• The Korean Journal of Malacology
• /
• v.30 no.4
• /
• pp.353-361
• /
• 2014

In order to investigate the effects of copper alloy on abalone physiology, we studied survival rate, respiration, excretion rate, and heavy metal accumulation in each organ of adults and spats. The survival rate of spats and adults showed 27-60% and 63-83% respectively, higher survival rate in adults. In particular, 100% of copper panel led to lowest survival rate and there was no sharp distinction according to copper alloy composition. The respiration rate and excretion rate of ammonia nitrogen was $1.81mgO_2/g$ D.W./h and 0.43 mg $NH_4-N/g$ D.W./h respectively at 100% of copper panel. In other words, there was a high significant difference at the level, but no significant difference at other test levels (P < 0.05). The atomic ratio (0: N) hit the lowest at the 100% of copper panel showing 3.79 and no significant differences were seen among other test groups with 6.57-7.18 of a very low range. This means that the species might have undergone nutritional stress. In case of copper accumulation, the 100% copper panel group showed the highest level in hepatopancreas and muscle showing 6.91 mg/kg and 1.60 mg/kg respectively but the rest of groups showed similar levels. Zinc accumulation raised at Cu-Zn alloy panel had high significance showing 18.50 mg/kg and 1.10 mg/kg in hepatopancreas and muscle respectively (P < 0.05). To sum up, a cage net made of 100% pure copper is expected to have a negative effect on abalone in light of survival rate, heavy metal accumulation, and atomic ratio (0: N). Moreover, given that the substratum used for the high adhesive species and nutritious stress that is represented through the atomic ratio need to be considered, the copper alloy net is thought not to be suitable for abalone aquaculture.

• The Korean Journal of Malacology
• /
• v.26 no.2
• /
• pp.157-163
• /
• 2010

This study was carried out to investigate the toxic effect of WSF (Water Soluble Fraction) on development time, development rate, attachment rate, survival rate and growth of the larvae during the early life stage of the abalone, Haliotis discus hannai. Also, observed effect of water temperature on the survival rate and histological change of gill in the early young shell. In the abalone, it takes around 12 hours in all experimental groups. Development and attachment rate of the abalone significantly lower more than 0.4 mg/L WSF compared to control group (P < 0.05). Survival rate of abalone larva and spot was significantly lower more than 0.4 mg/L and 2.4 mg/L WSF compared to control group, respectively (P < 0.05). Shell growth of the abalone were significantly lower more than 2.4 mg/L WSF compared control group (P < 0.05). Survival rate lower more than $25^{\circ}C$ exposure group compared water temperature $17^{\circ}C$ exposure group in the early young shell. The gill of abalone exposed water temperature $17^{\circ}C$ and $25^{\circ}C$ was showed atrophy of nucleus and breakdown of the filament, vacuolation of filament epithelial cell.

• The Korean Journal of Malacology
• /
• v.30 no.3
• /
• pp.235-242
• /
• 2014

This study was conduct to investigate the effect of Intermediate culture types on the growth and survival rate of the abalone, Haliotis discus hannai, in net cage and indoor tank. Intermediate cultures were to determine there that was to setting at marine net cage culture (NCC) in net cage, floor culture (FC), net floor culture (NFC), double shelter culture (DSC) and indoor net cage culture (INCC) in indoor tank, in two replicate. In the growth performance of juvenile abalone reared through intermediate culture, that the absolute growth rate ($AGR_{SL}$, $AGR_{SB}$), daily growth rate ($DGR_{SL}$, $DGR_{SB}$), and specific growth rate ($SGR_{SL}$, $SGR_{SB}$) to the shell length $(_{SL})$ and shell breadth $(_{SB})$ of NCC were higher than those of different groups (P < 0.05). As weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to body weight through intermediate culture types in indoor tank was not significant. Also that, survival rate among experimental groups of intermediate culture in indoor tank was not significant. Therefore, these results is showed that should to cultivate for net cage so that intermediate culture of juvenile abalone over 2 cm, accordingly research to effective progress of juvenile abalone intermediate culture in indoor tank be should from various reason as well as feed and rearing condition.

• The Korean Journal of Malacology
• /
• v.28 no.2
• /
• pp.131-136
• /
• 2012

Growth, oxygen consumption, ammonia excretion, feeding and assimilation rate were examined from May 2010 to February 2011 in order to assess the physiological changes of Haliotis discus hannai in accordance with changes in season. The water temperature was in the range of $8-23.2^{\circ}C$ and the salinity in the range of 31.9-34.1psu during the examination period. The length of shell of Haliotis discus hannai grew from 36.3 mm to 66.1 mm in the 1 year old entities and from 60.6 mm to 66.1 mm for the 2 year old entities, while the weight of the meat increased from 3.16 g to 12.04 g and from 15.8 g to 21.5 g, respectively. The oxygen consumption and ammonia excretion rate displayed trend of increase in accordance with the increase in water temperature, while the feeding rate was high during the period from July to October. The assimilation rate was in the range of 68%-71% without significant difference between the age of the entities and seasons. SFG displayed +value throughout the year for the 1 year old entities of Haliotis discus hannai as well as for the 2 year old entities with the exception of the period of July, thereby showing that they are growing throughout the year. Therefore, there was no environmental effect including water temperature and salinity during the examination period.

• The Korean Journal of Malacology
• /
• v.31 no.2
• /
• pp.73-81
• /
• 2015

This study was conduct to investigate the effect of intermediate culture types on the growth and survival rate of the juvenile abalone, Haliotis discus hannai fed seaweed and artificial diet. Intermediate cultures were to determine there that was to fed seaweed (SW) of artificial diet (A) of floor culture (FC), net floor culture (NFC), double shelter culture (DSC) and indoor net cage culture (INCC) in land-based tank, in two replicate. In the growth performance of juvenile abalone reared through intermediate culture to fed SW of A, that the absolute growth rate ($AGR_{SL}$, $AGR_{SB}$), daily growth rate ($DGR_{SL}$, $DGR_{SB}$), and specific growth rate ($SGR_{SL}$, $SGR_{SB}$) to the shell length (SL) and shell breadth (SB) of experimental groups were not significant. As weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to body weight through intermediate culture types in land-based tank was not significant. However, as to survival rate to experimental groups, A-FC was higher than those of different groups (P < 0.05). Therefore, these results is showed that was not difference to growth of juvenile abalone over 2 cm fed seaweed diet and artificial diet according to intermediate culture types. But floor culture with artificial diet indicate that was highest to survival rate, therefore, it is beneficial for higher productivity in floor culture with artificial diet among intermediate culture types.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.18 no.6
• /
• pp.514-519
• /
• 2012

Abalone culture is one of important coastal fisheries off Wando in the southeast coast of Korea. Since cage culture for abalones was popularized, Understanding of temperature variation, which is important to raise abalones and to prevent their mass mortalities, is necessary. We analyzed temperature data from 2005 to 2009 obtained at Sinji-do and Cheongsan-do off Wando. Sinji-do, which is relatively close to land, had yearly wide range of temperature and rate of temperature variation. It is likely to be caused by heating of solar radiation in summer and cooling in winter at the shallow area. Rate of temperature variation in autumn was higher than that in spring. In summer short term variation of temperature corresponding tidal period was distinguished clearly. Diurnal temperature range, abrupt temperature change, was larger open sea. Comparison between temperatures of two stations and favorable raising conditions of abalones showed that Cheongsan-do, located out to sea, appears to be more appropriate than Sinji-do.

• The Korean Journal of Malacology
• /
• v.32 no.2
• /
• pp.95-102
• /
• 2016

This study investigated the growth characteristics of juvenile abalone when has been rearing as other different feed rates by the commercial abalone formulated feed on indoor tank, during the winter period that was maintaining on the low water temperature. Experimental abalones were use to 1 year old (shell length $29.14{\pm}2.56mm$, wet weight $2.9{\pm}0.6g$), and it has cultured at six feeding rate groups (0.75 DFW, 1.50 DFW, 2.25 DFW, 3.00 DFW, 3.75 DFW, 4.5 DFW) that were set up the daily feeding rate about total weight (DFW), and two replicated. The average water temperature in the experiment period was $9.7{\pm}3.27^{\circ}C$. In the monthly change absolute growth rate (AGRSL) and specific growth (SGRSL) of shell length, at January, 3.00 DFW was significantly higher than all feeding rate groups (P < 0.05). And in the monthly change of weight change and weight gain (WG), at March, 3.75DFW was significantly higher than all feeing rate groups (P < 0.05). The growth coefficient of thermal units (TGC) was decreased rapid since January, and 3.75 DFW was show significantly higher than all feeding rate groups (P < 0.05). In monthly change of feed efficiency (FE), at December, the 0.75 DFW was significantly higher than all feeding rate groups (P < 0.05), and in February and March, there was no significant difference between all feeding period. Therefore, In this study, was show that juvenile abalones can do to maintain or increasing from weight to supply commercial artificial diet during winter period when rearing into the indoor tank.

• The Korean Journal of Malacology
• /
• v.30 no.4
• /
• pp.383-390
• /
• 2014

The multiplex PCR system including six microsatellites from Haliotis discus hannai, consisting of dinucleotide and trinucleotide repeat units, is developed. The six loci were coamplified in a single reaction employing dye-labeled primers. Alleles from these loci were sized using an internal standard by automated sample processing in an ABI3100 Genetic Analyser. Amplified alleles in profiles containing selected microsatellites were typed clearly, providing easily interpretable results. In this results suggest that the presented multiplex PCR system may be a useful tool in a selective breeding program of H. discus hannai in which genetic identification will allow different genotypes to be reared together from fertilization. This should have a great impact as it will make selective breeding more efficient. Moreover, it will be useful in a variety of applications, including strain and hybrid identification, parentage assignment, pedigree reconstruction, estimating genetic diversity and/or inbreeding.

• Journal of Aquaculture
• /
• v.7 no.1
• /
• pp.9-20
• /
• 1994

To find out suitable indoor rearing tank shape and stocking density of the abalone Haliotis discus hannai, young abalones were reared in the square or ellipse shape tanks with various stocking densities and were fed dried sea mustard, Undaria Pinnatifida HARVEY for three years. There were no significant differences between the square rearing tank and the ellipse one in growth rate and survival rate. With regard to vertical design of rearing tanks with three floors, the significant differences were not found among culture floors. However, the growth and survival rates of young abalones from the running water system were significantly higher than those of the abalones from the circulating system (P< 0.05). For the stocking density experiment with 1,000, 1,500 and 2,000 individuals of 20 mm young abalone, there was no significant differences in growth and survival rates among these groups. However, in that of 200-400 individuals of 40 mm abalone and 100-300 individuals of 50 mm abalone, the lower stocking density of young abalone showed the higher growth and survival rates. The lower stocking density and the smaller size of young abalones showed the higher daily feeding rate and feed efficiency.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.13 no.1
• /
• pp.131-136
• /
• 2011

For the establishment of seed production technique of warm water abalone species Haliotis sieboldii, development of the fertilized eggs and its biological minimum temperature were determined. The durations of each development stages at the six rearing temperature regimes were expressed as an exponential equation: 4 celled stage 1/h = 0.1346T - 2.1709(r² = 0.88) Morula stage 1/h = 0.0176T - 0.2184 (r² = 0.89) Trochophore 1/h = 0.0063T - 0.0512 (r² = 0.98) Veliger 1/h = 0.0045T - 0.0295 (r² = 0.99) 2nd c.t. 1/h = 0.0008T - 0.0047 (r² = 0.99) According to the equation, the biological minimum temperature for Haliotis sieboldii was estimated to be 9.7 ℃.