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감마선 조사에 의한 감귤의 잔류농약 저감화 연구 (Study on Diminution of Pesticide Residues in Citrus Fruits by γ-Irradiation)

  • 양희선;박용대;진창현;최대성;정일윤
    • 방사선산업학회지
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    • 제3권1호
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    • pp.19-24
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    • 2009
  • The citrus fruits were irradiated at different doses (25~200 kGy) to remove pesticides such as diuron, dichlorbenil, trifluralin, dimetoate, chlorothalonil, fenitrothion, bromacil, phenthoate and norflurazon, and the rates of degradation were detected by using gas chromatography electron capture detector (GC-ECD). The limit of detection (LOD) was in the range of 0.007~0.105 ppm. The limit of quantification (LOQ) was in the range of 0.024~0.352 ppm, and the recoveries of 9 pesticides were in the range of 71.5~106.5%. The results demonstrated that the ${\gamma}$-irradiation was effective method to remove pesticides such as diuron, dichlorbenil, trifluralin, dimetoate, chlorothalonil, fenitrothion, and norflurazon. However, the bromacil and phenthoate showed lower removal rates in the rate of 40.5 and 54.8%, respectively, at 200 kGy irradiation.

감마선을 이용한 Escherichia coli K12의 살균에서 persulfate의 효과 (Effect of Persulfate on Disinfection of Escherichia coli K12 by Gamma Radiation)

  • 이오미;김태훈;유승호;정인하;이면주
    • 방사선산업학회지
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    • 제5권1호
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    • pp.63-67
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    • 2011
  • A comparative experiment was conducted to compare the effects of persulfate with gamma radiation on the disinfection efficiencies against Escherichia coli K12. The microorganism used for the disinfection experiments were prepared by transferring a bacterial stock culture into a 50 ml nutrient broth an incubating for 24 hrs at $37^{\circ}C$. The initial concentration of the harvested culture was approximately $10^7$ to $10^9CFU\;ml^{-1}$. The culture solution was irradiated at different absorbed doses of 0.1, 0.2, 0.3, 0.4, 0.5, 0.7, and 1 kGy, respectively. The disinfection efficiency of persulfate with gamma radiation of 0.3 kGy against Escherichia coli K12 was 97.2% and while the gamma radiation only was 90.01% at 0.3 kGy. Therefore, it could be thought that addition of persulfate in the disinfection of Escherichia coli K12 can enhance the disinfection efficiency when it is used together with gamma radiation.

TNF-α, IL-6, IL-8 저해를 통한 천연약용식물 추출물의 항염증 특성 (Anti-inflammatory Activity of Herbal Extracts through Inhibition of TNF-α, IL-6 and IL-8)

  • 김용수;권희정;박종석;임윤묵;노영창
    • 방사선산업학회지
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    • 제5권3호
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    • pp.273-277
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    • 2011
  • This study evaluated the cytotoxicity and anti-inflammatory activities of natural herbal extracts including Houttuynia cordata and Eucommia ulmoides against human mast cell (HMC-1). Houttuynia cordata (HC) and Eucommia ulmoides(EU) were extracted with distilled water (at $75^{\circ}C$) and then freeze-dried for 5 days. Finally, the mixture of HC and EU were sterilized by ${\gamma}$-rays irradiation. Cytotoxicity of the mixture against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory mediator cytokines such as TNF-${\alpha}$, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showed that mixture of HC and EU had no cytoxicity and reduced TNF-${\alpha}$, IL-6, IL-8 response on HMC-1 cells.

감마선 조사된 채취 미역의 유전독성학적 안전성 평가 (Genotoxicological Safety of Undaria pinnatifida Gamma-irradiated after Harvest)

  • 최종일;김재훈;송범석;김재경;이주운;전병수;안동현;변명우;박경숙
    • 방사선산업학회지
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    • 제5권1호
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    • pp.81-86
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    • 2011
  • This study was conducted to evaluate the genotoxcity of gamma-irradiated Undaria pinnadifida after harvest for enhancing the storage and microbial safety during drying process. The harvested seaweed was irradiated with the doses of 1 and 10 kGy. The genotoxicity was evaluated by Ames test (Salmonella typhimurium reversion assay) and SOS chromotest. The results from all samples were negative in the bacterial reversion assay with S. typhimurium TA98 and TA100. No mutagenicity was detected in the assay, at both with and without metabolic activation. The SOS chromotest also indicated that the gamma-irradiated U. pinnadifida did not show any mutagenicity. Therefore, this study indicated that gamma irradiation could be used for extending the shelf-life of U. pinnadifida.

감마선 조사된 미역으로 제조한 미역국의 관능 연구 (Sensory Evaluation of Korean Seaweed Soup, Miyukgook, with Gamma-irradiated Undaria pinnatifida)

  • 최종일;김재훈;송범석;김재경;이주운;전병수;안동현;변명우;박경숙
    • 방사선산업학회지
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    • 제5권1호
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    • pp.21-24
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    • 2011
  • This study was conducted to evaluated the sensory properties of Miyukgook, Korean seaweed soup, with gamma-irradiated Undaria pinnadifida for enhancing the storage of the harvested seaweed. The harvested seaweed was irradiated at the doses of 1, 3, 5, and 10 kGy. The Miyukgook was made by following the general recipe. The values of pH and acidity of the Miyukgook were not changed by the irradiation of harvested Undaria. The sensory evaluation result showed that the preference scores in all the sensory properties was a little decreased when it was irradiated, but sensory score of less than 5 kGy samples was similar in all terms. Therefore, it was considered that gamma irradiation with less than 5 kGy was effective for improvement of the storage stability of U. pinnatifida without the changes in the sensory of its major cooked food, Miyukgook.

미생물 발효 셀룰로오스와 젤라틴을 함유한 조직공학적 세포지지체의 제조 및 특성 (Preparation and Characterization of Gelatin Scaffold Containing Microorganism Fermented Cellulose)

  • 임윤묵;김미영;권희정;박종석;노영창;이병헌;이종대;송성기;김성호;최영훈;이선이
    • 방사선산업학회지
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    • 제4권4호
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    • pp.373-379
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    • 2010
  • Cellulose, chitin, chitosan and hyaluronic acid are well known as polysaccharides. These polysaccharides have many effects on cell growth and differentiation. Cell activation increases with increasing the polysaccharides concentration. In this study, gelatin scaffold containing microorganism fermented cellulose, citrus gel were prepared by using irradiation technique. Physical properties of the scaffolds were investigated as a function of the concentrations of gelatin and citrus gel and the cell attachment, cell morphology and inflammation of the scaffolds also were characterized for regeneration of skin tissue.

주요 산지별 제조 복분자주의 기호도 및 휘발성분 분석 (Volatile Analysis and Preference Measurement of Korean Black Raspberry Wines from Different Regions)

  • 이승주;이광근
    • 산업식품공학
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    • 제13권4호
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    • pp.302-307
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    • 2009
  • 국내 주요 복분자 산지인 고창, 정읍, 횡성, 순창산 복분자로 복분자주를 제조하여 제조 복분자주의 휘발성 향기성분 분석과 기호도 조사를 통해 산지별 차이를 파악하였다. 복분자주의 주요 향기성분으로 phenylethyl alcohol, isoamyl alcohol, diethyl succinate, isobutyl alcohol, acetic acid, octanoic acid, benzoic acid, $\alpha$-terpineol, myrtenol, pcymene-8-ol이 동정되었고 특히 적포도주에 비해 terpene류가 많이 검출되어 복분자주의 odor-active compounds를 분석하기 위해 gas-chromatography-olfactometry를 포함한 다양한 향기성분 분석 연구가 필요한 것으로 보인다. Ester류는 횡성산과 정읍산 복분자주에서 많이 검출되었고 alcohol과 acid류도 횡성산에서 가장 많은 농도를 나타내었다. Terpene류는 정읍산에서 가장 높게 나타났다. 차후 이들 시료에 대한 묘사분석을 통해 향기성분과의 상관관계 분석이 필요하리라 여겨진다. 전반적인 기호도에서는 제조복분자주에서 고창, 순창, 횡성, 정읍순으로 나타났고 특히 횡성과 정읍 시료의 경우 단맛은 약하고 신맛은 너무 강한 것으로 나타나 당산비 조절이 필요한 것으로 여겨진다.

전자선 조사에 따른 폴리이미드 필름의 유전특성 변화 (Effects of Electron Beam Irradiation on the Dielectric Properties of Polyimide Films)

  • 김현빈;전준표;강필현
    • 방사선산업학회지
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    • 제4권3호
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    • pp.285-288
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    • 2010
  • Polyimide films have excellent thermal stability, reliable mechanical properties and low dielectric constant. Therefore, this material is widely used in many industrial fields such as microelectronics, flexible circuits, semiconductor products and aerospace materials. In space applications, earth-orbiting hardware operates in environments that generally include neutral particles, charged particles such as trapped protons and electrons, solar protons, and cosmic rays. Under these conditions, polyimide films were changed in the optical, electrical and mechanical properties. Therefore, in this study, we evaluated the effects of electron beam irradiation on polyimide. The O-H functional groups were created on the polyimide film surface in the results of FT-IR spectra. And it was found that the dielectric constants were changed as a function of electron beam dose.

방사선그라프팅 음이온교환막의 기계적 물성에 대한 4차 암모늄 음이온교환기의 영향 (Influence of Quaternary Ammonium Anion Exchange Moieties onto Mechanical Properties of Radiation-grafting Anion Exchange Membranes)

  • 고범석;손준용;신준화
    • 방사선산업학회지
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    • 제6권1호
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    • pp.49-54
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    • 2012
  • Various anion exchange membranes were prepared by radiation graft copolymerization of vinylbenzyl chloride onto fluorinated films and subsequent quaternization with various tertiary amines such as trimethylamine, N,N-dimethylbuthylamine, N,N-dimethylaniline, and N-methylpiperidine. The quaternizations of the anion exchange membranes were confirmed by measuring of the ion exchange capacities of the membranes. The mechanical properties and the water uptakes were also measured. The elongation at break was found to be largely dependent on the fluorinated film, the quateranry ammonium, and the degree of grafting. The results indicate that the poly (ethylene-alt-tetrafluoroethylene) with quaternized trimethylamine moiety exhibits higher flexible property compared to the other prepared anion exchange membranes.