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The Growth and Physiological Responses of Cacalia firma Seedlings by Shading Conditions in Forest Farming (임간재배 시 병풍쌈 유묘의 차광처리별 생장 및 생리 반응)

  • Yoon, Jun Hyuck;Jeon, Kwon Seok;Song, Ki Seon;Park, Yong Bae;Moon, Yong Sun;Lee, Do Hyung
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.65-71
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    • 2014
  • Cacalia firma is a perennial plant in Asteraceae, Parasenecio that distributed in Korea, China, and Japan. As dietary style changes for well-being life, consumer's demand of functional food and organic vegetables is getting increased. This study was conducted to investigate the optimum light conditions of P. firmus in forest farming. One year old seedlings were grown under four different light conditions 10%, 20%, 30%, and 50% of sunlight by shading (equals 50%, 30%, 20%, and 10% relative brightness respectively) and non-treated control under full sunlight. They were analyzed for early growth and physiological response. Seedlings grown under 75% shading showed similar height, root growth, and leaf water content to control. However, their leaf length, width, and total leaf area were increased, which caused increased leaf dry weight and total dry weight. Especially, seedlings under 95% shading showed 40% increase in height and more leaf growth and leaf water content, although they had shorter main root length and root collar diameter than control. In addition specific leaf area (SLA) and leaf area ratio (LAR) were higher than control and indicated that they were statistically significant difference from control. Higher SLA refers thinner leaf thickness, higher LAR means larger leaf area. The results indicate seedlings under 95% shading have higher water content, thinner leaf, and wider lightinterception areas. It is plausible that P. firmus is active in chlorophyll activities and carbon dioxide assimilation at even lower light conditions. These results suggest that the optimum light level of P. firmus for artificial cultivation in forest farming ranges from 75~95% shading (20%-10% of relative brightness). When salability as 'sanchae' (wild edible greens) is considered, P. firmus could be cultivated under 75% shading in forest farming and expected to have better taste and higher yield. We suggest these results as basic data of P. firmus for possible forest farming.

Correlation Analysis between Climatic Factors and Radial Growth and Growth Prediction for Pinus densiflora and Larix kaempferi in South Korea (소나무와 일본잎갈나무의 연륜생장과 기후 요소와의 상관관계 분석 및 생장예측)

  • Chung, Junmo;Kim, Hyunseop;Kim, Meesook;Chun, Yongwoo
    • Journal of Korean Society of Forest Science
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    • v.106 no.1
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    • pp.77-86
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    • 2017
  • This study was conducted to analyze the relationship among climatic factors and radial growth of Pinus densiflora and Larix kaempferi in South Korea. To determine the climate-growth relationship, cluster analysis was applied to group surveyed regions by the climatical similarity, and a dendroclimatological model was developed to predict radial growth for each climate group under the RCP 4.5 and RCP 8.5 scenarios for greenhouse gases. The cluster analysis showed four climatic clusters (Cluster 1~4) from 10 regions for P. densiflora and L. kaempferi. The dendroclimatological model was developed through climatic variables and standardized residual chronology for each climatic cluster of P. densiflora and L. kaempferi. Four climatic variables were used in the models for P. densiflora ($R^2$ values between 0.38 to 0.58). Two to five climatic variables were used in the models for L. kaempferi ($R^2$ values between 0.31 to 0.43). The growth simulations with two RCP climate-change scenarios(RCP 4.5 and RCP 8.5) were used for growth prediction. The radial growth of the Cluster 4 of P. densiflora, the mountainous region at high elevation, tend to increase, while those of cluster 2 and 3 of P. densiflora, the region of the hightest average temperature, tends to decrease. The radial growth of the Cluster 1 of L. kaempferi the region of the lowest minimum temperature, while that of Cluster 2, the region of the highest average temperature, tends to decrease. The radial growth of Cluster 3 of L. kaempferi, the region in the east coast and Cluster 4, the region at high elevation, tends to hold steady. The results of this study are expected to provide valuable information necessary for predicting changes in radial growth of Pinus densiflora and Larix kaempferi caused by climate change.

A Study on the Intake and Satisfaction Levels of Busan Foods among Chinese Tourists (중국 관광객의 부산음식의 섭취현황 및 만족도에 관한 연구)

  • Lim, Yaung-Iee;Lee, Jong-Hyeon;Kim, Young-Joo;Jeong, Ji-Kang;Kim, Sin-Jeong;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.9
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    • pp.1492-1498
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    • 2013
  • This study was conducted to evaluate the intake of food and degree of satisfaction of services among Chinese tourists in the restaurants in Busan. The choice of what these tourists would eat after touring Busan was also determined. We chose 100 independent Chinese tourists visiting Busan for this study. Students, housewives, highly educated people, and people who visited once or twice were predominant among our study subjects. The Chinese tourists chose the following Busan local foods during their visit: Saengseonhoe (19%), Haemultang (19%), Oribulgogi (17%), Dwaejigukbap (12%), and Dongrae Pajeon (11%). The tourists responded that they would like to eat Bulgogi, Samgyeopsal, Bibimbap, Doenjangjjigae, and Dwaejigalbi again if they revisit Busan, indicating that they prefer general Korean food rather than Busan local food. The degree of satisfaction of Chinese tourists with Busan food was low and their response showed they were not satisfied with the prices and the amount of food served. Services (kindness, cleanliness) in Busan restaurants ranked high in the opinion of Chinese tourists. Of all the courses included in their tour of Busan, shopping for items received the highest score of satisfaction (44%), while food in Busan earned the lowest (10%). As regards intention to revisit Busan, 38% of the Chinese tourists answered yes, and 61% were unsure, and 1% was no. Our survey results indicate that restaurants in Busan city need to prepare food of choice for Chinese tourists and also make an effort to raise satisfaction on the cost and amounts of Busan foods.

Quality Characteristics of Black Raspberry Wine Fermented with Different Yeasts (효모의 종류를 달리하여 제조한 Black Raspberry 발효주의 품질 특성)

  • Lee, Yoonji;Kim, Jae Cheol;Hwang, Keum Taek;Kim, Dong-Ho;Jung, Chang Min
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.5
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    • pp.784-791
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    • 2013
  • Four different yeasts (Fermivin (FM), Saf-instant yeast red (SI), Angest wine active dry yeast (AW) and Angest instant yeast high sugar (AI)) were used for the fermentation of black raspberry wine. The amount of reducing sugars in FM (2.7%) and AI (2.8%) were higher than those in SI (2.4%) and AW (2.5%). The amount of glucose (the major free sugar) was higher in AW (2.57 mg/mL) and AI (2.50 mg/mL) than FM (2.03 mg/mL) and SI (1.75 mg/mL). AW (11.95%) had the highest alcohol content, while SI (11.75%) had the lowest. The pH of FM (pH 3.73) was the lowest, and there were no significant differences in total acidity among the samples. The major organic acid in the wines was citric acid (6.71~8.18 mg/mL) and the amount of organic acids depended on the type of yeasts. The amount of malic acid was highest in SI (2.92 mg/mL), and lowest in AI (1.83 mg/mL). The Hunter color test showed that SI was highest in lightness, redness and yellowness, whereas AI was lowest. There were no differences in turbidity between the samples. There were no significant differences in total phenolic contents (TPC) and total anthocyanin contents (TAC). However, the TPC and TAC of black raspberry wines were higher than those in commercial red wines. The antioxidant activities of wines (determined by ABTS and FRAP) increased in the order of FM, AI, AW and SI. It could not be concluded which yeast is adequate for the fermentation of black raspberry wine because any of the tested yeasts showed the best in all the quality characteristics of the wines.

Induction of Apoptosis and Inhibition of Growth in Human Gastric Cancer by Piperine (Piperine에 의한 위암세포 AGS 증식 억제와 Apoptosis 유도)

  • Shin, Seong-Ah;Lee, Hae-Nim;Choo, Gang-Sik;Kim, So-Jung;Kim, Hyeong-Jin;Park, Young-Seok;Park, Byung-Kwon;Kim, Byeong-Soo;Kim, Sang-Ki;Lee, Hu-Jang;Jung, Ji-Youn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1589-1594
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    • 2016
  • Piperine [(E,E)-5-(3,4-methtylenedioxyphenyl)-2,4-pentadienolypiperidide] is a principal of Piperaceae, including Piper nigrum L. and Piper longum Linn., which has been used as a spice and traditional medicine. In this study, we investigated whether or not piperine has anti-cancer effects on AGS human gastric cancer cells. The results demonstrated that piperine not only inhibited proliferation using MTT assay but also induced apoptotic bodies using DAPI assay in a dose-dependent manner in response to piperine. Expression levels of p53, Bax (pro-apoptotic), cleaved caspase-9, and cleaved-PARP increased, whereas expression levels of Bcl-2, XIAP (anti-apoptotic), and Akt decreased in a dose-dependent manner compared with the control by western blotting analysis. To identify the connection between phospo-Akt and Bcl-2 family in response to piperine, LY249002 (Akt inhibitor) was treated with piperine ($150{\mu}M$). The results were shown that expression of phospo-Akt was reduced whereas expression of Bax and cleaved-PARP increased in a dose-dependent manner. These results indicate that piperine induced apoptosis in AGS cells and may serve as a chemopreventive or therapeutic agent for human gastric cancer.

Contribution of Customer Orientation to Emotional Labor and Customer-Related Social Stressors in School Foodservice Employees -Focus on Daegu and Gyeongbuk Provinces- (학교급식 조리종사원의 고객지향성이 감정노동과 고객관련 스트레스에 미치는 영향 -대구·경북지역을 중심으로-)

  • Lee, Kyung-A;Heo, Chang-Goo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1673-1680
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    • 2016
  • The purpose of this study was to examine how customer orientation (CO) reduces employee stress to customers (CSS). As emotional labor strategies [surface acting (SA) and deep acting (DA)] may mediate a role between CO and CSS, we verified multiple parallel mediator roles of SA and DA between CO and CSS. Our survey was administered to 323 school foodservice employees in Daegu and Gyeongbuk. The results show that CO increased DA but did not decrease SA, and SA had a positive effect on CSS while DA did not influence CSS. These results did not support the parallel multiple mediator model. We conducted post-hoc model modification and proposed the serial multiple mediator model as a modified model. As a result, CO increased DA, DA reduced SA, and SA showed a positive relation with CSS. This double mediating effect through DA and SA between CO and CSS was significant. Based on these results, we found that CO did not have a direct effect on SA and CSS. DA did not directly reduce CSS while CO reduced SA and CSS through DA indirectly. DA had a negative effect on CSS through SA indirectly. Finally, implications and limitations of this are discussed.

DNA Methylation of Multiple Genes in Gastric Cancer: Association with CpG Island Methylator Phenotype and Helicobocter pylori Infection (위암에서 유전자 메틸화와 CpG Island Methylator Phenotype 및 Helicobacter pylori균 감염과의 연관성)

  • Jun, Kyong-Hwa;Won, Yong-Sung;Shin, Eun-Young;Cho, Hyun-Min;Im, Myoung-Goo;Chin, Hyung-Min;Park, Woo-Bae
    • Journal of Gastric Cancer
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    • v.6 no.4
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    • pp.227-236
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    • 2006
  • Purpose: Methylation of gene regulatory elements plays an important role in gene inactivation without genetic alteration. Gastric cancer is one of the tumors that exhibit a high frequency of CpG island hypermethylation. The purpose of this study was to investigate the occurrence of CpG island hypermethylation in gastric carcinoma in relation to H. pylori infection, CIMP and clincopathologic variables. Materials and Methods: We investigated the promoter methylation Status of six genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin) and CIMP in 36 gastric carcinoma tissues as well as in nontumor tissues. CIMP status was investigated by examining the methylation status of MINT 1, 2, 12, 25 and 31. The methylation status of the promoter was examined by methylation-specific PCR (MSP) and H. pylori infection was examined by histological diagnosis after staining with Warthin-Starry silver. Results: Among the 36 gastric carcinoma tissues, DNA hypermethylation was detected in the following frequencies: 14 (38.9%) for p14, 13 (36.1%) for p16, 8 (22.2%) for MGMT, 10 (27.8%) for COX-2, 21 (58.3%) for E-cadherin, and 6 (16.7%) for hMLH1. The frequencies for MINT1 and MINT25 hypermethylation were significantly higher in tumor tissues than in nontumor tissues. 16 (44.4%) of the 36 gastric carcinoma tissues were positive for the CIMP CIMP-H tumors were associated with older patients and larger tumor size than CIMP-L tumors. We found a significant association between the presence of the CIMP and hypermethylation of p16. Hypermethylation of p16 and MINT2 were significantly different when compared by age. MINT1 gene methylation was significantly associated with H. pylori infection (P=0.004). Conclusion: Our results suggest that aberrant hypermethylation of multiple tumor related genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT1, 2, 12, 25, 31) occurs frequently in gastric carcinoma tissues. The hypermethylation of MINT1 was significantly higher in the tumor tissues and was associated with H. pylori infection.

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Clinicopathologic Features and Prognostic Factors for Patients with Large Gastric Tumors (대형 위암의 임상병리학적 분석 및 예후)

  • Jang, You-Jin;Park, Jung-Min;Kim, Jong-Han;Park, Sung-Soo;Kim, Chong-Suk;Mok, Young-Jae
    • Journal of Gastric Cancer
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    • v.6 no.4
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    • pp.244-249
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    • 2006
  • Purpose: Tumor size has been reported to be one of the prognostic factors in the preoperative setting and 8 cm has been confirmed as a cut-off value for large gastric tumors with respect to postoperative complications. The aim of this study was to investigate the clinicopathologic features and the prognosis in patients with tumors larger than 8 cm in diameter. Materials and Methods: We retrospectively studied 2,260 patients with gastric cancer who underwent a gastrectomy from 1983 to 2001 at the Department of Surgery, Korea University College of Medicine. For a comparative analysis we divided the cases into the large and the small groups according to tumor size. The clinicopathological factors associated with large gastric tumors were analyzed by using univariate and multivariate analyses. To determine which variables were independent prognostic factors for overall survival, we applied the Cox proportional hazards model and we used P<0.05 as the cutoff value for statistical significance. Results: Univariate and multivariate analyses disclosed that tumor location (P<0.001), resection type (P<0.001), curability (P<0.001), depth of invasion (P<0.001), number of metastatic lymph nodes (P<0.001), differentiation (P<0.001) and combined resection (P<0.001) were significantly different between the two groups. The independent factors for survival identified by using the Cox proportional hazards model for large gastric tumors were nodal status (P<0.001), curative resection (P<0.001), depth of invasion (P=0.010), type of resection (P=0.018) and age (P=0.033). Conclusion: Large gastric tumors showed more aggressive local findings than their smaller counterparts. In patients with large gastric tumors, a curative resection was the most important factor for the prognosis. Therefore, we suggest that every effort should be made to do a curative gastrectomy and an accurate preoperative examination. (J Korean Gastric Cancer Assoc 2006;6:244-249)

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The Clinicopathological Characteristics of Adenocarcinoma of the Gastro-esophageal Junction (위식도접합부선암의 임상병리학적 특성)

  • Kim, Han-Su;Jeong, Oh;Park, Young-Kyu;Kim, Dong-Yi;Ryu, Seong-Yeop;Kim, Young-Jin
    • Journal of Gastric Cancer
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    • v.8 no.4
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    • pp.210-216
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    • 2008
  • Purpose: Siewert's classification of adenocarcinoma of the esophagogastric junction (AEG) has been widely adopted, but there is a wide discrepancy of the clinicopathological features of AEG of the Asian patients as compared to that of the Western patients. The aim of this study was to investigate the clinicopathological characteristics of AEG according to the Siewert classification. Materials and Methods: Among the patients who underwent surgery for gastric carcinoma in our institution between May 2004 and February 2008, the AEG patients were selected based on their operation records and the photographs according to Siewert's classification. Results: There were 70 AEG patients (3.9%) among the total of 1,778 patients. There were 3 patients (4.3%) with type I, 30 patients (42.8%) with type II and 37 patients (52.8%) with type III. Curative resection (R0) was achieved in 68 cases (97.1%). No significant differences in gender, stage, Barrett's esophagus and the proximal margin were found between the patients with type II and type III AEG. The patients with type III were younger than the patients with type II (59 vs 64 years, respectively, P=0.049). Well differentiated histology (P=0.045) and the intestinal type (P=0.055) were significantly more frequent in the patients with type II as compared with that in the patients with type III. Conclusion: There was a striking difference of the Asian patients from the Western patients for the incidence of AEG (and especially type I). Some of the differences between type II and type III patients were similar to those of the previous Western studies. A large study is needed to investigate whether these features are typical in the Korean population.

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Colon Interposition as a Gastric Substitute after Performing Gastrectomy in Patients with Gastric Cancer (위암 환자에서 위 절제술 후 결장 간치술)

  • Lee, Jun-Hyun;Hur, Hoon;Jeon, Hae-Myung;Kim, Wook
    • Journal of Gastric Cancer
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    • v.8 no.4
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    • pp.217-224
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    • 2008
  • Purpose: For most surgeons, colon interposition after gastrectomy remains an infrequently performed procedure because of its complexity. The aim of this study was to assess its technical feasibility and safety as a post-gastrectomy reconstruction method by reviewing our experience with colon interposition. Materials and Methods: From March 2001 to February 2002, 30 colon interpositions after-gastrectomy were done with using the ileo-ascending or transverse colon. We analyzed the clinicopathologic features and the surgical outcomes. Results: There were 16 males and 14 females in this study with a mean age of 67.5 years (range: 31 to 76 years). Twenty-five ascending colons and 5 transverse colons were used for the interposition, respectively. The mean operation time was 373 minutes (range: 204 to 600 minutes). There were 9 operative morbidities (30%) and 1 operative mortality. The restoration of bowel motility was noted at 3.8 postoperative days; a soft diet was started at 4.9 postoperative days and the duration of the hospital stay was 18.2 days. The percentage of weight loss in the patients with total, proximal and distal gastrectomy was 16.3%, 14.0% and 8.8%, respectively, at 6 months, and thereafter the weight loss gradually recovered as 8.1%, 7.5% and 5.6%, respectively, at 5 years postoperatively. Gastric stasis was the one of the most meaningful long-term complications, and especially in the patients who underwent distal gastrectomy with colon interposition. Conclusion: Colon interposition after gastrectomy was a very complex procedure with a long operating time and many anastomosis sites. The postoperative outcomes failed to achieve satisfactory weight gain and the patients displayed postprandial symptoms. This suggested that this procedure was not an appropriate procedure for conventional reconstruction after gastrectomy.

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