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Increase of Cell Concentration by the Automatic Addition of Glucose and Ammonium to an Alcohol Distillery Wastewater Reutilized for Cultivating a Baker's Yeast : Automatic Analysis and Control of Ammonium Concentration with an On-line Flow Injection Analysis System (알콜증류폐액을 이용한 빵효모배양에서 Glucose와 Ammonium의 자동첨가에 의한 증균 : 온라인 FIA 시스템에 의한 Ammonium의 자동분석 및 제어)

  • 이형춘
    • KSBB Journal
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    • v.15 no.2
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    • pp.139-144
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    • 2000
  • Automatic addition of glucose and ammonium to an alcohol distille깨 wastewater and the control of them at low $\infty$ncentration were tried to efficiently increase the cell concentration of a baker's yeast c비tivated in that wastewater. Added g glucose was indirectly controlled to less than 116 mg/L by a method which used DO as control parameter. Ammonium was a automatically measured and controlled within the range of 7.0~27.7 mM by a homemade on-line system which adopted FIA a as measurement method. Maximum specific growth rate and biomass yield to glucose were $0.21 hr^{-1}$ and about 0.78 g/g, w which were significantly increased values in contrast to those of an experiment without ammonium control. Biomass yield to a ammonium was 11.3 gIg. Cell cone엉ntration could be increased from 2.6 g/L to 18.5 g/L by the add ion of glucose and a ammonium.

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Bacillus cereus ASK-202에서 cloning 된 agarase의 물리 ${\cdot}$ 화학적 특성

  • Hwang, Seon-Hui;Ha, Sun-Deuk;Kim, Bong-Jo;Gong, Jae-Yeol
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.534-537
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    • 2002
  • An agarase gene from Bacillus cereus ASK202 was expressed in high levels by E. coli BL21(DE3)/pEBA1 using pET28a(+) vector system with the inducible T7 promoter in the presence of isopropyl- ${\beta}$ -thiogalactopyranoside. The open reading frame encodes 761 amino acid residues with a calculated molecular weight of 83,300 daltons and a potential signal peptide about 36 amino acid residues at the N-terminus. E. coli BL21(DE3)/pEBA1 produce 1280 unit/ ${\ell}$ of agarase. The optimum physical condition for the agarase activity was pH 5.6, and $40^{\circ}C$, respectively. The agarase activity was stable up pH $4.0{\sim}9.0$ and $4{\sim}40^{\circ}C$. The km and maximum rate of metabolism for agar were 0.068mg/$m{\ell}$ and 0.094mg/$m{\ell}{\cdot}min$, respectively.

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Axial Solid Holdup in a Circulating Fluidized Bed Plasma Reactor under Reduced Pressure (감압 순환유동층 플라즈마 반응기의 축방향 고체체류량)

  • Park, Sounghee
    • Korean Chemical Engineering Research
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    • v.54 no.4
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    • pp.527-532
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    • 2016
  • The effects of gas velocity and solid circulation rate on the axial solid holdup distribution have been determined in a 10 mm-I.D. ${\times}$ 800 mm-high circulating fluidized bed plasma reactor under reduced pressure (1torr). Polystyrene polymer powder and nitrogen gas are used as solid and gas materials respectively. The change of solid circulation rate by a large gas flow rate of the riser (40~80 sccm) is also possible by a relatively small gas flow rate of the solid recirculation part (6.6~9.9 sccm). The solid circulation rate in the reactor under reduced pressure increases with increasing aeration velocity in the solid recirculation part. The axial solid holdup in the riser decreases from the dense at the bottom to the dilute phase at the top section of the riser. Solid holdups at the axial positions in the riser increase linearly with increasing solid circulating velocity. From these results, we could determine the position of plasma load for good plasma ignition, maintain and plasma reaction.

A Design of Permission Management System Based on Group Key in Hadoop Distributed File System (하둡 분산 파일 시스템에서 그룹키 기반 Permission Management 시스템 설계)

  • Kim, Hyungjoo;Kang, Jungho;You, Hanna;Jun, Moonseog
    • KIPS Transactions on Computer and Communication Systems
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    • v.4 no.4
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    • pp.141-146
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    • 2015
  • Data have been increased enormously due to the development of IT technology such as recent smart equipments, social network services and streaming services. To meet these environments the technologies that can treat mass data have received attention, and the typical one is Hadoop. Hadoop is on the basis of open source, and it has been designed to be used at general purpose computers on the basis of Linux. To initial Hadoop nearly no security was introduced, but as the number of users increased data that need security increased and there appeared new version that introduced Kerberos and Token system in 2009. But in this method there was a problem that only one secret key can be used and access permission to blocks cannot be authenticated to each user, and there were weak points that replay attack and spoofing attack were possible. Hence, to supplement these weak points and to maintain efficiency a protocol on the basis of group key, in which users are authenticated in logical group and then this is reflected to token, is proposed in this paper. The result shows that it has solved the weak points and there is no big overhead in terms of efficiency.

Effect of Prefrontal lobe Neurofeedback Training for reducing Adolescent Theta wave (청소년기 세타파 감소를 위한 전전두엽 뉴로피드백 훈련 효과)

  • Byun, Youn-Eon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.12
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    • pp.459-465
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    • 2017
  • This research aims to assess whether neurofeedback training can reduce theta waves in adolescents. The experiment was conducted on 35 early youths living in Gyeonggi-do at youth counseling centers during April-October. According to circumstances and opinions of participants in the pre-brain analysis, they were classified into a non-training group (A), 12-week training group (B), and 24-week training group (C), containing 10, 15, and 10 members, respectively. EEG measurement and neurofeedback training was performed using the prefrontal 2-channel NeuroharmonyS and Brain Optimization program. EEG data was processed utilizing Brain Analysis ver1.3. Deducted data was converted to SPSS 21.0 to enable statistical processing. As a strategy to reduce theta through the Beta increase training, we applied the appropriate Alpha, SMR, Beta low reward training to the individual. Study results confirmed that theta waves of adolescents decreased through the prefrontal neurofeedback training. Groups (B) and (C) exhibited a greater decrease in theta waves compared with the control group.

Policy Direction Setting through Comparative Analysis of Foreign Smart City Policies (국외 스마트시티 추진 정책 비교 분석을 통한 성과 확산 방향 설정)

  • Jung, Seunghyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.9
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    • pp.151-160
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    • 2020
  • Various smart city projects have been actively promoted as important policies of governments in various countries. The key to the success of smart cities led by governments is to develop sustainable governance through the expansion and proliferation of outcomes. This study comparatively analyzed the smart city policies of the USA and Europe from viewpoints of outcome expansion and proposed policy directions for smart cities in Korea. The comparison items were case cities, the expansion of smart city technologies and services, the measurement of outcomes, the accumulation of knowledge and information, and standardization. This study found that the items of each index were linked for the purpose of assessment, expansion, and the creation of successful cases in the USA and Europe. Based on our analysis, four policy directions were proposed that included the early provision of follow-up measures for case cities, the development of a project performance assessment system, the provision of an integrated knowledge accumulation system, and an earnest promotion of industry activation policies.

An XML-based Content Management System supporting Dynamic Content Caching (동적 컨텐츠 캐싱을 지원하는 XML 기반의 컨텐츠 관리 시스템의 구현)

  • Koo Heung-Seo
    • Journal of the Korean Institute of Intelligent Systems
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    • v.15 no.7
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    • pp.794-799
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    • 2005
  • In this paper, We describe the XML-based Web content management system that supports the efficient dynamic content publishing environment. EasyCM is designed based on Cocoon2 that is the XML publishing framework. We propose the publishing mechanism to support the efficient dynamic content publishing environment to expand into the available dynamic content caching to Cocoon2. Publishing mechanism of EasyCM draws XML object from content repository, associates XML with XSLT, creates and caches content components preprocessing HTML transformation process, and publish web pages constructed into cached content component. For supporting more efficient caching, EasyCM supports also content component update, two update method that is immediately-update and delay-update for updated content component.

Adaptive Output-feedback Neural Control of uncertain pure-feedback nonlinear systems (불확실한 pure-feedback 비선형 계통에 대한 출력 궤환 적응 신경망 제어기)

  • Park, Jang-Hyun;Kim, Seong-Hwan;Jang, Young-Hak;Ryoo, Young-Jae
    • Journal of the Korean Institute of Intelligent Systems
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    • v.23 no.6
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    • pp.494-499
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    • 2013
  • Based on the state-feedback adaptive neuro-control algorithm for a SISO nonaffine pure-feedback nonlinear system proposed in [15], an output-feedback controller is proposed in this paper. The output-feedback adaptive neural-net controller for the considered nonlinear system has not been previously proposed in any other literatures yet. The proposed output-feedback controller inherits all the advantages of [15] such that it does not adopt backstepping and this results in relatively simple control and adapting laws. Only one neural network is required for the proposed adaptive controller. The proposed neural-net control scheme expands the applicable class of nonlinear systems.

CGE 모형을 이용한 다목적댐 운영의 경제파급효과분석: 용수공급기능을 중심으로

  • Jeong, Gi-Ho;Kim, Jae-Hyeon
    • Environmental and Resource Economics Review
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    • v.21 no.1
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    • pp.129-156
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    • 2012
  • This study analyzes the contribution to the national economy of the stable water supply through managing multi-purpose dam. For the analysis, we consider 17 major multi-purpose dams and build a CGE model with summer water and winter water being the production factors as the base year of 2007. We analyze the economic impact of meeting water demand due to the dam management and estimate the risk premium of reducing the uncertainty of water supply. The analysis results show a significant production decrease in the industries of agriculture, forestry and fisheries and tap water as well as the food and beverage industry using the former industries' output as intermediates in the production and show an production increase largely in steel industry and electronic and electrical industries. Being compared to the benchmark solution, GNP is analyzed as being reduced by 0.22~0.68%. Meanwhile, the risk premium is estimated to be about 4 billion to 24 billion won for the value 01 the measure of relative risk aversion in the range 01 0.5 to 3.0.

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Analysis and the Conservation Treatment of the Paper Relics of the Seonwon Temple Area (선원사지(禪源寺地)출토 지류 유물의 분석 및 보존 처리 방법)

  • Lim, Ju-Hee;Park, Chi-sun
    • Journal of Conservation Science
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    • v.12 no.1 s.15
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    • pp.71-82
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    • 2003
  • The paper relics excavated from the 4th excavation of the historical spot No.259 Seonwon temple area were analyzed and conserved. Because these works remained folded, the most important conservation treatment was a separation. This process depended on the state of the works: dry state works were separated using Gore-Tex chamber and the filtrated water, wet state works were separated through vacuum freeze drying method. Analyses of the paper relics performed were examination on the paper to find its distinction, binocular microscope and SEM on fiber, and XRF on character ingredient. This study presumed the times though $C^{14}$ dating of these works. The works were able to be classified through analysis and conservation treatment. This separation made lost part of the works conserve an archetype. This study presented the application of conservation methods on the paper relics which are in the bad condition and obtained information through carbon dating of it.

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