• Korean Journal of Clinical Laboratory Science
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• v.47 no.3
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• pp.105-111
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• 2015

In late December 2013, the Ebola virus emerged from West Africa. The outbreak started in Guinea and rapidly spread to Liberia and Sierra Leone. Initially, the virus is spread to the human population after contact with infected wildlife and then spread person-to-person through direct contact with body fluids such as blood, sweat, urine, semen, and breast milk. The Ebola virus infects endothelial cells, mononuclear phagocytes and hepatocytes. It causes massive damage to internal tissues and organs, such as blood vessels and the liver, and ultimately death. Most tests for the virus RNA rely on a technology called reverse-transcriptase polymerase chain reaction (RT-PCR). While this method is highly sensitive, it is also expensive, requiring skilled scientists, and delicate power supplies. The strip analytical technique (enzyme-linked immunosorbent assay or ELISA) detects antigens or antibodies to the Ebola virus. This test is cheap and does not require electricity or refrigeration. Despite ongoing efforts directed at experimental treatments and vaccine development, current medical work on the Ebola viral disease is largely limited to supportive therapy. Thus, rapid and reliable diagnoses of the Ebola virus are critically important for patient management, infections, prevention, and control measures.

• Journal of Embryo Transfer
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• v.24 no.1
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• pp.29-32
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• 2009

Prostaglandin $F_2{\alpha}$ ($PGF_2{\alpha}$) can facilitate release of epinephrine from the adrenal gland. The objective was to extend these findings and determine the effects of $PGF_2{\alpha}$ on sexual activity and semen collection training in sexually inexperienced boars. Boars (n=32; $281{\pm}18$ days of age) were moved individually once weekly to a semen collection room equipped with an artificial sow. Before entering the semen collection room, boar received i.m. treatments of $PGF_2{\alpha}$ at doses of 5 (n=8), 10 (n=8), or 20 (n=8), and control boar (n=8) were not treated. Reaction time (elapsed time after entering collection pen until the start of mounting) for boars receiving 5mg ($3.3{\pm}0.9\;min$), 10mg ($3.3{\pm}0.8\;min$) $PGF_2{\alpha}$ was shorter (p<0.05) than for controls ($6.7{\pm}0.9min$). Duration of ejaculation (min) per session was longer (p<0.05) for $PGF_2{\alpha}$ (10 mg, 20 mg)-treated boars ($7.3{\pm}0.7\;min$, $6.9{\pm}0.7\;min$), compared to control ($3.4{\pm}0.8\;min$). The number of training session per boars was less (p=0.056) for $PGF_2{\alpha}$ 10mg-treated boars ($1.0{\pm}0.4$), compared to control ($2.0{\pm}0.4$). Semen characteristic such as volume, concentration, the number of total ejaculated sperm, were similar for $PGF_2{\alpha}$-treated and controls. There was no apparent difference on sperm movement characteristics (Mot: motility, VCL : curve linear velocity, VSL : straight line velocity, VAP : average path velocity, LIN : linearity) after semen preservation by collected with or without $PGF_2{\alpha}$ treatment. In summary, administration of $PGF_2{\alpha}$ in boars increased the sexual activity and facilitated the training boars to mount an artificial sow for semen collection, but did not affect semen characteristic.

• Journal of Embryo Transfer
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• v.27 no.1
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• pp.51-55
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• 2012

The objective of this study was to investigate the motility and kinematics of boar sperm that while stored at 4C. The samples of fresh boar semen were place into an extender, Androhep, and stored at $4^{\circ}C$. In three of these samples, cryoprotectants were added. The sperm's motilities and kinematics were evaluated by using microscope (${\times}400$) and the viability status was evaluated by using with eosin staining method. The 5 sample groups are; Goup A:Androhep (extender), stored at $17^{\circ}C$. Group B:Androhep (extender), stored at $4^{\circ}C$. Group C:Androhep (extender), + 3% glycerol (cryoprotectant), stored at $4^{\circ}C$. Group D:Androhep (extender), + 3% DMSO (cryoprotectant), stored at $4^{\circ}C$. Group E:Androhep (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$. In group A, the sperm's motility was reduced. On day one the sperm's motility was ($85.7{\pm}2.3$) and day 5 the motility was ($43.9{\pm}3.3$). In group B, C and D the sperm's motility were reduced to 0 on day 5. In group E the sperm's percentage of motility decreased. On day one the sperm's motility was ($42.0{\pm}0.5$) and day 5 the motility was ($2.3{\pm}0.3$). When comparing cryoprotectant in samples of boar sperm there is a slight improvement in the results when the use of Androhep Lite (extender), + 3% ethylene glycol (cryoprotectant), stored at $4^{\circ}C$ are used. Based on these results, ethylene glycol can protect sperm from heat shock at $4^{\circ}C$, but not satisfactory level. However, it showed the possibilities of liquid semen preservation at $4^{\circ}C$ by using cryoprotectant.

• Journal of Embryo Transfer
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• v.27 no.1
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• pp.29-35
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• 2012

The purpose of this study was to examine the effect of growing stages of the Korean Native Striped Bull (KNSB) on the freezability and fertility of frozen-thawed semen. First, we investigated the total motility (TM) and progressive motility (PM) according to the diluent used for semen freezing. Second, we examined the effect of the age of KNSB on semen volume, TM and PM of fresh and frozen-thawed semen. Third, we examined the effect of frozen semen from the different age of KNSB on the $in-vitro$ fertilization rate, and the artificial insemination pregnancy rate. The diluents used in this experiment were Triladyl$^{(R)}$ and Tris-egg yolk extender (EYE). Semen was collected from 5 KNSB in the growing stage (15 months) and 5 adult KNSB (36 months). When Triladyl or Tris-EYE extender was used for semen freezing, there was no difference of the mean TM and the mean PM. However, the mean TM was significantly higher in Bull No. 1885 than Bull No. 4283 ($p$ <0.05). The mean volume of semen collected from the 15-month-old bulls (2.3 ml) was significantly lower ($p$ <0.05) than that from the 36-month-old bulls (5.0 ml). The mean semen concentration was similar for the 15-month-old ($2.1{\times}10^9$ spermatozoa/ml) and 36-month-old ($1.8{\times}10^9$ spermatozoa/ml) bulls. For the 15-month-old and 36-month-old bulls, the mean TM of fresh semen were 93.7% and 88.3%, respectively, and the mean PM were 97.0% and 88.3%, respectively; the 15-month-old bulls showed a particularly high PM ($p$ <0.05). For the 15-month-old and 36-month-old bulls, the mean TM (56.0% and 58.0%, respectively) and the mean PM (64.0% and 70.7%, respectively) of frozen-thawed semen did not differ. The development rates of embryos after $in-vitro$ fertilization and the pregnancy rate after artificial insemination using frozen-thawed semen did not differ according to the bull's age. In summary, semen volume differed according to the bull's age, but semen concentration and survival rate, the $in-vitro$ fertilization rate, and the pregnancy rate did not differ according to the stripe bull's age. Accordingly, semen from bulls in the growing stage can be collected and frozen for the preservation and multiplication of rare livestock.

• Journal of Embryo Transfer
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• v.33 no.1
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• pp.23-30
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• 2018

The purpose of this study was to investigate the effects of the concentration of seminal plasma in aerobic and anaerobic conditions on the total motility(TM) and the progressive motility(PM) of spermatozoa in long term preservation of cooled equine semen. We also examine the pregnancy rates after artificial insemination using fresh, cooled or frozen semen, and different durations of cooled-preserved equine semen. In the aerobic state of cooled-preserved semen, As the increase of preserved duration to 24h, 48h, 72h, and 96h, TM tended to decrease in each of different concentrations of formalin-containing experimental group, TM tended to decrease regardless of the concentrations of SP. In different concentrations of SP, TM of without seminal plasma(SP W/O) group tended to be higher than that of SP 20%, SP 33% and SP 50%, especially TM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). PM was higher in the groups of SP W/O and SP 20% than in the groups of SP 33% and SP 50% from 24 h to 72 h in cooled-preservation, especially PM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). In the anaerobic condition of cooled-preserved semen, the results of TM and PM at different concentrations of SP were similar to the results in the aerobic condition although there was a difference in the ratio. The pregnancy rates of fresh-cooled, cooled-preserved and frozen semen were 66.3%, 60.7% and 34.5%, respectively, and the pregnancy rate of frozen semen was the lowest. We also found that it is possible to pregnancy after artificial insemination using 72 h cooled-preserved equine semen. There was similar of the pregnancy rates in the different month from April to August.

• Journal of Life Science
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• v.20 no.11
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• pp.1634-1639
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• 2010

This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at $10^{\circ}C$ and $13^{\circ}C$. More than 93% of the male population was produced by this method and growth of hormone-treated fish at $13^{\circ}C$ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least $3,600\;erg/cm^2$ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at $28^{\circ}C$ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.

• Journal of Digital Contents Society
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• v.12 no.1
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• pp.23-32
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• 2011

For the past few years, unlike wireless widespread United States and Japan, expensive cost and platform of the mobile web carrier underground and self-centered made achievement of domestic mobile web market sluggish. Nowadays, more and more people pay attention to the mobile web service because of the increased quality of wireless internet, development of mobile device and variety of the flat-rate payment system. However, another big problem is that mobile web is too hard to use. Also, the poor content of mobile web service is an extremely serious problem. In this paper, we put forward a new method for easy to access mobile web service which is use WINC expansion numbers and users can use these numbers to shit their own website. Along with the benefits of purchase WINC number general users are able to make website and control their website at less time and energy.

• Journal of Digital Contents Society
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• v.16 no.1
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• pp.137-144
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• 2015

This study tried to anticipate the near future changes of domestic online video, music service and media usage through the status quo. The research group was focused on the twenties, University students because they are the active media contents users. It surveyed the students in Busan, and used the method of descriptive statistics analysis for the understanding of the present state and near future prospect. This study shows that almost half of them use both services, and three people out of ten are the heavy users who use the services for over 3 hours a week. The streaming method is higher proportion than download method in using type. They are getting accustomed to pay contents cost, but it couldn't be said the paying content cost has been established. Preferred contents genre appears to be the RMC(Ready Made Contents) such as existing broadcaster contents and movies. Notable result on media usage was the proportion of the so-called 'Zero-TV'. It was 32%, significantly higher than the proportion of the total population(4.4%). Integrating the analysis results, we can expect that the usage pattern will be changed gradually, thus the advent of various revenue models will emerge.

• Polymer(Korea)
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• v.35 no.2
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• pp.113-118
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• 2011

Solid dispersion is used to improve the solubility of water-insoluble drug. Release properties depend on the characteristics of polymer and the physicochemical properties of solid dispersion. In this study the solid dispersions of ibuprofen and cellulose acetate were prepared using spray-drying and rotary evaporation. The physicochemical properties of the solid dispersions were analyzed by SEM, XRD, DSC, and FTIR. The hydrophilicity of polymer was analyzed by measuring the contact angle of water. The results of DSC and XRD analysis demonstrated that the crystallinity of ibuprofen was changed by solid dispersion preparation. The results of contact angle showed that hydrophilicity was proportional to polymer content. Release profile showed that for solid dispersion. the release rate of ibuprofen decreased as polymer content increased in intestinal juice (pH 6.8). The dissolution rate of ibuprofen was improved with increasing polymer content in gastric juice (pH 1.2).

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.60-60
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• 1961

Insulin, Glucagon등의 홀몬과 Reserpine 및 Nicotine의 투여(投與)가 자우췌장(仔牛膵臟) Iangerhans도도(島嶋) 내분비세포(內分泌細胞)들에 미치는 현미경적(顯微鏡的) 및 전자현미경적(電子顯微鏡的) 영향(影響)을 연구(硏究)하는 도중 굉장히 큰 Iangerhans 도도(島嶋)들을 관찰하고 그 조직학적(組織學的) 세포학적(細胞學的) 특징(特徵)과 그 분포상태(分布狀態)를 구명(究明)하고저 한것이다. 연령(年齡) 2내지(乃至)4개월(個月)된 10두(頭) Holstein자우(仔牛)에서 췌장(膵臟)의 두부(頭部), 중간부(中間部) 및 미부(尾部)를 떼어서 Bouin 씨고정액(氏固定液)에 고정(固定)하고 파라핀절편(切片)을 만들어 Gomori의 Aldehyde Fuchsin stain에 의해서 Iangerhans도도세포(鳥鴨細胞)들을 염색(染色)하여 관찰하였다. 총괄(總括) 및 고찰(考察) 1. 지금까지 보고(報告)된 최대(最大)의 Iangerhans도도(島嶋)는 개에서 관찰된 333u 이었는데 본연구(本硏究)에서는 직경(直徑) 1,395u의 거대(巨大)한 도도(島嶋)를 관찰하였고 기리 2,700u에 달하는것을 보았다. 2. 췌장각부위별(膵臟各部位別)의 도도(島嶋)의 분포상태(分布狀態)는 다른 연구보고(硏究報告)들에 대체로 일치(一致)하였으나 그수(數)는 50평방(平方)mm에 평균(平均) 191개(個)로서 Trautmann이 보고(報告)한 125개(個)보다 66개(個)나 더 많은 수(數)이었고 직경(直徑) 200u이상(以上)에 달하는 도도(島嶋)의 백분율(百分率)은 평균(平均) 1.8%이었다. 3. 거대(巨大)한 Iangerhans도도(島嶋)은 거이 전부(全部)가 Beta세포(細胞)들로만 구성되어 있으므로 거대(巨大)Beta도도(島嶋)라고 이름지었으며 비록 그 수(數)는 적지만 한개의 거대(巨大)Beta도도(島嶋)가 차지하는 용량(容量)은 보통 크기의 도도(島嶋)가 차지하는 용량(容量)의 수백배(數百倍)에 달할것이므로 우췌장(牛膵臟)의 Insulin분비(分泌)를 연구(硏究)할 때에는 반듯이 이사실(事實)을 고려(考慮)해야 할것으로 생각된다. 4. 거대(巨大)Beta도도(島嶋)의 간질결합조직지주(間質結合組織支柱)는 매우 현저(顯著)하며 그 속에는 비교적 큰 동맥(動脈)과 정맥혈관(靜脈血管) 그리고 개재도관양(介在導管樣) 조직(組織)들이 있으며 거대(巨大)Beta도도(島嶋)들은 출생후(出生後) Iangerhans도도내외(島嶋內外)에 밀접하게 위치(位置)하고 있는 외분비(外分泌) 말초도세포(末梢導細胞)들이 증식(增殖)하여서 형성(形成)되는 것으로 믿어진다. 5. 거대(巨大)Beta도도(島嶋)의 Beta세포(細胞)들은 매우 뚜렷한 Negative Colgi images를 보이며 포장핵(胞狀核)과 비대(肥大)한 핵소고(核小高)들은 이 세포(細胞)들이 보통 도도(島嶋)의 Beta세포(細胞)들에 비(比)해서 더 활발(活發)한 분비작용(分泌作用)을 하고 있음을 시사(示唆)하는 것이다.