• 보존과학연구
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• s.15
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• pp.59-103
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• 1994

Painting is well regarded as a stratified structure by the conservators and restorers. Hence, the scientific methods have been developed for the study of the interal layer of paintings. Examples of such methods are X-ray, infra-red, and ultra-violet photography. A more direct method is to look at the painting in cross section under the microscope and to analyze pigments using an electron probe X-ray micro analyzer(EPMA).In this research, I study and analyze twenty two Korean paintings of self-portraits including the first oil painting of Hui Dong Koh's self-portrait stored in the museum of Tokyo National University of Fine Arts and Music, employing these scientific techniques. The small fragments taken from the ground layers of the early oil paintings(1915∼1942)are analyzed using the EPMA. According to their main materials, the ground layers can be classified into five types ; 1. Lead white layer and double layer of calcium carbonate and lead white, 2. Zinc white with some mixiture of lead white, 3.Titanium white with some barium white, 4. Barium white, 5.Double layer of titanium white and zinc white.

• Journal of Korean Medical classics
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• v.21 no.1
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• pp.303-315
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• 2008

안등창익(安藤昌益)(1703-1762)시일본강호시대적의사(是日本江戶時代的醫師), 야시독창이진보적사상가(也是獨創而進步的思想家). 비시의학적시대(卑視醫學的時代), 타주장여의론병제고의술혹의도적사회지위(他主張與醫論병提高醫術惑醫道的社會地位). 타사료(他寫了)"자연진영도(自然眞營道)"전정료대어의학원리화치방기술상참신적철학기초. 타적의학사상불시종병론출발(他的醫學思想不是從病論出發), 이시종생명론출발적(而是從生命論出發的), 즉생명시자연적창조물(卽生命是自然的創造物). 타인위유어사회적모순(他因爲由於社會的矛盾), 대자연화인회생병(大自然和人會生病), 나착사회병리학적기초(拿着社會病理學的基礎), 사병인론체계화(使病因論體系化). 이차병인시제기관관계적고취(而且病因是諸器官關係的告吹), 호성적부조(互性的不調), 인차타나출농정호성관계적호성치료론(因此他拿出弄正互性關係的互性治療論). 창익부정종장부론도삼초화심포적존재(昌益否定從藏腑論到三焦和心包的存在), 병중시위화비특이적기능. 인이주장별유분개적사장사부론(因而主張別有分開的四臟四腑論). 창익강조자연치유법적의학사상(昌益强調自然治癒法的醫學思想), 중시예방의학(重視豫防醫學), 재치료상현현출소극적일면(在治療上顯現出消極的一面). 재가상비판전통적의학론(再加上批判傳統的醫學論). 타이독창적자연철학위기초(他以獨創的自然哲學爲基礎), 당확립신적의학방법론시(當確立新的醫學方法論時), 사호범료조지과급적착오(似乎犯了燥之過急的錯誤). 상차여차(尙且如此), 타적독창사상도현재부단몰유피망기(他的獨創思想到現在不但沒有被忘記), 다소재의학분야상유열렬적후계자화지지자시(多少在醫學分野上有熱烈的後繼者和支持者是), 아문응해요주목적일면(我們應該要注目的一面).

• Journal of Conservation Science
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• v.16 s.16
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• pp.89-103
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• 2004

Provenance discrimination of sixty four obsidian artifacts found in the Korean Peninsular has been carried out using major elements, minor elements and magnetic properties. Most of obsidians in this study were classified into the subalkaline series based on the contents of $Al_2O_3,\;CaO,\;and\;Na_2O+K_2O$ in samples. Provenance & obsidians were classified into three major groups using major elements and minor elements analyse. This result suggests that there are no correlations among the three groups whose provenances are different. Although the magnetic properties of obsidians do not yield clear groupings according to the archaeological site, the bivariate plot for the magnetic susceptibility and the saturation isothermal remanent magnetization shows a rough correlation with the results of major and minor elements analyses.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.17-25
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• 2013

The thermotolerant methylotrophic yeast Hansenula polymorpha is an attractive model organism for various fundamental studies, such as the genetic control of enzymes involved in methanol metabolism, peroxisome biogenesis, nitrate assimilation, and resistance to heavy metals and oxidative stresses. In addition, H. polymorpha has been highlighted as a promising recombinant protein expression host, especially due to the availability of strong and tightly regulatable promoters. In this study, we investigated the possibility of employing human serum albumin (HSA) as the fusion tag for the secretory expression of heterologous proteins in H. polymorpha. A set of four expression cassettes, which contained the methanol oxidase (MOX) promoter, translational HSA fusion tag, and the terminator of MOX, were constructed. The expression cassettes were also designed to contain sequences for accessory elements including His8-tag, $2{\times}(Gly_4Ser_1)$ linkers, tobacco etch virus protease recognition sites (Tev), multi-cloning sites, and strep-tags. To determine the effects of the size of the HSA fusion tag on the secretory expression of the target protein, each cassette contained the HSA gene fragment truncated at a specific position based on its domain structure. By using the Green fluorescence protein gene as the reporter, the properties of each expression cassette were compared in various conditions. Our results suggest that the translational HSA fusion tag is an efficient tool for the secretory expression of recombinant proteins in H. polymorpha.

• Korean Journal of Heritage: History & Science
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• v.46 no.3
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• pp.48-67
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• 2013

This study was focused on the recognition of historical values and the establishment of conservation schemes for a Silla stone monument in Bongpyeong-ri, Uljin by combining the humanistic investigation of inscription reinterpretation and the scientific conservational diagnosis of deterioration. According to the investigation of inscription, a total of 13 letters were reconsidered compared to the preceding researches. Thus, the meaning and interpretation of previous inscription was partially changed. This monument is composed of gneissose leucogranite and the most suitable site as provenance of the stone would be the Jukbyeon seashore (2.1km). The site shows similar color, size and composition of minerals, gneissose structure and magnetic susceptibility as the Bongpyeong stone monument. This monument developed a structural crack (crack index 0.4) and a microcrack (crack index 2.0) along the gneissose structure. The horizontal strength is weaker than the vertical strength. Therefore, the cracks should be reinforced and treated. However, consolidating is not urgently needed because the total weathering grade by ultrasonic velocity shows the stage of moderate weathering(3,403m/s, 0.32). Also, the major problems of chemical deterioration are blackening (85.2%) with soil, iron oxide,rubbing mark, and salt crystals (17.3%) from the sea. Therefore, the contaminants and the salt crystals should be removed using pressure spray and pulp paper, while the application of poultice should be examined through clinical tests.

• Composites Research
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• v.36 no.5
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• pp.289-296
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• 2023

In this study, we investigated the electromagnetic properties and microwave absorption characteristics of M-type hexagonal ferrites, which are known as millimeter-wave absorbing materials, according to their calcination temperature. The M-type ferrites synthesized using a molten salt-based sol-gel method exhibited a single-phase M-type crystal structure at calcination temperatures above 850℃. The synthesized particle size increased as well with the calcination temperature. Saturation magnetization increased gradually with increasing calcination temperature, but coercivity reached a maximum at 1050℃ and then rapidly decreased. After preparing a thermoplastic polyurethane (TPU) composite containing 70 wt% of M-type ferrites, we measured the complex permittivity and permeability in the Q-band (33-50 GHz) and V-band (50-75 GHz) frequency ranges, where ferromagnetic resonance occurred. Strong magnetic loss from ferromagnetic resonance occurred in the 50 GHz band for all composite samples. Based on the measured results, we calculated the reflection loss of the TPU/M-type ferrite composite. By calculating the reflection loss of the M-type ferrite composite, the M-type ferrite calcined at 1250℃ showed excellent electromagnetic wave absorption performance of more than -20 dB at 52 GHz with a thickness of about 0.5 mm.

• Journal of Conservation Science
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• v.28 no.3
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• pp.185-192
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• 2012

Issues of chronology on archaeological remains or relics have been a storm-center of controversy when various archaeological researches have been done. Sometimes there is a limit for figuring out issues of chronology by archaeological research. In that case, the field of natural science is often needed to work out issues of chronology. Among various subjects in natural science, archaeomagnetism plays an important role in dating archaeological remains for baked earth bearing relics. In particular, archaeomagnetism is of use for sites where directly excavated dating proxy is unavailable. Terrestrial magnetism changes along with the passage of time and leaves trace by many kinds of residual magnetization which could be called fossil of terrestrial magnetism. Archaeomagnetic dating method is used to assign a date to the archaeological remains in which baked earth is found by measuring the changes of terrestrial magnetism through the thermal remanent magnetization retained in baked earth. This study aims to constrain the age of fire at Buinsa, Daege, Korea using 27 samples that were collected from a layer of baked earth. Buinsa is famous for the place where kept the first edition of Tripitaka Koreana, which was lost in fire at the second invasion of mongolia. In addition, there is a record that there was revolt around this region in A.D.1203. According to archaeomagnetic dating, ages of A.D.1150~1200 and A.D.1130~1210 were assigned for the two building sites in Buinsa, respectively. To this end, it can be concluded that the layer of baked earth on the two building sites in Buinsa recorded the vestige of fire caused by revolt.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.7
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• pp.770-778
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• 2023

Sea surface wind is an important variable for elucidating the atmospheric-ocean interactions and predicting the dangerous weather conditions caused by oceans. Accurate sea surface wind data are required for making correct predictions; however, there are limited observational datasets for oceans. Therefore, this study aimed to obtain long-period high-resolution sea surface wind data. First, the ERA5 reanalysis wind field, which can be used for a long period at a high resolution, was regridded and synthesized using the asymmetric typhoon wind field calculated via the Generalized Asymmetric Holland Model of the numerical model named ADvanced CIRCulation model. The accuracy of the asymmetric typhoon synthesized wind field was evaluated using data obtained from Korea Meteorological Administration and Japan Meteorological Administration. As a result of the evaluation, it was found that the asymmetric typhoon synthetic wind field reproduce observations relatively well, compared with ERA5 reanalysis wind field and symmetric typhoon synthetic wind field calculated by the Holland model. The sea surface wind data produced in this study are expected to be useful for obtaining storm surge data and conducting frequency analysis of storm surges and sea surface winds in the future.

• Korean Journal of Microbiology
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• v.40 no.4
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• pp.348-354
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• 2004

The expression and antibacterial. activity of recombinant human lactoferrin (hLf) was studied from meth­ylotrophic yeast Pichia pastoris. The gene encoding hLf, isolated from human breast cDNA library, was subcloned into the expression vector, pPIC3.5K under the control of AOX1 promoter. The gene was integrated into the host chromosome and was identified by Southern blotting. The expression of the integrated gene was investigated by RT-PCR, Northern blotting, SDS-PAGE and Western blotting. Discrete band corresponding to hLf was detected from the SDS-PAGE, which was confirmed by Western blotting. The expression was also confirmed by RT-PCR and Northern blotting. The antibacterial activity of the recombinant hLf (rhLf) was investigated using Staphy­lococcus aureus ATCC 6538P and Micrococcus flavus ATCC 10240 as test organisms. The rhLf showed strong antibacterial activities against the bacteria. Furthermore, many Gram-negative animal pathogens such as E.coli ATCC8739, 25922, and Salmonella typhimurium 114 and 115, Pseudomonas fluorescens ID 963 I, P. aeruginosa KCCM 11802, and Gram-positive bacteria Bacillus mesentericus were also inhibited in their growth by the rhLf.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.21-33
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• 2008

In this study, the researcher examined archaeomagnetic secular variation of Chungcheong Region through measured data of archaeomagnet obtained from 34 relics, which discovered in the baked earth from varied historic sites within the region. Furthermore, the researcher closely reviewed regional differences of geomagnetic field in the domestic territory. Reviewing the comparison between the measured data of archaeomagnet in Chungcheong Region and the archaeomagnetic secular variation of Japan, which has difference in distance, it reveals a noticeable change in declination by tilting more than 10 degree toward East in the year of about A.D. 300, although the feature of whole variation is quite similar. In other period of times, it was confirmed that the regional differences of geomagnetic field in which the magnetic dip became deeper to some degree, and the declination was tilted westward a little bit. Such patterns do not differ significantly from the pattern of entire archaeomagnetic secular variation of our country, and even in the direct comparison to the data of Chungcheong Region, the distinct regional difference in both periods before and after Christian era was not confirmed. The fact may become clearer that, when the volume of the measured data of archaeomagnet increases further, and when more data connected with varied time period are filled, the problem such as deviation of the measurement period of archaeomagnet caused by the regional difference of geomagnetic field would not be worrisome issue, especially in Korean territory, judging from the measured data of archaeomagnet of historic relics in Chungcheong Region. Besides, as great efforts are being exerted in order to get the most reliable measured data as much as possible in taking both samples and measurement, it is thought that there would be no problem not only in the issue of deviation of the measurement period involving with the measured data of archeomagnet, but also in the aspect of reliability of data.