• Journal of Life Science
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• v.29 no.10
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• pp.1096-1103
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• 2019

Neuroinflammation is mediated by the activation of microglia and has been implicated in the pathogenesis of neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease. Therefore, the inhibition of neuroinflammation may be an effective solution to treat these brain disorders. Protaetia brevitarsis seulensis is an insect belonging to the order Coleoptera and inhabits Korea, China, Japan and Siberia. P. brevitarsis seulensis is an edible insect that can be consumed as a protein source for humans. It has been reported that P. brevitarsis seulensis contains useful bioactive substances for hepatoprotection and improving blood circulation, such as indole alkaloids. Microglia cells are the main source of proinflammatory cytokines and nitric oxide (NO) in the central nervous system, which Perform neuroimmune, inflammatory, and other neurobilogical functions. In this study, we investigated the anti-neuroinflammatory effects of P. brevitarsis seulensis ethanol extract (PBE) in activated microglia cells treated with lipopolysaccgarude (LPS, 100 ng/ml). As a result, PBE significantly inhibited NO production without cytotoxicity and decreased the expression levels of inducible NO synthase and cyclooxygenase-2. In addition, the production of inflammatory cytokine secreted by LPS was also reduced by PBE. These results suggest that PBE could be a good source of functional substances to prevent neuroinflammation and neurodegenerative diseases.

• Proceedings of the Korean Society of Computer Information Conference
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• 2020.07a
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• pp.575-576
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• 2020

본 논문은 근래에 식용곤충 식품에 대한 수요 및 국민적 관심이 증가하여 관련 산업이 급격히 성장하고 있는 가운데, 건강기능성 효과가 널리 알려진 흰점박이꽃무지 유충의 안정적인 생산량 확보를 위한 스마트 사육사를 제작하고 그 성능을 평가한 결과이다. 사육사는 L6m×W3m×H2.8m 크기로 제작하였으며, 안정적인 사육환경을 위하여 사육실과 공조실을 분리하여 설계하였다. 공시재료는 생후 15일이 경과된 흰점박이꽃 무지 유충 1령이며, 스마트 사육사 내 사육환경은 온도 25±2℃, 습도 65±5%로 제어하였다. 사육조사는 매주 1회, 유충의 체중, 길이, 두께를 측정하였으며, 스마트 사육사의 성능평가를 위해 일반 사육농가(전북 소재)와 비교·분석하였다. 사육 4주 후 조사 결과, 스마트 사육사에서 사육한 유충의 체중과 길이는 각각 평균 1.97g/마리와 3.75cm로, 일반농가의 1.58g/마리와 3.55cm에 비해 비교적 높은 것으로 나타났다. 하지만, 두께의 경우 2주 차까지 일반농가에서 대체로 높은 것으로 나타났으며, 이후 3~4주 차에서는 큰 차이를 보이지 않았다. 따라서 본 연구를 통해 개발한 흰점박이꽃무지 유충 스마트 사육사는 일반농가와 비교해 사육이 비교적 빠르고 생산량을 더 많이 확보할 수 있는 시스템으로 농가소득 증대에 유용할 것으로 판단되며, 장소 및 시간에 상관없이 생육환경 제어가 가능하여 개발된 시제품의 보급 확대가 필요하다.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2000.10a
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• pp.20-20
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• 2000

• Proceedings of the Korean Society of Computer Information Conference
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• 2020.07a
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• pp.577-578
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• 2020

본 연구의 목적은 흰점박이꽃무지 사육농가 및 사육환경에 대해 일반농가의 실태를 조사·분석하여 식용곤충 사육시설에 대한 기초 연구자료를 수집하기 위한 것이다. 전국에 곤충사육 농가로 등록한 업체 중에서 흰점박이꽃무지를 사육하는 농가 17농가를 대상으로 설문조사를 실시하여 농장운영 및 시설·장치 현황 등을 조사하였으며, 주요 결과는 다음과 같다. 일반적으로 곤충사육 농가는 대부분 농가형으로 운영되고 있지만, 생산업, 유통업 및 가공업 등으로 신고하여 곤충사육 농가에서 직접 생산, 가공 및 제품 개발 등을 하고 있는 것으로 나타났다. 그리고 대부분의 곤충사육 농가는 판넬 형식의 건축물 내에서 냉·난방기를 가동하여 곤충의 생육환경을 조성하고 있으며, 필요에 따라 IoT 기반의 사양관리 장치 및 운영관리 시스템을 활용하고 있는 것으로 파악되었는데, 식용곤충 대량생산을 위한 사양관리 장치 및 생산 기반시설 구축은 여전히 부족한 상황으로 개선이 필요해 보인다.

• Journal of Life Science
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• v.31 no.5
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• pp.475-480
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• 2021

Interest in edible insects such as Protaetia brevitarsis has increased rapidly, and several insect producers use these insects in industrialized mass production. However, mass rearing of insects can cause insect diseases. Sprouted barley is a valuable source of nutrients and has antioxidant, antimicrobial, anti-inflammatory, and anti-cancer effects. This study was conducted to investigate the effect of sprouted barley as a feed additive for producing healthy P. brevitarsis larvae. P. brevitarsis larvae were fed feeds with or without sprouted barley, and their body weight and larval period wewe checked weekly. To confirm the antibacterial effects of sprouted barley, in vitro bioassays were performed by counting Serratia marcescens colonies, and in vivo bioassays were performed by determining the survival rate and body weights of the S. marcescens-infected larvae. Larvae fed different feeds were analyzed for their nutrient compositions (i.e., such as proximate composition, minerals, amino acids, and heavy metals). Larvae fed 5% and 10% sprouted barley had maximum weight increases of 19.2% and 23.1%, respectively. Both treatment groups had significantly shorter larval periods than those of the control group. Sprouted barley markedly inhibited the growth of entomopathogenic S. marcescens. Furthermore, larvae fed sprouted barley exhibited higher Cu, Zn, and K levels. Seventeen amino acids were present in larvae fed sprouted barley, of which, tyrosine and glutamic acid were predominant. No heavy metals were detected in any of the investigated groups. Therefore, sprouted barley may be a suitable feed additive for producing high-quality P. brevitarsis larvae.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.447-454
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• 2023

In the environment in which humans live, there are various antigens that invade the human body and interfere with humans leading a healthy life, so the immune system recognizes the antigen then removes them through a complex mechanism. Macrophages are widely distributed immune cells involved in the innate immune system, and produce various immune modulators such as inducible nitric oxide synthase-induced nitric oxide, cyclooxygenase-2 induced prostaglandin E2 and proinflammatory cytokines such as tumor necrosis factor-alpha. On the other hand, Protaetia brevitarsis seulensis larvae are a type of edible insect that have emerged as an alternative to the future food supply problem. The immuno-modulatory effect through the activation of murine macrophage RAW264.7 cell via mitogen-activated protein kinases (MAPKs)/nuclear factor-kappa B (NF-κB) signaling pathways has been reported. Based on this report, in this study, we confirmed how the expression of immune modulators induced by Protaetia brevitarsis seulensis larvae extracts in RAW264.7 cells was changed by treatment with pharmacological inhibitors of toll-like receptor 4 (TLR4), MAPKs and NF-κB signaling pathways. As a result, reduction of immune modulators was confirmed in the c-Jun N-terminal kinase (JNK) inhibitor treatment group and NF-κB inhibitor treatment group among the Protaetia brevitarsis seulensis larvae-treated RAW264.7 cell. Furthermore, in the TLR4 inhibitor-treated group, decreases in phosphorylation of JNK and NF-κB factors were confirmed in Protaetia brevitarsis seulensis larvae-treated RAW264.7 cell, as well as decreases in immune modulators. This results suggest that Protaetia brevitarsis seulensis larvae activates RAW264.7 cells by the engagement of TLR4-JNK/NF-κB signaling pathway.

• Journal of Life Science
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• v.27 no.3
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• pp.334-338
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• 2017

In this study, the nutritional components of Tenebrio molitor, Protaetia brevitarsis, and Allomyrina dichotoma larvae, which have been registered as novel foods, were analyzed and compared to expand the diversity of selection criteria for edible insects. The contents of crude components, amino acids, fatty acids, and minerals were analyzed. According to the results of comparative analysis of edible insects, crude proteins were abundant in all three kinds of insects. The content of crude fat was the highest in T. molitor, and the content of carbohydrate was the highest in A. dichotoma. When comparing the composition of amino acids, total amino acid content and essential amino acids were the highest in T. molitor larvae. In T. molitor and P. brevitarsis larvae, the compositions of fatty acids were similar, with higher amounts of unsaturated fatty acids than in A. dichotoma. In terms of mineral content, A. dichotoma contained the highest amounts of calcium and iron, whereas P. brevitarsis contained the highest amounts of phosphorus and potassium. With these results, it is expected that edible insects could be selected according to nutritional demand. In addition, multiple combinations of edible insects will offer a richer intake of nutrients.

• Journal of Life Science
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• v.27 no.6
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• pp.702-707
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• 2017

Insects and their extract have been traditionally used as medicinal resources in Asia. Recently, Gryllus bimaculatus and the larva of Tenebrio molitor, Protaetia brevitarsis, and Allomyrina dichotoma were enlisted as general food ingredients by Korean Ministry of Food and Drug Safety. As the interest in these insects is growing, the detailed investigation is needed for the standardization of suitable and safe rearing techniques. This survey was conducted via personal interviews about edible insect rearing farms in Korea. The basic feed is fermented oak sawdust for P. brevitarsis and A. dichotoma and wheat bran for T. molitor. The various feed additives are blended with each farm's own recipes. The overall growth condition, including sawdust fermentation method, feeding period, and rearing density, varies among the investigated farms. It seems that weight of last instar larva was correlated with the rearing density and duration of the larval period. The heavy metal contents were analyzed after 48 hour starvation and they were very small amount or not detected in investigated insects. We believe that the present data will contribute to develop and standardize the safe rearing techniques of edible insects in Korea.

• Journal of the Korea Society of Computer and Information
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• v.24 no.12
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• pp.135-141
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• 2019

In this study, the "Insect Smart Farm Air Conditioning System" is designed and proposed for the control of breeding environment of Protaetia brevitarsis seulensis larvae. The proposed "Insect Smart Farm Air Conditioning System" separates the breeding room from the air conditioning room. It is a system that creates an environment optimized for breeding and distributes it into a breeding room. When controlling the environment through air-conditioning and humidifiers in insect farms, temperature and humidity vary from part of the breeding room to part. The solution to the problem can be suggested as a solution to the difficulty of producing white-spotted flower mounds of uniform size and weight when selling edible insects. By using the "Insect Smart Farm Air Conditioning System," the temperature difference can be reduced by 6℃ and the humidity difference by 24.7% compared to the environmental control of existing insect farms. The temperature and humidity of different parts of the breeding room were improved. Provide the optimal environment of Protaetia brevitarsis seulensis larvae at all times and ensure uniform CO₂ concentration. It can be expected to increase output through annual production and increase income for insect farmers. The proposed "Insecting Smart Farm Air Conditioning System" also controls the set temperature, humidity and CO₂. Environmental control of the breeding of other edible insects and the reproduction of mushrooms that require environmental control in breeding or breeding will also be possible.

• Journal of Life Science
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• v.27 no.10
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• pp.1185-1190
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• 2017

Insects are gaining recognition as an alternative source of protein. As a result, more and more domestic farms have begun mass rearing of edible insects. In order to produce high quality insects, studies on the development of safe and nutritious feed sources are needed. Given the cost of rearing insects, agricultural and industrial by-products are good sources for feed. The efficient utilization of these by-products can help in reducing the cost of production and preventing environmental pollution. In the current study, Citrus unshiu peel (CP), soybean curd cake (SCC), soybean oil meal (SOM), and brewers dried grain (BDG) were investigated for their effects on larval growth and development of Protaetia brevitarsis. Interestingly, the feed with 10% SCC increased larval weight by 3.5 times. For the larval period, the group of 10% SCC was significantly shorter than the control. Furthermore, minerals such as Zn, Ca, K, Mg, Na, and P were recorded to be high in 10% SCC. A total of 17 amino acids were present in 10% SCC, of which tyrosine and arginine were predominant. The heavy metal contents were very small amounts or not detected in any of the investigated groups. These findings provided a scientific basis for the utilization of soybean curd cake as a nutritional feed source to promote larval growth and produce quality insects.