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Water Quality and Hydrochemistry of Natural Springs and Community Wells in Daejeon Area (대전지역 자연샘물 및 공동우물의 수질 및 수리화학적 특성)

  • 정찬호;박충화;이광식
    • Economic and Environmental Geology
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    • v.35 no.5
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    • pp.395-406
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    • 2002
  • The sixty natural springs and community wells used as a drinking water in the Daejeon area are mainly located at the parks and the natural green districts. The purpose of this study is to investigate the characteristics of water quality and the contamination of the springs and the wells, and to suggest the management strategy for the springs and wells. For this study, we undertook water quality data from Daejeon City. According to the statistic analysis of water quality data, unacceptable rate as a drinking water was about 28 percent in 1999 and 24.5 percent in 2000, respectively. Major unacceptable factor is coliform, and others are bacteria, yersinia, color, turbidity, Fe and F. The unacceptable rate shows a roughly positive relationship with precipitation, that is, it shows highest rate during a rainy season between June and September. The major contamination source is likely to be the excrement of wild animals around natural springs and wells. Most of springs are vulnerable to the contamination of coliform and bacteria because of short residence time and shallow circulation in subsurface environment. The water samples collected from 31 springs or wells show weak acidic pHs, the electrical conductivity ranging from 63 to 357 $\mu\textrm{S}$/cm, and the hydrochemical types of Na(Ca)-HC0$_3$ and Ca-HC0$_3$. The groundwater samples of low total dissolved solid(TDS) belong to Na(Ca)-HC0$_3$. type, and the groundwater of high total dissolved solid is shifted towards Ca-HC0$_3$ type in the chemical composition. These hydrochemical characteristics indicate that most natural springs is in the early stage of geochemical evolution. The natural springs should be closed during a rainy season, which shows a high contamination rate. We suggest that a protection barrier around the springs should be built to keep wild animals away from the springs.

Effects of Compost Leachate on Growth and Yield of Leaf Lettuce in Hydroponic Culture (퇴비단 여과액비가 수경재배에서 상추의 생육과 수량에 미치는 영향)

  • Ryoo, Jong-Won
    • Journal of Animal Environmental Science
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    • v.15 no.1
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    • pp.51-58
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    • 2009
  • The compost leachate was dark-colored solution that leaches out of the bottom of the compost pile. The compost leachate was rich in nutrients and can potentially used in plant culture. In the organic production, commercial liquid fertilizer was used to insure the availability of nutrients during the formation of the yield. The cost of supplemental liquid fertilizer could be reduced by developing a fertilizer based on animal fertilizer. This experiment was conducted to investigate the effect of different combination of compost leachate and conventional inorganic solution in hydroponic culture for lettuce growth. Six different treatments were applied. The compost leachate(CL) and nutrient solution(NS) were mixed by six different mixing ratios of 0:100, 20:80, 40:60, 60:40%, 80:20 and 100%:0% based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of leaf lettuce. The concentration of nutrient solution was adjusted 1.5 mS/cm in EC. The compost leachate was low in phosphorus(P), calcium(Ca), magnesium(Mg), but rich in potassium(K). The plant height of lettuce treated with CL 20+NS 80% was similar with 100% NS of control plot. Plant height was highest in the plot of CL 20+NS 80%. The treatment of 100% compost leachate was lowest in the growth characteristics of leaf lettuce. Number of leaf was very low in 100% compost leachate compared with plot of chemical nutrient solution. In the beginning of growth stage, SPAD value was reduced in plot treated with CL100%, but CL20+NS80% plot was higher compared to 100% compost leachate. SPAD value of leaf lettuce leaves was decreased as the amount of CL was increased. The dry weight of lettuce were 107.4, 104.2g in plot of NS 100% and CL 20%+NS80%, respectively. The leaf number and plant weight were decreased at high application plots of compost leachate. The leaf lettuce showed lowest in the plot treated with 100% compost leachate, and the growth of lettuce severely decreased after application of 100% CL treatment. The results showed that compost leachate can be use as liquid fertilizer for the organic hydroponic production. The mixture solution of 20% of compost leachate and 80% of nutrient solution could be used as a nutrition solution in hydroponic culture of leaf lettuce. Our studies have shown that is possible to produce using compost leachate, although growth is slower than when using a conventional inorganic hydroponic solution.

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Immuno-stimulating Activities of Polysaccharides Isolated from Commercial Soy Sauce and Traditional Korean Soy Sauce (시판양조 및 재래식 조선간장으로부터 분리한 다당의 면역증강 활성 비교)

  • Park, Hye-Ryung;Lee, Moon-Su;Jo, Sun-Young;Won, Hye-Jin;Lee, Hyun-Sun;Lee, Ho;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.44 no.2
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    • pp.228-234
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    • 2012
  • The varying characteristics between traditional and commercial soy sauce may be initiated by raw materials and fermentation techniques for the production of $meju$ and $koji$. We examined properties regarding polysaccharides isolated from commercial soy sauce made by the $koji$ process (CSP-0) and Korean traditional soy sauce made by the $meju$ process (KTSP-0) as well as their immuno-stimulating activities. KTSP-0 had rhamnogalacturonan II (RG-II) including 1.1% of unusual monosaccharides 3-deoxy-D-$manno$-2-octulosonic acid (KDO). Anti-complementary activities of CSP-0 and KTSP- 0 were increased dose-dependently but KTSP-0 (64.7%) was higher than CSP-0 (56%) at $1,000{\mu}g/mL$. C3 activation products were identified by crossed immuno-electrophoresis. CSP-0 caused complementary activations $via$ only classical pathway while KTSP-0 caused complementary activations $via$ both alternative and classical pathways. KTSP-0 significantly increased the secretion of interleukin (IL)-6 at $8-1,000{\mu}g/mL$ and IL-12 at $40{\mu}g/mL$ on macrophages. The results suggest that the immuno-stimulating activity of KTSP-0 is greater than that of CSP-0 from anti-complementary activity.

Analysis of Daily Milking Flow in Holstein Dairy Cow Using the LactoCorder (전자식유량계를 활용한 홀스타인 젖소의 비유형질 분석)

  • Cho, Kwang-Hyun;Choi, Jun-Pyo;You, Byung-Wha;Lee, Deuk-Hwan;Kong, Hong-Sik;Park, Kyung-Do;Lee, Hak-Kyo
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.265-272
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    • 2009
  • A total of 486 milk records were collected from 16 diary farms in Imsil-gun, Jeollabuk-do. Results obtained were as follows: The average 3MG (amount of milk within the first three minute) was 7.44 kg and 55% of total milk yield was produced within 3 min. The average of SPL (% of foam in milk) was 33.93% and the average of MNG (strip yield) was 0.14 kg, which was less than 1% of total milk yield. The averages of HMF (highest milk flow), HMG (maximum milk flow rate in one minute) and DMHG (average milk flow in the main milking phase) were 3.03 kg/min, 2.94 kg/min and 2.05 kg/min, respectively and the average milking speed in Imsil-gun was slower than other regions. The average of tS500(time to reach 0.5 kg/min at beginning) was 0.23min (about 14 seconds) and that of tMGG (duration of the total milking) was 7.75min. The average tMBG (duration of the dry milking phase) was 0.58 min (35 seconds) and that of tMNG (duration of the stripping phase) was 0.42min (14 seconds). The averages of ELHMF (electrical conductivity at highest milk flow) and ELAP (beginning peak level of the electrical conductivity) were 6.81 mS/cm and 7.58 mS/cm, respectively. The average of ELMAX (maximum electrical conductivity) was 7.48 mS/cm and that of ELAD (beginning peak difference of the electrical conductivity) was 0.61 mS/cm. While the total milk yields for DMHG, tMHG (duration of the main milking phase), tPL (duration of the plateau phase), tAB (duration of the descending phase) and tMGG were positively correlated (0.35~0.54), those for tMBG and SPL were negatively correlated (-0.11 and -0.27). As the DMHG increased, tMHG, tPL, tAB, tMGG and SPL decreased. While the cows with higher electrical conductivity at the beginning of milking had less somatic cell counts, cows with higher electrical conductivity after the peak of milk yield had more somatic cell counts. The results of this experiment indicated that through milking based on milking and lactating standards and the regular checking of milking status, the qualities of milk and milk yields could be improved.

Developmental Stage and Temperature Influence Elongation Response of Petiole to Low Irradiance in Cyclamen persicum (저광도에 대한 시클라멘 엽병의 발육 단계 및 온도 조건별 신장 반응)

  • Oh, Wook;Kim, Ki-Sun
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.719-727
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    • 2010
  • Reduced irradiance promotes shoot elongation depending on developmental stage and environmental factors and decreases plant quality in $Cyclamen$ $persicum$ Mill. To determine the petiole elongation responses to low irradiance, 'Metis Scarlet Red' cyclamen at different developmental stages [juvenile (5-6 unfolded leaves), transitional (1-3 visible flower buds), or mature (1-3 elongating peduncles)] was grown in growth modules at 60 (low light, LL) or 240 (high light, HL) ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD within the growth chambers at different temperatures [16/12 (low temperature, LT), 22/18 (medium temperature, MT), or 28/$24^{\circ}C$ (high temperature, HT) (day/night)]. In Experiment I, juvenile plants were either kept in an LL or HL module during the entire treatment of 4 weeks or were transferred to the other module at 1, 2, or 3 weeks after treatment in an MT chamber. In Experiment II, juvenile, transitional, or mature plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the MT chamber and grown for 21 days. In Experiment III, transitional plants were moved to the HL module at 0, 3, 6, 9, or 12 days after being placed in the LL module at the LT, MT, or HT chambers. As the exposure duration to LL increased from 0 to 4 weeks or from 0 to 12 days, petiole length and plant height increased at all temperatures and developmental stages. In Experiment I, the exposure to LL during the latter period, rather than the early period, increased elongation rate. In Experiment II, petiole elongation in transitional plants was more sensitive to LL than juvenile or mature plants during the early period of the treatment for 12 days. In Experiment III, petiole length increased with increasing temperature and exposure duration to LL. Petiole elongation rate at HT increased rapidly from the beginning of LL exposure as compared to LT. Increase of $6^{\circ}C$ in temperature had the similar effect to LL exposure for 3 days in petiole elongation. To conclude, transitional cyclamen under higher temperatures responds more immediately to low irradiance and elongates its petioles.

Eruption Styles and Processes of the Dongmakgol Tuff, Cheolwon Basin, Korea (철원분지 동막골응회암의 분출유형과 분출과정)

  • Hwang, Sang Koo;Son, Yeong Woo;Choi, Jang O;Kim, Jae Ho
    • The Journal of the Petrological Society of Korea
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    • v.22 no.1
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    • pp.49-62
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    • 2013
  • The Dongmakgol Tuff is divided into 8 lithofacies based on their grain size and depositional structures: massive tuff breccia(TBm), welded tuff and lapilli tuff(LTw), rheomorphic tuff and lapilli tuff(LTr), massive lapilli tuff(LTm), stratified lapilli tuff(LTs), gradedly bedded lapilli tuff(LTg), crudely bedded lapilli tuff(LTb) and massive fine tuff(Tm). They can be divided into 3 pyroclastic rock group based on their constituents of the lithofacies. The lower group(LI) is composed of LTm, LTw and LTr, which are interpreted to have resulted from emplacement of voluminous pyroclastic flows due to ignimbrite-form eruption to boiling-over eruption. The middle group(LT+MI) consists of LTs, LTg and LTm associated with Tm in the lower part, and of LTm, LTw and LTr in the middle and upper parts; these suggest that started with deposition of pyroclastic surges from phreatoplinian eruption by poor eternal water, passed through emplacement of pyroclastic flows from ignimbrite-form eruption and ended with deposition of voluminous pyroclastic flows from boiling-over eruption. The upper group(lUT+uUT+UI) is composed of LTs, LTg and Tm in the lowermost, TBm, LTb, LTb and Tm in the lower part, and LTm and LTw in the middle and upper part, suggesting that began with deposition of surges from phreatoplinian eruption, passed through deposition of pumice- and ash-fallouts from plinian eruption and transformed into emplacement of pyroclastic flows due to boiling-over eruption. As result, eruptive processes in the Dongmakgol Tuff approximately began with phreatoplinian or/and plinian eruption, transformed into ignimbrite-forming eruption and proceeded into boiling-over eruption in each volcanism, but proceeded presumably without phreatoplinian or plinian eruption in the earlier stage of 1st volcanism.

Possibilities of Wasabia japonica Matsum Culture using Cold Water of the Soyang River Dam (소양강댐의 냉수(冷水)를 이용한 고추냉이 재배 가능성)

  • Lee, Sung-Woo;Seo, Jeong-Sik;So, Ho-Seob;Beon, Hak-Su;Park, Jang-Hwan;Kim, Suk-Dong
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.4
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    • pp.294-300
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    • 1996
  • We performed an experiment to confirm the possibility of wasabi culture using the wat­er of the Soyang River Dam in Chunchon and the ground water in Suwon. Water mineral content of Soyang River except for P was less than that of ground water of Suwon. Dis­solved oxgen and E C of Chunchon was proper to culture wasabi but E C and dissolved oxgen of Suwon was not suitable for that. Water temp. of Soyang river was very changable by month while that of the ground water in Suwon was kept constantly. In Soyang river of Chunchon the month that water temp. show $8{\sim}18^{\circ}C$, optimal growth temp., was May to Nov. and the month that water temp. show less than $6^{\circ}C$, growth limit temp., was $Jan.\;{\sim}\;Feb.$ of Chunchon. Rhizome weight of main stem in Chunchon and Suwon was 63g and 22g per plant and rate of maketable rhizome was each 80%, 0% by culture of 32 months to include raising see­dling period of 13 months. Dry matter partitioning ratio of petiole in Soyang river of Chunchon was the highest of all others but it was lowest of all others in ground water of Suwon. Rhizome weight of main stem in Chunchon was showed possitive correlation with plant height and fresh top weight and in Suwon it was showed possitive correlation with root weight and high possitive correlation with No. of total leaves and No. of tillers.

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Purification and Characterization of the Red Carotenoprotein from the Muscle of Blue Mussel, Mytilus edulis (진주담치 근육중의 적색 Carotenoprotein의 정제 및 특성)

  • YANG Huyn-Pil;LEE An-Jong;KIM Yong-Tae;KIM Se-Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.482-494
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    • 1994
  • Most of carotenoprotein complexes have been extracted by using buffered solutions. However, in this study carotenoprotein from the muscle of Blue mussel(Mytilus edulis) was extracted by a detergent such as Triton X-100. It was purified and characterized by $20\%$ (w/v) $(NH_4)_2SO_4$, DEAE-cellulose ion exchange and Sephacryl S-300 gel filtration. The carotenoprotein(${\lambda}_{max}=462nm$) had an approximate M. W. of 372KDa(gel filtration). SDS-PAGE analysis of the carotenoprotein indicated the presence of four polypeptides of 60KDa($23.70\%$), 46.9KDa($9.14\%$), 26KDa($49.14\%$) and 13KDa($18.02\%$). Carotenoprotein denaturated by treatment with SDS to a final concentration of $0.2\%$ (w/v) caused a hypsochromic shift of ${\lambda}_{max}$ from 462nm to 456nm. The carotenoprotein contained lipids as structure units. The amino acid composition of the carotenoprotein contained large essential amino acid amounts of $62.8\%$, and the content of threonine($35.9\%$) was higher than other amino acids, but histidine, methionine and proline were not present. In the carotenoprotein, the major fatty acids were $C_{16:4},\;C_{16:0},\;C_{20:5}\;and\;C_{22:6}$. The percentages of polyunsaturated fatty acids($62.4\%$) were higher compared to other fatty acids(saturated fatty acids $19.6\%$, monounsaturated fatty acids $18.0\%$). Carotenoid was extracted from the carotenoprotein by acetone and it was separated into five different components by preparative TLC(benzene:petroleum ether:acetone=69:17:14). The major components of carotenoid were mytiloxanthin($74.79\%$) and 3,4,3'- trihydroxy-7',8'-didehydro-${\beta}$-carotene($18.26\%$), and they were at least presented as prosthetic groups of carotenoprotein.

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MEASUREMENT OF PULPAL BLOOD FLOW USING A LASER DOPPLER FLOWMETER (Laser Doppler flowmeter를 이용한 치수혈류 측정)

  • Ban, Tae-Whan;Lee, Jae-Sang;Kim, Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.24 no.4
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    • pp.560-569
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    • 1999
  • Blood supply rather than nerve supply implies pulp vitality. To evaluate pulp vitality clinically, electric pulp test and thermal test which are based on sensory nerve response have been used in addition to many auxiliary data such as past dental history, visual inspection, radiographic examination, percussion, palpation and transillumination test. However, reactivity of the nerves to the stimulation is not synonymous with normalcy. Therefore measurement of pulpal blood flow using a laser Doppler flowmeter became a new trial to test the pulp vitality. The purpose of the present study was to evaluate normal pulpal blood flow level of maxillary teeth in adult to provide a guideline in determining the vitality of dental pulp. Pulpal blood flow was measured in maxillary central and lateral incisors, canines, first and second premolars and first molars of seventy nine adults of 22 - 30 years old using a laser Doppler flowmeter (PeriFlux 4001, Perimed Co., Stockholm, Sweden, 780 nm infrared laser, 1mW). For directly-made splints, silicone rubber impressions were taken directly from the mouth. For indirectly-made splints, alginate impressions were taken from the mouth and stone cast were made. After making depressions on the buccal surfaces of the cast teeth to indicate the hole positions, second impressions with vinyl polysyloxane putty were taken from the cast. Holes for the laser probes were made at the putty impressions 4mm above the gingival level. Laser probe (PF416 dental probe, 1.5mm) was inserted in the prepared hole and the splint was set in the mouth. After 10 minutes of patient relaxing, pulpal blood flow was recorded for 5 minutes on each tooth. The recorded flow was saved in the computer and calculated with a software 'Perisoft' version 5.1. Pulpal blood flow was also recorded in six teeth of five individuals with no response to electric pulp test and cold test, with periapical radiolucency, or with history of root canal treatment to compare with nonvital teeth. The difference between the mean flow values of each group of teeth were analyzed using one-way ANOVA and Duncan's Multiple Range test. The results were as follows: 1. The average pulpal blood flow values of all the tested teeth of each location were between 9 - 16 Perfusion Unit. Pulpal blood flow value was highest in maxillary lateral incisors, followed by first premolars, second premolars, canines, central incisors, and then first molars (p<0.01). 2. In six anterior teeth, indirectly-made splint group showed higher pulpal blood flow values than directly-made splint group (p<0.01). In posterior teeth, however, there was no significant flow value difference between directly-made splint group and indirectly-made splint one (p>0.05). 3. Teeth with vital pulps showed higher signal values than teeth with nonvital pulps (p<0.01), and the flow photographs showed heartbeat-synchronous fluctuations and vasomotions, while those were absent in non vital tooth.

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Egg Development and Larvae Morphology and Spawning Behavior of Five Striped Damselfish, Abudefduf vaigiensis (Pisces: Pomacentridae) Reared in the Laboratory (실험실에서 사육한 해포리고기 (Abudefduf vaigiensis)의 산란행동, 난 발생 및 자어의 형태발달)

  • Park, Jae Min;Jung, Hyun Ho;Han, Kyeong Ho;Cho, Jae Kwon;Kim, Na Ri;Kim, Jae Myoung;Baek, Jung Ik;Park, So Hyun
    • Korean Journal of Ichthyology
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    • v.25 no.2
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    • pp.82-89
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    • 2013
  • The spawning behavior, development of eggs and larvae of the Five striped damselfish, Abudefduf vaigiensis were studied. The Five striped damselfish were caught at Dolsan Island, Yeosusi, Jeollanamdo from May in 2011. As a result of observation, male fish attracted female after cleaning the rock. Female left after spawning and male protected their eggs until they had hatched out. The fertilized eggs were elliptical in shape (mean long diameter: 1.06 mm; mean short diameter: 0.55 mm) and transparent. Larvae hatched at 53 hrs after fertilization in $24.5{\sim}26.5^{\circ}C$(mean $25.0^{\circ}C$). The newly hatched larvae were 2.55~2.86 mm (mean 2.71 mm, n=10) in total length and their mouth and anus were already opened. They began to eat rotifer and transformed to postlarva stage. 3 days after hatching postlarva was measured 2.74~2.97mm(mean 2.84 mm, n=10) in total length. 10 days after hatching postlarva was measured 3.85~4.20mm(mean 4.00 mm, n=10) in total length with dosal fin rays IV-5; ventral fin rays I-3; caudal fin rays 1~2.