu, Zn-Superoxide Dismutase from Adult Paragonimus westermani (폐흡충 성충 Cu, Sn-Superoxide Dismutase의 정제 및 생화학적 특성)

  • 정영배;송철용
    • Parasites, Hosts and Diseases
    • /
    • v.29 no.3
    • /
    • pp.259-266
    • /
    • 1991
  • In cytosolic (raction of adult Paragonimus westermani, superoxide dismutase activity was identified (4.3 units/mg of specific activity) using a xanthine-xanthine oxidase system. The enzyme was purified 150 fold in its activity using the ammonium sulfate precipitation, DEAE-Trisacryl M anion-exchange chromatography and Sephadex G-100 molecular sieve chromatography. The enzyme exhibited the enhanced activity at pH 10.0. The enzyme activity totally disappeared in 1.0mM cyanide while it remained 77.8% even in 10 mM azide. These findings indicated that the ensyme was Cu, Zn-SOD type. Molecular mass of the enzyme was estimated to be 34 kDa by gel filtration and 17 kDa on reducing SDS-polyacrylamide gel electrophoresis which indicated a dimer protein.

  • PDF

Fast and Accurate Handling of Solid Collisions with Boundary Problem of Air Meshes (공기 메쉬의 경계 문제를 이용한 고체 충돌의 빠르고 정확한 처리)

  • Moon, Seong-Hyeok;Kim, Jong-Hyun
    • Proceedings of the Korean Society of Computer Information Conference
    • /
    • 2022.07a
    • /
    • pp.569-572
    • /
    • 2022
  • 본 논문에서는 공기 메쉬(Air meshes)를 이용하여 고체의 충돌을 효율적으로 처리할 수 있는 새로운 방법을 제시한다. 기존의 프리미티브 단위의 충돌 처리는 시뮬레이션의 안정성을 높이기 위해 시간 간격(Time-step), 3차 방정식과 같은 큰 계산 과정을 필요로 했으며, 장면 복잡도에 따라 DCD(Discrete collision detection)뿐만 아니라 CCD(Continuous collision detection)까지 고려해야 되는 상황이 빈번히 발생한다. 본 논문에서는 이전에 제안된 공기 메쉬 기법을 통해 충돌처리를 효율적으로 개선시킬 수 있는 방법에 대해서 제안한다. 원본 공기 메쉬 접근법은 시뮬레이션 메쉬가 아닌, 주변 공기를 메쉬화시키고 이들의 변형을 부피로 근사하여 충돌 여부 및 처리를 인지하고 예측했다. 공기 메쉬를 정제하는 과정에서 수치적인 수렴을 위해 정삼각형을 유지하려는 제약사항을 두었다. 하지만, 이러한 방법은 장면에 따라 노이즈한 결과를 나타내며, 헤어나 털 시뮬레이션과 같은 라인 형태인 시뮬레이션에서는 경계 문제가 더욱더 민감하게 나타났다. 본 논문에서는 공기 메쉬를 정제하는 과정에서 새로운 제약 조건을 추가하여 노이즈가 완화된 충돌처리 결과를 보여준다. 우리의 헤어뿐만 아니라 대부분의 장면에서 안정적인 결과를 보여준다.

  • PDF

Purification of Heat-Stable Enterotoxin of Enterotoxigenic Escherichia coli eKT-53 (장독성 대장균 eKT-53 균주의 내열성 장독소 정제)

  • Do, Dea-Hong;Kim, Kyo-Chang;Kim, Do-Young
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.21 no.1
    • /
    • pp.76-83
    • /
    • 1992
  • Enterotoxigenic E. coli is one of the major causative agents of the infantile diarrhea and traveler's diarrhea. The heat-stable enterotoxin (ST) is thought to be a virulence factor in the pathogenesis of the diarrhea and to be a maker for identification of the enterotoxigenic E. coli from non pathogenic E. coli. ST producing E. coli KM-7 strain was isolated from the swine and molecular cloning of ST gene of KM-7 strain. Transformant eKT-53 $(ST^+,\;LT^-)$ was selected by infant mouse assay (IMA). The culture supernatant of eKT-53 strain was performed purification by multipled steps. The culture supernatant (crude ST) was purified by sequentially applying batch adsorption chromatography on Amberlite XAD-2 resin, ion exchange chromatography on DEAE-Sephacel anion exchanger, gel filtration chromatography on Bio-Gel P-6 and preparative polyacrylamide slab gel electrophoresis. About 113-fold purification was achieved with a yield of about 11% of crude ST and the minimum effective dose(MED) of this purified ST was about 2.8ng in IMA. Homogeneity of purified ST was demonstrated by showing a single band in analytical SDS polyacrylamide disc gel electrophoresis.

  • PDF

Purification of TGF-$\beta$ 1 from Human Platelets by an Improved Method (개량된 방법에 의한 사람혈소판으로부터 TGF-$\beta$ 1의 분리)

  • 신충건;김상국;문병조;김평현;전계택;남상욱;김장환;이종원
    • KSBB Journal
    • /
    • v.14 no.1
    • /
    • pp.9-16
    • /
    • 1999
  • Transforming growth factor $\beta$1(TGF-$\beta$1) has potentials to be used as a new therapeutic agent. However, studies with TGF-$\beta$ were hindered by its high cost. In this study, we developed an improved method to purify TGF-$\beta$1 from human platelets, for which four purification steps were used: platelet extraction, gel filtration, cation exchange chromatography, and reverse phase high performance liquid chromatography. After a final step of purification, a pure protein with a molecular weight of 25,000 corresponding to the commercially available TGF-$\beta$1 was obtained, which were confirmed by silver staining and Western blotting after SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It was confirmed by the inhibitory effects of TGF-$\beta$1 on a mink lung epithelial cell line that the purified TGF-$\beta$1 had its biological activity, whose activity is slightly higher than that of the commercially available TGF-$\beta$1. About 3.7$\mug of the purified TGF-$\beta$1 was obtained from 10 units of concentrated human platelets, the final yield of which is about 21%.

  • PDF

Quality Characteristics of Kimchi with Added Purified Licorice(Glycyrrhiza uralensis) Extract (감초정제물 첨가 김치의 품질특성)

  • Lee, Su-Hyun;Ko, Young-Tae
    • Korean journal of food and cookery science
    • /
    • v.22 no.5 s.95
    • /
    • pp.609-616
    • /
    • 2006
  • The effects of purified licorice (Glycyrrhiza uralensis) extract (PLE) as a sugar substitute on kimchi quality were evaluated by investigating acid formation, growth of lactic acid bacteria, sensory properties, and volatile odor components of PLE-added kimchi. The pH of kimchi with higher amounts of added PLE increased slightly with two or three days ripening. The acidity of unripened kimchi or kimchi ripened for one day significantly increased with addition of PLE, while that of kimchi ripened for two or three days decreased significantly (p<0.05). Addition of PLE had no significant effect on the lactic acid bacteria count of kimchi compared to that of sugar. Overall acceptability and taste of 0.005 or 0.01% PLE-added kimchi ripened for two to three days were higher than those of other samples, whereas addition of more than 0.01% PLE to kimchi unripened or ripened for one day resulted in lower overall acceptability and taste than the reference sample. Diallyl sulfide and methyl trisulfide were newly produced by ripening of kimchi, and the amounts of some volatile odor components in kimchi were also changed during ripening.

Purification and Characterization of Invertase in Astringent Persimmon during Sun Drying (건시제조 중 Invertase의 정제 및 그 특성)

  • Lee, Byung-Ou;Moon, Kwang-Deog;Shon, Tae-Hwa
    • Journal of the Korean Society of Food Culture
    • /
    • v.5 no.2
    • /
    • pp.269-274
    • /
    • 1990
  • This study was conducted to determine invertase activity in persimmon during the drying process and characterize the purified enzyme. As drying proceeded, invertase activity increased until 10 days and decreased gradually afterwards. Invertase in persimmon fruit was extracted with 250 mM potassium phosphate sulfate buffer at pH 7.4. The enzyme was purified by means of ammonium sulfate fractionation, column chromatography on DEAE-cellulose and gel filtration on Sephadex G-200 column. The optimal temperature of enzyme was $40^{\circ}C$ and optimal pH was 5.0 and 6.0 for sucrose and raffinose, respectively. The enzyme was stable up to $50^{\circ}C$ and pH 3-6. The Km value of the enzyme, with sucrose as a substrate, was 2.5mM. Electrophoretic pattern of purified enzyme solution showed a single band.

  • PDF

The analytical study on synthesis and purification of high purity ionic liquid, 1-ethyl-3-methylimidazolium tetrafluoroborate (이온성 액체 1-ethyl-3-methylimidazolium tetrafluoroborate (EMI-BF4)의 합성과 정제에 관한 분석 연구)

  • Yang, Kyung-Chul;Lee, Young-Hwan
    • Analytical Science and Technology
    • /
    • v.24 no.6
    • /
    • pp.477-483
    • /
    • 2011
  • This work is on the synthesis of EMI-$BF_4$ (1-ethyl-3-methylimidazolium tetrafluoroborate) and purification of spectroscopic grade using aluminium oxide method, activated charcoal method, and liquid/liquid fractional extraction method in order to make supercapacitor finally. But the aluminum oxide method and the activated charcoal method were not suitable for obtaining high-purity ionic liquids. The liquid/liquid fractional distillation method turned out that as the concentration of solvent ($H_2O$) was increased, the higher purity of EMI-$BF_4$ was obtained and the electrical capacity of this compound was increased to higher value. When the solvent was changed to from methylene chloride to 1,2-dichloroethane, the higher purity of EMI-$BF_4$ was obtained.

A Study on the Alkaline Protease Produced from Bacillus subtilis (Bacillus subtilis가 생산하는 Alkaline Protease에 관한 연구)

  • Chang, Shin-Jae;Kim, Yoon-Sook;Sung, Ha-Chin;Choi, Yong-Jin;Yang, Han-Chul
    • Applied Biological Chemistry
    • /
    • v.31 no.4
    • /
    • pp.356-360
    • /
    • 1988
  • The alkaline protease producing bacteria isolated from soil and identified as Bacillus subtilis. The optimum medium for alkaline protease production from the microorganism was as follows; soluble starch, 1.5% ; proteose peptone, 0.5% ; $K_2HPO_4$, 0.1% ; $MgSO_4{\cdot}7H_2O$, 0.02% and sodium carbonate, 1.0%. The optimum temperature for alkaline protease production was $35^{\circ}C$, and the initial pH of medium was pH 10.5. The alkaline protease activity was about 2,300 U per ml of culture broth by Casein-Folin Method. A 9.2 fold purification of alkaline protease was obtained from culture broth. The recovery was 14% and purified enzyme was identified as single band, and its molecular weight was about 19,000. The optimum temperature for enzyme reaction was $70^{\circ}C$, and optimum pH was 12. The activity of purified enzyme was inhibited by metal ion ($Fe^{++}$), and Phenylmethylsulfonyl Fluoride, a serine protease inhibitor.

  • PDF

Studies on Antitumor Components of Cultured Basidiomycetes - Purification and Chemical Analysis of Antineoplastic Constituents of Cultured Mycelia of Laccaria laccata - (애기졸각버섯 배양(培養) 균사(菌絲)의 항암(抗癌) 성분(成分)의 정제(精製) 및 화학(化學) 분석(分析))

  • Kim, Yoo-Jin;Lee, Chong-Ock;Shim, Mi-Ja;Kim, Sung-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • The Korean Journal of Mycology
    • /
    • v.12 no.1
    • /
    • pp.35-43
    • /
    • 1984
  • To produce and characterize antineoplastic constituents in the submerged cultured­mycelia of Laccaria laccata, the mycelia were extracted with distilled water. Purification of the extract was carried out by acetone precipitation, by ion exchange chromatography using DEAE­Sephadex A-50, CM-Sephadex C-25 resins, and by gel filtration chromatography on Sephadex G-200. Each fraction obtained during the purification was examined for antineoplastic activity against sarcoma 180 in ICR mice. As the purification proceeded, the antineoplastic activity was markedly increased. The highly purified Fraction E showed 75% tumor inhibition ratio at a dose of 10mg/kg/day and contained 81% polysaccharide and 4% protein. The antitumor component of Fraction E stimulated an accumulation of peritoneal exudate cells including peritoneal macrophages, and is named laccaran.

  • PDF

Characterization and Pharmacological Effect of Mung Bean Trypsin Inhibitor (녹두(Vigna radiata L.) Trypsin Inhibitor의 정제 및 약물학적 특성)

  • 문성은;신영희
    • Journal of Life Science
    • /
    • v.12 no.5
    • /
    • pp.528-534
    • /
    • 2002
  • A kypsin inhibitor was isolated and purified from Mung bean (Vigna radiata L. wilczek) which has been used as a galenic and traditional food. In addition, we evaluated the pharmacological effect of the mung bean trypsin inhibitor (MBTI) using septic shock induced guinea pig model. Purification was carried out by Sephadex G-50 gel filtration, DEAE-cellulose ion exchange chromatography, and trypsin affinity column. The molecular weight of MBTI was estimated to be about 8,000 Da by 20% SDS-PACE under reducing condition. The chemically determined partial amino acid sequences of the purified MBTI perfectly coincide with those of previously reported MBTI which is BBI type trypsin inhibito. (Bowman-birk inhibitor type). These results suggest that the purified MBTI is authentic. Hypotension shock was prevented by the pretreatment of the MBTI (10 mg/kg of the body weight) on the septic shock guinea pig model caused by psedomonal elastase.