• Title/Summary/Keyword: 재사용 간격

Search Result 277, Processing Time 0.034 seconds

돼지 난소의 황체세포의 체외배양시 TGF-${\beta}$1의 분비

  • 최재혁;김봉기;정학재;박진기;우제현;양병철;장유민;박수봉;성환후
    • Proceedings of the KSAR Conference
    • /
    • 2003.06a
    • /
    • pp.89-89
    • /
    • 2003
  • 본 연구는 난소의 황체를 체외배양시 TGF-$\beta$1의 황체내 발현을 조사하기 위해 수행되었다. 돼지 황체는 축산기술연구소에서 사육중인 돼지(체중 145$\pm$kg) 12두로부터 발정을 유도시켜 배란 후 약 48시간째 도축하여 난소를 회수하였다. 회수된 난소로부터 황체를 분리하여 세절한 후 0.25% collagenase용액(0.025mg DNase, 50mM EDTA, 50mM Dithio-threitol)으로 37$^{\circ}C$의 진탕 수조에서 30분간 배양하여 황체세포를 분리 회수하였다. 회수된 황체세포는 D-MEM용액(GIBCO, 10% FCS와 antibiotics 첨가)으로 2회 세척하여 1$\times$$10^{6}$live cell/$m\ell$이 되도록 희석하여 24 well culture plate(Corning, New Tork 14831)에 분주하여 $CO_2$ 배양기($CO_2$: 5%)에서 24시간 간격으로 2회 배양액을 교환해 48시간 동안 배양하였다. 배양된 황체 세포는 immunocytochemistry 방법으로 TGF-$\beta$1의 발현을 관찰함과 동시에 황체조직도 같은 방법을 사용하여 TGF-$\beta$1 의 발현 유ㆍ무을 관찰하였다. 그 결과 황체세포 그리고 황체 조직 뚜렷한 TGF$\beta$1의 발현을 확인할 수 있었다. 이 결과로서 TGF$\beta$1은 황체기능을 유지하는데 하나의 인자로서 작용하며 다른 인자들과의 상호작용을 시사하고 있다.

  • PDF

Interleaving Delay Diversity with Antenna Selection or the Multi-code WCDMA System (다중 코드 WCDMA 시스템에서 안테나 선택 기법을 적용한 인터리빙 딜레이 다이버시티 연구)

  • 정숙현;김낙명
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.29 no.6A
    • /
    • pp.600-606
    • /
    • 2004
  • An interleaving delay diversity scheme is investigated in this paperand the applications in the multi-code WCDMA system along with antenna selection diversity is proposed. The proposed algorithm utilizes interleaving and delayed duplicated transmission mechanism to mitigate the effect of noise and fading. The interleaved signal and the original signal are both transmitted at intervals to obtain time diversity without any increase in required capacity. By adjusting the number of retransmissions of the information signal properly, the system achieve the receive diversity efficiently. For transmission power efficiency the proposed system also applies antenna selection diversity. By computer simulation, it has been shown that the proposed algorithm achieves better performance than the conventional algorithms by more than 2dB.

Low-velocity Impact Damage of a Thick Graphite/Epoxy Case (Graphite/Epoxy로 만든 두꺼운 관의 저속 충격손상에 관한 연구)

  • 김형원;윤영주;나성엽
    • Journal of the Korean Society of Propulsion Engineers
    • /
    • v.4 no.2
    • /
    • pp.31-38
    • /
    • 2000
  • Pressure vessels by composite materials were damaged sometimes during manufacturing or assembling. The state and the size of damage by low-velocity tests were investigated in this paper. Impactors of various masses and various tup shapes were dropped freely in the range of 120mm height to 700mm height. Compared with hemispherical tup of 12.7mm diameter, for hemispherical tup of 25.4mm diameter the size of surface dent was smaller but the size of delamination was bigger.

  • PDF

Design of MediaFLO/GPS/Bluetooth Chassis Mode Antenna for Mobile Handset with Metallic Body (금속 몸체를 갖는 휴대단말기를 위한 MediaFLO/GPS/Bluethooth용 새시 모드 안테나의 설계)

  • Jung, Kang-Jae;Lee, Byung-Je
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.20 no.9
    • /
    • pp.829-834
    • /
    • 2009
  • In this paper, a HediaFLO/GPS/Bluetooth antenna is designed for mobile handset with metallic chassis. It is consisted of two metal plates with 7.6 mm gap, a connection plate and source between two plates. It can be analyzed as U-slot antenna of $0.5\;{\lambda}$, array of shorted patches each application bands. Simulated and measured performance of the proposed antenna show that it has enough possibility to reuse metallic chassis itself as radiator without any additional space for antenna.

Method of Repair Design by Analysis of Damage Mechanism of Elevated Aquaduct (수로교 손상 메커니즘 분석에 의한 보수설계 방법)

  • Lee, Soo-Gon;Byun, Hang-Yong;Song, Chang-Young
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.9 no.1
    • /
    • pp.243-250
    • /
    • 2005
  • In elevated irrigation aquaducts, one can observe a common damage pattern. That is, cracks, and crushing of concrete are usually repeated at a certain interval even if no faults are found in the design and construction of the structures. To investigate the causes of this damage, longitudinal deformations of several aquaducts have been measured. The analysis of the measured data suggests that the damages are mainly caused by cumulative repetition of extension and contraction due to temperature changes.

Ground Detection Method for Removement of Earth Field for Magnetic Guidance System (자계안내시스템용 지자계 제거를 위한 Ground 검출법)

  • Im, Dae-Yeong;Jung, Young-Yoon;Ryoo, Young-Jae
    • Journal of the Korean Institute of Intelligent Systems
    • /
    • v.16 no.5
    • /
    • pp.581-586
    • /
    • 2006
  • In this paper, describes ground detection method for removal earth field of magnet guidance system Magnetic guidance system is magnetic markers are installed just under the surface of roadway pavement and the magnetic fields generated these markers are detected by magnetic field sensor mounted of vehicles. vehicle is know lot lateral distance using magnetic field. But sensor is together measuring the magnetic field and earth field. It is operate error. Thus in this paper, proposed new method removing earth field or development experiment device via show the for practical and excellence.

The Treatment of Source Separated Food Waste by Mesophilic Anaerobic Digestion System with Leachate Recirculation (중온 침출수 재순환 혐기성 소화 시스템을 이용한 음식물류 폐기물 처리)

  • Cho, Chan-Hui;Lee, Byonghi;Lee, Yong-Woon
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.24 no.1
    • /
    • pp.31-40
    • /
    • 2016
  • In this study, mesophilic anaerobic digestion of source separated food waste was carried out by leachate recirculation system and methane gas was produced. Two systems - system A and B were fabricated and placed within water bath to maintain $36^{\circ}C$. Each system was comprised of an anaerobic bioreactor and a leachate tank. Leachate in bioreactor was separated through the screen located at 30 mm above the bottom and a pump was installed to transfer collected leachate to the leachate tank. Everyday, 2.5 L of the leachate was pumped from the bioreactor to the leachate tank for 30 min and transferred leachate was pumped back to the top of the bioreactor for 30min, sequentially. Source separated food waste used for this experiment was washed by water before transferring to the laboratory. Transferred food waste was warmed to $36^{\circ}C$ before being fed to bioreactors. System A was fed to 49.1 g VS (Volatile Solids) and System B was fed to 54.0 g VS at every two weeks, respectively. $NH_4{^+}-N$ and salinity were monitored to see the inhibition toward anaerobic bioreaction and it was found that concentrations of these materials were not high enough to affect the bioreaction. Although the food waste was fed biweekly for 112 days and 140 days at system A and B, respectively, there was no sludge withdrawal from each system. Average methane productions rates were 0.439 L $CH_4/g$ VS and 0.368 L $CH_4/g$ VS for system A and B, respectively.

Analysis of Microbial Community Change in Ganjang According to the Size of Meju (메주의 크기에 따른 간장의 미생물 군집 변화 양상 분석)

  • Ho Jin Jeong;Gwangsu Ha;Ranhee Lee;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
    • /
    • v.34 no.7
    • /
    • pp.453-464
    • /
    • 2024
  • The fermentation of ganjang is known to be greatly influenced by the microbial communities derived from its primary ingredients, meju and sea salt. This study investigated the effects of changes in meju size on the distribution and correlation of microbial communities in ganjang fermentation, to enhance its fermentation process. Ganjang was prepared using whole meju and meju divided into thirds, and samples were collected at 7-day intervals over a period of 28 days for microbial community analysis based on 16S rRNA gene sequencing. At the genus level, during fermentation, ganjang made with whole meju exhibited a dominance of Chromohalobacter (day 7), Pediococcus (day 14), Bacillus (day 21), and Pediococcus (day 28), whereas ganjang made with meju divided into thirds consistently showed a Pediococcus predominance over the 28 days. Beta-diversity analysis of microbial communities in ganjang with different meju sizes revealed significant separation of microbial communities at fermentation days 7 and 14 but not at days 21 and 28 across all experimental groups. The linear discriminant analysis effect size (LEfSe) was determined to identify biomarkers contributing to microbial community differences at days 7 and 14, showing that on day 7, potentially halophilic microbes such as Gammaproteobacteria, Firmicutes, Oceanospirillales, Halomonadaceae, Bacilli, and Chromohalobacter were prominent, whereas on day 14, lactic acid bacteria such as Pediococcus acidilactici, Lactobacillaceae, Pediococcus, Bacilli, Leuconostocaceae, and Weissella were predominant. Furthermore, correlation analysis of microbial communities at the genus and species levels revealed differences in correlation patterns between meju sizes, suggesting that meju size may influence microbial interactions within ganjang.

A study on the exhaust gas recirculation in a MILD combustion furnace by using a Venturi nozzle (MILD 이용한 배기가스 재순환에 관한 연구)

  • Ha, Ji Soo;Shim, Sung Hoon
    • Journal of Energy Engineering
    • /
    • v.22 no.4
    • /
    • pp.413-419
    • /
    • 2013
  • The present study used the MILD combustor, which has coaxial cylindrical tube. The outside tube of the MILD combustor corresponds to the exhaust gas passage and the inner side tube is the furnace passage. A numerical analysis was accomplished to elucidate the characteristics of exhaust gas entrainment toward the inner furnace with the changes of venturi nozzle geometrical parameters, nozzle position, nozzle gap between high pressure air nozzle and venturi nozzle, and with the change of high pressure nozzle inlet velocity. The entrainment flow rate for the case with the high pressure air nozzle attached at the exhaust gas wall has relatively small change with the change of nozzle gap. That for the case with the high pressure air nozzle exposed to the exhaust gas has monotonically increase with the change of nozzle gap. The flow rate ratio of entrainment flow rate has considerably increase tendency with relatively lower air inlet velocity, on the other hand, that with relatively higher air inlet velocity could be seen relatively small increase.

Evaluation of p-y Curves of Piles in Soft Deposits by 3-Dimensional Numerical Analysis (3차원 수치해석을 이용한 점성토 지반의 p-y 곡선 산정)

  • Lee, Si-Hoon;Kim, Sung-Ryul;Lee, Ju-Hyung;Chung, Moon-Kyung
    • Journal of the Korean Geotechnical Society
    • /
    • v.27 no.7
    • /
    • pp.47-57
    • /
    • 2011
  • The p-y curve has been used to design pile foundations subjected to lateral loading. Although the p-y curve has a large influence on the pile lateral behavior, p-y curves have not been clearly suggested. In this study, the p-y curve of clay was evaluated for drilled shafts in marine deposits by using 3-dimensional numerical analyses. First, the optimization study was performed to properly determine boundary extent, mesh size, and interface stiffness. The numerical modeling in the study was verified by comparing the calculated and the pile loading test results. Then, the p-y curves of single and group piles were evaluated from the parametric study. The selected parameters were pile diameter, pile Young's modulus and pile head fixed condition for a single pile, and pile spacing for group piles. Finally, the p-multiplier was evaluated by comparing the p-y curves of a single pile and group piles. As a result, the p-multiplier at pile spacing of 3D was 0.83, 0.67 and 0.78 for the front, middle, and back row piles, respectively, and showed values similar to those of O'Neill and Reese (1999). For the pile group with pile spacing larger than 60, the group effect can be ignorable.