• Title/Summary/Keyword: 정자기장

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THE EFFECT OF STATIC MAGNETIC FIELDS ON MOLECULAR AND CELLULAR ACTIVITIES (정자기장이 효소와 세포 활성에 미치는 영향)

  • Park, Jae-Gu;Hwang, Hyeon-Shik
    • The korean journal of orthodontics
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    • v.27 no.6 s.65
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    • pp.929-941
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    • 1997
  • Optimal force for orthodontic treatment is the force that produces a rapid rate of tooth movement without discomfort to the Patient or ensuing tissue damage. Recently considerable interest has been generated in the application of magnets as a way to obtain an optimal force. The purpose of the present study was to investigate the effect of static magnetic fields of Sm-Co magnets on molecular and cellular activities. The distance of erythrocyte sedimentation was measured directly, and the activities and the syntheses of $Fe^{2+}$-related enzymes (catalase and NO synthase) and non $Fe^{2+}$-related enzyme (lactic dehydrogenase) were assayed by the spectrophotometer. The growth and the proliferation of osteoblast-like cells $MC_3T_3-E_1$ were determined by the crystal violet staining and the ${^3}H$-thymidine incorporation. The erythrocytes were exposed to the pole face flux density of 1,400 G (gauss), and the enzymes and osteoblast-like cells $MC_{3}T_3-E_1$ were exposed to the flux density of 7,000 G. The results obtained were as follows: 1. The distance of sedimentation of erythrocyte was not affected by the static magnetic fields. 2. The activities of catalase and lactic dehydrogenase were not affected by the static magnetic fields. 3. The intracellular syntheses of NO synthase and lactic dehydrogenase were not affected by the static magnetic fields. 4. The growth and the proliferation of cultured osteoblast-like cells $MC_{3}T_3-E_1$ were not affected by the static magnetic fields. These results suggested that the molecular and cellular activities were not significantly influenced by the static magnetic fields.

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The Effects of Static Magnetic Field and Pulsed Electromagnetic Field on Alkaline Phosphatase and DNA synthetic Activity of ME3T3-E1 Cells (정자기장과 맥동전자기장이 MC3T3-E1 세포의 ALP 및 DNA 활성도에 미치는 영향)

  • Son, Jeong-Hee;Bae, Seong-Min;Sung, Jae-Hyun
    • The korean journal of orthodontics
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    • v.27 no.4 s.63
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    • pp.623-632
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    • 1997
  • The purpose of this study was to evaluate the effects of magnetic field on cellular activity of MC3T3-E1 cells. The cellular activity was monitored by alkaline phosphatase and DNA synthetic activity in control, static magnetic field and pulsed electromagnetic field groups. A static magnetic field was applied to the cell by placing one, two, three, foue, and five samarium-cobalt magnets above and below each cell plate for 24hours per day. A pulsed electromagnetic field with a frequency of 100 herz was applied for 10 hours per day. After 10 days of magnetic field exposure, there were increase of alkaline phosphatase activity in static magnetic field groups consisted of one, two and three magnetic groups. Alkaline phosphatase activities were not significantly increased in four and five magnetic groups. Application of pulsed electromagnetic field did not result in significant increase in alkaline phosphatase activity compared to control. DNA synthetic activity in both static and pulsed electromagnetic field group were not significantly different from that in control group. The result of this study suggest that magnetic field could have effect on the metabolism of bone cells related to the cellular metabolic process.

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Guidelines of IRPA/ICNIRP for Non-ionizing Radiation (비이온화방사선에 대한 IRPA/ICNIRP의 제반지침)

  • Lee, Soo-Yong
    • Journal of Radiation Protection and Research
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    • v.20 no.3
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    • pp.143-154
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    • 1995
  • In recent years, the development of new technologies using static magnetic fields has increased the possibility of human exposure to these fields and raised some concern as to their possible health effects. In several countries, governmental or other competent authorities have issued exposure limits that are mainly intended for specific uses, i.e., magnetic resonance imaging (MRI) and particle accelerators for high-energy Physics. Since applications of magnetic fields in industry and medicine are likely to grow in the future, thus increasing the possibility of occupational and general public exposure, and since the number of people with ferromagnetic implants and implanted electronic devices that can be affected by the fields is growing, there is a need for international guidelines. In the present papers, guidelines on limits of exposure to static magnetic fields are selected and discussed in order to review the guidelines of the International Non-ionizing Radiation Committee of the International Radiation Protection Association (IRPA/INIRC) for non-ionizing radiation(NIR)

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Prediction for Underwater Static Magnetic Field Signature Generated by Hull and Internal Structure for Ferromagnetic Ship (강자성 함정 선체 및 내부 장비에 의한 수중 정자기장 신호 예측)

  • Yang, Chang-Seob;Chung, Hyun-Ju;Ju, Hye-Sun;Jeon, Jae-Jin
    • Journal of the Korean Magnetics Society
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    • v.21 no.5
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    • pp.167-173
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    • 2011
  • Underwater static magnetic field signature for the naval ship has been widely used as the detonating source of the influence mine system because it is possible to make an accurate target detection in the near field although the magnetic field falls off relatively fast with distance in comparison with the underwater radiated noise signal. In this paper, we describe the prediction results about the underwater static magnetic field by the ferromagnetic hull, the internal structures and the main on-board equipment for the target vessel using the commercial FEM software. Also we analyze the degaussing effectiveness for the target vessel through the degaussing coils arrangement.

The Effect of A Static Magnetic Field on The Bone Nodule Formation of MC3T3-E1 Cells (정자기장이 MC3T3-E1 세포의 골결절형성에 미치는 영향)

  • Kim, Kwang-Duk;Kwon, Oh-Won;Ryu, Hyun-Mo
    • The korean journal of orthodontics
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    • v.28 no.1 s.66
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    • pp.155-163
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    • 1998
  • To evaluate the effect of a static magnetic field on the bone producing potential of MC3T3-E1 cells, the alkaline phosphatase activity was measured after the cells having been cultured under 76.4mT static magnetic field using a $SmCo_5$ magnets for 5days, 7days, 11days, 15days and 21days for each cell culture group. Also, the amount of bone nodule stained with Alizarin red S was observed. The results were as follows . The alkaline phosphatase activity of the 7, 11, and 15 days group among the experimental groups was decreased as compared with the control groups, and the decrease of alkaline phosphatase activity in the 11 days group was the most evident among them. . Any stained bone nodules of both groups had not been observed until the 11th day. The stained bone nodules in the control groups were found on the 15th day, but not in the experimental groups. The stained bone nodules were observed in both groups on the 21st day, but the control groups have more bone nodules than the experimental groups.

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The effect of a static magnetic field of Nd-Fe-B magnet on alkaline phosphatase activity of MC3T3-E1 cells (Nd-Fe-B 자석의 정자기장이 MC3T3-E1 세포의 alkaline phosphatase 활성도에 미치는 영향)

  • Kim, Sook-Hee;Kwon, Oh-Won;Ryoo, Hyun-Mo
    • The korean journal of orthodontics
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    • v.30 no.2 s.79
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    • pp.205-214
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    • 2000
  • Recently, the magnetic force has been considered as a method for a more efficient tooth movement. The purpose of this study was to evaluate the effects of different static magnetic fields of Nd-Fe-B magnet on MC3T3-E1 cells by measuring the alkaline phosphatase activity and observing the amount of stained alkaline phosphatase. For measuring of alkaline phosphatase activity, MC3T3-E1 cells were seeded in first and third row of 12 well culture plates. And Nd-Fe-B magnets were positioned under the first column of first and third row to apply different static magnetic fields(first column:100mT ; second column:4.6mT ; third column:0.5mT ; forth column:0.0mT) to the cells for 7, 13, 19, and 25 days. For staining of alkaline phosphatase, MC3T3-E1 cells were seeded in 100mm culture plates. And Nd-Fe-B magnets were positioned under the corner of plates to apply different static magnetic fields(magnet side:100mT : the opposite side:0.5mT) to the cells for 7, 13, 19, and 25 days. The results were as follows : 1. ALP activity was increased until day 19 in biochemical determination as well as in histochemical staining, 2. The application of higher magnetic field(100mT) suppressed ALP activity at day 13, 19, 25. On the contrary, the application of the lower magnetic field(4.6mT, 0.5mT) significantly enhanced the ALP activity. 3. Consistent with enzyme assay, histochemical staining of ALP also demonstrated that higher magnetic field(100mT) suppressed ALP activity, lower one(0.5mT) enhanced.

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