• KSBB Journal
• /
• v.16 no.6
• /
• pp.626-631
• /
• 2001

Lactobacilli have been considered to play important roles in the health of human vagina. They secrete inhibitory substances to prevent vaginal infection by pathogenic organisms. In a previous study, we have isolated several lactobacilli from Korean woman and one of them (KLB46) was selected and indentified as Lactobacillu crispatus which showed high antimicrobial activity. In this study. cold adaptation prior to subsequent stresses exposure was examined whether L. crispatus KLB46 maintain the viability better than the non-adapted calls under stresses. For pharmaceutical formulation, the lyophilization process is required where stresses such as freezing/thawing and dehydration are routinely applied. Formulated L. crispatus KLB46 can be used for ecological treatment of bacterial vaginosis. The response of cold-adapted cells to other environmental stresses such as acid, heat, ethanol, NaCl, and H$_2$O$_2$ was also examined. The results showed that cold-adapted cells maintained higher survival rate compared with the non-adapted cells (freezing-thawing. 3-folds; dehydration: 3-folds; acid, 3-folds; heat, 10-folds). However, we did net observe any positive effect of cold adaptation on other stresses such as ethanol, NaCl and H$_2$O$_2$. When chloramphenicol was added during cold adaptation, adaptation effect was abolished. This confirms that de novo protein synthesis is necessary during the adaptation process. Moreover, we have identified cold shock protein homolog that codes for a major cold shock protein by PCR amplification using degenerate primers.

• Food Science and Preservation
• /
• v.8 no.1
• /
• pp.30-36
• /
• 2001

Quality changes of winter Chinese cabbage with different packing and loading methods were investigated during cold storage at 0$\^{C}$. Judging from the marketability of stored Chinese cabbage, winter Chinese cabbage could be above 4 months at 0$\^{C}$ cold store. Four Chinese cabbages were packed with 0.3mm polyethylene film and then loaded vertically in plastic container. During storage at 0$\^{C}$, gas composition in the plastic bags was indicated by 8.2∼19.5% O$_2$ and 0.35∼8.58% CO$_2$, respectively. Compared to the conventional storing method, packed with polypropylene net, the fresh1ess of Chinese cabbage was prolonged nearly 10 to 20% by MAP.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.02a
• /
• pp.541-541
• /
• 2013

현재 병원에서 사용하는 치아미백은 고농도의 과산화수소와 carbamide peroxide가 함유된 미백제를 이용하여 전문성을 가진 의사가 직접 시술을 하고 있다 [1]. 과산화수소의 농도가 높을수록 미백효과가 높지만 [2] 과산화수소의 농도가 지나치게 높으면 인체에 유해하다 [3,4]. 따라서, 고농도의 과산화수소가 함유된 미백제의 사용은 전문가만 시술할 수 있도록 이를 제한하고 있다. 이로 인해, 일반 가정용으로 판매되는 다양한 미백제품들은 저농도(최대6%)의 과산화수소가 함유되어 있으며 장기간 지속적으로 사용해야만 치아미백효과를 볼 수 있다. 우리는 가정에서도 보다 안전하고 단기간에 효율적으로 치아의 미백효과를 보기 위하여, 식품의약품안정청에서 규제하고 있는, carbamide peroxide (15%)와 저온 대기압 플라즈마 제트를 사용하여 미백효과를 관찰하였다. 플라즈마 제트의 유량은 200 sccm이며, 공기를 사용하였다. 미백효과를 보기 위한 대상으로는 우치(牛齒)를 사용하였으며, 플라즈마를 처리하여 미백효과를 관찰하였다. 실험 대조시료군으로는 carbamide peroxide (15%)를 처리하지 않은 우치와 처리한 우치, 그리고 carbamide peroxide (15%)를 처리한 우치에 수증기(0.2~1%)를 첨가한 다음, 이들 세가지 시료에 각각 공기 플라즈마를 조사하여 비교해보았다. 모든 실험의 플라즈마 처리시간은 최대 20분까지로 하였다. 수증기를 첨가한 이유는 활성산소의 농도를 높이기 위함이며, 이로써 탁월한 미백효과를 얻을 수 있다. 이는 활성산소와 치아의 유기질이 반응하여, 색이 진한 탄소고리 화합물을 밝은 색의 사슬구조로 바꿔주기 때문이다. 실험을 통하여 우치에 carbamide peroxide (15%)와 수증기(0.2~1%)를 처리한 경우 플라즈마의 미백효과가 탁월함을 보였다. 이때 CIE색좌표 ($L^*a^*b^*$)에서 명도도가 최대 2배 이상 높아짐을 보았다. 미백효과에 대한 측정은 측색분광기(CM-3500d)를 이용하였다.

• Food Science and Preservation
• /
• v.18 no.2
• /
• pp.143-148
• /
• 2011

This study was conducted to investigate the effect of CA(4% $O_2$ and 7.5% $CO_2$) storage on the quality characteristics and chilling injury in 'Nanko' prunus mume fruits at 1, 5, and $9^{\circ}C$. CA storage reduced production of $CO_2$ and $C_2H_4$ significantly. Hue values of fruit skin were significantly higher in fruits stored at $1^{\circ}C$ and $5^{\circ}C$ than $9^{\circ}C$. Weight loss was much lower in fruits stored under CA storage. Soluble solids content (SSC) titratable acids (TA), and firmness were maintained and electrolyte leakage was lower in fruits stored under CA storage. Ratios of chilling injury and decay were increased faster at $5^{\circ}C$ and $9^{\circ}C$ than $1^{\circ}C$. The chilling injury was suppressed in fruits of CA storage compared with control fruits during cold storage. These results indicate that CA storage at $1^{\circ}C$ of prunus mume fruits extended the storage life up to 30 days without quality deterioration. effectively.

• Korean Journal of Agricultural Science
• /
• v.27 no.2
• /
• pp.86-94
• /
• 2000

This study was carried out to determine the effectiveness of uniconazole in ameliorating low and high temperature injury in tomato plants(Lycopersicon esculentum Mill. cvs. Fireball and Patio). Plants were given a soil drench of 0, 0.001, 0.01 or $0.1mg{\cdot}pot^{-1}$ uniconazole, and after 14 days, were treated with 12-h day/12-h night cycles at $25/25^{\circ}C$, $2.5/25^{\circ}C$, $25/2.5^{\circ}C$ or $40/40^{\circ}C$ for 4 days in controlled-environment chamber. Number of damaged leaves per plant, reduction of stem elongation, and overall injury were high at $2.5/25^{\circ}C$, but more reduction of leaf elongation, delay of flowering, and abortion of floral bud were observed in plants at $40/40^{\circ}C$. There was difference in degree of injury between cultivars, thus, 'Fireball' was much affected by unfavorable temperature regimes. All concentrations of uniconazole reduced leaf and stem elongation, increased total chlorophyll concentration, delayed flowering, and significantly provided protection against low and high temperature injury in two cultivars. In general, the application of uniconazole did not inhibit flowering delay and floral bud abortion induced by high and low temperature exposure. Our results support the hypothesis that the role of uniconazole is related to defense system against oxidative stress induced by low temperature stress. Further research is required to clarifu the phytoprotective mechanism of this compound agaist high temperature stress.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 1994.11a
• /
• pp.35-36
• /
• 1994

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2001.11a
• /
• pp.83-88
• /
• 2001

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2002.11a
• /
• pp.25-25
• /
• 2002

• Journal of agriculture & life science
• /
• v.43 no.5
• /
• pp.43-46
• /
• 2009

The one of problems of plug seedlings production in winter season was a poor growth. This study was carried out to investigate the effects of treatments with yeast fertilizers on the growth of pepper seedlings in winter seasons. The plant height, internode length, and shoot and dry weight of pepper seedlings increased significantly by yeast fertilizers. The results showed that yeast fertilizer treatment could be more effective in soaking than foliar spray and that of dilution could be effective in $1,000{\times}$.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.11 no.1
• /
• pp.55-60
• /
• 2010

Vortex tube is the device that can separate small particles from the compressed gas, as well as compressed gas into hot and cold flow. Due to energy separation ability, a vortex tube can substitute for an EGR cooler of the automotive engine. In this study, experimental approach has been performed to analyze the energy separation characteristics of the vortex tube. Energy separation characteristics of the vortex tube has been tested for supply pressure, cold-out pressure, and hot-out pressure. As increasing supply pressure, energy separation effect increased. Maximum temperature exists about 0.85 of the cold-out-flow-ratio, and minimum exists about 0.35. Hot-out temperature of the vortex tube is affected by the hot-out and cold-out pressure. However, for the given conditions, cold-out temperature is independent of exit pressure change. The results from this study can be used for the basic design parameter of the EGR cooler substitute of an automotive engine.