• Korean Journal of Environment and Ecology
• /
• v.12 no.1
• /
• pp.58-77
• /
• 1998

Four species(25%) of Viperidae(Agkistrodon brevicaudus, Agkistrodon ussuriensis, Agkistrodon saxatilis) and Cloubridae(Rhabdophis tigrenus tigrenus) were Korean poisonous snake. Copulation season of these species was from July to August. Reproduction mode of genus Agkistrodon species was ovoviviparous but Rhabdophis tigrinus tigrinus was the other pattern of oviparous. Optimal movement temperature range was from 20$\circ $C to 29$\circ $C(March~September). Wjen atmosphere temperature was below 10$\circ $C, at that time they hibernate at the ground, rock bottom, stone wall and embankment around the end of a field. The venom of these snakes consist mainly Hematoxin, Cytolysin, Neurotoxin and Cardiotoxin of poisonous liquids. These material injection to animal cause systemic syndrome such as Dizziness(25.7%), Vomitting(23.1%), Fever(22%), Visual trouble(18%), Headace(17.7%), Dyspnoea(17.6%) and bring about other local syndrome such as Discoloration(54.2%), Bleeding(20.2%), Bullae(10.7%) and Skin ulcer(!0.8%). The annual distribution was appeared to decrease 1972 after 1992 and average snakebiting patients was 25.6 per year, but practically total estimated snakebiting was 2,700 per year. The seasonal distribution was most frequent in August(25%), and mortality was 1.8%(26 per 1,430). The sex ratio was 2:1 and according to age distribution, it was most prevalent at one's fifties(19%). The most frequent place where the accident happened was the field(48.2%) and most predilection site of the body for victim were hand(47.8%) and foot(39.5%), Commonly bite snake were Agkistrodon ussuriensis(27.1%), Agkistrodon brevicaudus(22.6%) and Agkistrodon saxatilis(9.6%) but 40.7% of species could not be identified. Treatment of antivenin patient was 75.9% (1,068/1,407).

• The Korean Journal of Pharmacology
• /
• v.16 no.1 s.26
• /
• pp.25-34
• /
• 1980

The in vitro guinea pig ureter responded to 5 sec trains of electrical stimuli with two contractions; the first an 'on response' (ON) occurred with $0.1{\sim}0.3$ sec after the onset o the stimulus train, the second an 'off response'(OFF) occurred $0.2{\sim}1.0$ sec after the termination of the stimulus train. Relaxation occurred between the two responses during a time when the stimulus was still being delivered. Longer duration and/or higher frequencies of stimuli within the train were required to elicit the OFF than the ON. Decreasing temperature from $37^{\circ}$ to $22^{\circ}$ decreased ON amplitude and increased OFF amplitude. $Ca^{++}$-free solution, 2 mM EDTA, 1 mM $Mn^{++}$ or $1{\mu}M$ verapamil rapidly abolished ON. OFF persisted when ON had disappeared by repeated stimulation at 0.12 train per sec. Conversely, caffeine, $50{\mu}M$ and theophylline, $10{\mu}M$ abolished OFF with only slight reduction of ON, and sodium nitroprusside decreased preferentially ON amplitude rather than OFF. Relaxation between ON and OFF was incomplete in low $Na^+$ solution. ON and OFF were not affected by the neural blockers tetrodotoxin, atropine or phentolamine, also pyrilamine and methysergide, and relaxation between ON and OFF was $Na^+$ dependent. Furthermore, ON depends on free $Ca^{++}$ and OFF is more dependent on bound or stored $Ca^{++}$.

• Korean Journal of Plant Resources
• /
• v.36 no.2
• /
• pp.107-121
• /
• 2023

This study analyzed the polyphenol, flavonoid contents, antioxidant activity, anti-aging activity and phenol component contents of Saccharina japonica (SJ), Costaria costata (CC) extracts with hot water, 95% methanol, 95% prethanol for investigating possible utilization of SJ and CC extracts. The result revealed that the SJ and CC methanol extracts showed the highest polyphenol and flavonoid contents, 4.63 mg TAN/g, and 4.19 mg QUE/g respectively. Also, the SJ and CC methanol extracts showed higher antioxidant activity than prethanol and hot water extracts, whereas the ABTS radical scavenging activities were the highest in prethanol extracts (IC₅₀ = 15.4, 10.3 ㎍/µL). In anti-aging activity for evaluating the anti-wrinkle activity and skin whitening activity, the CC methanol extracts had high collagenase inhibitory activity (88.3%), and the SJ prethanol extracts showed higher elastase inhibitory activity (19.0%) compared to other extracts. Then the tyrosinase inhibitory activity was significantly higher in the SJ and CC methanol extracts (41.8, 30.3%, respectively), whereas prethanol extracts were the lowest. To identify the phenol component contents of SJ and CC extracts, 4-hydroxybenzoic acid, naringenin, naringin and nicotinic acid were measured using LC-MS/MS. As a result, the phenol contents were the highest in SJ methanol extract (4-hydroxybenzoic acid), SJ and CC prethanol extract (naringin and naringenin) and CC prethanol extract (nicotinic acid). Lastly, the antioxidant activity of SJ and CC showed high correlations with polyphenol and flavonoid contents (R = -0.946~0.883). These results suggest that prethanol or methanol extracts of SJ and CC have higher antioxidant activities, anti-aging activity and the potential to be used as material for health functional food and cosmetics.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.222-233
• /
• 2022

The heading responses of rice varieties that originated from South Korea, North Korea, and northern China were examined under the temperature and day-length conditions of 13 major rice production areas in North Korea. Kenjiandao3 and Nongdae3 originated from China, Olbyeo1, Olbyeo2 and Sonbong9 from North Korea, and Joun from South Korea demonstrated the earliest heading stage depending on the regional environment. Out of 40 rice varieties, 34 reached the heading stage within the regional safe marginal heading date (SMHD) under Haeju and Sariwon environmental conditions, while 16 to 17 varieties reached the heading stage under Wonsan, Changjon, Supung, and Yongyon environmental conditions. Some middle and mid-late maturing varieties that originated from South Korea reached the heading stage within the SMHD under the temperature and day-length conditions of Kaesong, Haeju, Sariwon, Nampo, and Pyongyang that are located in the west-southern plain. The majority of early maturing varieties, but not the middle or mid-late ones, reached the heading stage within the SMHD under the environmental conditions of Singye, Anju, Kusong, and Sinuiju. Only a few early maturing varieties demonstrated the heading stage within the SMHD under Yongyon, Changjon, and Wonsan environments. The number of days to heading was highly positively correlated among all regions; however, it was not consistent among the rice varieties. The 40 rice varieties that had been tested were classified into seven groups according to their heading responses to the temperature and day-length variations of the 13 regional conditions at 65% similarity level in cluster analysis.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.234-241
• /
• 2022

Wheat (Triticum aestivum L.) is a major staple foods and is in increasing demand in the world. The elevated temperature caused by changes in climate and environmental conditions is a major factor affecting wheat development and grain quality. The optimal temperature range for winter wheat is between 15 and 25℃, and it is necessary to study the physiological characteristic of wheat according to elevated temperatures. This study presents the effect of elevated temperature on the yield and quality of two Korean bread wheat (Baekkang and Jokyoung) in temperature gradient tunnels (TGT). Two bread wheat cultivars were grown in TGT at four different temperature conditions: T0 (control, near ambient temperature), T1 (T0+1℃), T2 (T0+2℃), (T0+2℃), T3 (T0+3℃). The period from sowing to heading stage accelerated and the number of grains per spike and grain yield reduced under T3 condition compared with those under T0 condition. Grain filling rate and grain maturity also accelerated with elevated temperature (T3). The increase in temperature led to the increase in protein contents, whereas decreased the total starch contents. These results are consistent with the decreased expression of starch synthesis genes and increased gliadin synthesis or gluten metabolism genes during the late grain filling stage. Taken together, our results suggest that the increase in temperature (T3) led to the decrease in grain yield by regulating the number of grains/spike, whereas increased the protein content by regulating the expression of starch and gliadin-related genes or gluten metabolism process genes expression. In addition, our results provide a useful physiological information on the response of wheat to heat stress.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.342-361
• /
• 2022

In order to evaluate the effect of nitrogen application levels on yield and quality of rice varieties, a field experiment was conducted at National Institute of Crop Science of Korea from 2018 to 2020. Five levels (0, 3, 5, 7, and 9 kg/10a) of nitrogen fertilizer were treated to 21 Korean rice varieties. Yield, yield component, appearance quality, and protein content in rice were analyzed. The average head rice yield for 3 years decreased by 28%, 22%, 11%, and 8%, respectively, when cultivated with 0, 3, 5, and 7 kg/10a nitrogen application compared to cultivation with a standard nitrogen application amount, 9 kg/10a. The number of panicles per hill increased as the amount of nitrogen application increased, but there was no significant change in the number of grains per panicle and 1000-grains weight, and the number of panicles per hill showed relatively small annual variation compared to other yield components. There was no significant difference in the head rice ratio according to the nitrogen application amount, the broken rice ratio slightly decreased, and the floury rice ratio increased. The protein content of rice decreased with increasing nitrogen application in 2018 and 2019, and was the lowest at 7 kg/10a of nitrogen application, and showed a tendency to increase again at 9 kg/10a. In the case of 2020, as the amount of nitrogen application increased, the protein content showed a tendency to continuously increase. In terms of varieties, 13 varieties, including Chilbo, seemed to be capable of low-nitrogen cultivation because loss of the head rice yield was less and the protein content could be lowered to 6% or less according to 7 kg/10a nitrogen application.

• Development and Reproduction
• /
• v.12 no.3
• /
• pp.289-296
• /
• 2008

BPES (Blepharophimosis/Ptosis/Epicanthus inversus Syndrome) is an autosomal dominant disorder caused by mutations in FOXL2. Affected individuals have premature ovarian failure (POF) in addition to small palpebral fissures, drooping eyelids, and broad nasal bridge. FOXL2 is a member of the forkhead family transcription factors. In FOXL2-deficient ovaries, granulosa cell differentiation dose not progress, leading to arrest of folliculogenesis and oocytes atresia. Using yeast two-hybrid screening of rat ovarian cDNA library with FOXL2 as bait, we found that small ubiquitin-related modifier (SUMO)-conjugating E2 enzyme UBE2I protein interacted with FOXL2 protein. UBE2I also known as UBC9 is an essential protein for processing SUMO modification. Sumoylation is a form of post-translational modification involved in diverse signaling pathways including the regulation of transcriptional activities of many transcriptional factors. In the present study, we confirmed the protein-protein interaction between FOXL2 and UBE2I in human cells, 293T, by in vivo immunoprecipitation. In addition, we generated truncated FOXL2 mutants and identified the region of FOXL2 required for its association with UBE2I using yeast-two hybrid system. Therefore, the identification of UBE2I as an interacting protein of FOXL2 further suggests a presence of novel regulatory mechanism of FOXL2 by sumoylation.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.1
• /
• pp.1-8
• /
• 1992

This study was conducted to obtain the basic information on salt-tolerance of rice cultivars in relations with growth retardation and cation content. As the salt-level increased, less retardation in plant height and dry weight were shown in salt-tolerant than salt-susceptible rice cultivars. Salt tolerant cultivars showed lower N $a^{+}$ and higher $K^{+}$ content and lower N $a^{+}$ / $K^{+}$ ratio at each salt-levels than salt-susceptible ones, while there were no significant differences in $Ca^{++}$ and $Mg^{++}$ content. At the tillering stage, the plant height and dry weight of the salt-treated plots were significantly correlated with N $a^{+}$ and $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio, implying that N $a^{+}$ and $K^{+}$ content could be an indicator of salt-tolerance of a rice cultivar. There were no tiller-depending differences in cation content in all cultivars. N $a^{+}$ content and N $a^{+}$ / $K^{+}$ ratio in leaves were lower at the top and higher at the bottom. In three $F_1$ hybrids between salt tolerant parent Pokkali and three salt susceptible parents, plant height, dry weight, $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio were similar to those of Pokkali parent, while N $a^{+}$ content was intermediate of the parents. So, it seemed that salt tolerance is dominant over salt susceptibility. In $F_2$ of Pokkali/wx817 cross, genetic segregation of plant height, dry weight, $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio varied continuously and was biased onto Pokkali side, while that of N $a^{+}$ content showed normal distribution with intermediate mode. Broad-sense heritability of the characters ranged from 0. 604 to 0. 811. Genotypic and phenotypic correlation coefficients among them were relatively high.fficients among them were relatively high.h.h.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.14
• /
• pp.159-164
• /
• 1973

This study was carried out in the central part of Korea for three years from 1970 to 1972. Its aim was to investigate the variations of growth and yield of rice varieties of different cultivating methods (transplanting culture, direct sowing culture on irrigated paddy field, and direct sowing culture on non-irrigated paddy field. The results could be summarized as follows; 1. The number of days from sowing to heading by direct sowing culture on irrigated paddy field was remarkably shorter than transplanting culture. Direct sowing culture on non-irrigated paddy field was longer than transplanting culture. 2. The number of panicles showed direct sowing culture on irrigated paddy field was first, direct sowing culture on non-irrigated paddy field was second, and third transplanting culture. But the number of spikelets per panicle was first on the transplanting culture, second on the direct sowing culture on non-irrigated paddy field and third on the direct sowing culture on irrigated paddy field. 3. The weight of the brown rice showed that direct sowing culture on irrigated paddy field produced the highest yield among the cultivating method in all varieties. The transplanting culture increased more than the direct sowing culture on non-irrigated paddy field. The highly productive varieties were Sadominory, Akibale, Suwon 213-1 when the direct sowing culture on irrigated paddy field was used. 4. The correlation between the brown rice yield and the yield components were as follows; in each cultivating method, it showed highly significant positive correlation between the brown rice yield and the 1, 000 grain weight of hulled rice, and between the brown rice yield and the number of the spiklets per panicle in the transplanting culture, in the direct sowing culture On irrigated paddy field between the brown rice yield and the number of spikelets per panicle and between the brown rice yield and the number of panicles, in direct sowing culture on non-irrigated paddy field between the brown rice yield and number of panicles. 5. It showed highly significant negative correlation between the brown rice yield and the number of days from sowing to heading in the direct sowing culture on irrigated and non-irrigated paddy field, but it was showed very low negative correlation in the transplant culture.

• Korean Journal of Plant Resources
• /
• v.36 no.1
• /
• pp.15-25
• /
• 2023

Myriophyllum spicatum L. has been used as an ornamental in ponds and aquariums, and as a folk remedy for inflammation and pus. Nevertheless, the biological activity and underlying mechanisms of anti-inflammatory effects are unclear. This study is aimed at investigating the antioxidative and anti-inflammatory activities of ethanol extract of Myriophyllum spicatum L. (EMS) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Antioxidant activity of EMS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. As inflammatory response parameters produced by LPS-stimulated RAW 264.7 cells were quantified to assess the anti-inflammatory activity of EMS. Our results showed that EMS increased FRAP and DPPH radical-scavenging activity. In EMS-treated RAW 264.7 cells, the production of NO, PGE₂, TNF-α and IL-1β was significantly inhibited at the non-cytotoxic concentration. In addition, EMS significantly attenuated LPS-stimulated the toll-like receptor (TLR) 4/myeloid differentiation protein (MyD) 88 signaling pathway, and inhibited nuclear translocation of nuclear factor-kappa B(NF-κB). Positive correlations were noted between anti-inflammatory activity and antioxidant activity. In conclusion, it was indicated that EMS suppresses the transcription of inflammatory factors by inhibiting the TLR4/MyD88/NF-κB signaling pathway, thereby suppressing LPS-stimulated inflammation in RAW 264.7 cells. This study highlights the potential role of EMS against inflammation and associated diseases.