• Title/Summary/Keyword: 재자화

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A Study on the Mechanical Properties of Grout Materials Using a Magnetic Field Treated Water (자화수를 사용한 주입재의 역학적 특성에 관한 연구)

  • Chun, Byung-Sik;Yang, Hyung-Chil;Lee, Sang-Young
    • Journal of the Korean Geotechnical Society
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    • v.22 no.7
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    • pp.65-72
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    • 2006
  • This study is about the estimation of the mechanical properties of cement grout material using a magnetic field treated water instead of tap water. The water that passed through a magnetic system is called MFTW. Similar research indicates that 5% of cement dosage can be saved by decreasing bleeding of concrete and improving resistance to freezing. The reason why MFTW can improve characteristics of concrete can be explained by molecular structure of water. Magnetic force makes water clusters into single molecule or small ones. Hence, the activity of water is improved by the magnetic force. While hydration of cement particles is on progress, the MFTW can penetrate the core region of cement particles more easily. Therefore, the hydration can be carried out more efficiently and the compression strength of concrete is highly improved. The sample of the sodium silicate cement grout's homogel using the MFTW results in highly compressive strength increases in compressive strength approximately from 20% to 50%.

Purification and Characterizating of Recombinant Human Albumin from Hansenula polymorpha DL-1 (Hansenula polymorpha DL-1이 생산하는 재조합 알부민의 정제 및 특성)

  • 최근범;구선향;임채양;이동희;강현아;이상기
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.248-252
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    • 2001
  • Recombinant Human serum albumin (rHSA) was purified to near homogeneity from H, polymorpha using heat treatment, ultrafiltratipn and Phenyl Sepharose CL-4B and Mono Q column chro - matographies with a recovery yield of 60% The molecular weight of the purified rHSA was estimated to be about 65,000 Da by denaturing SDS-PAGE The N terminal amino acid sequence of the purified HSA determined by Edman degradation was turned out to be Asp- Ala- His- Lys- Ser- Glu- Ala, suggesting that the rHSA expressed in H, polymorpha was efficiently secreted and correctly processed at the cleavage site of secretion signal sequence. The purified human albumin showed the pI value identical to that of authentic human serum albumin.

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Synthesis of Polymer-Attached Triphenylamine Cation Radical (Polymer-Attached Triphenylamine 양이온 라디칼의 합성)

  • Cha, Ki-Hyuk;Jin, Myung-Jong
    • Applied Chemistry for Engineering
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    • v.5 no.3
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    • pp.438-442
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    • 1994
  • A very useful cation radical, triphenylaminium hexachloroantimonate, was quantitatively incorporated into insoluble polymer framework. The polymer cation radical was found to be effective as a catalyst in the Diels-Alder type dimerization and then shown to be reusable through several cycles of use.

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Magnetic Particles in Rainfalls: An Environmental Magnetic Evaluation (강수 함유 자성물질에 대한 환경자기학적 분석)

  • Baatar, Amarjargal;Yu, Yong-Jae
    • Journal of the Mineralogical Society of Korea
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    • v.23 no.2
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    • pp.99-106
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    • 2010
  • To evaluate a potential wash-out effect of rainfalls, a preliminary environmental magnetic test was attempted. Measurement of isothermal remanent magnetization (IRM) and intensive microscopic observations were carried out on the solid particles extracted from the rainfalls collected for the past year (2009) in Daejeon, Korea. Dust particles collected from the rain-free (daily dust) or dustheavy days (during the Asian dust storm event) were also used as a comparison. IRMs were unanimously low for the solid particles extracted from the rainfalls, indicating an efficient wash-out effect of rainfalls as long as the magnetic concentration is concerned. Electron microscopy identified carbonbearing material, (carbon-coated) magnetite, and quartz. It is highly likely that the carbon-containing particles were produced by anthropogenic fossil fuel combustion.

Expression of Invertase in Recombinant Saccharomyces cerebisiae Containing SUC2 Gene (SUC2 Gene을 갖는 재조합 Saccharomyces cerebisiae의 Invertase 발현특성)

  • 정상철;장재권;김인규;변유량
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.263-268
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    • 1989
  • To maximize the performance of recombinant cell fermentation process through optimizing environmental conditions, the production of invertase from recombinant Saccharomyces cerebisiae Containing SUC2 gene was studied as a model. The recombinant cells showed biphasic growth on glucose. Since the promoter of the SUC2 is regulated by the concentration of glucose in the medium, expression of invertase by recombinant yeast began when the glucose concentration decreased in a range of 0.25-0.4 g/L during the batch culture. Plasmid segregation occured frequently during glucose fermentation, and infrequently during ethanol oxidation. A rapid appearance of invertase activity with glucose was observed under nonaerated condition, and the maximum specific invertase activity was about 1.5 times as high as under aerobic condition, In fed batch culture, when n low level of glucose was continuously supplied to the tormentor after the time of glucose depletion during growth phase, specific and total invertase activity increased about 1.7 and 2.9 fold, respectively, in a batch culture.

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Redistribution of Welding Residual Stress and its Effects on Fatigue Crack Propagation (피로균열이 진전할 때 용접잔류응력의 재분포와 그 영향)

  • 이용복;조남익
    • Journal of Welding and Joining
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    • v.13 no.4
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    • pp.155-162
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    • 1995
  • Redistribution of residual stress and its effects during fatigue crack propagates from tensile residual stress region in weldment are investigated. Tests are performed by using welded CCT specimens of structual rolling steel (SS400) and it makes fatigue crack propagate from tensile residual stress region. For this study tension-tension loading type is selected by external loading condition and magnetizing stress indicator is used correctly to measure redistribution of residual stress according to fatigue crack growth and number of loading cycles. From this result, it is proved that redistribution of residual stress is mainly consist of residual stress released by fatigue crack growth. When fatigue crack propagates from tensile residual stress region residual stress are redistributed and it makes fatigue crack growth rate largely increase. Fatigue crack growth rate is low in case of redistributed residual stress compare with initial distributed residual stress.

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Re-Demagnetization Operation Characteristics Analysis of a Variable Flux Memory Motor Using Coupled Preisach Modeling and FEM (프라이자흐 모델과 유한요소법을 이용한 가변자속형 메모리 모터의 재자화, 감자 특성 해석)

  • Kim Gi-Bok;Kwon Sun-Bum;Lee Jung-Ho
    • Proceedings of the KIEE Conference
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    • summer
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    • pp.905-907
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    • 2004
  • This paper deals with the PM performance evaluations in a variable flux memory motor (VFMM) using a coupled finite element method (FEM) and Preisach modeling, which is presented to analyze the magnetic characteristics of permanent magnets. The focus of this paper is the operation characteristics evaluation relative to magnetizing direction and quantity of permanent magnets on re-, demagnetization condition in a variable flux memory motor(VFMM)

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Study on the Usability of Vitrified Mouse Hatched Blastocysts in Embryo Transfer (초자화 동결된 생쥐 완전탈출 배반포기배 이식에 관한 유용성 검토)

  • 이봉경;김은영;남화경;이금실;윤산현;박세필;임진호
    • Korean Journal of Animal Reproduction
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    • v.22 no.2
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    • pp.171-176
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    • 1998
  • This study was carried out to confirm whether the in vivo developmental potential of mouse hatched blastocysts (HBs) can be obtained by vitrification method using the cryoprotectant EFS35. The HBs ($\theta$ 130$\mu\textrm{m}$) were cultured in vitro until day 5 from zygotes produced in vivo and were equilibrated in 10% ethylene glycol(EG) for 5 min, and then were exposed or vitrified in EFS35 (35% EG, 18% Ficoll and 0.3M sucrose). After 30 min thawing, re-expanding HBs were transferred into one or both uterine horns of pseudopregnant recipients on day 3 (4~6 embryos /horn). Pregnancy rates of recipients and implantation were assessed by autopsy on day 15 of gestation. The results obtained in these experiments were summarized as follows : After thaw-ing, in vitro survival of HBs was not significantly different between exposed (65.5%) and vitrified(54.5%) group. Also, when the in vivo development potential was examined, total implantation was not different between control (58.5% ) and vitrified (41.0%) group, although the live fetus formation of vitrified group (24.0%) was significantly lower than that of control (58.3%) group (p< 0.05). These results suggested that vitrification freezing method of mouse HBs using EFS35 can be used to make wide the utility of embryo transfer of the more embryos produced in vitro.

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Evaluation of Image Usability by SEMAC Turbo Factor Change using Susceptibility Artifact Reduction (Susceptibility Artifact를 감소시키는 SEMAC 사용 시 Turbo Factor 변화에 따른 영상의 유용성 평가)

  • Choi, Young-Jae;Kim, Sang-Hyun
    • Journal of the Korean Society of Radiology
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    • v.13 no.1
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    • pp.31-37
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    • 2019
  • The study analyzes Non SEMAC and SEMAC to reduce susceptibility artifacts that may occur when performing magnetic resonance imaging(MRI) of metal patients. The Foot and Ankle Phantom was used as the experimental tool and the 3.8 cm general screw was used to make the magnetic susceptibility artifact. The experimental equipment was used 3.0T Magnetom Skyra and the area was measured with the 17th image where the signal off is the most noticeable in the obtained image. Statistical analysis was performed using the SPSS(Ver.25) program and the significance was assessed by the Wilcoxon Signed Rank Test. As a result, the area of Non SEMAC which is the lowest signal was $289.53{\pm}23.07197mm$. When the Turbo Factor was changed to 3, 4, and 5 after SEMAC use, it decreased to $125.02{\pm}7.45875mm$, $120.96{\pm}12.01704mm$ and $108.79{\pm}16.53498mm$, respectively. In conclusion, this study demonstrates that Using SEMAC with Turbo Factor effectively reduces the susceptibility artifacts.

Systems for Production of Calves from Hanwoo(Korean Cattle) IVM/IVF/IVC Blastocyst III. Vitrification and One-Step Dilution of Hanwoo Blastocyst (체외생산된 한우 배반포기배로부터 송아지 생스을 위한 체계 III. 한우 배반포기배의 초자화 동결과 1단계 융해)

  • Kim, E.Y.;Kim, D.I.;Park, N.H.;Weon, Y.S.;Nam, H.K.;Lee, K.S.;Park, S.Y.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Korean Journal of Animal Reproduction
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    • v.23 no.4
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    • pp.293-301
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    • 1999
  • This study was to examine whether Hanwoo IVM/IVF/IVC blastocyst can be successfully survived in vitro/in vivo after vitrification and one-step dilution. For vitrification, blastocysts were serially exposed in glycerol (G) and ethylene glycol(EG) mixtures[10% (v Iv) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then they were loaded in the straw, placed in cold nitrogen vapor for 3 min. and plunged into L$N_2$(-196$^{\circ}C$). One-step dilution within the straw was done in $25^{\circ}C$ and 36$^{\circ}C$ water for about 5 min. and 3 min., respectively. Recovered embryos after one-step dilution were cultured in cumulus cell mono-layered drop for 48 h or were transferred into recipient cows. When the embryo survival in vitro was assessed to re-expanded and hatched rates at 24 hand 48 h after one-step dilution, the results of vitrified group (85.4, 43.8%) was high, although these results were significantly lower than normal development (100.0, 63.3%) of control group, respectively (P<0.001, P<0.05). When in vitro survival of vitrified groups according to developmental stage was compared, the results of fast developed embryos (expanded blastocyst and early hatching blastocyst stage) were significantly higher than those of delayed developed one (early blastocyst stage) after one-step dilution (early hatching: 88.0, 48.0%: expanded: 81.1, 45.3%; early: 66.7, 14.3%) (P<0.05). Also, in case of in vitro survival of vitrified groups according to embryo age (day 7, 8 and 9), when embryo age was younger, in vitro survival was significantly higher (day 7: 67.3, 34.5%; day 8: 76.9, 40.7%; day 9: 60.9, 23.9%)(P<0.05). Finally, when in vivo development potential of vitrified and one-step diluted Hanwoo blastocysts was examined, 4 of 8 recipient (50%) cows became confirmed pregnant. These results demonstrated that our vitrification and one-step dilution technique can be applied easily and effectively on field trial without the equipment and embryological skills required for conservative dilution and transfer.

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