• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.229.2-229.2
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• 2015

전자현미경은 전자빔을 이용하여 나노 수준의 분해능으로 초미세 구조물을 관찰할 수 있는 측정 장치이다. 이러한 전자를 소스로 사용하는 현미경에서 전자총의 특성을 파악하는 것은 전자현미경의 광학계를 설계하거나 그 성능을 평가할 때 매우 중요하다. 본 연구에서 제작한 전자총 평가 시스템은 전자총의 특성인 각전류밀도와 가상 전자원 크기를 측정할 수 있다. 이러한 특성을 정확하게 도출하기 위해서는 우선 가상 전자원의 위치를 알아야 한다. 전자총 평가 시스템은 전자총에서 방출된 전자빔을 형광 스크린에 조사하여 전자빔을 가시광선으로 변환하고, 형광 스크린 반대편에서 광학 현미경 렌즈가 장착된 카메라를 이용하여 빛으로 변환된 전자빔을 촬영하여 영상으로 획득 할 수 있다. 본 발표는 이렇게 획득한 영상에서 MathWorks(R)사의 MATLAB(R) 소프트웨어를 이용하여 물리적인 거리를 도출하기 위하여 사용하는 영상처리기법을 소개한다. 사용한 영상처리는 픽셀을 기반으로 계산하였으며, 취득 영상의 잡음을 제거하는 방법, 형광 스크린에서 획득한 전자빔에서 전자빔의 중심점 찾는 방법 및 이동한 전자빔의 거리를 계산하는 방법 등이 있다.

• Journal of the Korean GEO-environmental Society
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• v.11 no.12
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• pp.13-18
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• 2010

This study was made on the fact that the environmental impact and durability of the recently developed alkali silicasol chemical grout material. The grout material used for this study was designed to understand its environmental impact and durability through the SEM, chemical resistance test, leaching test, permeability test. In order to compare with the engineering characteristics regarding alkali silicasol grout material and sodium silicate grout material. As a result of SEM, the surface and internal tissues of alkali silicasol grout material could be identified to be denser than those of sodium silicate. As a result of leaching test the adaptability was identified as grout material as it had proved to be an ecological material owing to the total amount of the element to be leached being extremely little. As a result of permeability test it is judged that it is possible to apply the silicasol to the site in the place requiring the water cut-off as the silicasol.

• Journal of the Korean Society of Propulsion Engineers
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• v.10 no.1
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• pp.44-53
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• 2006

In this study, the non-destructive computed tomography was adopted to observe the density profile of the needle-punched Carbon/Carbon(C/C) composites nozzle throat. The density profile of C/C was evaluated within ${\pm}0.01g/cm^3$ with 98.74% confidence when the correction of the image and high signal-to-noise ratio were achieved by the optimization of the beam hardening, the electrical noise and the scattered X-ray. The density variation of C/C with the computed tomography was in good agreement with the results obtained by the water immersion method and the observation with scanning electron microscope.

• Proceedings of the Korean Information Science Society Conference
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• 2012.06a
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• pp.271-273
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• 2012

칩을 분석하는 과정에서 게이트의 검출도 중요하지만 그와 동등하게 중요한 부분이 wire의 인식이다. 본 논문은 wire의 자동인식의 방법에 관한 것으로, 칩 내부를 전자현미경으로 촬영한 사진을 기반으로 wire를 추출한다. 사람이 수작업으로 일일이 그 wire들을 그린다면 시간과 정확성이 떨어지므로 wire 인식 과정을 자동화하는 방법을 제시한다.

• Proceedings of the Korea Multimedia Society Conference
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• 2003.11b
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• pp.561-564
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• 2003

일반적으로 피부 미용분야에서 건강모발과 손상모발을 구분하기 위해 전자현미경으로 촬영된 영상에서 모발의 벌어짐, 갈라짐, 젖혀짐, 탈락, 용해 등의 특징을 관찰하여 사용하고 있다. 그러나 주관적이고 정성적인 판단에 의존하기 때문에 수치적인 기준을 적용하는 객관성이 있는 판단이 요구된다. 본 논문에서는 영상처리 분야에서 에지 특성이 영상 분류나 검색에 사용되는 점을 활용, 에지처리된 영상의 구간별 밝기 누적정보를 비교하여 건강모발과 손상모발의 구분이 가능함을 보인다.

• Journal of the Korea Convergence Society
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• v.8 no.8
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• pp.197-202
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• 2017

Three-dimensional melanin granules of mummy's hair analyzed using high voltage electron microscopy at 1250kV. The melanin granules in the cortex of mummy's hair located in close proximity to the outer of cortex in the adjacent place of the cuticles. A lot of melanin granule performed in this area. It is a distinguished difference from modern human. While it rotated up to 60 degree counter-clockwise and taken a photo per one degree using high voltage electron microscopy. The melanin granule displayed with long elliptical and variable at the size. The size of granule is measured from $0.3{\sim}0.6{\mu}m$ in minimum diameter and $0.5{\sim}1{\mu}m$ in maximum diameter. Conclusionally, high voltage electron microscope has higher resolution and penetration power than conventional transmission electron microscope that could be load biological thick specimen.

• Applied Microscopy
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• v.31 no.1
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• pp.1-8
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• 2001

The morphological features of neuronal dendritic spines are changed their shapes, sizes and density in response to physiological or pathological conditions . Therefore, exact analysis of spines warrants understanding of neuronal function. The size of the spine is at the borderline of resolution with light microscopy. High voltage electron microscopy Provide excellent resolution of the spines with proper stain techniques thanks to its higher resolution and penetration power. We evaluated more effective staining method for observing dendritic spines after labeling Purkinje cells with anti-calbindin 28 kD immunohistochemistry or Golgi staining methods. 4 fm thickness sections were observed with high voltage electron microscopy and some morphometric analyses were performed. Both Golgi staining and immunohistochemistry revealed the detail structures of the Purkinje cell such as soma, dendrites, and dendritic spines. High voltage electron micrographs with Golgi staining provide more precise morphology and are easy to measure. Average density of spine is $24.5{\pm}3.6/10{\mu}m$ and its length is $1.12{\pm}0.22{\mu}m$. For quantitative analysis of the spines, high voltage electron, micrographs with Golgi staining are more effective. This preliminary result is expected to be useful for further study of spine plasticity in various conditions.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2005.11a
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• pp.469-474
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• 2005

Tn this study, the noll-destructive computed X-ray tomography was adopted to observe the density distribution of the needle-punched C-C composites nozzle throat. The density distribution of the C-C was evaluated within ${\pm}0.01g/cm^3$ with 98.74% confidence when the correction of the image and high signal-to-noise ratio were achieved by the optimization of the beam hardening, the electrical noise and the scattered X-ray. The density variation of the C/C with the computed tomography was in good agreement with the results obtained by the water immersion method and the observation with scanning electron microscope.

• Journal of the Korea Computer Graphics Society
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• v.23 no.4
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• pp.31-40
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• 2017

As 3D cell culture has recently become possible, it has been able to observe a 3D shape of cell and volume. Generally, 3D information of a cell should be observed with a special microscope such as a confocal microscope or an electron microscope. However, a confocal microscope is more expensive than a conventional microscope and takes longer time to capture images. Therefore, there is a need for a method that can reconstruct the 3D shape of cells using a common microscope. In this paper, we propose a method of reconstructing 3D cells using the focus estimator value from multi-focal fluorescence images of cells. Initially, 3D cultured cells are captured with an optical microscope by changing the focus. Then the approximate position of the cells is assigned as ROI (Region Of Interest) using the circular Hough transform in the images. The MSBF (Modified Sliding Band Filter) is applied to the obtained ROI to extract the outlines of the cell clusters, and the focus estimator values are computed based on the extracted outlines. Using the computed focus estimator values and the numerical aperture (NA) of the microscope, we extract the outline of the cell cluster considering the depth and reconstruct the cells into 3D based on the extracted outline. The reconstruction results are examined by comparing with the combined in-focus portions of the cell images.

• Proceedings of the KAIS Fall Conference
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• 2004.11a
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• pp.93-95
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• 2004

가공방법에 따라 금형의 성능과 수명에 영향을 준다. 이와 같은 원인을 규명하기 위한 기초 연구를 수행하였다. 재료로는 프레스 금형 제작에 주로 사용되는 STD11을 선정하였다. 가공방법을 WEDM, WEDM후 템퍼링 등 2개 조건으로 하였다. 2개의 가공방법에 의해 제작된 시편을 전자주사현미경으로 표면, 단면조직사진을 촬영하였고, EDS를 하여 표면성분의 변화량을 측정하였다.