• Journal of the korean veterinary medical association
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• v.20 no.1
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• pp.49-61
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• 1984

The purpose of the experiment was to clarify morphologically normal growth pattern of male reproductive organ in heavy meat breed rooster. The observations were composed of normal growth in weight of whole body, comb, testis and pituitary glands and of hi

• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.331-339
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• 1998

The purpose of this study was to investigate the applicability of periodic acid thiocarbohy-drazide silver proteinate, physical development (PA-TCH-SP, -PD) stain to the seminiferous tubules for the ultrastructural studies of Sertoli cell column and Sertoli cell processes. In the Sertoli cell cloumn and Sertoli cell processes, high concentration of the reactive granules were observed under transmission electronmicroscope (TEM) after PA-TCH-SP-PD stain. Also some reactive granules were seen in the spermatogonium cytoplasm, clearly. These reactive granules specifically stained with PA-TCH-SP, -PD make the Sertoli cell column, Sertoli cell processes and spermatogonium cytoplasm easy to distinguish from nucleus of the germ cells, spermatocyte, spermatid and residual body which did not contain the reactive granules. This result indicates that the PA-TCH-SP, -PD stain is superior to other traditional electronic double stain methods for the ultrastructural studies of Sertoli cell.

• Journal of Korean Biological Nursing Science
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• v.1 no.1
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• pp.42-55
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• 1999

The purpose of this study was to investigate the effect of cyclophosphamide on the mouse seminiferous tubules by transmission electron microscopy at different groups: a control group, a group treated one time a week, and a group treated two times a week. Cyclophosphamide was injected in the intraperitoneal at a dosage of level 200mg/kg. The results obtained were as follows. 1. In the group of one time a week, pyknotic body and large vesicles were observed in cytoplasm of spermatogonium of seminiferous tubules, and in the intercellular space between spermatocytes, respectively. 2. In the group of two times a week, nucleus envelope in the spermatid was disrupted partially, observed vesicles in the nucleoplasm of spermatid, and separated or disrupted inner and outer membranes of mitochondria in the Sertoli cells.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.75-80
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• 2004

This study was performed to examine the cytotoxic effects of nicotine on the seminiferous tubules and Leydig cells in mice testis. A different amount of nicotine (2～15 mg/kg, for two weeks, one dose of 100 mg/kg) were administered to four-month male mice, and then the optical microscopic findings of its effect on testis of the mice are as follows: 1. The group that 2 mg/kg of nicotine was administered showed normal findings that nucleus and cytoplasm of Leydig cells are distinct, while the other group that 5 mg/kg of nicotine was given to showed nucleus and cytoplasm are swollen and thickened a little, and slightly dyed. 2. The group that 10 mg/kg of nicotine was given had irregular arrangement of spermatogenesis inside seminiferous tubules so it was impossible to distinguish phages of seminiferous tubules. It was also impossible to observe cells due to fusion of their nucleuses, and distinct cytoplasm. 3. The group that 15 mg/kg of nicotine was administered showed destruction of nucleuses and cytoplasm of spermatocytes and sperms and a fill of fibered connective tissues so that it is impossible to observe rumens of seminiferous tubules.

• Journal of Radiation Protection and Research
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• v.31 no.1
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• pp.31-35
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• 2006

For the purpose of the biological effect in black mouse by neutron irradiation, mice were irradiated with 16 or 32 Gy neutron (flux: 1.036739E+09) by tying flat pose at BNCT facility on HANARO Reactor. And 90 days later of irradiation, physical changes of testis and testis tissue were examined. There were no weight changes but a little bit volume changes and sperm counts in the testes. Atrophy of seminiferous tubules irradiated with 32 Gy neutron is increased in number and severity and those in stage VI showed depletion of spermatogonia and pachytene spermatocytes compared to the non-irradiated control group. Testis damage of black mouse was not recovered after long time by 32 Gy neutron irradiation.

• Applied Microscopy
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• v.38 no.3
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• pp.205-212
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• 2008

In this study morphological changes of the testes of golden hamsters treated with 6-aminonicotinamide (6-AN, 10 mg/kg body weight) in every two days were investigated using light and transmission electron microscopes. After the 7th injection of 6-AN, body weights of hamsters were significantly reduced, and the weight of testes were markedly reduced in the group of hamsters after 5th injections. Degeneration of seminiferous epithelium appeared first in the group receiving 5th injections, which were followed by severe degenerations after the 9th injection. In the degenerated seminiferous epithelium, deep vacuolization, and destruction of spermatogenic cells and Sertoli cells were also oberved. Multinuclear giant cells were also observed in the lumen of destructed seminiferous epithelium. But there were no edematous changes in the interstitial tissue, and the Leydig cells were found to be relatively intact. Therefore, these results show that 6-AN trigger severe morphological alteration of the spermatogenic cells and Sertoli cells, however Leydig cells are unaltered by 6-AN.

• Journal of Life Science
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• v.19 no.6
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• pp.799-803
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• 2009

The killing of male germ cells by radiation and other toxicants has recently been attributed to apoptosis, but a critical evaluation of the presence of the different features of apoptosis in each epithelial stage has not been performed. In this study, mouse testes exposed to radiation were examined by light microscopy and terminal transferase-mediated end labeling (TUNEL) with periodic acid-Schiff (PAS) stains to determine whether the cells were apoptotic according to several criteria. Apoptosis was easily recognized by the presence of peroxidase-stained, entirely apoptotic bodies. In the TUNEL-positive cells or bodies, the stained products correlated precisely with the typical morphologic characteristics of apoptosis as seen at the light microscopic level. The changes that occurred from 0 to 24 hours after exposing the mice to 2 Gy of gamma-rays (2 Gy/min) were examined. The numbers of apoptotic cells reached a peak at 12 hours after irradiation and then declined. The mice that received 0-8 Gy of gamma-rays were examined 8 hours after irradiation. Dose-response relationships were generated for each stage of the epithelial cycle by counting TUNEL-positive cells. The dose-response curves were linear- quadratic [y=(-0.014${\pm}$0.009)$D^{2}$+(0.31${\pm}$0.697)D+0.3575. Where y=the number of apoptotic cells per seminiferous tubule, and D=the irradiation dose in Gy, $r^{2}$=0.9] and there was a significant relationship between the frequency of apoptotic cells and the radiation dose. Although the maximum response was produced by 8 Gy, even 0.5 Gy induced marked changes. These changes were most pronounced in B spermatogonia of stage V and the spermatocyte at the mitotic cells of stage XII.

• Proceedings of the Korean Fiber Society Conference
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• 2002.04a
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• pp.429-432
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• 2002

잉크젯 프린팅 기술의 발전과 대형 플루터의 개발로 디지털 프린팅의 활용범위가 넓어지고 있으며, 특히 옥외광고용이나 배너용, 전시용으로의 사용이 확대되면서 종이와 같은 인쇄적성을 가지면서 사용목적에 맞는 물리적 특성을 지닌 다양한 제품들이 개발되고 있다. 이들 중 직물형태의 제품은 그 활용범위가 매우 넓지만 직물의 특성상 종이처럼 평활하지 못하고 직물 표면에서 잉크방울이 완전히 흡착되므로 잉크젯 프린터에 적합한 인쇄적성을 지니지 못해 최근에는 잉크젯 프린팅시 종이 표면과 근사한 최종 도트 사이즈를 얻기 위해 직물의 표면을 안료로 코팅하여 사용한다. (중략)

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.219-224
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• 2000

Reproductive biology of the naked-headed goby, Faronigobius gymnauchen was investigated by means of histological methods. The ovary was consisted of several ovarian lamellae and the oogonia originated from the inner surface of the ovarian lamella. The testis was seminiferous tubule One in internal structure. Seminiferous tubule was consisted of many testicular cysts which contained numerous germ cells in a same developmental stage. The size of group maturity was 4.5 cm intotal length. Gonadosomatic index(GSI) of the female and male was the highest in June and July, respectively. Reproductive cycle could be classified into the growing ($January{\~}March$), maturation ($April{\~}May$), ripe and spent (June{\~}July$), and recovery and resting ($August{\~}December$). Oocyte development was group-synchronous, and yolk nucleus was observed in the early growing oocyte.

• Korean Journal of Veterinary Research
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• v.41 no.1
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• pp.1-6
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• 2001

Pituitary adenyl ate cyclase activating polypeptide (PACAP) was originally isolated from the ovine hypothalamus and stimulated cAMP production in anterior pituitary cells. It is known that PACAP stimulates cAMP accumulation and contributes to the spermatogenesis and steroidogenesis in rat testis. The principal aim of this study is to determinate the distribution of PACAP mRNA and protein in adult rat testis. For this study, we used in situ hybridization and immunohistochemistry techniques in adult rat testis. PACAP mRNA was stage specifically expressed in seminiferous tubules. Positive signals of PACAP mRNA were detected in the developing germ cells at stages HI-VII of the epithelial cycle. The strongest signals of PACAP mRNA and protein were detected in round spermatids at stages V to early VII of the cycle. These results demonstrate that PACAP which is synthesised in the developing germ cells contributes to the spermatogenesis in rat testis. Thus, we suggest that PACAP plays a critical role in the function of testis.