• Journal of Life Science
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• v.31 no.2
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• pp.199-208
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• 2021

Euonymus porphyreus, a species of plant in the Celastraceae family, is widely distributed in East Asia, especially in Southern China. The botanical characteristics of E. porphyreus have been reported, but its antioxidative and anticancer activities remain unclear. In this study, we evaluated the antioxidative and anticancer effects of ethanol extracts of E. porphyreus (EEEP) and the molecular mechanism of its anticancer activity in human lung adenocarcinoma A549 cells. The total polyphenol and flavonoid compound contents from EEEP were 115.42 mg/g and 23.07 mg/g, respectively. EEEP showed significant antioxidative effects with a concentration at 50% of the inhibition (IC50) value of 11.09 ㎍/ml, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. EEEP showed cytotoxic activity by increasing the SubG1 cell population of A549 cells in a dose-dependent manner. Apoptosis in A549 cells treated with EEEP was evident due to increased apoptotic cells and apoptotic bodies, as detected by Annexin V and 4,6-diamidino-2-phenylindole (DAPI) staining, respectively. EEEP-induced apoptosis resulted in increased expression of the First apoptosis signal (Fas), p53, and Bax, with decreased expression of Bcl-2 and subsequent activation of caspase-8, -9, and caspase-3, leading to cleavage of poly (ADP-ribose) polymerase (PARP). Collectively, these results suggest that EEEP may exert an anticancer effect by inducing apoptosis in A549 cells through both intrinsic and extrinsic pathways.

• Polymer(Korea)
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• v.39 no.3
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• pp.493-498
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• 2015

For biomaterials for skin regeneration with minimized inflammatory response, high bioactivity and biocompatibility are highly required. Also, it should have a porous microstructure to improve cell adhesion and growth. In this study, we extracted a new collagen source from duck's feet which is by-product, and made the shape of sponges from duck's feet collagen (DC) to compare with DBP and SIS. To analyze physical and chemical property of the scaffold, SEM and FTIR were used. MTT assay was used to measure the attachment and proliferation of NIH/3T3 in the scaffolds. RTPCR was used to evaluate the expression of proinflammatory cytokine. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to measure the ability of antioxidant activity. Overall, this study shows that DC scaffold is biocompatible and has good physical property. Additionally, DC scaffold shows the potential as wound healing biomaterials.

• Journal of Chest Surgery
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• v.30 no.8
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• pp.739-746
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• 1997

A variety of experiments concerning the development of ideal prosthetic grafts for correcting circumferential tracheal defects have been performed. The requirements for an ideal tracheal prosthesis are impermeability to air, consistency to prevent collapse, and acceptance by the host tissue causing a minimum inflammatory reaction, allowing fibroblastic infiltration and epithelialization. The synthetic material, polyurethane(PU), is known as a biocompatible polymer with an inert component. In this study, the tracheal prosthesis was made from microporous PU(30 micrometer in diameter) coated with gelatin and reinforced with isoplastic rings. This procedure provides the prosthesis with a compression strength. The out side diame er of the prosthesis was 20 mm with a length of 30 mm. The gelatin used in the study was obtained from pig skin and immobilized and cross-linked by irradiation(60 Co gamma ray) to promote host tissue incorporation and render the prosthesis epithelization after implantation. Animal experiments using 10 mongrel dogs were performed to compare three kinds of prosthesis; gelatin coated polyurethane graft, uncoated polyurethane graft, and prosthesisf pericadium complex graft. After 6 weeks of implantation, the epithelialization of implants was seen on the gelatin-coated and prosthesisfpericadium complex grafts. Implanted prosthesis were complicated by airway obstruction due to anastomosis granuloma. Early tracheal stenosis was found in the uncoated graft group. Two kind of anastomosis techniques were tested on the gelatin-coated prosthesis. Everted anastomosis resulted severe granuloma than the inverted anastomosis. In the prosthesislpericadium complex graft, bacteria and inflammation at a anastomotic site was found. Based on these results, gelatin coated porous polyurethane trachea prosthesis is biocompatible and may be useful in clinical application with further investigation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.2
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• pp.145-152
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• 2016

Epidermis is continuously regenerated by keratinocyte stem cells (KSCs) residing in basement membrane, which is critical to the survival of an organism. KSCs are believed to persist during the whole lifetime and generate an enormous number of keratinocytes, required for the maintenance of epidermis, through transit amplifying cells dividing definite times until they become differentiated. In this report, we have developed a phenolic compound, paeonol, purified from Moutan Cortex, as a KSC proliferation activator, by screening about 350 herbal compounds. The cell proliferation activation by paeonol is specific for KSC not for keratinocyte, and no significant difference in the expression of p63 protein, a KSC marker, in KSCs treated with paeonol was observed in FACS analysis with anti-p63 antibody. In the colony forming assay, paeonol-treated KSC showed improved colony forming activity more than 1.3 fold. In addition, the result of PCR array shows that the activity of paeonol is through several signal pathways involving stem cell functions. These results suggest that paeonol could enhance KSC proliferation activity without reduction in stemness and could be applied to cosmetics as a KSC activating ingredient.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.1
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• pp.22-28
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• 2019

Cement is a basic material for the construction industry and it requires high temperature sintering when manufacturing cement. $CO_2$ emissions from raw materials and fuels are recognized as new environmental problems and efforts are underway to reduce them. Techniques for reducing $CO_2$ in concrete are also recommended to use blended cement such as blast furnace slag or fly ash. In addition, the construction waste generated in the dismantling of concrete structures is recognized as another environmental problem. Thus, various methods are being implemented to increase the recycling rate. The purpose of this study is to utilize the inorganic raw materials generated during the dismantling of the structure as a raw material for the low carbon type cement binder. Such as, waste concrete powder, waste cement block, waste clay brick and waste textile as raw materials for low carbon type cement binder. From the research results, low carbon type cement binder was manufactured from the raw material composition of waste concrete powder, waste cement block, waste clay brick and waste textile.

• Journal of the Economic Geographical Society of Korea
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• v.19 no.4
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• pp.630-641
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• 2016

This paper attempts to analyse the processes, key actors and projects contributed to the urban renewal and creative city policies of the City of Maribyrnong, Victoria, Australia. The paper introduces how former military lands are redeveloped into new urban areas mixed with arts, cultural, commercial, residential and university activities. It articulates major actors involved in the creative city policies with governments, universities and non-government organisations. It is interesting to note that Maribyrnong uses Victoria University in vitalising declining old urban areas. It is also interesting to do the same that the city does adopt the concept of "creativity" in urban renewal policies; however, it does not use the same concent of creativity appeared in the textbooks of the major authors of creative city or creative economy, such as Richard Florida and John Howkins.

• Journal of Korea Foresty Energy
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• v.19 no.2
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• pp.79-85
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• 2000

Mortality patterns ad changes of vegetation in newly formed gaps were investigated to examine the succession process of Pinus densiflora forest located at Mt. Worak. The main findings from this study were as follows: ⑴ The forest was dominated by P. densiflora and Quercus variabilis in the oversotry, while Q. mongolica, Q. serrata and Fraxinus sieboldiana in the mid-story. ⑵ In all study areas, the standing dead type was the most common factor influencing gap-forming mortality. ⑶ DBH analysis showed that Pinus densiflora community was replaced by Quercus variabilis community first, and then by quercus mongolica and quercus serrata community. ⑷ Seedlings of P. densiflora were discovered only at dry sites of the gap.

• Resources Recycling
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• v.20 no.3
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• pp.3-11
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• 2011

Magnesium has been used as parts of vehicles, case materials of notebook PC and mobile phone, and its demand has been increasing recently. So until now, there has little magnesium scraps from the end of life vehicles or electronic parts, and most scraps has been generated from magnesium processing lines such as melting, die casting and machining. It is to review the present status of magnesium recycling. Here, domestic demand of magnesium, recycling amount and technologies used in domestic recycling companies were surveyed in recent years. In 2010, 8,840 tons of magnesium scraps were processed and used as raw materials for die casting products. The recycling ratio was estimated as 32.5%.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.2
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• pp.155-162
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• 2014

In this study, in order to know the application for cosmetic ingredient, the liposome contained camel milk was prepared and tested in human skin fibroblast. Collagen and hyaluronan synthase-3 (HAS-3) gene expression were increased by camel milk liposome in a concentration-dependent manner, whereas elastase activity and matrix metalloproteinase (MMP)-1 gene expression were inhibited. We also found that camel milk liposome regenerated UVB-damaged fibroblast. As the results, we suggest that the liposome contained camel milk is applicable for a potential cosmetic ingredient to improve anti-aging effect.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.136-137
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• 2015

이온성 고분자 금속 복합체(Ionic polymer metal composite, IPMC)는 전기 활성 고분자(Electro active polymer, EAP)중의 하나로 IPMC의 양 전극에 전기적인 자극을 가하면 굽힘 변형이 발생하고, 반대로 기계적인 자극이 주어지면 양 전극 사이에 전위차가 발생하여 전기를 얻을 수 있어 차세대 액추에이터와 센서로의 적용이 가능하다. 본 논문에서는 IPMC를 센서 소재로 사용하여 해양 환경 모니터링 센서에 전원을 공급하는 장치 개발을 설명하고자 한다.