• Journal of Life Science
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• v.16 no.2 s.75
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• pp.274-281
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• 2006

A fibrinolytic enzyme gene was isolated from Bacillus subtilis BB-1 by PCR method. Primers for PCR cloning were designed according to pre-identified gene for fibrinolytic enzymes from B. subtilis. The primer sequences were 5'-CGG ATC CGT GAG AGG CAA AAA GGT G-3' and 5'-TGA ATT CTT AAT GTG CTG CTG CTT GTC C-3' as concensus sequences of the fibrinolytic genes of Bacillus species. The PCR product was 1,145 bp and the sequence homology was 99% with nattokinase gene isolated from Japanese natto. The cloned fibrinolytic gene was reconstructed in Bacillus-E. coli shuttle vector, pEB for bulk-production. The fibrinolytic enzyme was purified by FPLC from the cloned B. subtilis 168. The optimum pH and temperature of the enzyme were 7.0 and $35^{\circ}C$, respectively. The fibrinolytic enzyme did not show any activity toward to skim milk, gelatin, casein and blood agar plate. The enzyme specific polyclonal antibody was prepared in rabbit for further assays such as detection of the gene expression in plant cells. This means that the enzyme may be used for health-care such as thrombosis without any hamful effects in the blood vessel.

• International Commerce and Information Review
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• v.19 no.1
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• pp.235-256
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• 2017

This study is to analyse how trust and asset specificity among partner firms affect on performance of international joint venture(IJV). Especially, the analysis was mainly based on a quadratic model. While it assumes that the previous studies was based on linear model in the relationship between trust, asset specificity and the performance, this study proceeds a empirical analysis by setting up a hypothesis; it would be quadratic relationship between trust, asset specificity and performance which are based on social capital theory and transaction cost theory. The survey was held with 74 manufactures who were established as an IJV by Korean and foreign firms together. In the result of the empirical analysis, trust shows an inverted U-shaped relationship with IJV performance. Also, asset specificity shows the U-shaped relationship with IJV performance. The results suggest that it needs to control and maintain the trust level among the partners in order not to lose an appropriate control caused by too much trust. In order to minimize the cost generated by asset specificity and to transform it into positive impact, it needs a control and the operation of monitoring system on the opportunistic action of the partners. Furthermore, it needs to keep organizational flexibility and innovativeness to continuously develop new capabilities.

• Korean Journal of Construction Engineering and Management
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• v.5 no.6 s.22
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• pp.90-100
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• 2004

The importance of information management has been emphasized in Korean construction industry, Both public and private have invested to establish and operate construction CALS, construction management (CM), computer integrated construction(CIC), and earned value management system(EVMS). A standard construction information classification system is essential to operate the systems mentioned above. Therefore, Korean government released Integrated Construction Information Classification System(ICICS) in 2001. However, the ICICS is not widely used in construction due to: 1) difficulty of changing existing system, 2) insufficient publicity of the ICICS, and 3) no legal binding force. Especially, participants in construction do not recognize applicability of the ICICS. This research surveyed the degree of recognition and utilization of the ICICS. This survey includes both customized classification systems used by companies and the ICICS and investigates the degree of utilization and drawbacks. The results show some construction companies use their own classification systems and the others use the ICICS prepared by the government. Furthermore, the degree of recognition is in sufficient. The degree of use in design management, specifications, cost and schedule management is very limited. The publicity and education are critical to induce the utilization of the ICICS. The necessity of revision was recommended based on pilot project study that is performed to measure the degree of application of ICICS on the projects. Therefore, this research proposes the measurement model for information application and analyzes the degree of utilization of the ICICS in different phases of construction.

• Korean Journal of Construction Engineering and Management
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• v.17 no.3
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• pp.134-142
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• 2016

Main infrastructures, such as levee, dam, bridge, road, etc., are very important due to not only the means of support for social and industrial activities in normal situation but also the means of protection of life and property during disaster occurrence. In spite of this importance of infrastructures, however, any disaster management systems that actively use these infrastructures are not developed yet. Moreover, infrastructures are not usually included in emergency action plans, thus it occurs second and third impact on communities and industries due to collapsing or damage of infrastructures. Therefore, the authors in this paper analyzed previous research, SWOT, STEEP, and patents and technical journals and conducted a technology need survey ni order to understand the trend of disaster management system as well as suggest main research fields and detail research items. The results of this paper will be a foundation of developing an advanced infrastructure integrated system of maintenance and disaster mitigation and contribute our nation to have an active response system by using infrastructure.

• Korean Journal of Construction Engineering and Management
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• v.17 no.3
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• pp.23-31
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• 2016

The domestic market of CM at Risk has not been activated yet. It was analyzed duo to the fact that construction owners didn't have confidence in CM at Risk, because of the concerns regarding CM firms reliability, service, specialty, system and feasibility. Therefore, this study analyzed the problems to find the reason for declines in orders through a business diagnosis based on the Business Model Canvas(BMC) and set up the direction of business model improvement with one on one expert interviews. Additionally, we suggested the sub-activities for the business model improvements of CM at Risk service that are divided into 9 building blocks. Through this method, we determined that we would need preceding innovation activities such as good communication with customers, reinforcement of subcontract and excellence in project operations to convert the successful practice and settlement of the CM business model based in BMC. It is expected that the business model suggested from this study would contribute to improvement of CM at Risk competition and the differentiation strategy when compared with other firms. Also, it would be used a basis data to develop CM at Risk business model in the future.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.1
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• pp.31-40
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• 2017

Absolute nodal coordinate formulation was developed in the mid-1990s, and is used in the flexible dynamic analysis. In the process of deriving the equation of motion, if the order of polynomial referring to the displacement field increases, then the degrees of freedom increase, as well as the analysis time increases. Therefore, in this study, the primary objective was to reduce the analysis time by transforming the dimensional equation of motion to a non-dimensional equation of motion. After the shape function was rearranged to be non-dimensional and the nodal coordinate was rearranged to be in length dimension, the non-dimensional mass matrix, stiffness matrix, and conservative force was derived from the non-dimensional variables. The verification and efficiency of this non-dimensional equation of motion was performed using two examples; cantilever beam which has the exact solution about static deflection and flexible pendulum.

• Proceedings of the Korean System Dynamics Society
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• 2004.08a
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• pp.87-107
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• 2004

우리나라에 이동통신이 처음 소개된 이래로 눈부신 발전을 거듭하여 왔으며, 급기야. 무선통신 서비스를 중심으로 새로운 성장력과 패러다임 전환의 가능성에 대한 전망을 논할 수 있는 수준이 되었다. 이러한 추세에 맞추어 휴대인터넷 시장에 대한 연구가 활발히 진행되고 있으며 국민경제적 효과변화나 시장 경쟁환경의 변화에 가장 영향을 미칠 수 있는 요인들 중, 사업자 수를 어떻게 선정할 것인가에 대해 다양한 접근이 시도되고 있다. 기존의 연구들은 휴대인터넷 시장을 분석하는데 있어 시장규모가 일방향으로 사업자 수에 영향을 미친다는 측면에서 이루어지고 있으며, 대부분 휴대인터넷 시장을 단일시장으로 범위를 한정하고 성장중인 시장을 정적으로 가정하여 시장성장 추이 분석 등에 주안점을 두는 단편적 연구가 수행되어져 왔다. 휴대인터넷 시장의 단편적 분석이 아닌 '모바일인터넷' , '초고속유선인터넷', '무선인터넷', '휴대인터넷' 등 네 가지 영역을 동시에 고려함으로써 영역간 복잡성과 동적인 관계 속에서 시장이 성장해 나아간다는 가정을 바탕으로, 시장에 내재되어 있는 관련요소간 상호영향과 신규정책 및 제도적 변화 수용에 있어 발생하는 시간적 공간적 지연 등을 고려한 동태적 분석을 수행하였다. 연구를 수행하기 위해 다양한 변수간의 인과관계, 피드백 구조와 시간흐름에 따른 시스템의 변화를 파악하는데 매우 유용한 도구인 시스템다이내믹스 기법을 활용하여 휴대 인터넷 시장의 동적인 구조를 알아보고 사업자 선정정책의 시행을 앞두고 있는 현재시점에서 의미있는 시사점을 제공하였다.시하고자 한다.채취하여 임신진단키트(제네디아프로테 트, 녹십자)를 이용하여 임신여부를 1차적으로 확인하였다. 과배란을 유기한 13두의 공란우중 9두(69.2%)가 과배란 반응을 나타내었으며, 회수된 수정란 51개중 이식가능수정란은 38개(74.5%) 였다. 발정동기화를 유도한 수란우 40두중에서 35두(87.5%)가 발정이 동기화되었으며, 그 중 황체검사를 통하여 30두의 수란우에 수정란을 이식하였다. 수정란이식후 13일(발정주기 21일)에 혈액을 이용한 임신진단에서 농가별 수태율은 각각 37.5%, 70.0%, 60.0% 및 71.4% 로서 평균 60.0%를 나타내었다.서 39$^{\circ}C$, 5% $CO_2$ 배양기에 48시간 배양하면서 생존여부를 판단하였다. 실험 2에서 확장배반포배 수정란이 25.3%의 생존율을 나타내었으며, 실험 1과 실험 3에서는 수정란의 형태와 관계없이 생존성을 확인할 수 없었다. 이상의 결과로 보아 glycerol 완만동결에서는 확장배반포기 수정란 이상이 보존가능한 것으로 추정되나 더 추가적인 연구가 요구된다.c kinase 활성의 변동은 정소 내 간충조직, 세정관 상피의 증식 및 기능적 분화 과정을 매개하는 생리적 활성분자 수용체 하위의 신호전달 과정에 Src-Csk loop에 의한 조절가능성을 확인할 수 있었다.rugrene의 향기성분이 주요 성분군으로 확인되었다. 2. 생강나무에서 생강의 향기를 발산하는 성분으로는 $\beta$-myrcene, o-terpinolene, phellandrone, ι-limonene, $\b

• Journal of Chest Surgery
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• v.33 no.11
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• pp.863-868
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• 2000

배경: Cox-Maze III (CM-III) 술식은 복잡한 심방 절개로 인한 긴 수술시간 때문에 다른 개심 수술과 병행하여 시행하기 어려운 단점이 있다. 대상 및 방법: 저자들은 CM-III 술직을 다음과 같이 변형하여 시행하고 그 임상성적을 분석하였다. \circled1 좌심방이를 절제하는 대신 좌심방이를 외부에서 결찰하고, \circled2 폐정맥 분리 절개선과 좌심방이 사이에 냉동절제술을 시행하며, \circled3 우심방이를 절제하는 대신에 우심방 외측 절개선을 우심방이까지 연장하고, \circled4 후종 우심방 절개 하부에서 우심방 외측을 지나 삼첨판막륜으로 향하는 T-자 절개선을 생략하였다. 저자들이 시행한 변형 술식의 용이성과 효율성을 평가하기 위하여, 우리나라에서도 빈도가 높은 류마치스성 승모판막 질환에서, 전통적인 Cox-III 술식(그룹 I)의 임상결과와, 변형된 CM-III 술식(그룹 II)의 임상결과를 비교하였다. 결과: 그룹 I(n=18)에서 동반된 수술은 승모판막 치환술 10례, 승모판막 성형술3례, 승모판막 치환술과 삼첨판막륜 성형술3례, 승모판막 재치환술 2례 등이었다. 그룹II(n=23)에서 동반된 수술은 승모판막 치환술 7례, 승모판막 성형술 5례, 승모판막 치환술과 삼첨판막륜 성형술 1례, 승모판막 재치환술 10례 등이었다. 그룹 I과 그룹 II에서 평균 대동맥 차단 시간(ACC)은 각각 135$\pm$29분과 104$\pm$18 분, 심패바이패스(CPB) 시간은 각각 240$\pm$33분과 185$\pm$42분이었다. 그룹 I과 그룹 II의 평균 추적 관찰 기간은 각각 47$\pm$14 개월과 29$\pm$4 개월이었다. 그룹 I에서는 16례(88.9%)에서 정상 동율동으로 회복되었고 1례에서 심방세동이 남아 있었으며, 다른 1례는 서맥증후군(sick sinus syndrome)으로 인공 심박조율기를 삽입하였다. 그룹 II에서는 21례(91.3%)에서 정상 동율동으로 회복되었고 2례는 심방세동이 지속되었다. 그룹 I에서 정상동율동으로 회복된 16례는 100%(16/16)에서 우심방의 수축을 심장 초음파검사에서 확인할 수 있었으며, 좌심방의 수축은 75%(12/16)에서 확인할 수 있었다. 그룹 II에서는 정상 동율동으로 회복된 21례 중 100%(21/21)에서 우심방의 수축을 확인할 수 있었으며, 좌심방의 수축은 76.2%(16/21)에서 확인할 수 있었다. 결론: 변형 CM-III 술식은 전통 CM-III 술식에 비하여 ACC time(p<0.005)과 CPB time(p<0.001)을 의미있게 줄이면서도 필적할 만 한 정상 동율동 전환율과 심방 수축력의 회복을 보여주었다.

• Journal of Korea Port Economic Association
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• v.24 no.2
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• pp.175-192
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• 2008

Today's individual firms no longer compete as solely autonomous entities, but rather as supply chain. As such the competitive position of a port is not only determined by its internal strengths but also it is also affected by its links in a global supply chin. In other words, port competitiveness is becoming increasingly dependent on external coordination and control of the whole supply chain. The main purpose of this paper is to examine how a port embeds itself into supply chain in order to strengthen its competitive position by focusing on Dubai port case. This paper found that Dubai port used three phases-insertion, integration and dominance-as a strategies for how it can embedded into global supply chain successfully. Dubai's global supply chain strategies give some implications for the further development of the Port of Gwangyang. First, the Port of Gwangyang should fully utilize symbiotic relationship with Gwangyang free Economic Zone. Second, the integration between Korea Container Terminal Authority and GYFEZ can be recommended for fast decision-making and providing a one-stop-service. Finally, Gwangyang should pursue an aggressive supply chain strategy, aims at dominance in the regional port network through port alliance with small and medium ports in neighboring area.

• Clinical and Experimental Pediatrics
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• v.48 no.11
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• pp.1166-1171
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• 2005

Purpose : The aim of this study was to evaluate whether breast milk feeding at infancy has the effect of a programming agent preventing school-age obesity, or whether formula milk feeding is the cause of elementary school-age obesity. Methods : We randomly selected 4 elementary school in Masan and Changwon city. We calculated the BMI and obesity degree from height and weight data on the school record of 1,275 children of first and second grade. The parents of 1,275 children were asked to a questionnaire about their birth history and feeding modality during infancy. Based on these data, we categorized them into 4- different groups : breast fed for less than 2 months; breast fed for 2-6 months; breast fed more than 6 months; mixed fed. We compared the mean BMI, obesity degree and prevalence rates of obesity of each groups. Results : There was no statistical difference on the prevalence rates of overweight and obesity, which was defined by BMI, according to the duration of breast feeding. The same was true for mild obesity and moderate obesity, which was defined by obesity degree. The mean BMIs and the mean obesity degree were not different among different feeding types. Conclusion : There seems to be no protective effect of breast feeding during infancy on reducing the prevalence rates of overweight and obesity. However, even wider and larger studies considering confounding factor might be needed to reach a definite conclusion in the future.