• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.93-93
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• 1999

4품종의 알팔파 (Medicago sativa L.) 종자로부터 직접 캘러스를 유도하고, 형성된 캘러스로부터 식물체를 재분화하는 조건을 확립하였다. 공시품종중 "Vernal" 품종이 캘러스 유도 및 재분화에서 가장 우수한 것으로 판명되었으며, SH(Schenk and Hildebrandt), MS (Murashige and Skoog), N6 (Chu) 배지중에서 SH 배지가 캘러스 유도와 재분화시에 모두 유리한 것으로 나타났다. 캘러스 유도 및 증식시에는 2,4-D를 3mg/$\ell$ 농도로 첨가한 SH-3 배지에서 가장 효율이 좋았으며, kinetin을 첨가하거나 kinetin의 농도가 높을수록 캘러스 형성 정도는 급격히 저하되었다.(중략)저하되었다.(중략)

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.77-82
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• 2000

This study was conducted to determine the inheritance of the ability of callus formation and plant regeneration in seed cultures of rice. The culturabilities of three Japonica rices,'Chucheongbyeo', 'Nagdongbyeo', and 'Daeribbyeo 1', were higher than those of Tongil type cultivars, 'Milyang 23' and 'Samgangbyeo 'The frequency for callus growth in F₂ populations of the three crosses, 'Milyang 23/Chucheongbyeo', 'Milyang 23/Daeribbyeo 1', and' Samgangbyeo/Nagdongbyeo', revealed a nearly normal distribution. The broad-sense heritability estimated from the ability of callus formation in the crosses were ranged from 83.8% to 90.1%. The frequency distribution of plant regenerability in F₂ population of 'Milyang 23/Daeribbyeo 1' showed a continuous variation. But the segregation mode of plant regenerability from seed-derived callus in the F₂ progenies of 'Milyang 23/Chucheongbyeo' and 'Samgangbyeo/Nagdongbyeo' appeared to fit the expected 3 : 1 ratio for the high and low regenerability. These results suggest that the high plant regenerability of 'Chucheongbyeo' and 'Nagdongbyeo' was regulated by a single dominant gene.

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.822-824
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• 2007

The efficient system for plant regeneration from cotyledon and primary feat explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety 'Jungtongpogi' in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety' Chungchima', which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from prima leaf explants in KN medium. Kl medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety 'Jungtongpogi' were cultured on KN medium. In case of varie쇼 ‘Chungchima', efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.143-148
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• 1995

The competence of callus formation and plant regeneration from root derived callus was higher in japonica cultivars than those of Tongil-type cultivars of rice. A japonica type cultivars Yeongdeogbyeo, showed the highest capacity (13%) for plant regeneration from root calli of 6 cultivars tested. The callus induced from seed and root tissues maintained higher capacity for plant regeneration during 7 passages of subculture on N$_{6}$ solid media at 2-week intervals. The maximum frequency (2 x 10$^{5}$ mL) of round cells and their cell colonies showed about 24 days after suspension culture of root-derived callus in N$_{6}$ medium with lmg/L 2,4-D, 300mg/L casein hydrolysate, 10mM L-proline, 20g/L sucrose and 30g/L sorbitol. The frequency of somatic embryo formation in suspension cultures of root-derived callus increased with prolonged advance of subculture time from 30 to 90 days, but their regenerative capacities decreased.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.267-272
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• 1998

We confirmed conditions for callus formation and plant regeneration of five varieties of orchardgrass (Dactylis glomerma L.). Among five varieties of orchardgrass, "Hapsung 19" expressed the highest rate for both of callus formation and plant regeneration. Otherwise, among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu), MS and N6 medium were highest degree of efficiencies in callus formation and plant regeneration, respectively. In this study, we determined volume of hormones appended in media; $3\;mg/\;{\ell}$ ofdicamba for callus formation and $1\;mg/\;{\ell}$ of NAA (I-naphtalene acetic acid) and $5\;mg/\;{\ell}$ kinetin (6-furfurylaminopurine) for plant regeneration were appended in their media. We obtained orchardgrass plants from callus about 50~80 days after transferred to regeneration media.ation media.

• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.496-507
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• 2023

The in vitro organogenesis is one of important issues in plant embryology, and somaclonal variations are existing in calli and/or regenerants induced from a process of the organogenesis with in vitro circumstances. In this study, expressions of organogenesis-related genes were evaluated and genetic stability of regenerants derived from the process of in vitro organogenesis were measured using ISSR markers in Imperata cylindrica 'Rubra', Poaceae. The expressions of organogenesis-related genes were detected all of regenerants at the process of the organogenesis. All ISSR markers produced with an average of 71 bands per in vitro-cultured regenerants, and the scorable bands were varied from two to eight with an average of 5.14 bands per a primer. The polymorphism rates of the in vitro regenerants were higher than that of mother plants (1.4%), showing 4.1% (pot-cultured regenerants), 4.3% (field-cultured regenerants), 4.2% (in vitro-cultured regenerants), 5.6% (calli with green shoots) and 1.4% (calli), respectively. The genetic similarity matrix (GSM) among all accessions ranged from 0.747 to 1.0 with a mean of 0.868. GSM of the regenerants showed differences (from 0.972 to 1.00) compared with that of mother plants (0.991). According to the clustering analysis, two independent groups were divided into; the one is mother plants and regenerants cultured at room and open field, the other is regenerants cultured in vitro. The results give a new insight for understanding the dynamics of organogenesis in monocot plant.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.203-209
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• 2013

Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS) vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01, 0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L) for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.765-767
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• 1999

Experiments were conducted to find out the optimum cultural conditions for adventitious shoot regeneration from leaf segments of Gypsophila paniculata L. Thidiazuron (TDZ) was remarkably effective for the regeneration of leaf segment in Gypsophila paniculata compared with BA and kinetin. TDZ showed the highest rate of regeneration at $3.0mg{\cdot}L^{-1}$, while kinetin did not affect the regeneration. BA in the medium increased vitrification. Shoot formation efficiency was much higher on $0.3mg{\cdot}L^{-1}$ of IAA-containing media than NAA-containing media. Regeneration of leaf segments was induced with the agar concentrations of 1.0, 1.2 and 1.6%. Dark treatment at the initial stage of the culture increased the rate of regeneration up to 75%. The leaf explants from the 3rd subcultured stock plants after meristem culture, showed the highest adventitious shoot regeneration efficiency.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.89-89
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• 2018

우리나라, 중국 및 일본을 포함한 동북아시아가 원산이며 바이오매스량이 많은 억새(Miscanthus spp.)는 바이오에너지 생산을 위한 원료작물로서 가치가 높아, 바이오에탄올 생산용 원료작물로 주목을 받고 있다. 독일 등 유럽과 미국에서는 바이오에탄올 생산용 작물로 주로 종간 교잡 이질 3배체인 불임성 억새(M. x giganteous)를 대상으로 연구하고 있다. 이렇게 단일유전형을 갖는 품종의 재배에는 특정 병과 해충에 약하며 자연재해에도 취약성을 나타내므로 억새가 바이오에너지작물로 자리 잡기 위해서는 다양한 유전형의 억새 품종 개발이 필요하다. 본 연구는 우리나라의 자생 억새 3종을 기내배양하고 탈분화 및 재분화 시스템을 구축하여 억새 품종 육성 시 효율을 높이기 위해서 실시하였다. 억새 종자로부터 캘러스의 유도는 MS배지보다 N6배지 에서 좋았으며, 식물생장조절제로 2,4-Dichlorophenoxyacetic acid (2,4-D)와 6-Benzyl aminopurine (BA)를 조합처리한 처리구보다 2,4-D만을 단독처리하였을 때 캘러스 유도율이 더 높았다. 억새 종에 따른 캘러스 유도율은 물억새가 가장 낮고, 거대억새가 가장 높았으며, 3 ~ 5 mg/L의 2,4-D가 첨가된 N6배지에서 배발생 캘러스(embryogenic callus)가 발생하였다. 억새 신초 및 줄기의 절간에서의 캘러스 유도율은 전반적으로 종자에 비하여 낮았으며, 미성숙화기로부터의 캘러스 유도는 억새 종에 따른 차이가 없었으며, 5mg/L의 2,4-D가 첨가된 배지에서 캘러스 유도율이 가장 높게(90 ~ 95%) 나타났다. 형성된 배발생 캘러스로부터 식물체의 재분화는 N6배지에서는 재분화 식물체가 발생하지 않았고, 1 ~ 3mg/L의 BA와 0.1ml/L의 1-Naphthaleneacetic acid (NAA)가 첨가된 MS배지에서만 식물체가 재분화되였다.

• Korean Journal of Plant Tissue Culture
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• v.21 no.2
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• pp.65-68
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• 1994

Plant regeneration from the stem tissue of Orostachys japonicus A. Beiger was investigated. The calli derived from shoot apex when apex when cultured on Murashige and Skoog (MS) medium supplemented with 4mg/L 2,4-dichlorophenoxyacetic acid (2,4-D)and 2 mg/L benzyl aminopurine (BAP). The calli were developed into shoot to MS medium with 0.5mg/L NAA and 2mg/L and into root with 1mg/L kinetin. The reddish pigment which might be essential for the rootregeneration was observed in the tip of regenerated root.