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The Inhibitory Potency of Hesperidin on Protein Kinase C Activity Using a Biochip (바이오칩을 이용한 Protein Kinase C의 활성에 대한 헤스페리딘의 저해 효과)

  • Kang, Jung Ae;Rho, Jong Kook;Choi, Mi Hee;Jung, Young Jin;Park, Sang Hyun
    • Journal of Radiation Industry
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    • v.5 no.1
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    • pp.15-20
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    • 2011
  • Protein kinases are the most important drug targets for the treatment of numerous diseases. The involvement of protein kinase C (PKC) in many biological processes such as development, memory, cell differentiation, and proliferation has been demonstrated. PKC is recognized as an important player in carcinogenesis. Thus, a variety of PKC inhibitors have been investigated. Among them, flavonoids have been demonstrated to affect the activity of many mammalian in vitro enzyme systems. The recent investigation was performed to evaluate the inhibitory effects of hesperidin, which is a flavonoid, on the proliferation and carcinogenesis of many cancers. In this study, an efficient kinase assay based on a biochip using radio-phosphorylation was established and performed for an examination of the inhibitory effects of hesperidin on PKC activity at different concentrations of 50, 200, 500 nM. It was found that hesperidin shows inhibitory potency on PKC, and that the biochip can be used to rapidly screen kinase inhibitors resulting in the therapeutic agents.

Quality Characteristics of Soybean Pasted (Doenjang) Manufactured with 2 Soybean Mutant Lines Derived from cv. Baekwon (백운콩 돌연변이 후대로 제조한 된장의 품질 특성)

  • Lee, Kyung Jun;Kang, Si-Yong;Choi, Hong-Il;Kim, Jin-Baek
    • Journal of Radiation Industry
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    • v.10 no.1
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    • pp.13-20
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    • 2016
  • In order to identification of the possibility of manufacturing soybean paste (doenjang) with soybean mutant lines induced from gamma-ray mutagenesis, this study was performed to investigate the quality characteristics of doenjang using two soybean mutant lines, Baekwon-1 (BW-1) and Baekwon-2 (BW-2) and their original cultivar (cv. Baekwon, BW) for 8 weeks. The BW and two mutant lines (BW-1 and BW-2) were showed higher content of amino type nitrogen than control (cv. Taegwang). The pH decreased and the titratable acidity increased all the samples during aging period. The lightness, redness and yellowness of doenjang were the lowest in BW. Total free sugar content of doenjang was the highest in control (10.43%) after 4 weeks and composed mainly fructose and glucose. The order of the free amino acid content was Glutamic acid>Leucine>Lysine>Phenylalanine>Aspartic acid in control, Glutamic acid>Leucine>Arginine>Lysine>Phenylalanine in BW, Glutamic acid>Lysine>Phenylalanine>Aspartic acid>Valine in BW-1 and Glutamic acid>Arginine>Lysine>Phenylalanine>Aspartic acid in BW-2, respectively. Our results showed that it is possible to increase the quality of doenjang using soybean mutant lines in manufacturing soybean paste.

Effects of Gamma Ray Irradiation on the Struvite Crystallization of Livestock Wastewater (축산폐수를 이용한 스트러바이트 합성에 감마선 조사가 미치는 영향)

  • Yoo, Byeong-Hak;Jo, Seong-Hui;Lee, Myun-Joo;Kim, Tak-Hyun
    • Journal of Radiation Industry
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    • v.4 no.4
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    • pp.313-317
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    • 2010
  • The struvite crystallization was applied to remove $NH_4{^+}$ in livestock wastewater. The ammonium ions can be very toxic to the aquatic creatures. In this experiments, the livestock wastewater from Gongju livestock wastewater treatment plant was used. The behaviors of various parameters such as pH, mole ratio of $Mg^{2+}$ : $NH_4{^+}$ : $PO{_4}^{3-}$ and reaction temperature for struvite crystallization of livestock wastewater and the effect of gamma ray irradiation were evaluated. As results, for the pH variation, the $NH_4{^+}$ removal efficiency showed the highest, 88%, at pH 9~9.25. The removal efficiency of $NH_4{^+}$, $Mg^{2+}$ and $PO{_4}^{3-}$ was showed highest when same molar ratio of $Mg^{2+}$ and $PO{_4}^{3-}$ were applied. The $NH_4{^+}$ removal efficiency showed 82% at $7^{\circ}C$, and 90% at $30^{\circ}C$ with temperature. When the wastewater was irradiated with 20 kGy of gamma ray, $NH_4{^+}$ was removed as much as 83%.

Analysis on the temporal and spatial variation of groundwater recharge rate in Jeongup area (정읍지역 지하수 함양량의 시공간적 변동해석)

  • Chung, Il-Moon;Na, Han-Na;Kim, Nam-Won;Lee, Duk-Su
    • Proceedings of the Korea Water Resources Association Conference
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    • 2010.05a
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    • pp.2016-2020
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    • 2010
  • 기존에 국내외에서 적용되는 지하수 함양량 추정방법인 기저유출 분리법, 연단위 물수지 분석법, 지하수위변동법 등은 집중형 개념을 기반으로 하거나 국지적인 규모로 다뤄지기 때문에 함양량의 시공간적 변동성을 나타내기에는 한계가 있다. 따라서 본 연구에서는 시공간적으로 변하는 지하수 함양량을 정량적으로 추정할 수 있는 기법을 이용, 실제 유역에 적용하였다. 이를 위해 강우-유출 모형은 수문성분 해석 이론이 잘 정립된 SWAT모형과 SWAT모형의 지하수 부분을 MODFLOW모형으로 대체한 SWAT-MODFLOW모형을 선택하였고, 분석 대상유역인 정읍지역을 대상으로 각 소유역 및 수문학적 반응단위(Hydrologic Response Unit: HRU)별로 토지이용과 토양통 특성을 반영하여 지하수 함양량의 시공간적인 변화를 산정하였다. 2001년부터 2008년까지의 소유역별 일단위 지하수 함양량을 산정하였으며, 함양량의 시 공간적 변동성을 분석한 결과 월평균 함양량의 경우 대략 280mm 범위 내에서 유역의 토지이용 및 토양특성, 경사 등에 따라 매우 비 균질하게 분포하는 것을 확인할 수 있었다. 이렇게 산정한 함양량은 지역지하수 관리계획에 유연하고 합리적으로 적용될 수 있을 것으로 판단된다.

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A Study of Interface Layer on CdZnTe Radiation Sensor for Potable Isotope Identifier (이동형 핵종 분석 장치용 CZT 반도체 검출기의 완충전극에 대한 연구)

  • Cho, Yun Ho;Park, Se-Hwan;Kim, Yong Kyun;Ha, Jang Ho
    • Journal of Radiation Industry
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    • v.5 no.1
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    • pp.95-99
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    • 2011
  • The electrical and mechanical properties of electrode for radiation detection are very important. In general, Au electrode and CZT crystal are combined to form ohmic contacts, and the best energy resolution is shown at the Au electrode. The metal contacts are fabricated by electroless deposition method, sputtering deposition method and thermal evaporation method. The electrode fabrication is easy with use of the thermal evaporation method, while an adhesive strength is weak. Thus interface materials such as Ag, Al and Ni were investigated to overcome defects generated by the this method. The thickness of the interface material between the Au electrode and the CZT crystal was 100 Angstroms, the Au electrode with thickness of 400 Angstroms was deposited. The Al+Au electrode is shown that the results of current-voltage and radiation response are similar to results of Au electrode.

Electrochemical Study on PVDF-HFP/Silylated Al2O3-coated PE Separators using the Electron Beam Irradiation for Lithium Secondary Battery (전자선을 이용한 PVDF-HFP/Silylated Al2O3가 코팅된 리튬 이차 전지용 폴리에틸렌 분리막의 전기화학적 특성 연구)

  • Sohn, Joon-Yong;Shin, Junhwa;Nho, Young-Chang
    • Journal of Radiation Industry
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    • v.4 no.4
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    • pp.359-364
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    • 2010
  • PVDF-HFP (binder)/silylated alumina (inorganic particle)-coated PE (polyethylene) separators were with various compositions of binder and inorganic particle were prepared by a dip-coating process with humidity control (R.H. 25% and 50%) using electron beam irradiation. The morphology of the coated PVDF-$HFP/Al_2O_3$ layer with various compositions of PVDF-HFP and $Al_2O_3$, and humidity condition was found to be an important factor in determining ionic conductivity of the prepared separators. The PVDF-$HFP/Al_2O_3$ (5/5)-coated PE separator prepared at R.H. 50% followed by electron beam irradiation at 200 kGy was applied for lithium-ion polymer battery and the cell test results showed improved high-rate discharge performance and better cyclic stability compared to the cells with the bare PE and the PVDF-HFP-coated PE separators.