• Title/Summary/Keyword: 접힘 현상

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Eshcherichia coli 로부터 inclusion body 형태로 발현한 LK68의 solid-phase refolding 및 정제

  • Choe, Won-Chan;Seo, Chang-U;Ryu, Gang;Jeong, Gyeong-Hwan;Lee, Eun-Gyu
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.485-488
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    • 2002
  • 재접힘 고체상 재접힘은 높은 재현성을 보였으며 고체상 재접힘된 단백질은 Native와 같은 구조를 형성하였다. 따라서 이 연구는 고체상 재접힘 방법이 분자간의 상호작용을 억제하는 것이 응집현상을 탈피하게된 결과 일 것이라는 것에 의해 재접힘 수율을 높일 수 있다고 기대한다.

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Solid-Phase Refolding of Poly-Lysine fusion Protein of hEGF and Angiogenin (Poly-lysine이 연결된 hEGF와 angiogenin의 융합단백질의 고체상 재접힘)

  • Park, Sang-Joong;Ryu, Kang;Suh, Chang-Woo;Chai, Young-Gyu;Kwon, Oh-Byung;Park, Seung-Kook;Lee, Eun-Kyu
    • KSBB Journal
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    • v.17 no.2
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    • pp.153-157
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    • 2002
  • A fusion protein, consisting of a human epidermal growth factor as the recognition domain and human angiogenin as the toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as an inclusion body in recombinant E. coli, yet when the conventional solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably because of the opposite surface charge resulting from the vastly different pl values of each domain. Accordingly the solid-phase refolding process, which exploits the ionic interactions between a solid matrix and the protein, was tried, however the ionic binding yield was also very low regardless of the resins and pH conditions used. Therefore, to provide a higher affinity toward the solid matrix, six Iysine residues were tagged to the N-terminus of the hEGF domain. When cation exchange resins, such as heparin- or CM-Sepharose, were used as the matrix, the adsorption capacity increased 2.5~3-fold and the subsequent refolding yield increased nearly 15-fold compared to the conventional process. A similat result was also obtained when an Ni-NTA metal affinity resin was used.

In Vitro Refolding of Inclusion Body Proteins Directly from E. coli Cell Homogenate in Expanded Bed Adsorption Chromatography (Expanded Bed Adsorption 크로마토그래피를 사용하여 재조합 E. coli 세포 파쇄액으로부터 내포체 단백질을 직접 재접힘하는 공정)

  • 조태훈;서창우;이은규
    • KSBB Journal
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    • v.16 no.2
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    • pp.146-152
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    • 2001
  • To avoid the intrinsic problem of aggregation associated with the traditional solution-phase refolding process, we propose a solid-phase refolding method integrated with expanded bed adsorption chromatography. The model protein used was a fusion protein of recombinant human growth hormone and a glutathione S transferase fragment. It was demonstrated that the EBA-mediated refolding technique could simultaneously remove cellular debris and directly renature the fusion protein inclusion bodies in the cell homogenate with much higher yields and less agregation. To demonstrate the applicability of the method, we successfully tested the three representative types of starting materials, i. e., rhGH monomer, washed inclusion bodies, and the E. coli homogenate. This direct and simplified refolding process could also reduce the number of renaturation steps required and allow refolding at a higher concentration, at approximately 2 mg fusion protein per ml of resin. To the best of our knowledge, it is the first approach that has combined the solid-phase refolding method with expanded bed chromatography.

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Characteristics and mechanisms of Tropospheric Ozone Enhancement in Springtime (봄철 대류권오존의 고농도특성과 발생원인에 관한 연구)

  • 김유근;송상근;오인보
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 2001.11a
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    • pp.75-76
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    • 2001
  • 대류권오존의 기원은 크게 두 부분으로 나누어진다. 첫 번째, 오존의 전구물질인 CO, NOx, 그리고 non-methan hydrocarbon이 빛과 작용하여 형성되어지는데, 이러한 조건에 부합되는 시기는 태양의 일사량이 풍부하고 온도가 높은 5∼9월경이다. 두 번째는, 제트기류가 위치하는 곳에서 대기의 섭동에 의해 대류권계면 접힘 (tropopause folding) 현상 발생시 오존 전량의 90%가 존재하는 성층권에서 다량의 오존이 대류권으로 유입되기도 한다 (Fishman et al., 1979; Uccellini et al., 1985). (중략)

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Development of Unfolding Radial Velocity Algorithm for Dual PRF Mode of Yong-In Testbed(YIT) Radar (용인테스트베드레이다를 이용한 Dual PRF 모드의 시선속도 접힘 풀기 알고리즘 개발)

  • Kim, Hye-Ri;Suk, Mi-Kyung;Nam, Kyung-Yeub;Ko, Jeong-Seok
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.27 no.6
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    • pp.521-530
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    • 2016
  • Weather radar is observation equipment that transmits electromagnetic waves and receives backscattered signals from the targets. The weather radar systems of the Korea Meteorological Administration have a doppler mode that can extract the target's radial velocity. However, the radial velocity over the maximum unambiguous velocity(${\nu}_m$) for which is in a trade-off relationship with the maximum unambiguous range is folded. Therefore, a dual PRF mode of which transmits and receives signals using two different PRFs(high and low) must be used to extend the vm while maintaining the maximum unambiguous range. Using a dual PRF mode, vm can be extended to the amount of lowest common denominator of two observed vm from high and low PRF. For this extension, we have developed a velocity unfolding algorithm of which uses several criteria for classification considering observed velocity differences between high and low PRF and their error boundary. Then, correction factors are calculated for each class and are applied to unfold radial velocity. The developed algorithm was applied to the Yong-In Testbed(YIT) radar and the generated better performance of radial velocity extraction than those of the previous system.

Simulations of Self-Assembled Structures in Macromolecular Systems: from Atomistic Model to Mesoscopic Model (고분자 자기조립 구조의 전산 모사: 원자 모델로부터 메조 스케일 모델까지)

  • Huh, June;Jo, Won-Ho
    • Polymer(Korea)
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    • v.30 no.6
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    • pp.453-463
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    • 2006
  • Molecular simulation is an exceptionally useful method for predicting self-assembled structures in various macromolecular systems, enlightening the origins of many interesting molecular events such as protein folding, polymer micellization, and ordering of molten block copolymer. The length scales of those events ranges widely from sub-nanometer scale to micron-scale or to even larger, which is the main obstacle to simulate all the events in an ab initio principle. In order to detour this major obstacle in the molecular simulation approach, a molecular model can be rebuilt by sacrificing some unimportant molecular details, based on two different perspectives with respect to the resolution of model. These two perspectives are generally referred to as 'atomistic' and 'mesoscopit'. This paper reviews various simulation methods for macromolecular self-assembly in both atomistic and mesoscopic perspectives.

A Technique for Abstract Representation of Protein Tertiary Structure (단백질 3차 구조의 추상적인 표현기법)

  • 김진홍;안건태;변경익;윤형석;이수현;이명준
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.10a
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    • pp.595-597
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    • 2001
  • 오늘날 인간 유전체 프로젝트(Human Genome Project)의 완성은 인간의 모든 유전자 서열정보를 제공하게 되었으며, 이러한 데이터를 바탕으로 생명현상과 관련된 산업 및 연구가 각광받게 되었다. 특히 생명체의 특정 기능을 파악하기 위한 단백질 3차 구조에 대한 연구가 활발히 진행중이다. 본 논문에서는 단백질 3차 구조를 추상적으로 기술 할 수 있는 표현기법을 기술한다. 제안된 표현기법은 단백질 2차 구조요소($\alpha$-나선구조와 $\beta$-병풍구조)를 이용하여 인접한 구성요소간의 접힘(folding)에 대한 관계를 기술하여 추상적인 단백질 3차 구조를 표현한다. 제안된 표현기법으로 기술된 추상적 단백질 3차 구조 표현은 단백질 구조에 대한 보다 빠른 이해와 다른 단백질 구조와 비교될 수 있는 장점을 지닌다.

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A Study on Real Time Control of Resin Transfer Molding (RTM 공정의 실시간 제어에 관한 연구)

  • Jeon Young Jae;Um Moon Kwang;Byun Joon Hyung;Lee Woo Il
    • Composites Research
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    • v.18 no.4
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    • pp.35-43
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    • 2005
  • In resin transfer molding(RTM), race-track effects and non-uniform fiber volume fraction may cause undesirable resin flow patterns and thus result in dry spots, which affect the mechanical properties of the finished parts. In this study, a real time RTM control strategy to reduce these unfavorable effects is proposed. This control rule is accomplished by means of the permeability mapping and pressure regulation. Through numerical simulations, the validity of the proposed scheme is demonstrated.

Development of a general purpose molecular simulation system from microscopic to mesoscopic scales (미시영역에서 중간역역까지 적용 가능한 범용 분자 시뮬레이션 시스템의 개발)

  • Oh, Kwang-Jin
    • The KIPS Transactions:PartD
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    • v.12D no.6 s.102
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    • pp.921-930
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    • 2005
  • In this paper, a general purpose molecular simulation system which has been developed by the author, are described. One of the most advantageous features is that the molecular simulation system can handle a coarse-grained model as well as an all-atom mode. Therefore, we can simulate mesoscopic phenomena as well as microscopic phenomena with the help of Langevin dynamics simulation and dissipative particle dynamics simulation techniques. Thus we could study anesthesia, protein folding, biopolymer flow in microchannel with single framework, which spans from microscopic to mesoscopic scales. We expect that we can also simulate many other bio/nano systems of technological importance which are not feasible by means of molecular dynamics simulation technique. Finally, performance data are shown and a bottleneck is identified for future optimization.