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Effects of Eggshell Powder on Clubroot Disease Control and the Growth of Chinese Cabbage (달걀껍질이 배추의 생육과 무사마귀병 발병억제에 미치는 영향)

  • Kim, Byeong-Kwan;Lim, Tae-Heon;Kim, Youn-Hee;Park, Seok-Hwan;Lee, Sang-Hwa;Cha, Byeong-Jin
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.193-200
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    • 2008
  • Blending of eggshell powder into soil as ratio of 1:5, 1:10, 1:15, 1:20, and 1:25 did not affect seed germination rates of several crops including Chinese cabbage. The blending increased pH of distilled water and decreased the viability of resting spores of Plasmodiophora hrassicae. The ratio of non-viable resting spores in eggshell-blending water was over five times higher than in distilled water of the same pH. Chinese cabbage (cv. 'Norangbom') grew more in eggshell-blended soil than in non-treated soil, but other crops grew less. Leaf numbers and above ground growth of Norangbom increased to around 150% and 470%, respectively, in soil blended with $1:20{\sim}1:15$ of eggshell powder. Even though the optimum sizes of eggshell powder were $0.8{\sim}2.0mm$ for growth and smaller than 0.4 mm for inhibition of clubroot disease of Chinese cabbage, there was no statistical difference among the sizes. Soil pH was above 8.0 in all eggshell treatments without any statistical difference among them. Eggshell powder blending to 1:20 showed lower control efficacy, 58.5%, than registered fungicide 'Hokanna (flusulfamide)', 78.5%. However, Chinese cabbage of that blending ratio recorded the highest growth among the treatments. Therefore, blending of eggshell powder into clubroot-contaminated soil may make culture of Chinese cabbage possible by growth-increasing, even though eggshell powder could not inhibit clubroot disease entirely.

Effect of Cropping System on Disease Incidence by Soil-borne Bymovirus in Barley and on Density of the Vector, Polymyxa graminis (작부형태가 보리의 토양전염성 Bymovirus 발생과 매개균(Polymyxa graminis)의 밀도 변화에 미치는 영향)

  • Park, Jong-Chul;Noh, Tae-Hwan;Kim, Mi-Jung;Lee, Sang-Bok;Park, Chul-Soo;Kang, Chun-Sik;Lee, Jung-Joon;Kim, Tae-Soo
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.115-120
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    • 2010
  • In this study, changes in virus disease occurrence and yield were monitored in conventional cropping system(rice-barley) and soybean-barley double cropping system in virus-prone area for 5 years. Also, changes in the density of Polymyxa graminis, a fungal vector, was investigated. In assay tests, mixed infection of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) was observed. Disease severity was in the range of 7~9 in conventional cropping system. In continuous cropping of soybean-barley and 3-yearfallow land, disease severity also was around 7. However, disease severity was reduced to medium level (5) when barley cultivation was paused for one or two years in soybean-barley cropping. When barley cultivation was paused for a year, the density of P. graminis, a fungal vector for BaYMV and BaMMV, reduced in barley root and soil. Similarly, barley growth was also enhanced by adopting fallow seasons. Compared with the fifth year of conventional cropping, the number of tillers per $m^2$ was increased by 158 when barley cultivation was paused for an year in soybean-barley cropping. When soybean and barley were cultivated continuously or complete fallow period was extended to three years, plant height and the number of tillers of barley were decreased. Yield components of barley in soybean-barley cropping were superior to those in rice-barley cropping. Compared with the fifth year of conventional cropping and soybean-barley cropping, culm length of barley was 1.3~2.3 cm higher and the number of tillers per $m^2$ was 36~90 higher when barley cultivation was paused for one or two years. However, those in continuous cropping of soybean-barley and 3-year-fallow land were lower compared with conventional cropping. Similarly, yield was increased when barley cultivation was paused for one or two years in the third, forth, and fifth years when compared with conventional cropping.

Performance of Laying Period of Two-Way Crossbreed Parent Stock Korean Native Chickens for Producing of Korean Native Commercial Chickens (토종 실용계 생산용 2원 교배종 종계의 산란 능력 검정)

  • Kang, Bo-Seok;Choo, Hyo-Jun;Kim, Hak-Kyu;Kim, Chong-Dae;Heo, Kang-Nyeong;HwangBo, Jong;Suh, Ok-Suk;Choi, Hee-Cheol;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • v.39 no.2
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    • pp.133-141
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    • 2012
  • This study was carried out to investigate the performance of laying period of two-way crossbred of Korean native chickens. A total of four hundred eighty female 2-crossbred chicks that restored strains and were aboriginal at National Institute of Animal Science. Treatments were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ S strain, B) C strain ${\times}$ H strain, C) R strain ${\times}$ S strain, and D) L strain ${\times}$ H strain, respectively. There were no significant difference on age, egg weight, and body weight at first egg among crossbreds (P>0.05). Body weight of D crossbred was lower compared to other crossbreds at the age of 24, 32, 40 and 60 week (P<0.05). Feed intake of A crossbred was highest among crossbreds (P<0.05). Weekly egg weight of A crossbred was the highest among crossbreds all laying period except 48~52 and 60~64 weeks. Hen-house egg production of A and B crossbreds was higher than that of C and D crossbreds (P<0.05). Hen-day egg production decreased after at the age of 36 weeks. Weekly egg production of A and B crossbreds was higher compared to C and D crossbreds (P<0.05). These results suggested the basic data on the record of laying period of 2-crossbreed Korean Native Chickens.

Change of Efficacy and Phytotoxicity of Paddy Herbicide under Temperature Rise (온도상승에 따른 논 제초제의 약효 및 약해 변동)

  • Park, Tae-Sun;Hwang, Jae-Bok;Bae, Hee-Soo;Park, Hong-Kyu;Lee, Gun-Hwi
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.203-211
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    • 2017
  • This study was conducted to investigate the phytotoxicity of main rice varieties and control efficacy of HPPD inhibitor to major paddy weeds at the time of temperature rise due to climate change. Phytotoxicity of herbicide to rice was increased as temperature was increased, and more severe in root than shoot. The phytotoxicity of japonica rice cultivars for the rice were mild enough to recover. However, glutinous rice, super high yield rice, and Tongil rice varieties were damaged enough to decrease the yield. Shindongjinbyeo transplanted by June 15, showed phytotoxicity enough to recover. However, in the rice field on June 30 and on July 15, the rice showed a remarkable inhibition. The control effect of Monochoria vaginalis and Scirpus juncoides was more than 90% under the temperature condition controlled artificially. However, Echinochloa oryzicola was controlled 40% at $27.5^{\circ}C$, which is a high temperature condition. In rice fields with different transplanting times, annual weeds except for E. oryzicola were highly controlled by 90% or more regardless of the time of transplanting.

The Evaluation of Images with Various Filters in I-131 SPECT/CT (I-131 SPECT/CT에서 Ringing Artifact 감소를 위한 다양한 Filter값의 적용)

  • Kim, Ha Gyun;Kim, Soo Mee;Woo, Jae Ryong;Oh, So Won;Lee, Jae Sung;Kim, Yu Kyeong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.18 no.1
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    • pp.62-68
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    • 2014
  • Purpose: After I-131 therapy, SPECT/CT is useful in identifying location of thyroid remnants and metastasis of thyroid cancers. An excessive uptake of thyroid leads to a ringing artifact in the SPECT images. The aim of this study is to investigate and suggest a proper post filters to remove ringing artifact and produce better image quality. Materials and Methods: A low-cost, customized thyroid-mimicking phantom, consisting of an acrylic bottle and a hollow sphere was used for SPECT/CT Discovery (GE Healthcare, USA). It was filled with I-131 solution. The ratio of hollow sphere to background were varied as 50:1, 200:1, 1000:1 and 4000:1. Acquired images were reconstructed by OSEM (2 iterations, 10 subsets) with and without Evolution (resolution recovery correction, GE). Three different post-filters were applied; Butterworth (cut off: 0.38 to 0.58 with intervals of 0.05), Hanning (cut off: 0.8 to 1 with intervals of 0.05) and Gaussian (FWHM: 3 to 5 with intervals of 0.5) filters. Contrast, background variability, air area variability, and full width half maximum (FWHM) were compared. Results: Higher contrasts were obtained from the SPECT images with Evolution than without Evolution. In the case of images without Evolution, image distortion such as star artifact was generated. For all sphere-to-background ratio, the Butterworth filter showed better constrasts and FWHMs than other two filters, but the ringing artifact was still generated in all studies except 50:1 and it was decreased as cutoff value was increased. The ringing artifact didn't appear with Hanning and Gaussian filters at all studies, however constrats and FWHMs with Gaussian was worse than Hanning filter. For the images having ringing artifacts, the background variability and air area variability were increased. Conclusion: In this study, we suggested that it is desirable to use Hanning filter when the ringing artifact is generated and to use Butterworth filter when ringing artifact is not generated in I-131 SPECT.

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Tidal-Flat Sedimentation in a Semienclosed Bay with Erosional Shorelines: Hampyong Bay, West Coast of Korea (해안침식이 우세한 반폐쇄적 조간대의 퇴적작용: 한국 서해안의 함평만)

  • Chang, Jin-Ho;Kim, Yeo-Sang;Cho, Yeong-Gil
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.2
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    • pp.117-126
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    • 1999
  • Hampyong Bay is a semienclosed and macrotidal bay which opens to the eastern Yellow Sea through a narrow inlet in the southwestern coast of Korea. In order to understand the tidal-flat sedimentation in the semienclosed setting, morphology, sediments, accumulation rate and sea cliff erosion were investigated in the tidal flat of Hampyong Bay. The tidal flat of Hampyong Bay lacks intertidal drainage systems, and generally shows the concave-upward profile whose relief is designated by marked morphological features such as high-tide beaches, intertidal sand shoals and tidal creeks. Surfacial sediments of the tidal flat mainly consist of mud, sandy mud, gravelly mud, gravelly sand and muddy gravel, thus showing the textural characteristics of multimodal grain-size distribution, poorly sorting and positive skewness. The sediments generally coarsen landward due to the increase in coarse fraction content. Sedimentary structures are deeply bioturbated, but parallel lamination and lenticular bedding are locally found in the mudflat near mean low water line. Annual accumulation rates across the tidal flat (along Line SM) average -5.2 cm/yr with a range of -45.8~+4.2 cm/yr, indicating that the tidal flat is erosional. In general, erosion rates of upper and lower tidal flat are higher than those of middle tidal flat. Seasonally, the erosion rates are much higher during spring and winter when dominant wind direction corresponds to the long axis of Hampyong Bay. Sea cliffs are eroded at a rate of 1.4 m/yr. The biggest sea cliff erosion generally occurs 1~2 months later after tidal flats were extensively eroded. Such erosions of tidal Oats and sea cliffs in the semienclosed bay setting are interpreted to be due to wind waves coupled with local sea-level rise.

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Introduction and Expression of PAP gene using Agrobacterium in Scrophularia buergeriana Miquel (Agrobacterium을 이용한 PAP 유전자의 현삼으로 도입 및 형질발현)

  • Yu, Chang-Yeon;Seong, Eun-Soo;Lim, Jung-Dae;Huang, Shan-Ai;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.156-165
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    • 2001
  • Exogeneous application of pokeweed antiviral protein (PAP), a ribosomal-inacivating protein in the cell wall of Phytolacca americana (pokeweed) protects heterologous plants from viral and fungal infection. A cDNA clone of PAP introduced into Scrophularia buergeriana Miquel by thransformation with Agrobacterium tumefaciences. For plant transformation, explants were precultured on shoot induction medium without kanamycin for 2-5 day, and then they were cocultured with Agrobacterium for 10 minutes. The explants were placed on co culture medium in dark condition, $28^{\circ}C$ for 2days. After explants were washed in MS liquid medium, they were transferred into selection medium including kanamycin 50mg/L (MS salts+1mg/ l BAP+2mg/ l TDZ+0,2mg/ l NAA+MS vitamin+3% sucrose+0.8% agar, pH5.8). From PCR analysis, NPT II band was confirmed in transgenic plant genome and showed resistance against fungi in antifungal activity test. Micro assay to which protein extracted from transgenic line were added, revealed hyphae growth inhibition and no spore germination at high concentration. The characteristics of inhibited hyphae was represented transparent and thin. Expression of PAP in transgenic plants offers the possibility of developing resistance to viral and fungal infection.

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Presown Seed Treatments to Elevate Seedling Emergence of Codonopsis pilosula $N_{ANNF}$ (만삼의 입묘율 향상을 위한 파종전 종자처리의 모형화)

  • Kang, Jin-Ho;Shim, Young-Do;Jeon, Byong-Sam
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.99-107
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    • 2001
  • Germination of Codonopsis pilosula seeds and afterward their seedling emergence have been occasionally very poor due to their dormancy and structural defects. The study was done to determine the treatment effects of priming, $GA_3$, agitation and washing during and after priming, drying and water imbibition after drying on germination of its seeds and then of the successive three combined treatment after priming on the its seedling emergence to model its presown seed treatment. While agitation at the speed of 10 rpm was made or not, priming using $Ca(NO_3)_2$ and $GA_3$ under their different concentrations and light quality illuminated for 12 hours a day were done separately. Then their two best results were compared to determine the better one. Using the above determined best result, washing was done 3 minutes in the tap water or not, and drying of washed seeds was made under 4 different lights, and water imbibition of the dried seeds with differed period were successively done to deduce its presown seed treatment. In the comparison of the best germination rate of priming and $GA_3$, the former showed considerably higher rate than the latter; the priming treatment was done at agitated $Ca(NO_3)_2$ 150 mM solution under darkness or 12 hour red light illumination a day for 2 days. Washing after priming enhanced the rate compared to no-washing. Drying was the best way at which seeds washed were desiccated under $35^{\circ}C$ and 4 hour red light illumination. Water imbibition immediately before sowing must be made for 2 days. Seedling emergence rate was the highest at the seeds from successive 4 treatments, priming, washing, drying and water imbibition before sowing of two others, the priming and washing, or the priming, washing and water imbibition, meaning that its presown seed treatment must follow the procedure of the above 4 successive ones.

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Studies on the Flowering and Maturity in Sesame (Sesamum indicum L.) II. Capsule-Setting Habit by Different Plant Types (참깨의 개화 등숙에 관한 연구 II. 참깨의 초형에 따른 착삭습성)

  • Jung-Il Lee;;Eung-Ryong Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.4
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    • pp.376-385
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    • 1984
  • The habit of anthesis and maturity of sesame were investigated as a basic research for the improvement of high-yielding varieties and cultural practices. Eight different plant types were identified using typical cultivars among gene pool grown in Korea. Non-branching, Monocapsule, Bicarpels quadriloculi (NMB) type showed higher ratio for capsule setting compared with Non-branching, Monocapsule, Quadricarpels octoloculi (NMQ) type. Non-branching, Tricapsules, Quadricarpels octoloculi(NTQ) type presented lower ratio for capsule setting than non-branching, tricapsules, bicarpels quadriloculi(NTB) type. The number of flowers by flower setting position was the highest on lower part, intermediate on middle part, and the lowest on higher part in NMB and NTB type. However, the number of flowers was the highest on middle part, intermediate on lower part, and lowest on higher part in NMQ and NTQ type. BMB type appeared to have the highest ratio for capsule setting when compared with the other plant types. BTB type with many flowers and capsules exhibited higher percentage for capsule setting than BTQ type with lowest capsule setting percentage. Capsule setting percentage of branch appeared to be lower than that of main stern in the branching type. The branching type had more flowers and capsules than non-branching type. Tricapsules type had more flowers and capsules per plant than monocapsule type, and bicarpels quadriloculi type had more than quadricarpels octoloculi type. However, capsule setting ratio in non-branching type was higher than in branching type. The ratio was higher in monocapsule type than in tricapsules type. Bicarpels quadriloculi type had higher ratio of capsule setting than quadricapels octoloculi type. Number of capsules per plant and capsule setting ratio are supposed to be dominated by shape of capsule (number of carpels and loculi), not by branching or number of capsules per axil. The order of number of capsules per plant was as follows; BTB, BMB, NTB, BTQ, BMQ, NTQ, NMQ, and NMB types. However, the order of capsule setting ratio was as follows: BMB, NMB, NTB, NTQ, NMQ, BMQ, BTB, BTQ types. Consequently, branching, tricapsules, bicarpels quadriloculi(BTB) type was considered as a sourse of breeding for high yielding varieties by introducing the genes govering BMB type with one capsule per axil which has high capsule setting ability.

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Clinical Characeristics of Intracordal Cysts (성대낭종의 임상적 특성)

  • Hong, Ki-Hwan;Park, Jung-Hoon;Kim, Won;Kim, Chang-Hyun
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.10 no.2
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    • pp.164-169
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    • 1999
  • Background and Objectives : The intracordal cysts are more increasingly diagnosed and treated due to advanced laryngeal stroboscopy and laryngeal microsurgical technique. The intracordal cysts are frequently misdiagnosed as vocal polyp or nodule The purpose of this study is to evaluate clinical features of intracordal cysts. Materials and Methods : In the present series, 83 cases of the intracordal cysts treated with laryngeal microsurgery are reported. The intracordal cysts are diagnosed preoperatively with indirect laryngoscopy, laryngeal endoscopy, laryngeal stroboscopy and confirmed with laryngeal microsurgical findings and biopsies. Results : Intracordal cysts are 83 of 1900 patients treated with laryngeal microsurgery(4.4%)-ductal cysts are 56 cases and epidermoid cysts are 27 cases. Intracordal cysts are more frequent in women, forties and the frequent site is an anterior third of the true vocal cord. With the indirect laryngoscopic examination, the ductal cysts are frequently misdiagnosed as vocal polyps or nodules but the epidermoid cysts are relatively easily diagnosed. The etiologic factors of the intracordal cysts are suspected as voice abuse and upper respiratory infection. The degree of postoperative voice satisfaction is similar to that of the vocal polyps. Conclusion : Intracordal cysts are frequently misdiagnosed as polyps or nodules, therefore preoperative stroboscopic findings and laryngeal microsurgical findings is important. An ideal treatment is to enucleate the cysts avoiding rupture of cyst and injury of lamina propria of the vocal cord.

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