• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.139-145
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• 2013

This study was carried out to determine the karyotype and chromosomal localizations of 45S and 5S rDNAs using FISH in Astragalus koraiensis. The somatic metaphase chromosome number of this species was 2n = 16 with basic chromosome number of x = 8. The karyotype of A. koraiensis was consisted of six pairs of median region chromosomes(chromosome 1, 3, 4, 5, 6, 8) and two pairs of submedian chromosomes(chromosome 2, 7). Based on the FISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 5. Whereas, two pair of 5S sites were detected on the short arm of chromosome 4 and centromeric region of chromosome 7, respectively. These are quite different patterns from A. membranaceus, A. membranaceus var. alpinus, and A. mongholicus. Although A. koraiensis is considered as Korean endemic species, therefore, it should be conducted out comparative FISH study with A. sikokianus and A. bhotanensis which are very similar to A. koraiensis morphologically.

• Korean Journal of Plant Taxonomy
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• v.44 no.3
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• pp.191-201
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• 2014

In order to find useful taxonomic morphological characters, 17 taxa from East-Asia Astragalus and Oxytropis anertii were investigated. In this study, seed coat structure, fruit surface, and fruit anatomical structures were selected to classify the species in Astragalus. As a results, useful morphological and anatomical characters in East-Asia Astragalus were life cycle, leaflet shape, raceme, petal color, seed color, pod shape, absent or present of hairs in pod surface, bent degree of dorsal suture, and thickness of endocarp. Using medicinal sources in China, A. floridus, A. membranaceus, A. membranaceus var. mongholicus and A. tongolensis were formed same group. However, these were not completely consensus in the morphological characters. The other hand, A. koraiensis and A. sikokianus, which have controversial taxonomic issue, have clearly no differences morphological characters each other. Therefore, A. koraiensis, A. sikokianus, and A. bhotanensis should be conducted a comparative study on the DNA sequences and molecular cytology in order to clarify the taxonomic treatments in the near future.

• Korean Journal of Plant Taxonomy
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• v.45 no.3
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• pp.227-238
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• 2015

Korean species within the genus Astragalus have been taxonomically revised based on herbarium specimens and field examinations. In this study, we recognized the following eight species and one variety: A. laxmannii subsp. laxmannii, A. dahuricus, A. sikokianus, A. uliginosus, A. schelichowii, A. setsureianus, A. mongholicus var. dahuricus, A. mongholicus var. nakaianus, and A. sinicus. Based on recent taxonomic progress with this genus, their scientific names are being reconsidered. A Korean plant, previously recorded as A. adsurgens, is included in the polymorphic taxa A. laxmannii subsp. laxmannii. Astragalus koraiensis was initially described from Gangwon Province as an endemic species, and it is now regarded as conspecific with A. sikokianus. Furthermore, the taxonomic entities and their morphological characteristics have been clarified for A. schelichowii and A. setsureianus, both of which are not well known in Korea. We provide a key to these species and enumerate their synonymies and taxonomic notes.

• The Hankook-Saengyark Bo
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• no.209
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• pp.5-5
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• 1996

• KSBB Journal
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• v.25 no.3
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• pp.271-276
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• 2010

This study was carried out to obtain the basic information for isoflavonoid contents that can be used to index Astragalus membranaceus B. cultivated in the Republic of Korea and China. Isoflavonoid contents in Astragalus membranaceus B. which were cultivated in various areas (Jecheon, Jeongseon, Yeongju, and Taebaek in Korea, and China) and ages (1-year-old, 3-years-old) were determined. Calycosin and formononetin as major constituents were determinated by HPLC method in Astragalus membranaceus B. The results show that there were no statistically significant differences for the average contents of isoflavonoids among 1-year-old and 3-years-old. However, isoflavonoid contents were significant differences according to the cultivation areas. HPLC analysis showed that the calycosin content of 1-year-old at Jeongseon was the highest level of $0.090{\pm}0.002%$ and that of 1-year-old at Yeongju was the lowest level of $0.010{\pm}0.001%$. The highest level of formononetin content was $0.050{\pm}0.001%$ of 1-year-old at China, while the lowest level was $0.020{\pm}0.001%$ of 1-year-old at Yeongju. These results strongly suggest that contents of isoflavonoid in Astragalus membranaceus B. might be quite different with respect to the cultivation areas.

• Journal of Plant Biology
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• v.24 no.1
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• pp.27-30
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• 1981

This paper is proposed to describe three new species, two varieties and one forma of flowering plants from central Korea and the Island of Cheju in southern Korea. The taxa of new plants are as follows: Astragalus koraiensis Y. Lee, sp. nov.; Astragalus nakaianus Y. Lee, sp. nov.; Commelina minor Y. Lee & Oh Y., sp. nov.; Cirsium rhinoceros Nakai var. albiflorum Y. Lee, var. nov.; Tripterospermum japonicum (Sieb. & Zucc.) Max. var. albiflorum Y. Lee, var. nov.; Adenophora remotiflora (Sieb. & Zucc.) Miq. form. albiflora Y. Lee, form. nov.

• Analytical Science and Technology
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• v.14 no.4
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• pp.311-316
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• 2001

We have investigated the trace amount in an oriental medicine in oder to determine the geographical origin by using inductively coupled plasma-atomic emission spectrometry(ICP-AES) and chemometric anlysis with principal component analysis(PCA) and pattern recognition. Astragali Radix from several agricultural places in Korea was selected as an example of the oriental medicine and analyzed by ICP-AES. The dried Astragali Radix sample was treated with $HNO_3$ and $H_2O_2$, then digested using microwave oven. Elements such as Mg, Al, K, Ca, Ti, Mn, Fe, Cu, Zn, and Ba with different concentrations were found an used for the identification of the origin of agriculture places. Especially, the concentration of Al, Fe, Zn and Ti were employed to investigate the relationship between. Astragali Radix and the agricultural places by PCA and pattern recognition. We have made a program that is based on chemometrics in analytical spectroscopy. The results of the chemometrics analysis indicated that a distinction among Yechon and Chechon, Chungson, Kurye and Chinese Astragali Radix could be made. We believe that principal component analysis(PCA) and pattern recognition is a valuable tool to identify the origin of Astragali Radix in terms of the agricultural place.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.3 s.58
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• pp.193-200
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• 2006

We investigated the effect of moisturizing and anti-oxidation of Astragalus membranaceus (Astragali Radix) root extract with respective to growing districts and extract methods for the purpose of development of cosmetic ingredients. Astragalii Radix was collected in Jecheon, Jeongseon, Taebaek, and Yeongju in Korea and China as growing districts. Formononetin was determinated by HPLC method as one of the various active agents in Astragalus membranaceus root extract. The 75% ethanol extract demonstrated to be more effective than $H_2O$-extracted one for a scavenging activities to DPPH radicals and reactive oxygens. The 75% ethanol extract showed $IC_{50}$ (50% scavenging concentration) of 2.162 mg/mL and 2.981 mg/mL in case of free radical scavenging activity test and reactive oxygen scavenging activity test, respectively Especially, free radical scavenging activity of isoflavonoids isolated from ethylacetate fraction was similar to scavenging activity of genistein. Astragalus membranaceus root extract of Jecheon district by sonicating extraction method was more effective in skin hydration compared with others.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.140-145
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• 1995

This experiment was carried out to investigate the effect of plant spacing on the growth and root yield at different harvesting year in Astragalus membranceus. A local variety collected in Jung Sun. Kang Won was used on the 30 and 40cm row spacing, with different spacing between plant of 5, 10, 15, 20cm respectively. Nitrogen, phosphorus and potassium fertilizers were applied as 6, 8 and 9kg/10a respectively, with compost of 1,000kg/10a. Branches, root length, root diameter and root weight were increased in the sparse planting compared with the dense planting. But emergence date, flowering date, stem height and node number were not significantly changed with plant spacing. The highest root yield was 211.8kg/10a at the $30{\time}10cm(67\;plants/m^2)$ in 1 year old plants of Astragalus membranceus. In case of two years old plants, branches, node number, root length, root diameter and root weight were increased in the sparse planting compared to the dense planting. But harvesting ratio was decreased in the dense planting, The highest dried root yield was 292kg/10a at the $40{\time}10cm\;(25\;Plants/m^2)$. In case of 3 years old plants, aerial part and root growth pattern were similar to the 2 years old plants, The dried root yield was highest(623kg/10a) in the $40{\time}10cm(25plants/m^2)$.