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Deletion of N-terminal End Region of ErmSF Leads to an Amino Acid Having Important Role in Methyl Transfer Reaction (ErmSF에서 특이적으로 발견되는 N-terminal End Region의 점차적인 제거에 의한 활성에 중요한 아미노산의 규명)

  • Lee Hak Jin;Jin Hyung Jong
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.257-262
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    • 2004
  • ErmSF is one of the ERM proteins which transfer the methyl group to A2058 in 23S rRNA to confer the resis­tance to MLS (macrolide-lincosamide-streptogramin B) antibiotics on microorganism. Unlike other ERM pro­teins, ErmSF contains long N-terminal end region (NTER), of which $25\%$ is composed of arginine that is known to interact with RNA well. Gradual deletion of NTER leaded us to the point where mutant protein lost much of activity in vivo. Overexpressed and purified mutant protein showed much reduced activity in vitro: $2\%$ activity relative to that of wild type protein. This fact suggests that this amino acid interact with RNA close to meth­ylatable adenine to locate it at an active site properly.

Pacing Wire Removal Via a Right Atriotomy: A Patient with Infected Permanent Pacemaker and Who Previously Underwent CABG - A case report - (개심술을 통한 심박동기 와이어의 제거: 관상동맥 치환술을 받은 환자에서 심박동기 삽입 후 발생한 국소 감염의 치료 - 1예 보고 -)

  • Kim, Yun-Seok;Song, Hyun;Ryu, Yang-Gi;Jung, Sung-Ho
    • Journal of Chest Surgery
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    • v.42 no.2
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    • pp.256-258
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    • 2009
  • A 63-year-old man who had a permanent pacemaker placed 17 years ago presented to us with local inflammatory signs on his chest. The skin was opened to remove the permanent pacemaker. However, the pacing wire was not easily removed. So, the permanent pacemaker was removed from the chest with leaving the remnant wire inside. However, the local infection recurred because of the remnant wire. Right thoracotomy was performed and cardiopulmonary bypass was started. After total circulatory arrest, the right atrium was opened and the wire was completely removed.

An LBX Interleaving Watermarking Method with Robustness against Image Removing Attack (영상제거 공격에 강인한 LBX 인터리빙 워터마킹 방법)

  • 고성식;김정화
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.6
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    • pp.1-7
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    • 2004
  • The rapid growth of digital media and communication networks has created an urgent need for self-contained data identification methods to create adequate intellectual property right(IPR) protection technology. In this paper we propose a new watermarking method that could embed the gray-scale watermark logo in low frequency coefficients of discrete wavelet transform(DWT) domain as the marking space by using our Linear Bit-eXpansion(LBX) interleaving of gray-scale watermark, to use lots of watermark information without distortion of watermarked image quality and particularly to be robust against attack which could remove a part of image. Experimental results demonstrated the high robustness in particular against attacks such as image cropping and rotation which could remove a part of image.

태양전지용 단결정 실리콘 웨이퍼의 기계적 강도 및 결함 분포

  • Sin, Bong-Geol;Hyeon, Chang-Yong;Lee, Jun-Seong;Park, Seong-Eun;Kim, Dong-Hwan;Byeon, Jae-Won
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.450-450
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    • 2009
  • 최근 전세계적으로 태양전지의 대량보급에 따라 실리콘 원료의 공급에 차질이 생겨 원자재 값이 상승하는 추세에 있다. 결정질 실리콘 태양전지의 제조비용중 실리콘 재료 및 웨이퍼가 차지하는 비율은 약 50~60%정도로 높기 때문에 실리콘 웨이퍼의 두께를 감소시키는 것이 비용절감을 위한 효과적인 방법으로 기대되고 있다. 그러나 실리콘 웨이퍼의 두께가 앓아질수록 제조공정중 균열이나 파손이 발생할 가능성이 높아지기 때문에 이에 따른 실리콘 웨이퍼의 기계적 물성에 대한 연구가 필수적이라 할 수 있다. 본 연구에서는 현재 상용으로 사용되고 있는 크기가 5 인치인 $200{\mu}m$ 두께의 실리콘웨이퍼 (As-saw)를 약 80여개의 시편으로 절단한 후 각각의 파단강도를 부위별로 측정하였다. 또한 표면절단결함을 제거하는 saw damage etching(SDE) 시간을 제어하여 두께가 $150{\mu}m$, $130{\mu}m$인 웨이퍼를 준비하였다. 이들 시험편에 대해서도 부위별 파단강도를 측정하여 as-saw상태의 시험편과 비교하였다. 파단강도 측정은 4 접 굽힘시험을 통하여 측정하였으며 파단면은 주사전자현미경을 통하여 관찰하였다. 또한 실리콘 웨이퍼의 미세균열을 비파괴적으로 검출하기 위하여 100MHz 고주파수를 이용하는 초음파현미경(SAM, scanning acoustic microscope)을 이용하여 균열의 분포를 영상화하였다.

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Cutting Plane Algorithm for the Selection of Non-Isolated Pixel Modulation Code (고립 픽셀 제거 변조부호 선택을 위한 절단평면 알고리즘)

  • Park, Taehyung;Lee, Jaejin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.6
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    • pp.465-470
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    • 2013
  • In this paper, we introduce a modulation code design problem where best selection of two-dimensional codewords are determined to reduce two-dimensional (2D) Intersymbol Interference (ISI) and Interpage Interference (IPI), while when these codewords are randomly arranged on the storage, isolated pixel cannot be formed. Codeword selection problem and isolated pixel detection problem are formulated as integer program models and we develop a cutting plane algorithm where a valid cut is generated to remove current feasible solution to avoid isolated pixel by solving the isolated pixel detection subproblem. Using the proposed method, $4{\times}2$ 6/8 codewords with non-isolated pixel are found.

Dependence of High Affinity Binding of Epidermal Growth Factor on Receptor Cytoplasmic Domain (Receptor Cytoplasmic 영역에 의존하는 EGF의 고친화성 결합)

  • 강용호
    • KSBB Journal
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    • v.7 no.3
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    • pp.201-208
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    • 1992
  • Cell surface binding of epidermal growth factor(EGF) to EGF receptors was studied for a series of site-directed receptor mutants transfected into B82 mouse fibroblasts. Scatchard plots for truncation mutant receptors significantly lost nonlinearity for truncations below residue 1022. Transient plots of dissociation kinetics exhibited biphasic behavior for all receptor types, but the fraction of receptor in slow-dissociating form was reduced by an order of magnitude for the truncation mutants below residue 1022. Comparison of dissociation kinetics between control cells and cells treated with Triton X-100 revealed no significant variation for the slow-dissociating receptor form, but a noticeable variation was observed for the fast-dissociating receptor form when EGF receptors were truncated below residue 991. These results suggest that high affinity of EGF binding at cell surface depend on the EGF receptor cytoplasmic region.

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Effect of Natural Products on Skin Cells -Action and Suppression of Reactive Oxygen Species- (천연물의 피부세포에 미치는 영향 - 활성산소의 작용과 억제 -)

  • 박수남
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.25 no.2
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    • pp.77-127
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    • 1999
  • 활성 산소종은 노화, 특히 피부노화의 원인 물질로 작용하고 있다. 피부는 자외선에 노출되어 있어 활성 산소종을 만드는 광화학적 반응들이 계속해서 일어나고 있다. 이들 활성 산소종들은 피부 세포 및 조직 손상을 주도한다. 이들은 항산화 효소와 비효소적 항산화제들로 구성된 항산화 방어망을 파괴함으로써 산화제/항산화제 균형을 산화 상태 쪽으로 기울게 한다. 결과적으로 계속된 산화적 스트레스는 지질 과산화, 단백질 산화, 간질 성분을 파괴시키는 단백질분해효소의 활성화, 탄력 섬유인 콜라겐과 엘라스틴의 사슬절단 및 비정상적인 교차결합, 히아루론산 사슬의 절단, 멜라닌 생성반웅 촉진, DNA 산화와 같은 생체 구성 성분들의 손상을 야기시킨다. 결국에는 탄력감수 주름살 및 기미.주근깨 둥으로 특징 지워지는 피부노화가 가속화된다. 따라서 피부노화 방지를 위해서는, 과잉의 활성 산소종 생성을 억제하고 또한 생성된 활성산소를 효율적으로 제거할 수 있는 시스템이 화장품의 처방에 반드시 포함될 필요가 있다. 즉, 산화제/항산화제 밸런스가 유지되는 피부의 항산화 방어 시스템 구축이 필요하다. 피부노화 방지에 있어서 천연물의 역할로 (1) 자외선 흡수제로서의 역할, (2) 항산화제로서의 역할, (3) 주름 개선제로서의 역할, (4) 미백제로서의 역할, (5) 항균\ulcorner항염작용 및면역 조절제로서의 역할에 대하여 살펴 보았다. 21세기는 본격적으로 기능성 화장품 시대가 개막될 것으로 예측하고 있다. 이에 맞춰 천연물들은 피부노화를 방지하는데 주도적인 역할을 할 것으로 기대된다.

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Effect of Tuber Size, Bud and Tuber Removal, Tuber Cutting on Growth of Eleocharis kuroguwai Ohwi (괴경(塊莖)의 크기 및 절제(切除)가 올방개의 생육(生育)에 미치는 영향(影響))

  • Kim, J.C.;Son, S.Y.;Yu, K.R.;Yoon, J.S.
    • Korean Journal of Weed Science
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    • v.9 no.1
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    • pp.28-33
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    • 1989
  • This study was examined for the effect of tuber size, bud and tuber removal, and tuber cutting on growth of Eleockaris kuroguwai Ohwi. Elongation of plumule and radicle, and the weights of tuber, rhizome and shoot were increased as tuber size is large. The weights of shoot and tuber reduced by bud planting without tuber at 15 days after budding, however number of tubers were increased compared with normal tuber. When cross-cutting tubers were seeded, the weights of shoot, rhizome and tuber were produced as 84%, 85%, 71%, respectively compared with normal tuber, and the number of tubers was not influenced. The weights of tuber, rhizome and shoot were lowed as the bud removal, and there were a great number of tubers at bud removal of 15 days after budding. Tuber of E. k, contained as nonfibrous 78.9gr, phosphorous 77mg, iron 16.8mg, niacin 1.3mg per 100gr edble portion. The positive correlations were observed between shoot weight and tuber weight, and number of tubers.

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Design of Hierarchical Ring-Mesh Optical Networks Considering Cabling Cost (케이블 비용을 고려한 링메쉬 구조의 광통신망 설계)

  • Han, Jung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.5
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    • pp.1716-1729
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    • 2010
  • In this paper, we deal with a hierarchical ring-mesh optical network design problem. The objective is to minimize the total cost of optical add-drop multiplexers (OADMs) handling intra-ring traffic, optical cross-connects (OXCs) handling inter-ring traffic, and cabling cost among OADMs and among OXCs, while satisfying intra-ring and inter-ring capacities. We develop an integer programming (IP) formulation for the problem and devise some cutting planes that partially break the symmetry of rings. Dealing with the inherent computational complexity of the problem, we devise an effective heuristic procedure that finds a good quality feasible solution within reasonable computing times. Computational results demonstrate the efficacy of the proposed solution procedure; the developed symmetry breaking inequalities significantly reduce the computing time to find an optimal solution for small size problems, and the heuristic procedure finds a better feasible solution than that CPLEX, a commercial optimization software, finds for large size problems.

A Study on Contact Arc Metal Cutting for Dismantling of Reactor Pressure Vessel (원자로 해체를 위한 수중 아크 금속 절단기술에 대한 연구)

  • Kim, Chan Kyu;Moon, Do Yeong;Moon, Il Woo;Cho, Young Tae
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.21 no.1
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    • pp.22-27
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    • 2022
  • In accordance with the growing trend of decommissioning nuclear facilities, research on the cutting process is actively proceeding worldwide. In general, a thermal cutting process, such as plasma cutting is applied to decommissioning a nuclear reactor pressure vessel (RPV). Plasma cutting has the advantage of removing the radioactive materials and being able to cut thick materials. However, when operating under water, the molten metal remains in the cut plane and re-solidifies. Hence, cutting is not entirely accomplished. For these environmental reasons, it is difficult to cut thick metal. The contact arc metal cutting (CAMC) process can be used to cut thick metal under water. CAMC is a process that cuts metal using a plate-shaped electrode based on a high-current arc plasma heat source. During the cutting process, high-pressure water is sprayed from the electrode to remove the molten metal, known as rinsing. As the CAMC is conducted without using a shielding gas, such as Argon, the electrode is consumed during the process. In this study, CAMC is introduced as a method for dismantling nuclear vessels and the relationship between the metal removal and electrode consumption is investigated according to the cutting conditions.