• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.76-76
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• 2019

본 연구에서는 강원도 고성군 죽왕면 구성리에 위치한 명우산(335.5m)과 오봉리에 위치한 오음산(281.7m) 일대를 중심으로 식물상을 조사하였다. 명우산의 관속식물은 72과 203속 236종 4아종 23변종 3품종으로 총 239분류군이 조사되었고, 오음산의 관속식물은 71과 185속 236종 2아종 31변종 3품종으로 총 257분류군이 조사되었다. 주요 식물로 명우산의 경우 특산식물은 노랑갈퀴(Vicia chosenensis), 백운산원추리(Hemerocallis hakuunensis) 등 4분류군이 조사되었고, 이는 명우산에서 조사된 식물의 1.67%로 특산식물 360분류군(정규영 등, 2017)의 1.1%에 해당된다. 오음산의 경우 특산식물은 노랑갈퀴(Vicia chosenensis), 새며느리밥풀(Melampyrum setaceum var. nakaianum) 2분류군이 조사되었고, 이는 오음산에서 조사된 식물의 0.78%, 특산식물 360분류군(정규영 등, 2017)의 0.56%에 해당된다. 희귀식물로는 명우산은 확인되지 않았고, 오음산은 위기종(EN)인 참작약(Paeonia lactiflora var. trichocarpa) 1분류군이 확인되었다. 침입외래식물로는 명우산의 경우 단풍잎돼지풀(Ambrosia trifida), 미국쑥부쟁이(Aster pilosus) 등 총 10분류군이 확인되었고, 오음산의 경우 미국실새삼(Cuscuta pentagona), 돼지풀(Ambrosia artemisiifolia) 등 총 12분류군이 확인되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.72-72
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• 2019

간무봉(555.8m)은 강원도 인제군 남면에 위치하고 있다. 북한강의 지류인 소양강으로 대부분 둘러싸여 있으며 북서쪽에 봉화산(874.4m), 북동쪽에 기룡산(940.4m)과 이어진다. 현재까지 간무봉을 대상으로 한 식물상 조사 이력은 없었으나, DMZ 일원에 위치하는 산이기 때문에 DMZ의 식물상 연구를 위한 기초 자료로 보전하고 탐색하는 의미가 있다. 현지조사는 2018년 4월부터 10월까지 총 6회 수행하였다. 간무봉에는 굴참나무, 신갈나무, 소나무가 우점하고 있는 형태였으며, 조사 결과 관속식물은 73과 175속 211종 3아종 27변종 4품종으로 총 245분류군이 조사되었다. 그 중 양치식물은 고비, 고사리삼, 십자고사리, 퍼진고사리 4분류군이고, 나자식물은 소나무, 일본잎갈나무 2분류군, 피자식물은 노랑제비꽃, 은방울꽃, 꽃향유 등 205분류군이었다. 이는 우리나라 관속식물 4,494분류군(국립수목원, 2016)의 5.45%에 해당한다. 간무봉에서 확인한 특산식물은 진범, 할미밀망, 갈퀴아재비, 병꽃나무 등 6 분류군이며, 희귀식물은 미치광이풀(LC), 말나리(LC) 2 분류군 이외에 VU 이상에 해당하는 희귀식물은 발견하지 못하였다. 그리고 침입외래식물(국립수목원, 2016)의 경우 미국자리공, 수박풀, 미국쑥부쟁이, 단풍잎돼지풀 등 17 분류군이었다. 간무봉 주변에는 경작지, 군부대 훈련장소 등이 있으며, 서쪽 사면은 이미 벌목이 많이 된 상태이며, 북동쪽도 마을과 더불어 경작지로 개간되어 있다. 따라서 주변의 외부에서 가해지는 인위적인 간섭이 많은 상태라고 볼 수 있으며, 이미 유입된 단풍잎돼지풀 등 생태계교란종에 의한 변화에도 유의할 필요가 있다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.77-77
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• 2019

본 연구는 DMZ일원에서 생육하고 있는 자생식물에 대하여 우선순위를 설정하고, DMZ일원 종다양성의 지속적인 건강성 유지 및 보전을 위한 중장기 모니터링 기술을 개발하기 위하여 중점관리종을 선정하고자 하였다. 또한, 이를 토대로 DMZ지역과 북방계지역에 대한 생물자원을 연구할 수 있는 기본틀을 마련하고 생물권보전지역을 지정함으로써 국제교류 및 이해에 기여하는 연구를 수행하였다. 현재 DMZ일원의 관속식물상은 157과 754속 2,047종 12아종 340변종 105품종 총 2,504분류군이 생육하고 있다. 이 중 희귀식물은 총 285분류군, 특산식물은 총 124분류군, 침입외래식물은 130분류군이 분포하고 있다. 전체 2,504분류군 중 희귀식물, 특산식물, 침입외래식물을 대상으로 전국분포정보 및 DMZ일원 분포정보를 수집하였다. 이를 토대로 희귀 특산식물에 해당하는 보호종 선정을 위한 평가지표는 IUCN 적색목록기준 의 국내수준 및 DMZ일원의 희귀 특산식물의 등급을 CR종 3점, EN종 2점 VU종 1점으로 점수를 평가한 뒤 국내 분포 지역수(IUCN red list criteria B)에 대한 가중치를 적용하여 보호우선순위를(I$10,576.9km^2$) 대비 종 분포면적의 0~20% 1점, 21~40% 1.5점, 41~60% 2점, 61~80% 2.5점, 81~100% 3점으로 분포등급을 평가한 뒤 생태교란종(14분류군) 및 산림침입종(181분류군)에 대한 가중치를 적용하여 관리우선순위를(I

• Korean Journal of Ecology and Environment
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• v.44 no.3
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• pp.253-263
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• 2011

This study was conducted to gain preliminary data for restoration and management of constructed small-scale ponds in paddy fields through analysis of their physicochemical and biological properties. A field survey was performed at 13 small-scale ponds located in paddy fields from August 2009 to October 2010. Structural properties, water quality, soil characteristics and fish fauna were measured. Results showed that small-scale ponds without frames might lose their function over time because of crumbling walls. Therefore, it is necessary for these ponds to have frames for soil protection and sustainable maintenance. Chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) concentration were higher than the water quality standard for agricultural water in small-scale ponds. In particular, TN concentration was 8.03 mg $L^{-1}$ and over 8 times the water quality standard because of the presence of livestock such as cows and pigs in the study areas. Sand, organic matter and available phosphorus contents of soil in small-scale ponds was 53.4${\pm}$16.6%, 21.8${\pm}$9.74 g $kg^{-1}$ and 12.8${\pm}$7.59 mg $kg^{-1}$, respectively indicating that sand and available phosphorus contents were suitable for plants in small-scale ponds, but organic matter contents was somewhat low in newly constructed small-scale ponds, and would take some time to stabilize for plant growing. Fish fauna was not diverse with only 4 species at all sites surveyed. Collected fishes share a common feature that they all inhabit paddy fields or canals with shallow water depth. In this study, all ponds were not linked to the streams and canals around them. It appears that connection to adjacent streams was the major factor controlling fish fauna in small-scale ponds. The results of statistical analysis were classified into three groups. Factor 1 was 26.3%, which shows a structural properties such as area and depth of small-scale pond. As for factor 2, it appears on 20.1%, showing water quality like a TP, suspended solids (SS) and COD. Small-scale ponds were classified into three groups by factor scores. Group I consisted of 6 small-scale ponds, which were larger than the others. Group III had higher water quality than the others. We conclude that the most important points to be considered for restoration and management of small-scale ponds is connection with adjacent streams or ditches and depth and size of the small-scale pond.

• Research in Plant Disease
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• v.17 no.3
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• pp.342-350
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• 2011

Throughout the years 2008 to 2010, we analyzed approximately two thousand oriental melon samples collected from Seongju, using electron microscopy and testing by RT-PCR using primers specific for eight cucurbit-infecting viruses. Data from RT-PCR indicated that Cucumber green mottle mosaic virus (CGMMV), Watermelon mosaic virus 2 (WMV2) and Zucchini yellow mosaic virus (ZYMV) were present and the other viruses were not detected. Among them, CGMMV and WMV2 were the most prevalent pathogens. CGMMV was thought to infect oriental melon from the early growing season, and reached nearly 100% in the later of growing period. Otherwise, WMV2 emerged from June, several months later compared to CGMMV. CGMMV was detected from all aerial parts of the oriental melon including seeds, but not from the roots of the grafted pumpkin rootstock. Seed of two out of five commercial varieties were shown to be CGMMV positive. Nine varieties of pumpkins used as rootstocks were not infected with CGMMV. When the seedlings of grafted oriental melon were transplanted into pots mixed with the oriental melon debris infected with CGMMV, they were not infected by CGMMV. Cutting of pruning shear and the contact of tendrils contributed 48% and 30% to the transmission of the virus, respectively.

• Research in Plant Disease
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• v.17 no.3
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• pp.334-341
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• 2011

The kinds of crop requested from agricultural actual places of famers, Agricultural extension services and so forth was 8 including red pepper for vegetables, 4 including apple for fruit trees and 6 including chrysanthemum for flowers in 2010. The important vegetables in clinical diagnosis of viral diseases were tomato, watermelon and red pepper having the requested rate of 31.8%, 21.4% and 19.5%, respectively. On fruit trees, grape and apple were most common with the requested rate of 63.6% and 33.0%, orderly. On floral crops, tulip and cactus were damaged by viral diseases with the requested rate of 60.0% and 20.0%, orderly. On peppers and tomatoes, six viruses including Cucumber mosaic virus (CMV) and Broad bean wilt virus 2 (BBWV2) infected. Five viruses including Melon necrotic spot virus (MNSV) and CMV were identified from watermelons. On grapes, six viruses including Grapevine fleck virus (GFkV) infected. CMV was identified from six vegetables including pepper out of 8 kinds of vegetables and tulip plant. Total agents of virus and viroid species were 32 and 4 species, respectively, in 2010. Tomato yellow leaf curl disease by Tomato yellow leaf curl virus and Tobacco yellow leaf curl virus was occurred newly at 18 Si/Gun areas including Buan, Jeonbuk province in 2010 and the total areas were increased up to 58 Si/Gun from the first incidence in 2008. Tomato spotted wilt virus (TSWV) occurred newly at two areas of Jinan, Jeonbuk and Jeju in Jeju province in 2010, and the incidence areas were expanded to 25 Si/Gun areas from severe occurrence at Anyang area in 2004. No incidence of TSWV was recorded only in Gyeongbuk and Chungbuk province. Tomato bushy stunt virus occurred newly at Jinju, Gyeongnam, and it had the total incidence areas of 5 Si/Gun after first observation at Sacheon, Gyeongnam in 2004.

• Research in Plant Disease
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• v.16 no.3
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• pp.238-246
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• 2010

In 2006 fall, a preliminary survey of viruses in two important medicinal plants, Cynanchum wilfordii and C. auriculatum, was conducted on the experimental fields at the Agricultural Research and Extension Services of Chungbuk province in Korea. On each experimental fields, percentage of virus infection was ranged from 20 to 80%, and especially an average of disease incidence propagated by roots was twice higher than that by seeds. The various symptoms were observed in Cynanchum spp. plants, such as mosaic, mottle, necrosis, yellowing, chlorotic spot and malformation etc. In electron microscopic examination of crude sap extracts, filamentous rod particles with 390-730 nm were observed in most samples. The virus particles were purified from the leaves of C. wilfordii with typical mosaic symptom, and the viral RNA was extracted from this sample containing 430-845 nm long filamentous rod. To identify the viruses, reverse transcription followed by PCR with random primers was carried out. The putative sequences of P3 and coat protein of potyvirus were obtained. From a BLAST of the two sequences, they showed 26-38% and 62-72% identities to potyviruses, respectively. In SDS-PAGE analysis, the subunit of coat protein was approximately 30.3 kDa, close to the coat protein of potyvirus. In bioassay with 21 species in 7 families, Chenopodium quinoa showed local lesion on inoculated leave and chlorotic spot on upper leave, but the others were not infected. RT-PCR detection using specific primer of C. wilfordii and C. auriculatum samples, all of 24 samples with virus symptom was positive, and five out of seven samples without virus symptom were also positive. On the basis of these data, the virus could be considered as a new member of potyvirus. We suggested that the name of the virus was Keunjorong mosaic virus (KjMV) after the common Korean name of C. wilfordii.

• Journal of Korean Society for Atmospheric Environment
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• v.34 no.2
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• pp.306-320
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• 2018

Emission inventory is the essential component for improving the performance of air quality forecasting system. This study evaluated the simulated daily mean $PM_{2.5}$ concentrations in South Korea and China for 1-year period (Sept. 2016~Aug. 2017) using air quality forecasting system which was applied by the emission inventory of E2015 (predicted CAPSS 2015 for South Korea and KORUS 2015 v1 for the other regions). To identify the impacts of emissions on the simulated $PM_{2.5}$, the emission inventory replaced by E2010 (CAPSS 2010 and MIX 2010) were also applied under the same forecasting conditions. These results showed that simulated daily mean $PM_{2.5}$ concentrations had generally suitable performance with both emission data-sets for China (IOA>0.87, R>0.87) and South Korea (IOA>0.84, R>0.76). The impacts of the changes in emission inventories on simulated daily mean $PM_{2.5}$ concentrations were quantitatively estimated. In China, normalized mean bias (NMB) showed 5.5% and 26.8% under E2010 and E2015, respectively. The tendency of overestimated concentrations was larger in North Central and Southeast China than other regions under both E2010 and E2015. Seasonal differences of NMB were higher in non-winter season (28.3% (E2010)~39.3% (E2015)) than winter season (-0.5% (E2010)~8.0% (E2015)). In South Korea, NMB showed -5.4% and 2.8% for all days, but -15.2% and -11.2% for days below $40{\mu}g/m^3$ to minimize the impacts of long-range transport under E2010 and E2015, respectively. For all days, simulated $PM_{2.5}$ concentrations were overestimated in Seoul, Incheon, Southern part of Gyeonggi and Daejeon, and underestimated in other regions such as Jeonbuk, Ulsan, Busan and Gyeongnam, regardless of what emission inventories were applied. Our results suggest that the updated emission inventory, which reflects current status of emission amounts and spatio-temporal allocations, is needed for improving the performance of air quality forecasting.

• Research in Plant Disease
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• v.17 no.2
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• pp.196-204
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• 2011

Virus diseases occurring on cucumber was surveyed at main plantation areas of 'Sangju' and 'Gurye' in 2006 and 2007. Viral infection rate on cucumber was ranged from 14% to 90% in fields and the average infection rate was 46% at Sangju area. Cucumber cultivated at Gurye area had viral incidence ranged 9-100% and averaged 48%. The majorly infected viruses were Cucumber green mottle mosaic virus, Zucchini yellow mosaic virus, Papaya ring spot virus and Watermelon mosaic virus-2, and their infection rates were 23.5%, 13.0%, 9.0% and 2.0%, respectively in 2006 and 2007. Mixed infection rate of duplex, triplex and tetraplex was 31.5%, 7.5% and 2.5%, respectively. The rate of infection type was 47.7% by single infection, 31.5% by double infection and 88.7% by both infection types. Important viruses involved in mixed infection types were Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, Papaya ring spot virus and Broad been wilt virus2, orderly. Symptom in single infection was almost same on 'Dadagi' line and 'Chicheong' line of cucumber cultivars. Cucumber green mottle mosaic virus produced various symptoms of chlorotic spot, vein chlorosis and vein wrinkle and so on. Main symptoms of vein chlorosis, and severe mosaic and malformation were induced by Zucchini yellow mosaic virus. Mild symptoms were occurred relatively by Papaya ring spot virus and Watermelon mosaic virus2.

• Research in Plant Disease
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• v.17 no.1
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• pp.66-74
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• 2011

In 2005, a survey was conducted to identify virus diseases on victory onion, Allium victorialis var. platyphyllum grown in Ulleung island located in the East Sea. A total of 61 samples were collected from victory onion in the neighborhood of Seonginbong. The identification of viruses from the samples were carried out by electron microscopy and RT-PCR using primers species specific to GCLV, LYSV, SLV, OYDV and genus specific to Allexivirus, respectively. From sixty-one samples, filamentous rod particles (600-900 nm) were detected from four victory onion samples in EM, three samples containing SLV and one sample containing both SLV and Allexivirus in RT-PCR analysis, respectively. Victory onions naturally infected by the viruses were asymptomatic apparently. The viruses detected by RT-PCR were further characterized by the nucleotide sequence analysis of the coat protein region. Three isolates of SLV showed approximately 99% identities in the nucleotide and amino acid sequences, suggesting that they were likely to be the same strain. On the other hand, they showed approximately 75.7~83.7% identities in the nucleotide and 89.2~97.0% in amino acid sequences compared with the previously reported SLV isolates in Allium. The CP gene of the Allexivirus showed approximately 99.2% nucleotide identities and 98.8% amino acid identities with Garlic virus A. However, there was relatively low homology ranging from 60.6% to 81.5% compared with other Allexiviruses (GarV-C, GarV-E, GarV-X, GMbMV, and Shal-X). These data suggested that two viruses, SLV and GarV-A identified from victory onion, are named SLV-Ulleungdo and GarV-A-Ulleungdo, respectively. This is the first report of viruses infecting victory onion.