• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.6 no.4
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• pp.259-264
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• 2001

Typhoon and neap tide on Cochlodinium polykrikoides bloom and water temperature on disappearance of C. polykrikoides bloom were investigated to elucidate the outbreak mechanism of C. polykrikoides blooms at Naro and Namhae coastal area in South Sea of Korea. The first observation of C. polykrikoides blooms were observed when thermocline was disappeared by typhoon, tide, etc. The first blooms of C. polykrikoides were observed on neap tide or before one day from neap tide in 1996-1998 and 2000. However, thermocline was disappeared by typhoon in 1994 and 1999, the first blooms were observed early 12-30 day than 1996-1998 and 2000. The main reason of disappearance of C. polykrikoides blooms after typhoon on 1997-2000 seems to be other environmental change by typhoon rather than low water temperature. In the future, the first C. polykrikoides bloom will be appear around the first neap tide of latter part of August with breaking down of thermocline, but if the thermocline be collapsed by typhoon in July, the C. polykrikoides bloom will be appear at beginning of August. The outbreak of C. polykrikoides blooms will be explain as follows: The vegetative cells, which was germinated by environmental change or already exist in surface water at low level, input to the surface water, and then nutrients and trace metals which were suppled from out side of C. polykrikoides bloom area inflow to surface. The vegetative cells are growth by the nutrients and trace metals at suitable environmental conditions e.g. water temperature, salinity, and sufficient light.

• Korean Business Review
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• v.17
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• pp.53-81
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• 2004

The purpose of this study is to understand the realities and the subject of environmental management for small and medium-sized companies in Gwangwon area, through surveying the present status as to acquiring the certification of ISO14001, and to seek for a plan to facilitate environmental management. Given summarizing key results, those are as follows. First, while the number of companies in our country which acquired the certification of ISO14001, amounts to 1,215 businesses as of April of 2003, the number of small and medium-sized companies in Gwangwon area which obtained the certification of ISO14001 reached only 26 businesses, the lowest level among metropolitan municipalities. Second, for the reason that companies who didn't acquire the certification, strive not to receive the certification, it did present the point that' costs to be needed in acquiring and maintaining the certification are larger than practical benefit. Third, the biggest reason for either companies which did not acquire the certification of ISO14001 or companies which did (try to) acquire the certification of ISO1400, was, enhancement of a corporate image,' and the effect after a company who obtained the certification introduced the environmental management system, was also shown to be 'the improvement of a corporate image.' Fourth, many companies who acquired the certification of ISO1400 pointed out the response related to 'burden on document creation and costs' and 'lack of manpower' as problems when introducing the environmental management system. On the basis of major results of a study as the above, given presenting the subject and a plan for activating the environmental management of small and medium-sized companies in Gwangwon area, those are as follows. First, because most of companies who did not obtain the certification of ISO1400 have low recognition of ISO14001, it needs continuous and positive publicity, education and a training system. Second, it requires to carry out an educational program to nurture professional manpower due to lack of manpower relevant to environmental management, to expand payment of subsidies, to open exclusive-charge department and consulting contact, to have the relevant information be database and to develop software. Third, in order to make the certification obtained through inexpensive costs and simple procedures, it needs to positively consider the creation of public approval system for a small and medium-sized company, group approval system, industrial-complex approval system, and others.

• Journal of dental hygiene science
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• v.12 no.4
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• pp.413-421
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• 2012

The main purpose of this study is to present practical data for the development of the health enhancing programs for the dental hygiene students. This data is based on the investigation of the psychosomatic self-reported symptoms of the dental hygiene students. Out of the random selection of the current dental hygiene students in Gyeonggi-do and Gangwon-do districts. We conducted a survey and analyzed the collected data from 432 respondents. The results are as follows: 1. The mental appeals (19.37) were higher then the physical appeals (17.53) and of the items in psychosomatic symptoms, the point of mental instability the highest (21.85); whereas, that of mouth and anal scored the lowest (14.59). 2. In terms of the religion, statistical significance was shown among physical appeals (p<.01), mental appeals (p<.05), multiple subjective symptom (p<.01), digestive organs (p<.01), aggressiveness (p<.01), nervousness (p<.01), and eye and skin (p<.05), mental instability (p<.05). 3. Concerning the living conditions, Statistical significance was found on the items such as physical appeals (p<.05), mental appeals (p<.01), depression (p<.001), irregular and life (p<.001), multiple subjective symptom (p<.01), lie scale (p<.01) and mouth and anal (p<.05), digestive organs (p<.05). 4. As for regular health check-ups, statistical significance was shown in the following items such as mental appeals (p<.05), depression (p<.01), multiple subjective symptom (p<.05), mental instability (p<.05).

• Analytical Science and Technology
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• v.33 no.3
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• pp.151-158
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• 2020

Alcohol, which can easily be obtained in the same way as ordinary beverages, is harmful enough to cause death due to excessive drinking and chronic alcohol intake, so it is important to maintain a proper amount of drinking and healthy drinking habits. In addition, the incidence of behavioral disturbances and impaired judgments that can be caused by chronic alcohol drinking of more than adequate amounts of alcohol is also significant. Accordingly it is very useful for forensic science to check whether the person involved is drunken or is alcoholism state in various accidents. Currently, in Korea, alcohol consumption is determined by detecting the level of alcohol or alcohol metabolism 'ethyl glucuronide (EtG)' in blood or urine samples. However, analysis of alcohol or EtG in blood or urine can only provide information about the current state of alcohol consumption because of a narrow window of detection time. Therefore, it is important to analyze the EtG as a long-term direct alcohol metabolite bio-marker in human hair and to investigate relationship between alcohol consumption and EtG concentration for the evaluation of chronic ethanol consumption. In this study, we established an analytical method for the detection of EtG in Korean hair efficiently and validated selectivity, linearity, limits of detection (LOD), limits of quantification (LOQ), matrix effect, recovery, process efficiency, accuracy and precision using liquid chromatography tandem mass spectrometry (LC-MS/MS). In addition, the assay performance was evaluated in Korean social drinker's hair and the postmortem hair of a chronic alcoholism. The results of this study can be useful in monitoring the alcohol abuse of Korean in clinical cases and legal procedures related to custody and provide a useful tool to evaluate postmortem diagnosis of alcoholic ketoacidosis in forensics.

• The korean journal of orthodontics
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• v.28 no.1 s.66
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• pp.17-27
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• 1998

Study on growth change of dental arch is considered to both an important data in orthodontic diagonsis and treatment planning as well as analysis of treatment results , also, arch form is important in anthropology and dentistry, even more so in prosthodontics and orthodontics. In the field of orthodontics, studies on the functional aspect of upper and lower teeth and maintenance of stability of dentition and occlusion were carried out from the early days. Some of the early studies include explanation of growth change in dental arch from measuring directly fom human stroll, and afterwards, cephalometrics x-rays were introduced; accordingly, studies using cephalometric measurement and linear measurements of study models were often performed. By this method, arch width, arch depth and perimeters were measured, and growth change or dental arch was studied. The subject ror this study were sn children(boys and girls or ages from 3 yens to 12 years from Kang-won district and Seoul, who has no history of orthodontic treatment and who show healthy status and normal growth and development. Cephalometric x-ray, panoramic x-ray, and study model were taken for each subject consecutively for 2 years, and the subjects are still followed up. 400 pairs of study models from the past two years were used in this study; mesio-distal diameater of each tooth, intercanine width, intermolar width, canine depth, molar depth and arch perimeters were measured. Afterwards, mean value and each standard deviation of each age group and each gender were obtained, and representation graph were drawn. The following conclusion were obtained. 1. Intercanine width showed gradual increase until the age of 10-years and after that, showed no increase. 2. Intermolar width in upper arch showed gradual increase : intermolar width in lower arch showed no significant chang, and after the age of 9-years, showed increase. 3. Cainine arch depth showed relatively rapid increase after the age of 6-years, and this pattern was more obvious in lower arch. 4. Molar arch depth increased gradually in both archs and it decrease after the age of 10-years : this phenomenon was more prominent in the lower arch. 5. Arch perimeter showed gradual inerease and convert to plateau at the age of 10-years, after that, it decreased. this pattern was more prominent in lower arch.

• Journal of the korean academy of Pediatric Dentistry
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• v.26 no.2
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• pp.262-274
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• 1999

Several alternatives for increasing the fluoride concentration in the mouth, such as water fluoridation, ingestion of fluoride supplements, fluoride paste, fluoride mouthrinse, application of fluoride gel are available. There is an impressive body of evidence that the topically deliverd fluorides are clinically effective in inhibiting the progression of dental caries. Recent studies on the cariostatic action of fluoride have indicated the importance of fluoride in the fluid environment of the teeth. The fluoride levels in unstimulated whole saliva can be considered indicative of F in the aqueous phase available for interaction with the tooth surface at a given time. The retention of F in the mouth after topical fluoride treatment is considered to be an important factor in the clinical efficacy of F. The aim of this study was to determine the elevation and clearance of fluoride in whole saliv after the following topical flouride treatments using HMDS-diffusion technique and fluoride ion electrode. The obtained results were as follow: 1. Average salivary fluoride concentration in the unstimulated whole saliva was $0.0152ppm{\pm}0.0091ppm$. Unstimulated salivary flow rate was between 0.34-0.36ml/min and there was no statistically significant difference among the groups(p>0.05). 2. Except for the immediate time after treatment, fluoride levels followed as APF gel>neutral gel>F-rinse>F-paste. There was no statistical difference between the salivary F concentration of F-paste group and that of control group after 2 hours. In case of F-rinse group, after 3 hours the concentration had dropped to baseline value. But there was statistically significant difference among the F concentraion of F gel groups and that of control group(p<0.05). 3. The mean $AUC_{0-120min}$ values were followed as neutral gel>APF gel>F-rinse>F-paste, and the values of the two former groups were significantly higher than those of the two latter groups(p<0.05).

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.130-142
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• 2007

This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.

• Journal of Life Science
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• v.26 no.11
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• pp.1237-1244
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• 2016

A comprehensive analysis of the genetic diversity and relationship of the cold-water fishery walleye pollock (Theragra chalcogramma), the most abundant economically important fishery resource in the East sea of Korea, has not been carried out, despite its importance in Korea. The present study assessed the genetic diversity and relationship between five walleye pollock populations (Korean population, Russian population, USA population, and Japanese populations) of T. chalcogramma using eight microsatellite DNA (msDNA) markers to provide the scientific data for the preservation and management of the Pollock fishery resource. The results of the analysis of 186 individuals of the Pollock revealed a range of 7.13-10.63 numbers of alleles (mean number of alleles=9.05). The means of observed heterozygosity ($H_O$), expected heterozygosity ($H_E$) were 0.732 and 0.698, respectively. The results of genetic distance, Pairwise $F_{ST}$, UPGMA (UPGMA: un-weighted pair-group method with an arithmetical average) (the phylogenetic tree), PCA (PCA: Principal Coordinate analysis) analysis pointed to significant differences between the Korean population, Russian population, USA population, and Japanese populations, although small (p<0.05). These results shed light on the genetic diversity and relationships of T. chalcogramma and can be utilized for research on the evaluation and conservation of Korean T. chalcogramma as genetic resources.

• Journal of Life Science
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• v.21 no.5
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• pp.631-646
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• 2011

Mesenchymal stem cells (MSC) are multipotent and can be isolated from diverse human tissues including bone marrow, fat, placenta, dental pulp, synovium, tonsil, and the thymus. They function as regulators of tissue homeostasis. Because of their various advantages such as plasticity, easy isolation and manipulation, chemotaxis to cancer, and immune regulatory function, MSCs have been considered to be a potent cell source for regenerative medicine, cancer treatment and other cell based therapy such as GVHD. However, relating to its supportive feature for surrounding cell and tissue, it has been frequently reported that MSCs accelerate tumor growth by modulating cancer microenvironment through promoting angiogenesis, secreting growth factors, and suppressing anti-tumorigenic immune reaction. Thus, clinical application of MSCs has been limited. To understand the underlying mechanism which modulates MSCs to function as tumor supportive cells, we co-cultured human adipose tissue derived mesenchymal stem cells (ASC) with cancer cell lines H460 and U87MG. Then, expression data of ASCs co-cultured with cancer cells and cultured alone were obtained via microarray. Comparative expression analysis was carried out using DAVID (Database for Annotation, Visualization and Integrated Discovery) and PANTHER (Protein ANalysis THrough Evolutionary Relationships) in divers aspects including biological process, molecular function, cellular component, protein class, disease, tissue expression, and signal pathway. We found that cancer cells alter the expression profile of MSCs to cancer associated fibroblast like cells by modulating its energy metabolism, stemness, cell structure components, and paracrine effect in a variety of levels. These findings will improve the clinical efficacy and safety of MSCs based cell therapy.

• Journal of Aquaculture
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• v.19 no.1
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• pp.25-32
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• 2006

An experiment was carried out to investigate changing of water quality during the seed production of dark-banded rockfish Sebastes inermis in large scale tanks. Ten broodstock of dark-banded rockfish were held in three circular tanks (diameter 6.5 m; depth 2 m; water volume 50 ton) each (stocking density $0.061kg/m^3$). During the experiment the temperature ranged from 14.2 to $16.1^{\circ}C$. The fingerlings were 134 with rotifers only during 1 to 9 days after parturition, rotifers with Artemia nauplii during 10 to 20 days after parturition, Artemia nauplii only during 21 to 35 days after parturition, Artemia nauplii with commercial diet during 36 to 80 days after parturition and commercial diet only during 81 to 85 days after parturition. Water quality (dissolved oxygen, pH, $NH_4^+-N,\;NO_2^--N,\;NO_3^--N\;and\;PO_4^{3-}-P$) in rearing tanks measured every 5 days in long term monitoring investigation or every 2 hours in diurnal monitoring investigation. In 85 days after parturition, the body weight of fish grew up to 0.88 f and specific growth rate was 8.0%/day in body weight. In long term monitoring investigation, with the increase of the amount of supplied commercial diet, the concentration of dissolved oxygen (DO) and pH decreased, but the concentration of $NH_4^+-N\;(4.5\;to\;76.3{\mu}M),\;NO_2^--N\;(0.02\;to\;0.06{\mu}M),\;NO_3^--N\;(3.0\;to\;5.9{\mu}M)$, and $PO_4^{3-}-P\;(0.41\;to\;0.59{\mu}M)$ increased. In the diurnal monitoring investigation, the concentration of $NH_4^+-N$ showed great fluctuation and ranged from 3.0 to $9.1{\mu}M$ when fed rotifers, 16.3 to $45.8{\mu}M$ when fed Artemia nauplii and 36.5 to $120.1{\mu}M$ when fed commercial diet. After daily feeding with each of feed, the amount of dissolved inorganic nitrogen (DIN) and phosphorus (P) wastage were 7.0 g and 0.7 g when fed rotifers, 24.7 g and 0.7 g when fed Artemia nauplii and 140.9 g and 2.2 g when 134 commercial diet. The amount of DIN and phosphorous wastage during 134 commercial diet was significantly higher than that of fed rotifer and Artemia nauplii (P<0.05). Results will provide valuable information far water quality management and culture of dark-banded rockfish in commercial seed production systems.