• Title/Summary/Keyword: 저온상

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Comparative Study of Prolonged Preservation Methods in Rabbit Heart for Transplantation (이식을 위한 가토심장의 장기 보존방법에 관한 비교 연구)

  • Jo, Hyeong-Gon;Kim, Su-Hyeon;Kim, Song-Myeong
    • Journal of Chest Surgery
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    • v.30 no.1
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    • pp.1-10
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    • 1997
  • The successful cardiac transplantation depends partly on the donor heart preservation by a solution that will ensure recovery of myocardial function. The purpose of this study was to perform the evaluation of various preservation solutions and to accumulate the data on the requisites for ideal preservation solution. The experimental setup was the constant pressure Langendorffs perfusion system. Isolated rabbit hearts were perfused for 20minutes with unarm Krebs-Henseleit solution, stored for 4 hours in cold preservation solution after cardioplegia, and then were reperfused for 20minutes. The 4 experimental groups were prepared Hartmann's solution group (group 1, control), modified Euro-collins solution group(group II. MEC), modified University of Wisconsin group (group n, MUW), and CK solution(made by the author) group (group W, CK). The parameters for assessing the preservation ability were levels of enzymes in freezed myocardial tissues (lactate, creatine kinase-MB and adenosine deaminase), coronary flow. left ventricular developing pressure and dpldt. In conclusion, the ability of preservation for isolated rabbit heart was excellent in CK solution and modified University of Wisconsin solution, and poor in modified Euro-collins solution, compared with Hartmann solution. CK solution has low potassium concentrations(34.2mEq/L) and includes various substrates to be salutary on myocardial preservation. This fact may indicates the necessity of further refinements in selection or composition of electrolytes and substrates.

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Processing of Low Salt Mackerel Fillet and Quality Changes during Storage (저염 고등어 Fillet의 제조 및 저장중 품질변화)

  • Lee, Kang-Ho;Hong, Byeong-Il;Jung, Byung-Chun
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1070-1076
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    • 1998
  • The processing conditions of low salt mackerel (Scomber japonicus) fillet was investigated, in which fresh mackerel was filleted, salted in brine until the expected salt concentration reached, dried with cool air (3 m/sec, $10{\sim}20^{\circ}C$), and finally packed individually in polyvinyl chloride film. Salting time and salt concentration of brine decided the final salt level penetrated into the fillet. As the final salt level was fixed to $0.8{\sim}1.0%, salting for $15{\sim}20 hours with 5% or 10% brine at $5^{\circ}C$ was enough to get that level of salt. Formation of histamine during salting was negligible. Changes in VBN, salt soluble proteins, and histamine formation of salted mackerel fillet during the storage occurred more rapidly in cases of storage at $5^{\circ}C than af $-2^{\circ}C and $-20^{\circ}C. Oxidation of lipid during the storage progressed, however it was delayed longer then 100 days in case of storage at $-20^{\circ}C. Addition of sodium erythrobate or ginger extracts could provide some extent of browning retardation. The shelf-life of the salted mackerel fillet based on panel scores of brown color and rancidity appealed to be 14 days when stored at $5^{\circ}C, and more than 28 days in case of storage at $-2^{\circ}C and about 3 months stored at $-20^{\circ}C.

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Microtuber Formation from In Vitro Codonopsis lanceolata Plantlets by Sugar (탄소급원처리에 의한 기내 더덕 식물체의 비대근 형성)

  • Kim, Ji-Ah;Moon, Heung-Kyu;Choi, Yong-Eui
    • Journal of Plant Biotechnology
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    • v.40 no.3
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    • pp.147-155
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    • 2013
  • In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0 $mg{\cdot}L^{-1}$ of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at $4^{\circ}C$ refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.

Poly-Si MFM (Multi-Functional-Memory) with Channel Recessed Structure

  • Park, Jin-Gwon;Jo, Won-Ju
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.156-157
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    • 2012
  • 단일 셀에서 비휘발성 및 고속의 휘발성 메모리를 모두 구동할 수 있는 다기능 메모리는 모바일 기기 및 embedded 장치의 폭발적인 성장에 있어 그 중요성이 커지고 있다. 따라서 최근 이러한 fusion기술을 응용한 unified RAM (URAM)과 같은 다기능 메모리의 연구가 주목 받고 있다. 이러한 다목적 메모리는 주로 silicon on insulator (SOI)기반의 1T-DRAM과 SONOS기술 기반의 비휘발성 메모리의 조합으로 이루어진다. 하지만 이런 다기능 메모리는 주로 단결정기반의 SOI wafer 위에서 구현되기 때문에 값이 비싸고 사용범위도 제한되어 있다. 따라서 이러한 다기능메모리를 다결정 실리콘을 이용하여 제작한다면 기판에 자유롭게 메모리 적용이 가능하고 추후 3차원 적층형 소자의 구현도 가능하기 때문에 다결정실리콘 기반의 메모리 구현은 필수적이라고 할 수 있겠다. 본 연구에서는 다결정실리콘을 이용한 channel recessed구조의 다기능메모리를 제작하였으며 각 1T-DRAM 및 NVM동작에 따른 memory 특성을 살펴보았다. 실험에 사용된 기판은 상부 비정질실리콘 100 nm, 매몰산화층 200 nm의 SOI구조의 기판을 이용하였으며 고상결정화 방법을 이용하여 $600^{\circ}C$ 24시간 열처리를 통해 결정화 시켰다. N+ poly Si을 이용하여 source/drain을 제작하였으며 RIE시스템을 이용하여 recessed channel을 형성하였다. 상부 ONO게이트 절연막은 rf sputter를 이용하여 각각 5/10/5 nm 증착하였다. $950^{\circ}C$ N2/O2 분위기에서 30초간 급속열처리를 진행하여 source/drain을 활성화 하였다. 계면상태 개선을 위해 $450^{\circ}C$ 2% H2/N2 분위기에서 30분간 열처리를 진행하였다. 제작된 Poly Si MFM에서 2.3V, 350mV/dec의 문턱전압과 subthreshold swing을 확인할 수 있었다. Nonvolatile memory mode는 FN tunneling, high-speed 1T-DRAM mode에서는 impact ionization을 이용하여 쓰기/소거 작업을 실시하였다. NVM 모드의 경우 약 2V의 memory window를 확보할 수 있었으며 $85^{\circ}C$에서의 retention 측정시에도 10년 후 약 0.9V의 memory window를 확보할 수 있었다. 1T-DRAM 모드의 경우에는 약 $30{\mu}s$의 retention과 $5{\mu}A$의 sensing margin을 확보할 수 있었다. 차후 engineered tunnel barrier기술이나 엑시머레이저를 이용한 결정화 방법을 적용한다면 device의 특성향상을 기대할 수 있을 것이다. 본 논문에서는 다결정실리콘을 이용한 다기능메모리를 제작 및 메모리 특성을 평가하였다. 제작된 소자의 단일 셀 내에서 NVM동작과 1T-DRAM동작이 모두 가능한 것을 확인할 수 있었다. 다결정실리콘의 특성상 단결정 SOI기반의 다기능 메모리에 비해 낮은 특성을 보여주었으나 이는 결정화방법, high-k절연막 적용 및 engineered tunnel barrier를 적용함으로써 해결 가능하다고 생각된다. 또한 sputter를 이용하여 저온증착된 O/N/O layer에서의 P/E특성을 확인함으로써 glass위에서의 MFM구현의 가능성도 확인할 수 있었으며, 차후 system on panel (SOP)적용도 가능할 것이라고 생각된다.

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Mineralogical Characteristics of Tachylite occurring in Basic Dike, Basaltic Agglomerate Formation, Ulleung Island and Its Implications of Volcanic Activity (울릉도의 하부층 현무암질 집괴암 층내 염기성 암맥에서 산출되는 타킬라이트의 광물학적 특성과 화산학적 의미)

  • Bae, Su-Gyeong;Choo, Chang-Oh;Jang, Yun-Deuk
    • Journal of the Mineralogical Society of Korea
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    • v.25 no.2
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    • pp.63-76
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    • 2012
  • Tachylite, black basaltic glass formed by the rapid cooling of molten basalt, locally occurs at the Basaltic Agglomerate Formation (BAF), the lowest formation of Ulleung Island. The purposes of this study are to characterize the occurrence and mineralogy of tachylite and to elucidate its formation condition, with emphasis on its fracture pattern, which can serve as implications for the early volcanic activity of Ulleung Island. To this end, we investigated the occurrence pattern of tachylite in the field and carried out mineralogical analyses using optical microscope, XRD, EPMA, and SEM. Tachylite occurs at the chilled margin of basic dikes which are distributed in Naesujeon, Dodong and Jeodong seasides, Turtle Rock, and Yaerimwon, whose widths vary from several cm to 10 cm. It is evident that the outer surface of tachylite is dense and smooth, whereas the inner surface, if fractured, is characterized by conchoidal fracture. The matrix of tachylite consists of amorphous, glass and some fine-grained phenocrysts present in tachylite include biotite, anorthoclase, sanidine, plagioclase, hornblende, and Fe-Ti oxides. The fracture patterns characteristic of tachylite are subrounded, oval, or less commonly polygonal, bounded by joints to form globule or lump. Taking into account texture and mineralogy, tachylite is interpreted to have undergone little subsequent alteration at low temperature via hydration or hydrolysis that could form clay minerals after it was formed. Because tachylite with peculiar fractures occurs as dikes in a close association with BAF, its presence is considered as reliable evidence that when tachylite formed, the most part of BAF was still under subaqueous conditions, or at least saturated with seawater.

Growth and Dry Matter Accumulation in Different Ecotypes of Soybeans (Glycine max. L.) (생태형이 다른 대두품종의 생육 및 건물축적)

  • 정길웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.174-181
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    • 1988
  • A comparison was made the differences of growth and some yield components of two soybean ecotypes (summer and two autumn types) at different planting dates (15 April, 15 May, and 15 June) and also made the different patterns of dry matter accumulation of one summer type and two autumn types of soybeans at Agricultural Experiment Farm, College of Agriculture, Dankook University, Cheonan City, in 1987. [Planting date experiment] Days to emergence of four varieties were observed no difference. and, therefore, it was speculated that field observation of the difference of cold tolerence between varieties was difficult. Shortening rate of flowering clays as planting dates delayed was no different between two ecotypes, summer vs. autumn types of soybean. Summer types, DN82029-3 and ES18085-1, were observed not greater difference of days to maturity between early and late planting dates as compared with that of autumn types, Changyeobkong and Paldalkong. At late planting, summer types were observed more to hasten days to ripening due to high temperature in late growing season than that of autumn types. This caused to decrease somewhat greater rate of some yield componments, and finally yield as planting date delayed. As compared to autumn types, summer types, DN82029-3 and ES18085-1 showed longer stem height and lower airdry weight at late planting. [Dry matter accumulation pattern] Stem elongation after flowering of three varieties, DN82029-3, Changyeobkong and Danyeobkong, ranged 17 to 32 cm and increment of leaf area index 0.83-1.53. DN82029-3 reached 49 days faster in maximum total dry weight than that of autumn types, Changyeobkong and Danyeobkong. However. maximum total dry weight of DN82029-3 showed 50% to autumn types. At 15 May planting, summer type, day-neutrality, transfered faster into reproductive growth phase without enough growth of vegetative growth and also hastened ripening days, and thus lower dry matter accumulation and finally lower yield.

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Highly Dispersed Supported Gold Catalysts -I. Effect of Gold Addition and Active Site Formation- (고분산 담지 금촉매 - I. 금의 첨가 효과 및 활성점 생성 -)

  • Ahn, Ho-Geun;Niiyama, Hiroo
    • Applied Chemistry for Engineering
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    • v.5 no.2
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    • pp.285-294
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    • 1994
  • Some supported gold catalysts were prepared by impregnation and coprecipitation methods. Effect of gold addition and active sloe formation were studied by investigating particle sizes of gold, amounts of oxygen adsorbed, adsorption properties of CO and NO, and reduction and oxidation properties, etc.. The gold particles of the catalyst by impregnation were irregular and very large as 30~100 nm, but those by coprecipitation were uniform and ultra-fine as about 4 nm. On $Au/Al_2O_3$ catalyst, the addition of gold to inactive $Al_2O_3$ caused the decomposition of $N_2O$, and CO was not irreversibly adsorbed while $O_2$ was atomically and irreversibly adsorbed. The adsorption sites of oxygen were attributed to the active sites which were restricted to the circumference of hemispherical gold particle-support interface rather than all atoms on the surface of gold particle. Also, CO was reversibly and irreversibly adsorbed on $Al_2O_3$ at low temperature, and the addition of gold weakened both reversible and irreversible adsorptions. The affinity for CO on $Au/Co_3O_4$ catalyst decreased conspicuously compared to $Co_3O_4$. The effect of gold addition did not appear in reduction step but did remarkably in reoxidation step; the added gold promoted the reoxidation of the reduced cobalt atoms.

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Fabrication and Photocatalytic Properties of SiO2-TiO2 Composite Nanofibers (SiO2-TiO2계 복합 나노섬유의 제조 및 광활성 연구)

  • Hyun, Dong Ho;Lim, Tae-Ho;Lee, Sung Wook
    • Applied Chemistry for Engineering
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    • v.19 no.5
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    • pp.554-558
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    • 2008
  • $(1-x)SiO_2-(x)TiO_2$ composite fibers with various compositions of $TiO_2$ were prepared by electrospinning their sol-gel precursors of titanium (IV) iso-propoxide (TiP), and tetraethyl orthosilicate (TEOS). The surface morphology and structure of sintered composite fibers were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), simultaneous thermogravimetric analysis-differential scanning calorimetry (TGA-DSC) and Fourier transform infrared spectroscopy (FT-IR). As the content of $TiO_2$ in $(1-x)SiO_2-(x)TiO_2$ system was increased the average diameter of composite fibers was proportionally increased. Also, the transformation of $TiO_2$ from anatase to rutile form was inhibited by the highly dispersed $TiO_2$ around $SiO_2$ particles up to $0.6SiO_2-0.4TiO_2$ composite fibers even after calcination at $1000^{\circ}C$. The photocatalytic activity of $SiO_2-TiO_2$ composite fibers was examined for the methylene blue (MB) decomposition which was confirmed using UV-vis/DRS spectra. The experiments demonstrated that the MB in aqueous solution was successfully photodegraded using $SiO_2-TiO_2$ composite nanofibers under UV-visible light irradiation.

The Effect by Aqueous NH4OH Treatment on Ru Promoted Nickel Catalysts for Methane Steam Reforming (암모니아 용액 처리에 의한 Ru-Ni/Al2O3 촉매의 메탄 수증기 개질 반응에 미치는 영향)

  • Lee, Jung Won;Jeong, Jin Hyeok;Seo, Dong Joo;Seo, Yu Taek;Seo, Yong Seog;Yoon, Wang Lai
    • Applied Chemistry for Engineering
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    • v.17 no.1
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    • pp.87-92
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    • 2006
  • The steam reforming of methane over Ru-promoted $Ni/Al_2O_3$ was carried out. Compared with $Ni/Al_2O_3$, which needs pre-reduction by $H_2$, $Ru/Ni/Al_2O_3$ catalysts exhibited relatively higher activity than conventional $Ni/Al_2O_3$. According to $H_2-TPR$ of reduced or used catalysts and $CH_4-TPR$, it was revealed that the reduction of $RuO_x$ by $CH_4$ decomposition begins at a lower temperature ($220^{\circ}C$) and the reduced Ru facilitates the reduction of NiO, and leads to self-activation. To improve metal dispersion, the catalyst was soaked in 7 M aqueous $NH_4OH$ for 2 h at $45^{\circ}C$ while stirring. As a result, $Ru/Ni/Al_2O_3$ catalysts with aqueous $NH_4OH$ treatment have higher activity, larger metal surface area (by $H_2$-chemisorption), and small particle size (by XRD and XPS). It is noted that the amount of noble metal could be reduced by aqueous $NH_4OH$ treatment.

Cell Entrapment for Bifidobacteria to Increase Viability and Preservative Stability using Erythritol (Erythritol을 이용한 Bifidobacteria의 생존력과 저장안정성 증대를 위한 세포포집)

  • 임태빈;백인걸;정찬섭;류지성;지근억;허병기;허태련
    • KSBB Journal
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    • v.17 no.6
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    • pp.531-536
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    • 2002
  • In this study, we attempted to increase the survivability of bifidobacteria in simulated gastric juices and bile salts after cell entrapment with alginate and various food additives, such as erythritol, isomalt, palatinose, skim milk, xanthan gum, isomalto-oligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, pectin, and mono-sodium glutamate. Additionaly, the stability of bifidobacteria during storage was investigated by measuring survival rate at different temperatures, i.e. at 4$^{\circ}C$, 25$^{\circ}C$ and -20$^{\circ}C$. Bifidobacteria were immobilized in alginate beads and the survival rate was monitored. It was found that bifidobacieria entrapped with 2.5%, alginate showed the highest survival rate at 12%. After addition of the various protective agents, erythritol(1%) showed the best protective efficiency with a survival rate of 56.0% among the additives tested when exposed to simulated gastric juices for 3 h. Immobilized cells suspended in 5% skim milk and stored at 4$^{\circ}C$ survived significantly more than cells stored at 25$^{\circ}C$ and -20$^{\circ}C$. Consequently, the study shows that the survival rate of bifidobacteria immobilized in combination with 2.5% alginate beads and 1% erythritol may be signifcantly increased in simulated gastric juices and bile salts.