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Gene Expression as Related to Ripening in High Temperature during Different Coloration Stages of 'Haryejosaeng' and 'Shiranuhi' Mandarin Fruits (온주밀감 '하례조생'과 '부지화' 과실의 착색 단계별 고온에 의한 성숙 관련 유전자의 발현 변화)

  • Ahn, Soon Young;Kim, Seon Ae;Moon, Young-Eel;Yun, Hae Keun
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.665-676
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    • 2016
  • As high temperature during citrus growing season has caused a serious problems including inferior coloration in production of mandarins in Korea, we were to investigate the expression pattern of several genes related with coloration during the ripening in high temperature condition of citrus fruits. The expression of genes related with sugar metabolism, cell wall degradation, and flavonoid synthesis in high temperature conditions was investigated in fruits of 'Haryejosaeng' (Citrus unshiu) and 'Shiranuhi' mandarin (C. reticulata). While the expression of beta-amylase (BMY), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and flavanone 3-hydroxylase (F3H) was differently induced, expression of polygalacturonase (PG) decreased dependently on temperature conditions. In 'Haryejosaeng' mandarin, while the expression of genes related to the skin coloration, such as CHS and F3H genes increased at $25^{\circ}C$, the expression of PAL and stilbene synthase (STS) genes were induced at $30-35^{\circ}C$ in all ripening stages. In 'Shiranuhi' mandarin, the expression of the BMY gene decreased at early time point in all temperature condition and then increased at $30-35^{\circ}C$ than at $25^{\circ}C$ in the ripening stage 2 to 3 of fruits. F3H and STS genes also showed the tendency to decrease at $30-35^{\circ}C$. Although the expression levels of genes in ripening stage 1 and stage 2 of fruits showed similar patterns in both 'Haryejosaeng' and 'Shiranuhi', the expression levels of genes were down-regulated in late ripening stage of 'Shiranuhi' fruits compared to 'Haryejosaeng'. In general, the mRNA levels of seven tested genes were higher in 'Haryejosaeng' than in 'Shiranuhi' mandarin, and expression of genes by high temperature was regulated sensitively in 'Haryejosaeng' compared to 'Shiranuhi' mandarin. Further investigations of expression of various genes based on transcriptome analysis in early ripening stage can provide valuable information about the responses to climatic changes in ripening citrus fruits.

Influences of Site-specific N Application on Rice Grain Yield and Quality in Small Size Paddy Field (소규모 경작지에서 질소 변량시비가 벼 수량 및 품질에 미치는 영향)

  • Choi Min-Gyu;Choi Won-Young;Park Hong-Kyu;Nam Jeong-Kwon;Back Nam-Hyun;Lee Jun-Hee;Kim Sang-Su;Kim Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.5
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    • pp.369-378
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    • 2006
  • For precision farming the influences of site-specific N application on rice grain yield and quality were investigated in 0.5 ha paddy field from 2001 to 2003. In pre-cultured soil, EC, O.M., total nitrogen, phosphate and potassium content showed high spatial variation, ranging from 11.63 to 52.03% of coefficient of variation, while that of pH was relatively low. In rice growth characteristics, tiller number at panicle formation stage was more than 10% in coefficient of variation, but plant height, SPAD figure at panicle formation stage and milled rice yield, protein content in brown rice showed less below 10%. Spatial dependence was over 0.60 in pH, total nitrogen, phosphate and potassium in pre-cultured soil and was over 0.50 in plant height, SPAD figure and protein content, while it was below 0.22 in tiller number at panicle formation. The range of spatial dependence was longer than 20m in all factors except for protein content in brown rice. Basal dressing nitrogen rate was positively correlated with PH, $SiO_{2}$, plant height and SPAD figure. Nitrogen fertilization rate at panicle formation stage was positively correlated with EC and O.M.. Protein content in brown rice was positively correlated with $SiO_{2}$ in pre-cultured soil. Milled rice yield was positively correlated with plant height, tiller number and SPAD figure at panicle formation stage.

Proving Causation With Epidemiological Evidence in Tobacco Lawsuits (담배소송에서 역학적 증거에 의한 인과관계의 증명에 관한 소고)

  • Lee, Sun Goo
    • Journal of Preventive Medicine and Public Health
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    • v.49 no.2
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    • pp.80-96
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    • 2016
  • Recently, a series of lawsuits were filed in Korea claiming tort liability against tobacco companies. The Supreme Court has already issued decisions in some cases, while others are still pending. The primary issue in these cases is whether the epidemiological evidence submitted by the plaintiffs clearly proves the causal relationship between smoking and disease as required by civil law. Proving causation is difficult in tobacco lawsuits because factors other than smoking are involved in the development of a disease, and also because of the lapse of time between smoking and the manifestation of the disease. The Supreme Court (Supreme Court Decision, 2011Da22092, April 10, 2014) has imposed some limitations on using epidemiological evidence to prove causation in tobacco lawsuits filed by smokers and their family members, but these limitations should be reconsidered. First, the Court stated that a disease can be categorized as specific or non-specific, and for each disease type, causation can be proven by different types of evidence. However, the concept of specific diseases is not compatible with multifactor theory, which is generally accepted in the field of public health. Second, when the epidemiological association between the disease and the risk factor is proven to be significant, imposing additional burdens of proof on the plaintiff may considerably limit the plaintiff's right to recovery, but the Court required the plaintiffs to provide additional information such as health condition and lifestyle. Third, the Supreme Court is not giving greater weight to the evidential value of epidemiological study results because the Court focuses on the fact that these studies were group-level, not individual-level. However, group-level studies could still offer valuable information about individual members of the group, e.g., probability of causation.

The Occurrence of Rice Leaf-folder, Cnaphalocrocis medinalis (Lepidoptera : Crambidae) in Suwon and its Responses to Insecticides (혹명나방 개체군의 수원지역 발생 패턴 및 몇가지 약제에 대한 반응)

  • Park, Hong-Hyun;Cho, Jum-Rae;Park, Chang-Gyu;Kim, Kwang-Ho;Goh, Hyun-Gwan;Lee, Sang-Guei
    • Korean journal of applied entomology
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    • v.49 no.3
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    • pp.219-226
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    • 2010
  • This paper presents the occurrence and damage characteristics of the rice leaf-folder populations in the paddy fields of Dangsu-dong, Suwon from 2004 to 2007, and also reports the insecticide response of rice leaf-folder populations, which were collected from 2005 to 2006 in Korea and Vietnam. Laboratory measurements of the head capsule width and body length data enabled the identification of the rice leaf-folder larva stages collected in the field. The rice leaf-folder population in Suwon from 2004 to 2007 has a clear pattern consisting of two different group: the low and high density years. During the low density years (2004 and 2006), only one adult peak was noted in late August, with the damaged-hill percent less than 10% in late July, and the damaged-leaf percent around 2% in September. In contrast, during the high density years (2005 and 2007), two adult peaks were noted in early August and mid-September, with the damaged-hill percent was around 30% in late July, and the damaged-leaf percent 15 to 30% in September, which was beyond the economic injury level of rice leaf-folder. High correlations existed between the occurrence of rice leaf-folder in late July and early August and damages to rice during September. Based on these results, we suggest that the information on the rice leaf-folder population monitored by the adult density or damaged-hill percent in late July and early August would be very useful for predicting the damages later in the season for aiding in decision-making for timely control. In addition, the regional populations of rice leaf-folder showed the similar responses to the insecticides tested: high susceptibility to IGRs (tebufenozide and methoxyfenozide) and organophosphates (chlorpyrifos-methyl, pyridaphenthion), but relatively low to cartap.

Earthquake Monitoring : Future Strategy (지진관측 : 미래 발전 전략)

  • Chi, Heon-Cheol;Park, Jung-Ho;Kim, Geun-Young;Shin, Jin-Soo;Shin, In-Cheul;Lim, In-Seub;Jeong, Byung-Sun;Sheen, Dong-Hoon
    • Geophysics and Geophysical Exploration
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    • v.13 no.3
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    • pp.268-276
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    • 2010
  • Earthquake Hazard Mitigation Law was activated into force on March 2009. By the law, the obligation to monitor the effect of earthquake on the facilities was extended to many organizations such as gas company and local governments. Based on the estimation of National Emergency Management Agency (NEMA), the number of free-surface acceleration stations would be expanded to more than 400. The advent of internet protocol and the more simplified operation have allowed the quick and easy installation of seismic stations. In addition, the dynamic range of seismic instruments has been continuously improved enough to evaluate damage intensity and to alert alarm directly for earthquake hazard mitigation. For direct visualization of damage intensity and area, Real Time Intensity COlor Mapping (RTICOM) is explained in detail. RTICOM would be used to retrieve the essential information for damage evaluation, Peak Ground Acceleration (PGA). Destructive earthquake damage is usually due to surface waves which just follow S wave. The peak amplitude of surface wave would be pre-estimated from the amplitude and frequency content of first arrival P wave. Earthquake Early Warning (EEW) system is conventionally defined to estimate local magnitude from P wave. The status of EEW is reviewed and the application of EEW to Odesan earthquake is exampled with ShakeMap in order to make clear its appearance. In the sense of rapidity, the earthquake announcement of Korea Meteorological Agency (KMA) might be dramatically improved by the adaption of EEW. In order to realize hazard mitigation, EEW should be applied to the local crucial facilities such as nuclear power plants and fragile semi-conduct plant. The distributed EEW is introduced with the application example of Uljin earthquake. Not only Nation-wide but also locally distributed EEW applications, all relevant information is needed to be shared in real time. The plan of extension of Korea Integrated Seismic System (KISS) is briefly explained in order to future cooperation of data sharing and utilization.

Health Status and Associated Health Risks among Female Marriage Immigrants in Korea (한국 여성결혼이민자의 건강상태와 건강위험요인)

  • Kim, Hye-Kyeong;Yoo, Seung-Hyun;Cho, Seon;Kwon, Eun-Joo;Kim, Su-Young;Park, Ji-Youn
    • Korean Journal of Health Education and Promotion
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    • v.27 no.5
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    • pp.79-89
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    • 2010
  • Objectives: This study aims to identify health status and health risks among female marriage immigrants to Korea and to provide a basis for public health strategies to address their health issues. Methods: The participants of the study were 3,069 immigrant wives. The health examination was conducted by the Korea Association of Health Promotion (KAHP) in 2008. The participants also completed self-administered questionnaires on demographic characteristics, health-related behaviors and mental health. Results: Patterns of immigrant women's health problems differed by age and country of origin. Behavior patterns also differed by their heritage, age, and years of residence in Korea. Generally Vietnamese women fell in lower ranges of disease prevalence and health risk factors in the participant group and Japanese women presented most healthy eating habits. Filipina women showed relatively high disease prevalence than any other group. Conclusion: Immigration to Korea by marriage is relatively a new phenomenon, thus continuing surveillance and research are needed to identify health risks, behavior patterns, and their relationships. Interventions and policies for the health of migrant wives, their children and families are required.

Anisotrpic radar crosshole tomography and its applications (이방성 레이다 시추공 토모그래피와 그 응용)

  • Kim Jung-Ho;Cho Seong-Jun;Yi Myeong-Jong
    • 한국지구물리탐사학회:학술대회논문집
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    • 2005.09a
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    • pp.21-36
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    • 2005
  • Although the main geology of Korea consists of granite and gneiss, it Is not uncommon to encounter anisotropy Phenomena in crosshole radar tomography even when the basement is crystalline rock. To solve the anisotropy Problem, we have developed and continuously upgraded an anisotropic inversion algorithm assuming a heterogeneous elliptic anisotropy to reconstruct three kinds of tomograms: tomograms of maximum and minimum velocities, and of the direction of the symmetry axis. In this paper, we discuss the developed algorithm and introduce some case histories on the application of anisotropic radar tomography in Korea. The first two case histories were conducted for the construction of infrastructure, and their main objective was to locate cavities in limestone. The last two were performed In a granite and gneiss area. The anisotropy in the granite area was caused by fine fissures aligned in the same direction, while that in the gneiss and limestone area by the alignment of the constituent minerals. Through these case histories we showed that the anisotropic characteristic itself gives us additional important information for understanding the internal status of basement rock. In particular, the anisotropy ratio defined by the normalized difference between maximum and minimum velocities as well as the direction of maximum velocity are helpful to interpret the borehole radar tomogram.

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KOREAN MARS MISSION DESIGN USING KSLV-III (KSLV-III를 이용한 한국형 화성 탐사 임무의 설계)

  • Song, Young-Joo;Yoo, Sung-Moon;Park, Eun-Seo;Park, Sang-Young;Choi, Kyu-Hong;Yoon, Jae-Cheol;Yim, Jo-Ryeong;Choi, Joon-Min;Kim, Byung-Kyo
    • Journal of Astronomy and Space Sciences
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    • v.23 no.4
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    • pp.355-372
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    • 2006
  • Mission opportunities and trajectory characteristics for the future Korean Mars mission have designed and analyzed using KSIV-III(Korea Space Launch Vehicle-III). Korea's first space center, 'NARO space center' is selected as a launch site. For launch opportunities, year 2033 is investigated under considering the date of space center's completion with KSLV series development status. Optimal magnitude of various maneuvers, Trans Mars Injection (TMI) maneuver, Trajectory Correction Maneuver (TCM), Mars Orbit Insertion (MOI) maneuver and Orbit Trim Maneuver(OTM), which are required during the every Mars mission phases are computed with the formulation of nonlinear optimization problems using NPSOL software. Finally, mass budgets for upper stage (launcher for KSIV-III and spacecraft are derived using various optimized maneuver magnitudes. For results, daily launch window from NARO space center for successful Korean Mars mission is avaliable for next 27 minutes starting from Apr. 16. 2033. 12:17:26 (UTC). Maximum spacecraft gross mass which can delivered to Mars is about 206kg, with propellant mass of 109kg and structure mass of 97kg, when on board spacecraft thruster's Isp is assumed to have 290 sec. For upper stage, having structure ratio of 0.15 and Isp value of 280 sec, gross mass is about 1293kg with propellant mass of 1099kg and structure mass of 194kg. However, including 10% margins to computed optimal maneuver values, spacecraft gross mass is reduced to about 148kg with upper stage's mass of 1352kg. This work will give various insights, requiring performances to developing of KSIV-III and spacecraft design for future Korean Mars missions.

Narrative Inquiry : Practical experience of an Introduction to Engineering (공학입문 교과 실행경험에 관한 내러티브 탐구)

  • Park, Kyung-Moon;Kim, Taehoon
    • 대한공업교육학회지
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    • v.34 no.2
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    • pp.128-160
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    • 2009
  • Narratively I have described interactions between two teachers performing an introduction to the engineering class with various situations such as place, teacher, student and subject. I have specifically illuminated a three-dimensional narrative inquiry space embracing the culture of the university, the college of engineering and the ABEEK(Accreditation Board of Engineering Education of Korea)program. The result of the study is as follows: First, in order to stimulate the students' motivation, the teachers have to make not only their class PowerPoint slides match the size of the classroom, but the content of the slides must be condensed with core concepts. They also should utilized some video clips to empower students' interest in the subject within their classrooms. Second, the teachers should do various class activities in the classroom. Instead of spending most of the class time with his/her explanation, it would be advantageous for the teachers to allow the students to perform a task in class. Third, the teachers should ask their students about assignments which are helping students' understanding of the subject and planning of their future. Lastly, the teachers need to design the mid-term and the final tests inducing the students' motivation. Those tests also must test students' creativity and insight of the subject. Thus, the test should consist of an interpretive exercise and an essay type of item thus reducing the multiple choice types of items. There are several limitations to the study. First it is difficult to generalize what we found here because it is a case study. Second, we could not study in depth the effect of the interaction between the two teachers who were performing the introduction to the engineering course during the academic semester. Third, this study just probed into the difficulties of teaching the course. Hence, we have to understand more by focusing on each issue such as adapting to a new learning environment as a student from abroad, a practical experience boosting the students' interest in the introduction to the engineering course, also a practical experience on process based learning-versus result based learning, and an effective management of the student team presentation etc.

Effect of a 6-month Low Sodium Diet on the Salt Taste Perception and Pleasantness, Blood Pressure and the Urinary Sodium Excretion in Female College Students (6개월간의 저염식이 여자 대학생의 짠맛에 대한 인지와 기호, 혈압 및 뇨 나트륨 배설량에 미친 영향)

  • Chang, Soon-Ok
    • Journal of Nutrition and Health
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    • v.43 no.5
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    • pp.433-442
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    • 2010
  • The study aim was to examine the effect of sensory responses of subjects after 6-month dietary sodium reduction with the aid of nutritional education. Fourteen female college students voluntarily restricted their sodium intake for 6 months, during which time they received nutritional education on the low sodium diet. As a control group, 10 students, whose anthropometric measurement, sodium intake behavior, and blood pressure were not different from those of the experimental group, were maintained on a normal diet. For the sensory responses of subjects, the salt taste perception and pleasantness for graded (0.15-1.3%) NaCl solutions were measured by a 9-point hedonic scale. The optimum sodium concentration, urinary sodium excretion, and blood pressure were measured. All the measurements were done at the beginning and end of the experiment. The sensory evaluation revealed an absence of any difference between the two groups in salt taste perception and pleasantness responses at the beginning. After 6-month adaptation, the experimental group subjects showed higher responses to low NaCl solution (0.15, 0.3, 0.5%) in salt taste perception and pleasantness evaluation while the control group subjects exhibited the opposite response. The optimum sodium concentration was reduced from 105.6 mmol to 80.7 mmol (p = 0.015) and the urinary sodium excretion was also reduced from 1,398 mg to 906 mg (p = 0.041) only in the experimental group. Systolic blood pressure was significantly reduced in the experimental group, although there was no correlation between the urinary sodium excretion and blood pressure. The optimum sodium concentration was negatively correlated with the urinary sodium excretion (r = 0.418, p = 0.053), indicating that adaptation to low sodium diet can reduce sodium intake. Further study on the individual responses of subjects on a low sodium diet by periodical evaluation may provide useful data for setting the duration needed to stabilize a lowered appetite for sodium.