• Korean Journal of Animal Reproduction
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• v.25 no.1
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• pp.51-61
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• 2001

This study was conducted to determine developmental ability of reconstructed embryos by nuclear transfer using somatic cell of Korean bull with high genetic value. Fibroblast cells obtained from ear biopsy of the bull were cultured in Dulbecco's Modified Eagle's medium (DMEM) at 37$^{\circ}C$ in air containing 5% $CO_2$. The cummulus-oocyte complexes were collected from slaughterhouse and were matured in vitro for 20 h in TCM 199 culture medium and the oocytes were then enucleated in modified phosphate buffered saline with cytochalasin B. Matured bovine oocytes were enucleated by aspirating the first polar body and metaphase chromatin using a beveled pipette in modified phosphate buffered saline. The ear fibroblast cells were fused into enucleated oocyte by electrical stimulation. The reconstructed oocytes were activated with ionomycine and 6-dimethylaminopurine, and then cultured in CR1aa medium for 7.5 days. Out of 524 bovine eggs reconstructed by nuclear transfer 65.6%(277/422) embryos were cleaved, and 30.7% (85/277) cleaved embryos were developed to the morula to blastocysts. There was no difference of developmental ability in vitro of reconstructed embryos regardless of donor cell passages. In order to determine fate of foreign mitochondria of donor nucleus, the Mito Tracker stained cells were fused into enucleated oocytes. The donor mitochondria were detected early stage of embryos, but disappeared rapidly. The developmental ability of reconstructed embryos was not impaired by Mito Tracker treatments. The results indicate that viable reconstructed embryos can be producted by nuclear transfer using somatic cell of Korean bulls.bulls.

• Korean Journal of Environmental Agriculture
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• v.30 no.1
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• pp.60-67
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• 2011

BACKGROUND: Because dried herbal medicines have many active ingredients, it is not easy to determine the residue amount after extraction, partition and clean up of pesticides from them. Especially, liquid-liquid partition method is consuming many times and solvents. Macroporous diatomaceous earth(MDE) column was used to replace the separatory funnel for liquid-liquid extraction with dichloromethane to analyze the endosulfan and its metabolite. METHODS AND RESULTS: The residue analysis method using MDE column instead of liquid-liquid partition for determining insecticide endosulfan and its metabolite in 4 dried herbal medicines was developed by GC/MS. As a result, the recovery rates of the pesticides in 4 herbal medicines were ranged from 80.3 to 93.5% for ${\alpha}$-endosulfan, from 81.0 to 100.3% for ${\beta}$-endosulfan and from 80.6 to 95.6% for endosulfan sulfate, respectively. The coefficients of variation for triplicate were ranged from 1.1 to 3.4%. CONCLUSION: The improved methods are more ecofriendly, safer, faster and less laborious than conventional method by KFDA.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.7
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• pp.1447-1454
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• 2017

The realtime test data topic means that process for the data efficiently from many kinds of measurement device at the test range. There are many measurement devices in test range. The test range require accurate observation and determine on test object. In this realtime test data slaving framework system, the system can produce variety of test informations and all these data also must be transmitted to test information management or display system in realtime. Using RTI DDS(Data Distribution Service) middle ware Ver 5.2, we can product the efficiency of system usability and QoS(Quality of Service) requirements. So the application user enables to concentrate on applications, not middle ware. As the reason, Complex function is provided by the DDS, not the application such as Visualization Software. In this paper, I suggest the realtime test data topic on slaving framework of realtime test data based on DDS at the test range system.

• The KIPS Transactions:PartD
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• v.10D no.5
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• pp.763-772
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• 2003

Design patterns are known widely by the techniques to improve software qualify. The design patterns are efficient solutions for problems occurring frequently in software development. Recently there are wide researches for design patterns to find them and to verify usability for them. But there are very few researches to define systematic development approaches about constructing application using design patterns. In this paper, we propose an approach for the pattern-oriented software development process using incremental composition for design patterns. For this proposal, first we define a development process using design patterns, propose technique for incremental composition for design patterns and view ports for software in the process. Also we deal with the problem of efficient traceability and maintenance to design patterns in the software system. And we use a feedback framework system as an illustrative example to show how the process can be used to develop the pattern-oriented software. In the development of software, the systematic development approach and usage increase efficiency to develop the system by using design experience and technique early in the development lifecycle. Therefore the system will assure the high stability and reusability and offer the low cost and time for development.

• KIPS Transactions on Computer and Communication Systems
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• v.6 no.9
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• pp.377-384
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• 2017

The performance improvement of a single core processor has reached its limit since the circuit density cannot be increased any longer due to overheating. Therefore, the multicore and manycore architectures have emerged as viable approaches and parallel programming becomes more important. Haskell, a purely functional language, is getting popular in this situation since it naturally supports parallel programming owing to its beneficial features including the implicit parallelism in evaluating expressions and the monadic tools supporting parallel constructs. However, the performance of Haskell parallel programs is strongly influenced by the performance of the run-time system including the garbage collector. Though a memory profiling tool namely GC-tune has been suggested, we need a more systematic way to use this tool. Since GC-tune finds the optimal memory size by executing the target program with all the different possible GC options, the GC-tuning time takes too long. This paper suggests a basic divide-and-conquer method to reduce the number of GC-tune executions by reducing the search area by one-quarter for every searching step. Applying this method to two parallel programs, a maximally independent set and a K-means programs, the memory tuning time is reduced by 7.78 times with accuracy 98% on average.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.37-51
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• 2018

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

• Membrane Journal
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• v.26 no.1
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• pp.70-75
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• 2016

The reuse of wastewater is being diffused to manage and develop the water resources. Generally, the treated wastewater is discharged to the river after being treated to meet the effluent quality standard or reused for diverse uses through the reprocessing. And recently, as the reuse of the treated wastewater is activated, the technologies to utilize for the high quality water resources such as industrial water by reusing the wastewater with Membrane Distillation (MD) are under development. In this study, the direct contact membrane distillation (DCMD) process has been applied to treat sewage discharge water for water reuse. The laboratory scale experiment was performed by using a hydrophobic PVDF membrane with the pore size of $0.22{\mu}m$. The influence of operating parameters, such as feed temperature, feed flow rate, feed concentration, on the permeate flux and rejection has been investigated. All filtration tests were conducted till the feed volume reached a concentration factor of 3.0. Thus, the operating period ranged between 19 hr and 49 hr depending on filtration performance. The results showed that above 92% of TN, TP, COD and TOC in the feed could be rejected regardless of an operating condition. The water flux was ranged from 13.8 to 20.3 LMH. The lowest flux was obtained at the operating condition with the feed temperature of $50^{\circ}C$ and feed flow velocity of 500 mL/min while the highest one was measured with $60^{\circ}C$ and 900 mL/min. When the concentration factor reached 3.0, water flux declined by approximately ranged from 14.5% to 33.3%. But the fouling in MD is almost fully reversible, with more than 90% recovery of permeate water flux following a DI water rinse without the addition of chemical cleaning reagents.

• The Journal of Society for e-Business Studies
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• v.11 no.1
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• pp.1-23
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• 2006

As data on the Web is more and more in XML due to proliferation of Web applications such as e-Commerce, it is strongly required to rapidly process XML queries. One of such techniques is XML query caching. For frequently submitted queries, their results could be cached in order to guarantee fast response for the same queries. In this paper, we propose techniques for XML query performance improvement whereby the set of node identifiers(NIS) for an XML query is cached. NIS is most commonly employed as a format of XML query result,, consisting of the identifiers of the XML elements that comprise the query result. With NIS, it is suitable to meet the Web applications data retrieval requirements because reconstruction and/or modification of query results and integration of multiple query results can be efficiently done. Incremental refresh of NIS against its source updates can also be efficiently done. When the query result is requested in XML, however, materialization of NIS is needed by retrieving the source XML elements through their identifiers. In this paper, we consider three different types of NISs. proposing the algorithms of their creation, materialization, and incremental refresh. All of them were implemented using an RDBMS. Through a detailed set of performance experiments, we showed the efficiency of the proposed XML query caching techniques.

• Journal of Korea Soil Environment Society
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• v.2 no.1
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• pp.83-90
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• 1997

Aiming at the treatment of large volumes of gas with a low concentration of poorly water soluble VOC(Volatile Organic Compound), a new system is proposed: the combination absorption tower/bioreactor. In the scrubber part of the bioscrubbing system, the contaminating compounds are absorbed in a aqueous phase. The contaminated scrubbing liquid is transported to the bioreactor, where the compounds are biodegraded by aerobic microorganisms (mainly to carbon dioxide, water, and biomass). In this study, separation of a volatile organic compound(VOC) out of a waste gas stream has been carried out using a re-cyclable high boiling point extrant(HBE). The liquid stream containing a high boiling point entrant(HBE) scrubs the gas stream in a direct gas-liquid countercurrent contacting operation in a packed tower for the removal of said component from the gaseous stream. A packed-bed column using Pall Ring was set up in order to simulate practical conditions for the scrubbing tower. The liquid stream transported to the bioreactor is recovered and recycled to the scrubber. The model gas, which contained 400 mg/$\textrm{m}^3$ of toluene, at a rate of 100 L/min, flowed into the packed column where the scrubbing liquid trickled over the packing in countercurrent to the rising gas at 10~15L/min. The bioscrubber designed for large volume air streams containing VOCs showed removal efficiency up to 80% in an optimum operating conditions during the tests fer removing toluene from an air stream by scrubbing the air stream with HBE.

• Tunnel and Underground Space
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• v.17 no.5
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• pp.411-427
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• 2007

Reliability in tunnel analysis is necessary to accomplish technically sound design and economical construction. For this, a thorough understanding of the construction procedure including the ground-support interaction has to be obtained. This paper describes a proper modelling technique to simulate the behavior of the steel fiber reinforced shotcrete (SFRS) which maintain the supporting capability in post-failure regime. The additional supporting effect of the steel support was also verified by 3-D analyses and a new load distribution factor were proposed. The use of the plastic moment limit (PML) alone can eliminate the occurrence of the awkwardly high tensile stress in the shotcrete and can successfully model the post-peak ductile behavior of the SFRS. But with this method, moment is limited whenever the stress caused by moment reaches tensile strength of the shotcrete irrespective of the stress by axial force. Therefore, it was necessary to find a more comprehensive method which can reflect the influence of the moment and axial force. This can be accomplished by the proper use of "liner element" which is the built-in model in FLAC. In this model, the peak and residual strength as well as the uniaxial compressive strength of the SFRS can be specified. Analyses were conducted with these two models on the 2-lane road tunnels excavated in class IV and V rock mass and results were compared with the conventional elastic beam model. Results showed that both models can reflect the fracture toughness of the SFRS which could not be accomplished by the elastic beam model.