• Horticultural Science & Technology
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• v.30 no.5
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• pp.603-612
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• 2012

In order to select potato clones for making cold chip, this study analyzed the glucose content, acrylamide content, and the correlation between the two properties after harvest, $4^{\circ}C$ and $8^{\circ}C$ low-temperature storage, and $20^{\circ}C$ heating treatment of 47 breeding clones and control cultivars 'Atlantic', 'Sumi', and 'Gui Valley'. In all of the control cultivars and 47 clones, glucose content was below 0.25% and acrylamide content was below 1000 ppb just after harvest, but after $4^{\circ}C$ low-temperature storage both the glucose content and acrylamide content increased rapidly and only 4 clones H7, H13, H16, and H40 showed a level below 500 ppb. In $8^{\circ}C$ low-temperature storage as well both contents increased, but the increase was relatively smaller than that in $4^{\circ}C$ low-temperature storage. In addition, $20^{\circ}C$ heating treatment decreased both contents. In the results of analyzing the correlation between glucose content and acrylamide content at low-temperature storage, a positive correlation was observed. In conclusion, clones H7, H13, H16, and H40 showing low glucose content even at low-temperature treatment were found to contain less acrylamide and therefore they were selected as potato clones suitable for making cold chip.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.45-54
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• 2009

This experiment was conducted to clarify the effects of source and sink related characteristics on grain weight increase and grain nitrogen accumulation in rice. Source was modified by leaf removing treatment with no sink modification in 2002 and sink and source were modified at the same time by different sowing dates, N fertilization rates, row spacings, and thinning treatments at heading in 2007. Grain weight (GW) and grain assimilates produced by photosynthesis during grain-filling ($GW_P$) increased with the increase of source, while the grain assimilates retranslocated from leaf and stem ($GW_T$) decreased. Among the source-related characters, shoot dry weight were most closely related with GW. GW was dependant on $GW_P$ rather than on $GW_T$. Grain nitrogen content (GN) and grain nitrogen absorbed from soil during grain-filling ($GN_S$) increased with the increase of source, while the grain nitrogen retranslocated from leaf and stem ($GN_T$) decreased. Shoot nitrogen content among the source-related characters was related most closely with GN. The contribution of $GN_T$ to GN was relatively large although GN depended more largely on $GN_S$ than $GN_T$. In addition, GN was supplied firstly from $GN_S$ or from $GN_S$ and $GN_T$ at the same time.

• Journal of Life Science
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• v.24 no.6
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• pp.618-625
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• 2014

Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, making it less suitable than wheat for many food products such as bread and noodles. The high-molecular weight glutenin subunits (HMW-GS) of wheat play a crucial role in determining the processing properties of the wheat grain. This paper describes the development of marker-free transgenic rice plants expressing a wheat Glu-Dy10 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation. Two expression cassettes, consisting of separate DNA fragments containing Glu-1Dy10 and hygromycin phosphotransferase II (HPTII) resistance genes, were introduced separately into Agrobacterium tumefaciens EHA105 for co-infection. Each EHA105 strain harboring Glu-1Dy10 or HPTII was infected into rice calli at a 3: 1 ratio of Glu-1Bx7 and HPTII. Among 290 hygromycin-resistant $T_0$ plants, we obtained 29 transgenic lines with both the Glu-1Dy10 and HPTII genes inserted into the rice genome. We reconfirmed the integration of the Glu-1Dy10 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the Glu-1Dy10 in transgenic rice seeds were examined by semi-quantitative RT-PCR and Western blot analysis. The marker-free plants containing only the Glu-1Dy10 gene were successfully screened in the $T_1$ generation.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.7-8
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• 1994

한국산 표고버섯 균사체를 액체배양하여 천연항암물질로 알려진 단백다당체를 추출한 후 그 물질의 특성에 대하여 검토하였다. 균사체의 최적 재양조건을 TGY배지로 조사한 바 온도 $25^{\circ}C$, 배양초기 pH4.0, 교반속도 300rpm, 균사배지 접종량을 10.0%로 하고 산소 통기량을 1.0volume of ait/volume of medium/mimute으로 하였을때 가장 양호한 조건이였으며, 대량생산 하기 위한 SCM배지에서 최적의 C/N비는 13.1오써 7일간 배양하였을때 18.8g/L의 균사령을 얻었으며, 이때 생산수율은 0.46으로 나타났다. 발효가 끝난 배양액에서 균사, 여액 그리고 배양액의 전체에서 단백다당체를 분리한 결과 각 분획에서 단백다당체가 각각 0.55%, 0.12%, 0.69%가 회수되어 배양액 전체에서 단백다당체를 추출하는 것이 바람직하며, 추출방법으로 열수추출, glass bead추출 및 cellulasa처리를 하여 단백다당체의 수율을 비교한 결과 0.25-0.5mm glass nead로 30분간 균사체를 분쇄한 다음 열수추출을 1시간을 하였을때 990mg/100ml의 단백다당체를 얻을 수 있었다. 고단백다당체를 1차 단백질 가수분해 효소로 분해하고, EDAE cellulose 및 Sepadex G-100 column chromatography로 정제한 후, TLC/FLD, ultracentrifugation한 결과 순수한 물질임을 알 수 있었다. 단백다당체의 항암효과 조사중 in vitro배양에서 $P_{388}$과 $L_{1210}$에 대한 단백다당체의 활성단위 1 unit는 1mg정도였으며, 인체의 장암세포인 HCT-48, HRT-18, HT-29 밀 간암세포인 Hep G2 대한 생육저해 단위는 각각 4.4, 3.6, 6.6, 2.6mg이었다. HCT-48과 Hep G2 세포의 크기 분포도는 대조군에 비하여 시간이 경과함에 따라, 그리고 단백다당체의 농도가 증가함에 따라 peak가 작은 size 쪽으로 이동하였다. 또, 단백다당체를 첨가 배양한 HCT-48과 Hep G2세포의 현미경 관찰에서 본래의 암세포 형태가 변형되고 크기가 감소하며 세포사이의 경계막이 흐트러지면서 세포수가 감소하고 사멸하였다. In vivo실험에서는 대조군보다 단백다당체를 첨가한 군에서 항체 형성능력이 대조군에 비하여 형질세포가 2배로 증가하였다. 단백다당체의 화학적 성분 분석에서 다당함량은 46.1%이면 구성다당류는 glucose, galactose, mannose, xylose로 구성되었고 단백질의 함량은 7.28%이며, 구성아미노산은 15종의 아미노산으로 되었다. 또 무기물은 Na, K, Zn, Ca등의 순으로 이루어 짐을 알 수 있었다.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.318-321
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• 2011

Rice ageing describes the changes in physical and chemical properties of rice grains during storage that alter the eating quality of rice. The prevention of deterioration in palatability of rice during storage is important for stable supply. In this study, We conducted to select the variety with superior palatability after storage at room temperature. 'Yeonghojinmi' had much higher head rice ratio of milled rice than that of 'Ilmi', 'Junam'and 'Hwayeong'at similar moisture content of approximately 15%. Grossiness and stickiness were higher in 'Yeonghojinmi' than that of the others. Meanwhile, yellowness(b value) of cooked rice was lower in 'Yeonghojinmi' than that of the others. Of the four varieties, Yeonghojinmi was maintained good eating quality after storage in room temperature, which also contributed to differences in palatability, such as protein content, glossiness, stickiness and color of cooked rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.93-103
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• 1992

Experiments were conducted in the research field of the Wheat and Barley Research Institute with three barley cultivars, Olbori, Kangbori and Suwon 18, which showed different growth characteristics. The highest rates of daily dry matter accumulation in grains were 1.03 1.94 mg / grain / day in early and optimum sowings and 0.88 1.88 mg / grain / day in late sowings, which occurred around 20～30 days after heading in early and optimum sowings and 15～20 days after heading in late sowings. Grains reached their maximum weight by 40 days after heading in early and optimum sowings and 35 days after heading in late sowings. Total sugar content in grains followed a pattern of linear increase immediately after heading, but it started to decrease around 20 days after heading. On the other hand, starch content continued to increase until maturity. The contents of both components were high in the order of ＆apos;Olbori＆apos; ＆gt; ＆apos;Kangbori＆apos; ＆gt; ＆apos;Suwon 18＆apos;, but they did not respond in a regular pattern to different sowings. 1,000 grain weight showed highly significant positive correlation(r=0.767$^{**}$) with the duration of grain growth, but it had negative correlations with the average (r=-0.548$^{**}$) or the sum (r=-0.595$^{**}$) of post-anthesis daily mean temperature.ature.

• Journal of the Korean Society of Food Science and Nutrition
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• v.5 no.1
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• pp.19-24
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• 1976

A determination of proper maturity is a significant factor for a good quality and storage. This study is aimed at a determination of optimum harvest date related to a decisive measuring of maturity in Spur Golden Delicious. In this case, respiratory status and other characteristics are taken as a indicator for maturing determination. The results are as follows. 1. Respiratory rate has been decreased through a fruit development and come to its minimized point on Sep. 25. at last. On the contrary its tendency has been increased after Sep. 25. It is also estimated that the former is pre-climacteric, the latter climacteric rise based on dimacteric minimum Sep. 25. 2. A flesh hardness has maintained comparably high value until Sep. 18. But in the point of climacteric minimum, Sep. 25, curve of flesh firmness has rapidly been broken down, and after Sep. 25, tendency has slowly decreased at the sucessive date. 3. Pectin content, optimum acidity are remarkably decreased after Sep. 25, Oct. 2 respectively. 4. When water soluble sugar content shows to be swung from climacteric minimum, any resonable tendency are not found. 5. In this experiment, optimum harvest time is found by Oct. 2, in time of 145 days after flowering.

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.256-262
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• 2017

BACKGROUND: Seed of perilla (Perilla frutescens var. japonica Hara) is short-lived in conventional storage conditions. For long-term conservation of plant species, cryopreservation is the method currently available. This study was performed to find out reliable methods for a long-term storage of seeds of perilla as a genetic resource. METHODS AND RESULTS: Using seeds of 9 perilla cultivars, the effects of desiccation, aging, and cryopreservation on seed germinability and ascorbate peroxidase activity in the seeds were investigated. Initial germinability of the seeds was various, and dry seeds of all cultivars survived cryopreservation without loss of viability. The highest germination was achieved at 4-5% moisture content, and stimulatory effect of cryogenic temperature on the seed germination was observed in some cultivars. Accelerated aging of perilla seeds led to reduction in germination and ascorbate peroxidase activity, and the susceptibility of seeds to aging was different among the tested cultivars. No significant difference in germination was observed for the aged seeds of control and liquid nitrogen exposed. CONCLUSION: The results of this study suggest that cryopreservation at 4-5% moisture content would be a suitable method for long-term conservation of perilla seeds without detrimental effects on germination.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.359-371
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• 2019

To optimize the expression and secretion of ferritin protein associated with ion storage in the mushroom, Pleurotus eryngii, a recombinant secretion vector, harboring the ferritin gene, was constructed using a pPEVPR1b vector under the control of the CaMV 35S promoter and signal sequence of pathogen related protein (PR1b). The ferritin gene was isolated from the T-Fer vector following digestion with EcoRI and HindIII. The gene was then introduced into the pPEVPR1b secretion vector, and it was then named pPEVPR1b-Fer. The recombinant vector was transferred into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformants were selected on MCM medium supplemented with kanamycin and its expression was confirmed by SDS-PAGE and western blotting. Expression of ferritin protein was optimized by modifying the culture conditions such as incubation time and temperature in batch and 20 L airlift type fermenter. The optimal conditions for ferritin production were achieved at 25℃ and after incubating for 8 days on MCM medium. The amount of ferritin protein was 2.4 mg/g mycelia, as measured by a quantitative protein assay. However, the signal sequence of PR1b (32 amino acids) seems to be correctly processed by peptidase and ferritin protein may be targeted in the apoplast region of mycelia, and it might not be secreted in the culture medium. The iron binding activity was confirmed by Perls' staining in a 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in P. eryngii mycelia. Mycelium powder containing ferritin was tested as a feed additive in broilers. The addition of ferritin powder stimulated the growth of young broilers and improved their feed efficiency and production index.

• Applied Biological Chemistry
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• v.38 no.5
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• pp.387-392
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• 1995

To study the regulatory expression mechanism of soybean glycinin gone, Gy2, the 5' upstream region of the gene was searched for the presence of putative regulatory elements by nucleotide sequencing. It revealed various kinds of regulatory sequence elements commonly found in plant storage protein genes. There were canonical promoter sequences, TATA box (TATAAT) and AGGA box (GAAT) which are common in the 5' upstream region of the plant genes. The embryo factor binding sequence, RY repeat, CACA sequences, ${\alpha}$-conglycinin enhancer-like sequences were also found. To delineate the function of these sequences, 5' upstream deletion mutants of Gy2 were prepared and fused to the ${\alpha}$-glucuronidase (GUS) gene. Each chimeric construct was transferred into soybean protoplasts for transient assay, which led to the identification of the sequences between -281 and -223, -170 and -122, of Gy2 promoter as negative regulatory elements, and the sequences between -223 and -170, -122 and -16 as positive regulatory elements. These results are consistent in transformed tobacco plants as well. The serially deleted promoter fragments fused to the GUS were transformed into Nicotiana tabacum by Agrobacterium tumefaciens using the binary vector system. GUS activity of Gy2 promoter deletion constructs was detected only in seeds but not in leaves with different levels of expression as in transient assay. These results suggest that the glycinin Gy2 promoter drives a tissue-specific expression in transgenic tobacco plants.