• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.375-380
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• 1987

D-$\alpha$-Amino-$\varepsilon$-carpolactam racemase (EC 5.1.1) and L-$\alpha$-amino-$\varepsilon$-caprolactam hydrolase (EC 3.5.2) were fractionated from cell-free extracts of Alcaligenes eutrophus A52 using ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. It was made sure that D-$\alpha$-amino-$\varepsilon$-caprolactam was converted to L-$\alpha$-amino-$\varepsilon$-caprolactam by racemase, and then hydrolyzed into L-lysine by hydrolase in Alcaligenes eutrophus A52. For the conversion of D-$\alpha$-amino-$\varepsilon$-caprolactam into L-lysine by cell-free extracts of Alcaligenes eutrophus A52, the optimum temperature and pH were 6$0^{\circ}C$ and 8.5 respectively. The results showed that 0.5% D-$\alpha$-amino-$\varepsilon$-caprolactam was converted to L-lysine at 55$^{\circ}C$ for 10 hr with a conversion rate of 98% by cell-free extracts containing 3.1mg of protein.

• Journal of Cadastre & Land InformatiX
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• v.45 no.1
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• pp.151-167
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• 2015

At present cadastral surveying is being driven fort combination of digitalized cadastral map accord with real cadastral boundary between cadastral resurvey area and World Geodetic System transformation about all country. So this study is conducted to newly establish items being needed in making digitalization of parcel boundary points based on the World Geodetic System in registration conversion surveying. For this study firstly management of mountainous districts Act, related laws and registration conversion-related laws and regulations are reviewd. Secondly, economical, administrative validation by using data from 12-branch companies of LX is analyzed. Thirdly, surveying method and procedure were established through experimental surveying on the two cases such as digital and analog area. Finally, through investigating standard of estimate about cadastral surveying, it was calculated amendment of registration conversion surveying fee based on World Geodetic coordinate System.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.284-287
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• 2003

Effects of ultrasound on cell growth and the production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) were investigated using transgenic Nicotiana tabacum cell suspension cultures. The culture suffered a slight growth depression immediately after the sonication, but gradually recovered to normal growth within 2 days. When the cells were exposed to ultrasound, the level of secreted hGM-CSF was 2.14 times higher than that in normal condition. From the beginning to 6 days of culture, production of secreted hGM-CSF was higher than that of control and then decreased. At the end of culture, however, hGM-CSF was considerably increased up to 36.7%. In the case of intracellular hGM-CSF, the level was slightly higher than that obtained in normal condition. Total hGM-CSF production was 31.5% higher than that of control culture after 6 days. The highest amount of hGM-CSF was $34.9\;{\mu}g/L$.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.293-297
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• 2003

Glutathione and ascorbic acid have been shown to fulfill many essential functions in animal and plant growth, development, defence and protection against oxidative damage. Effects of glutathione and ascorbic acid were examined in transgenic N. tabacum cells producing hGM-CSF to determine the effects of the vitamins on growth and cell viability. In lag phase, cell viability was preserved by glutathione and ascorbic acid. Therefore, recombinant protein productivity was increased. The purpose of present study is to investigate the role of antioxidants in cold stress-induced apoptosis in plant suspension cells. Cold stress lowered cell viability and increased total genomic DNA fragmentation. Supplementing the cell cultures with glutathione and ascorbic acid inhibited cold stress-induced decrease in cell viability and increase in total genomic DNA fragmentation.

• KSBB Journal
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• v.6 no.1
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• pp.27-33
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• 1991

Hairy root induced by A. rhizogenes from potato tuber (Solanm tuberosum L.) synthesized the agropine and mannopine which were demonstrated with paper electrophoresis. And the starch contents in hairy root were increased gradually following the developmental stage. But protein contents were decreased. The activity of ${\beta}-glucan$ synthetase II(GSII) which is related to the cell wall biosynthesis was stimulated in hairy root on the developmental stage. And chloropromazine did not influence the activity of GS II while verapamil inhibited about 60% of the activity GS II. Therefore, these results showed $Ca^{2+}$ to be effective factor in the cell wall formation. Isozyme pattern of peroxidase was investigated in the callus and hairy root induced from potato tuber.

• 한국어정보학회:학술대회논문집
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• 2016.10a
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• pp.195-200
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• 2016

본 연구는 MUSE 감성 코퍼스를 활용하여 문장의 극성과 키워드의 극성이 얼마만큼 일치하고 일치하지 않은지를 분석함으로써 특히 문장의 극성과 키워드의 극성이 불일치하는 유형에 대한 연구의 필요성을 역설하고자 한다. 본 연구를 위하여 DICORA에서 구축한 MUSE 감성주석코퍼스 가운데 IT 리뷰글 도메인으로부터 긍정 1,257문장, 부정 1,935문장을, 맛집 리뷰글 도메인으로부터는 긍정 2,418문장, 부정 432문장을 추출하였다. UNITEX를 이용하여 LGG를 구축한 후 이를 위의 코퍼스에 적용하여 나타난 양상을 살펴본 결과, 긍 부정 문장에서 반대 극성의 키워드가 실현된 경우는 두 도메인에서 약 4~16%의 비율로 나타났으며, 단일 키워드가 아닌 구나 문장 차원으로 극성이 표현된 경우는 두 도메인에서 약 25~40%의 비교적 높은 비율로 나타났음을 확인하였다. 이를 통해 키워드의 극성에 의존하기 보다는 문장과 키워드의 극성이 일치하지 않는 경우들, 가령 문장 전체의 극성을 전환시키는 극성전환장치(PSD)가 실현된 유형이나 문장 내 극성 어휘가 존재하지 않지만 구 또는 문장 차원의 극성이 표현되는 유형들에 대한 유의미한 연구가 수행되어야 비로소 신뢰할만한 오피니언 자동 분류 시스템의 구현이 가능하다는 것을 알 수 있다.

• Journal of Life Science
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• v.14 no.4
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• pp.657-663
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• 2004

Tobacco transformants harboring cinnamic acid 4-hydroxylase gene (C4H) fused with susquiterpene cyclase promoter was developed in order to regulate biosynthesis of phenolic compounds by the expression of the introduced gene. Twenty transformants for each specific promoter were used to analyze the incorporation of the chimeric genes by PCR and Southern blot analysis. PCR products of NPTII(neomycin phosphotransferase) gene (553bp) were detected in the transgenic tobacco plants. The incorporation of the chimeric gene was confirmed in the Southern blot analysis. C4H activity in the transgenic plants was elevated by UV-irradiation and its level was higher compared to that of control plants.

• Annual Conference on Human and Language Technology
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• 2016.10a
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• pp.195-200
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• 2016

본 연구는 MUSE 감성 코퍼스를 활용하여 문장의 극성과 키워드의 극성이 얼마만큼 일치하고 일치하지 않은지를 분석함으로써 특히 문장의 극성과 키워드의 극성이 불일치하는 유형에 대한 연구의 필요성을 역설하고자 한다. 본 연구를 위하여 DICORA에서 구축한 MUSE 감성주석코퍼스 가운데 IT 리뷰글 도메인으로부터 긍정 1,257문장, 부정 1,935문장을, 맛집 리뷰글 도메인으로부터는 긍정 2,418문장, 부정 432문장을 추출하였다. UNITEX를 이용하여 LGG를 구축한 후 이를 위의 코퍼스에 적용하여 나타난 양상을 살펴 본 결과, 긍 부정 문장에서 반대 극성의 키워드가 실현된 경우는 두 도메인에서 약 4~16%의 비율로 나타났으며, 단일 키워드가 아닌 구나 문장 차원으로 극성이 표현된 경우는 두 도메인에서 약 25~40%의 비교적 높은 비율로 나타났음을 확인하였다. 이를 통해 키워드의 극성에 의존하기 보다는 문장과 키워드의 극성이 일치하지 않는 경우들, 가령 문장 전체의 극성을 전환시키는 극성전환장치(PSD)가 실현된 유형이나 문장 내 극성 어휘가 존재하지 않지만 구 또는 문장 차원의 극성이 표현되는 유형들에 대한 유의미한 연구가 수행되어야 비로소 신뢰할만한 오피니언 자동 분류 시스템의 구현이 가능하다는 것을 알 수 있다.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.3
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• pp.510-518
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• 2014

In this study, in order to improve and optimize the performance of the turning mechanism for a fish robot in the fluid, we propose the tail joint trajectories using neural networks to mimic the CST(C-shape Sharp Turn) patterns of a real fish which is optimized in the natural environment. In order to mimic the CST patterns of a fish, we convert the sequential recording CST patterns into the coordinate data, and change the numerical coordinate data into a functions. We change the motion functions to the relative joint angles which is adapted to suit robot's shape and data. However, these relative joint trajectories obtained by the sequential recording of the carp have low-precision. It is difficult to apply to the control of a fish robot. Therefore, the relative joint trajectories are interpolated using neural networks with superior generalization ability and applied to the fish robot. we have found that the proposed method using neural networks is superior to ones using high-order polynomial equation through the computer simulations.

• Journal of Aquaculture
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• v.15 no.1
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• pp.15-21
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• 2002

Two groups of juvenile flounders (90 g/each) were fed on moist pellet feed thrice a day for a period of 8 weeks; the first group, reared in 4 circular (6m $\times$ 0.8 m) tanks (1200 flounders/tank), was fed daily (ED) and the second one on alternate days (EO). There was no significant difference in growth and feed conversion ratio (FCR) between these groups. There was also no difference in chemical composition, except in lipid, which was significantly higher (4.1%) in the ED group than the EO group (3.4%). Food intake and gain in the whole body nitrogen (N) were higher in the ED group but there was no significant difference between these groups in N retention; they excreted almost equal amount (114 vs 112 g) of N. Although phosphorus intake was significantly different between these groups, the flounders retained equal amount (0.5 g) of P in their body and excreted also equal amount (27.6 vs 26.0 g/kg weight gain) of p. Energy intake (2528 kJ) of the ED group was higher than that (2116 kJ) of the EO group. However, there was no significant difference in their energy retention efficiency. Clearly, alternate day feeding regimen led to more efficient utilization of food by the juvenile flounder.