• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.584-590
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• 2011

Wound inducible P450-Esg cDNA, one of cytochrome P450 gene family, was isolated from shoot of Euiseong garlic cultivar. P450-Esg cDNA possesses highly conserved heme-binding domain in the nucleotide sequence, and 1,419 bp of open reading frame (ORF) coding of 473 amino acids. Based on the nucleotide sequence analysis of P450-Esg homologous from twelve garlic cultivars, two domains, one domain between 472 to 510 bp, and the other between 1,210 to 1,249 bp from start codon (ATG), showed various nucleotide polymorphism among cultivars. Sequence of heme-binding domain in P450-Esg homologous, which is located at the domain between 1,210 to 1,240 bp from start codon, showed various nucleotide polymorphism as well as amino acid sequence polymorphism among twelve garlic cultivars. Anther domain, between 472 to 510 bp from start codon, showed exactly same amino acid sequence in the twelve garlic cultivars, but there were various single nucleotide polymorphism to the cultivars.

• Korean Journal of Plant Resources
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• v.23 no.5
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• pp.458-464
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• 2010

Twelve garlic cultivars collected from Korea and Mongolia were evaluated genetic similarity and diversity by RAPD method using oligo-nucleotide random primers. Genomic DNA isolated from twelve garlic cultivars were amplified by polymerase chain reaction using 143 primers, and 55 primers showed polymorphic DNA bands. Among a total of 187 bands amplified by 55 primers, 128 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. Garlic cultivars were classified into three groups, such as group-I corresponded to Euiseong, Seosan, Samchuk and Yecheon-A, Yechun-B, Euiseong-norang, Jeongsun, Namdo, Yookback and Danyang cultivars, and group-II to Mongolia and group-III to Daeseo cultivars. Thirty DNA bands showing unique specificity to the specific cultivars are likely to be useful for identification of garlic local cultivars as DNA markers.

• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.669-684
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• 2013

This study was investigated to evaluate the effects of biodiversity and pest insect occurrence by soil surface management methods such as green manure crop, grass planting and clean-tilled crops on persimmon orchards. Green manure crop pests was investigated 7 species including Hypera postica, Laodephax striatellus, Megoura crassicauda, Aphis craccivora, Nephotettix cincticeps, Liriomyza sp., black spot and the most dangerous pest was H. postica but it did not damaged persimmon tree. Amount of pests and natural enemies dwelling soil covering plant was not difference between organic farming (hairy vetch planting) and conventional farming (natural weed). Homona magnanima occurred three times a year but it rarely damaged leaf of persimmon. Total adult moth catched by pheromone trap was 1,261 organic farm, 1,003 conventional farm and 621 clean-tilled farm. Grapholita molesta occurred four times a year but it also rarely damaged twig of persimmon. Number of collected invertebrate species and individuals on soil sample was follow as : 838 individuals of 22 species at organic farm, 421 individuals of 17 species at conventional farm and 381 individuals of 15 species at clean-tilled farm. The richness index was lined up by organic farm 3.12> conventional farm 2.65> clean-tilled farm 2.36.

• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.21-26
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• 2015

This study was conducted to compare the aromatic compounds and the internal quality such as total phenolic contents, DPPH activity, and vitamin C contents in two new forma (red type and dalgom) and gom-chwi (Ligularia fischeri). Total phenolic contents were indicated 137.29mg/100g fresh wt. in gom-chwi, 158.40mg/100g fresh wt. in red type, and 180.82mg/100g fresh wt. in dalgom, red type and dalgom had higher contents than gom-chwi. DPPH activities were appeared similar result in gom-chwi (74.45%) and red type (75.59%), and dalgom (86.26%) had better than others. In vitamin C contents, gom-chwi and red variety had shown very closed results but dalgom had best result (1.81mg/100g fresh wt.). Essential oil contents of red type (0.164% of fresh wt.) and dalgom (0.290% of fresh wt.) had higher contents than gom-chwi (0.060%). Analysis of major components of essential oil by GC/MSD, identified 43 compounds in gom-chwi, 44 compounds in red type, and 31 compounds in dalgom. N-containing compound only detected in dalgom. Comparison of six aromatic compounds for fragrance (a-pinene, ${\beta}$-pinene, ${\beta}$-phellandrene, a-phellandrene, 3-carene, and limonene) was indicated red type had higher contents and dalgom had lower contents than gom-chwi. Dalgom had higher contents of total phenolic, DPPH activity, vitamin C, and essential oil than red type and gom-chwi. This result had shown the dalgom had better characters for new cultivar.

• The Korean Journal of Mycology
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• v.25 no.2 s.81
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• pp.111-120
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• 1997

The characteristics of monosporous isolates of winter mushroom [Flammulina velutipes (Curt. ex Fr.)] were investigated to obtain useful breeding materials. Within monokaryons from cultivars which have a white colored fruitbody showed narrow genetic variation, while domestic strains which have a brown colored fruitbody showed wide variation. The mating type of the white strains was A1A2B1B2 genotype, but that of the domestic brown strains were A3A4B3B4. In intra-crossing, the genetic stability of dikaryons mated by monokaryons from white strain was less than that of parents. While in brown strain, dikaryons with high yield and color variation were obtained.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.04a
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• pp.17-37
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• 2001

2000년 6월부터 2000년 10월까지 정읍근교의 정읍 천연림에서 많은 균류를 채집하여 동정하였다. 그 결과 균류다양성은 2문, 3아문, 4강, 14아강, 10목, 31과, 110종을 확인하였다. 그 중에서 미기록종은 이끼귀버섯(Crepidotus epibryus), 황금바퀴버섯(Orbilia xanthostigma)등 이었다. 우점과는 송이과와 광대버섯과였다. 생태적 자원은 식용버섯은 43종, 재배종은 8종, 독버섯은 15종, 약용버섯은 13종, 항암버섯은 15종, 균근형성균은 17종 그리고 목재부후균은 31종이었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.39-39
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• 2021

지구온난화에 따른 기후변화로 과거에는 재배되지 않았던 아열대 작물의 국내재배가 확대되고 있다. 백향과(Passiflora edulis Sims)는 브라질 남부지역이 원산지인 아열대작물로 국내에서도 전국적으로 재배가 되고 있어서, 국내 재배현황과 재배농가의 관리기술 실태를 조사하여 재배 애로사항 및 발전과제를 도출코져 하였다. 백향과의 국내 재배는 2010년부터 시작되어 2017년에는 201농가 54.7ha로 정점을 이루고, 점차 감소하여 2019년에는 156농가 36.5ha에서 재배되고 있다. 재배농가가 많았던 지역은 남원, 담양, 화순, 김천, 고창 등 이었다. 백향과의 재배체계는 대부분의 농가가 비닐하우스 시설에서 겨울철 난방에 의한 다년 재배로 1년 2회 수확하고 있으며, 극히 일부농가는 1년 재배체계로 매년 묘목을 다시 심는 형태이다. 백향과의 국내 육성품종은 없으며, 외국에서 도입한 자색종 또는 교잡종을 주로 재배하고 있다. 현재 국립종자원에 '일반종', '타이농1하오', '황금' 3품종이 생산/수입 판매 신고되어 있다. 초기에 도입한 접목묘를 재배하면서 국내에서 삽목묘를 육성하여 많은 농가가 재배하고 있다. 백향과는 덩굴성이기 때문에 지주를 설치하여 재배하는데 재식거리, 수형, 가지유인 등을 농가별로 다양한 방법으로 하고 있는 실정이다. 개화기에 인공수분 작업이 필요하고, 수확은 과일이 성숙하여 저절로 낙과하면 주어서 수확한 과일은 주로 생과로 판매하는데 전화 및 인터넷 주문에 의한 직거래가 가장 많고, 일부 마트 및 로컬푸드에 판매하고 있다. 국내재배 안전성 향상을 위하여 필요시 되는 기술개발 과제는 적응 품종육성 및 무병묘 생산 보급, 고품질 백향과 생산을 위한 재배관리 기술, 시설재배 환경관리 기술, 소비 증대를 위한 가공 이용 기술 개발 등으로 전문기관에서의 연구개발이 필요하다.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.82-93
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• 2004

Phylogenetic relationships among species in Araliaceae were analyzed using PCR-RAPD and sequence of ITS region of nuclear ribosomal DNA based on samples collected in Korea. RAPD analysis showed various polymorphic bands which were able to differentiate species and genus, and specific bands showing variations among individuals within species. Cluster analysis using gel images revealed high molecular variability within species of Aralia eleta. No significant variation was found among cultivated species of Panax ginseng, but they showed high genetic differences with wild type of the species. In ITS analysis, specific sequences for each genus and species were observed and these were allowed to differentiate species and genus. Phylogenetic analysis using ITS sequences showed that Acanthopanax and Kalopanax had a close relationship, and Aralia and Panax are monophyletic, but genus Hedera is different species from other species in family Araliaceae in this study. The results showing close relationship between genera Aralia and Panax were also observed in RAPD analysis. Contrary to the results of RAPD analysis of Panax ginseng, sequence analysis of ITS showed no significant difference between wild mountain ginseng and cultivated species of P. ginseng. Also, both RAPD and ITS analysis of P. ginseng showed no significant genetic variability among cultivation sites. Results indicate that P. ginseng cultivating in Korea is monophyletic. The molecular analysis used in this study agreed on classification using morphological feature. These results suggest that molecular techniques used in this study could be useful for phylogenetic analysis of Araliaceae.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.72-72
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• 2022

자두나무(Prunus salicina Lindl.)는 세계에서 5번째로 많이 생산되는 과실로, 한국에서 재배하는 자두나무의 기본종은 중국 양쯔강 유역에서 기원했다. 2016년 과수용 자두나무와는 다른 자두나무가 양구에서 발견되었다. 양구군, 인제군, 고성군 일대의 자두나무 개체군의 유전다양성 및 집단 구조 분석을 통해 본 자두나무 개체군의 유전다양성 및 개체군 구조를 확인하고자 했다. 과수용 재배종을 포함한 시료를 채취하여 GBS 분석을 진행했고 주성분 분석과 STRUCTURE 분석을 통해 개체군간 유전적 구조를 확인했다. 재배종의 유전형이 다른 개체군에서도 나타나는 것으로 보아 유의한 유전적 분화가 일어났다고 보기 힘들었다. 하지만 고성군 고진동계곡 등 DMZ에 인접한 집단이 분계도에서 재배종 개체군과 가장 유전적 거리가 있는 것으로 나타났다. 하지만 본 분석으로는 야생집단으로 발견도니 자두나무의 실체와 기원을 단정짓기에는 부족한 것으로 보이며 외군 추가 및 더 많은 시료를 확보하는 등 추가 조사와 분석이 필요하다.

• Food Science and Preservation
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• v.23 no.5
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• pp.727-737
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• 2016

This study was carried out to determine the descriptive sensory profile of oriental melon. The sensory profile of oriental melons (cultivated in Seonju, Kyungsangbuk-do) were used quantitative descriptive analyses and twelve trained panel developed the total forty sensory attributes related appearance, aroma, texture etc. Six appearances attributes and two aromas attributes were derived from whole oriental melon with skin. Five aromas, six tastes and six textures were derived from the mesocarp of oriental melon. And also, oriental melons were analyzed for pH, titratable acidity, solid soluble contents and weight. The weight, titratable acidity and soluble solid content of oriental melons showed significant differences according to samples. And also, in the sensory attributes of these samples by trained panel and consumer test, there were significant differences between organic cultivated samples and conventional cultivated samples. Based on principal component analysis of sensory attributes data by trained panel and consumer, oriental melons were primary separated along the first principal component, which accounted for 27.73% (trained panel test) and 19.82% (consumer test) of total variance (trained panel test; 58.36%, consumer test; 46.18%) between the samples farm, cultivation method, total acidity, sweet aroma, bitterness, etc. Generally, whole oriental melon with skin showed significant differences in the sensory attributes according to cultivation method and farms. But, the mesocarp and endocarp part showed not certainly differences in the sensory attributes between organic cultivated samples except conventional cultivated samples.