• Korean Journal of Food Science and Technology
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• v.44 no.6
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• pp.750-758
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• 2012

This study investigated flavonoid, total phenol, total flavonoid content, antioxidant and antiproliferative activity on human breast cancer cells (MCF-7, MDA-MB-231). Four varieties of Korean green peppers (KP: kkuri pepper, PP: phut pepper, CP: cheongyang pepper, OP: ohi pepper) and one foreign green pepper (JP: jalapeno) were used. The contents of luteolin, quercetin and apigenin, which are abundant flavonoids in green pepper, were the highest in KP. Also, the contents of total phenol, and total flavonoids were the highest in KP, followed by CP, JP, PP, and OP (KP: total phenol $13.29{\pm}0.45$ mg GAE/g D.W., total flavonoid $7.02{\pm}0.13$ mg QE/g D.W. In DPPH ABTS radical-scavenging activity, KP showed the most potent antioxidant activity. In the result of viability in human breast cancer cells, KP had the highest antiproliferative effect. These results suggest that green peppers have significant antioxidant activity and can be a possible candidate for treatment of breast cancer.

• Journal of Life Science
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• v.30 no.10
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• pp.896-904
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• 2020

The purpose of this study was to investigate the probiotic characteristics and immune activities of selected lactic acid bacterial (LAB) strains as feed additives in livestock. 301 LAB strains isolated from traditional fermented foods were first assessed for their antibacterial activity potential. Of the 301 isolates, five showed antibacterial activity against five livestock pathogens (Esherichia coli KCCM11234, Listeria monocytogens KCTC3710, Salmonella Typhimurium KCTC1926, Staphylococcus aureus KCCM11593, and Shigella flexneri KCTC2517). The probiotic characteristics of the five selected strains were also investigated by antioxidative activity, hemolysis, bile salt hydrolase, acid resistance and bile tolerance. The SRCM102607 strain was found to have superior probiotic properties and was selected for further experimentation. 16S rRNA gene sequencing showed that SRCM102607 is Pediococcus acidilactici, which was labeled as P. acidilactici SRCM102607 (KCCM 12246P). The survival characteristics of P. acidilactici SRCM102607 in artificial gastrointestinal conditions were assessed under exposed acidic (pH 2.0) and bile (0.5% and 1.0%) conditions. P. acidilactici SRCM102607 was also confirmed to have resistance to various antibiotics, including amikacin, gentamicin, vancomycin, and etc. The TNF-α production by P. acidilactici SRCM102607 was 171.86±4.00 ng/ml. These results show that P. acidilactici RCM102607 has excellent potential for use as a probiotic livestock feed additive.

• Microbiology and Biotechnology Letters
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• v.50 no.4
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• pp.557-562
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• 2022

In this study we evaluated biohydrogen production potential as operational parameters (substrate, salt concentration, and temperature) using eight inoculum sources. While the volumetric biohydrogen production rate was significantly affected by temperature and inoculum sources, substrate and salt concentration did not have a significant effect on the biohydrogen production. Mesophilic temperature (37℃) was also found more appropriate for the hydrogen production than thermophilic temperature (50℃). Rate, while the eight inoculum sources, anaerobic digestion sludge exhibited the fastest biohydrogen production. The maximum production rate from anaerobic digestion sludge was 2,729 and 1,385 ml-H₂·l^-1·d^-1 at mesophilic and thermophilic temperature, respectively.

• Korean Journal of Organic Agriculture
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• v.20 no.2
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• pp.173-183
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• 2012

This study was conducted to select promising green manure crops, providing sufficient amount of nutrients for satisfying fruit tree growth, with the overwintering cover crops grown in organic orchards in 2009. The cover covers were investigated in 13 organic orchards in Chonnam province in April and June. The dry matter in cover crops observed in April and June was the highest for Lolium multiflorum Lam. and Bromus japonicus Thunb., respectively. Total N and K production in April was the highest for Lolium multiflorum Lam, Vicia hirsuta (L.) S. F. Gray and Vicia angustifolia var. segetilis (Thuill.) K. Koch., respectively, with Bromus japonicus Thunb. in June. This study showed that the leguminous crops, Vicia hirsuta (L.) and Vicia angustifolia, would be the prospective cover covers as the both crops provided sufficient amount of N and $K_2O$ into the soil. Amount of $P_2O_5$ producing from all cover crops provided less than nutrient levels than those of recommended nutrient requirement for satisfying 10- to 15-year-old fruit tree growth.

• Journal of The Korean Society of Agricultural Engineers
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• v.58 no.2
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• pp.65-71
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• 2016

The new concept of agricultural water supply potential, which is mean annual turnover rate times unit storage capacity, was introduced for agricultural reservoirs. We investigated characteristics of mean annual turnover rate and unit storage capacity for agricultural reservoirs with storage capacity of over $1million\;m^3$. The curve of agricultural water supply potential represents change in mean annul turnover rate according to change in unit storage capacity. The mean annual turnover rate and unit storage capacity in the reservoirs with high minimum storage ratio are significantly higher than those in the reservoirs with low minimum storage ratio. Most of unstable water supply reservoirs showed low mean annual turnover rate or low unit storage capacity, indicating that mean annual turnover rate may be an index of stability degree for agricultural water use. The reservoirs with mean annual turnover rate of over 2 and unit storage capacity of over 0.8 m may be estimated as the stable water supply zone for 10 frequency dry year. The reservoirs with high agricultural water supply potential can belong to the wide range of stable water supply zone. The results suggest that relation between mean annual turnover rate and unit storage capacity may be used in evaluating stability degree for agricultural water supply in the reservoirs.

• Journal of Life Science
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• v.17 no.11
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• pp.1582-1586
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• 2007

Previous study (J. of Chem. Technol. Biotechnol. 2004, 79, 79-84) showed that bacterial cellulose (BC) produced by a bacterial strain JH232 has potential as a source for environmentally friendly ion exchange membranes. In this study, strain JH232 was investigated for phylogenetic classified and characterized for BC production. Comparative analysis of 16S rRNA gene revealed that the strain belongs to the genus Gluconacetobacter. Maximum production of BC was observed when JH232 was cultured in CSL medium (pH 5.5) at $30^{\circ}C$ as determined by flask experiment. When batch and fed-batch cultures of JH232 were performed in the fermenter experiment to compare BC productivity of the strain, BC productivity of fed-batch culture was 1.56 times higher than that of batch culture.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.59-59
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• 2021

최근 기후변화에 따른 가뭄의 상습화와 농촌용수 수요증가에 대비할 수 있는 가뭄대책의 수립을 통한 용수부족 문제에 대한 근본적인 해소의 필요성 증가하고 있는 가운데 기존 수자원을 효율적으로 활용하기 위한 4차 산업기술을 적용한 순환형 농업용수 관리시스템의 개발이 요구되고 있다. 본 연구는 2017년 가뭄이 극심하였던 충청남도 대사저수지, 풍전저수지와 경기도 마둔저수지에 대하여 기상분석, 관개분석, 수리분석 등을 수행하여 이수관리, 관개만족도, 회귀수의 재이용 등을 예측 관리할 수 있는 순환형 농업용수 관리 시스템을 개발하여 적용하고자, 선제적으로 가뭄 발생 들녘의 용수공급을 위한 농업용 저수지 소유역 순환용수 가용수량을 추정하였다. 2010년부터 2019년까지의 강우유출 모형을 적용하기 위하여 수원, 천안, 이천관측소의 강우, 최고/최저기온, 상대습도, 풍속, 일사량자료를 일자료로 활용하였고, 10년 평균 유출량을 순별로 산정하여 기본자료로 구축하여 주요 취입보 유입수량을 산정하였다. 주요 취입보 유입수량 산정과 취입보의 용적을 산출하기 위하여 시설별 높이차와 하천의 길이 및 폭을 측정하였으며 물수지는 보유용수량과 유입량의 합에서 공급수량을 제외한 값으로 정의하였고, 내용적을 초과하는 경우에는 범람되는 것으로 구현하여 마둔, 대사, 풍전저수지의 임의 지점(하천)별로 순환용수 가용을 위한 모의를 실시하였다. 공간 범위는 평야부 말단부로 선정하였고, 가상으로 약 2m 높이의 임시보를 적용하여 관개기 강우유출수 및 퇴수에 의한 가용수량을 산정하였다. 이를 위하여 하천의 폭과 표고를 이용하여 길이를 산출하여, 내용적을 산출하였다. 소유역별로 TANK 모형적용 결과를 입력자료로 활용하여 일 공급 양수량을 내용적 최대로 하였을 때 회복 기간에 대한 모의를 수행하였다. 강우유출수에 의한 소유역별 가용수량을 4월부터 모의 분석한 결과 유역별로 차이는 발생하지만 만수위에 도달하는 기간은 특이 유역을 제외하고는 약 50일 이상 걸리는 것으로 분석되었고, 관개기가 계속되면서 회복되는 기간이 짧아지는 경향을 보였다. 이를 통해 소유역별 농업용수 부족분 투입을 위한 위치선정과 용수량 판단이 가능할 것으로 예상된다.

• Journal of the Korean Society of Radiology
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• v.84 no.3
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• pp.520-535
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• 2023

Gastrointestinal (GI) bleeding is not a single disease but a symptom and clinical manifestation of a broad spectrum of conditions in the GI tract. According to its clinical presentation, GI bleeding can be classified into overt, occult, and obscure types. Additionally, it can be divided into upper and lower GI bleeding based on the Treitz ligament. Variable disease entities, including vascular lesions, polyps, neoplasms, inflammation such as Crohn's disease, and heterotopic pancreatic or gastric tissue, can cause GI bleeding. CT and conventional angiographies and nuclear scintigraphy are all radiologic imaging modalities that can be used to evaluate overt bleeding. For the work-up of occult GI bleeding, CT enterography (CTE) can be the first imaging modality. For CTE, an adequate bowel distention is critical for obtaining acceptable diagnostic performance as well as minimizing false positives and negatives. Meckel's scintigraphy can be complementarily useful in cases where the diagnosis of CTE is suboptimal. For the evaluation of obscured GI bleeding, various imaging modalities can be used based on clinical status and providers' preferences.

• Food Science and Preservation
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• v.23 no.3
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• pp.432-437
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• 2016

The objective of this research was to investigate the antioxidant and antimicrobial activities of Jeok Hasuo (Polygonum multiflorum Thunb., PM) and Baek Hasuo (Cynanchi wilfordii Radix, CW) root extracts. Total phenolic contents of PM and CW root extracts were determined and the antioxidant activities of the root extracts were determined by scavenging activity of diphenylpicrylhydrazyl (DPPH) radicals. The antimicrobial activities against Staphylococcus aureus and Propionibacterium acnes were determined and expressed as the minimum inhibitory concentration (MIC). The disc diffusion method was also used to determine the zone of inhibition. The butanol extracts of PM and CW roots exhibited greater total phenolic contents (1,212.6 and 1,454.5 mg/g GAE, respectively) than those of ethanol and water extracts. The ethanol (89.0%) and butanol extracts (88.9%) of PM exhibited significantly greater DPPH radical scavenging activities than that of water extracts (73.1%) (p<0.05). Only ethanol extract exhibited an MIC of 0.8 mg/mL against both bacteria. Zones of inhibition started to form when the concentration of extract was greater than 5 mg/disc. The diameters of the zone of inhibition of PM and CW were measured to be 8.9 and 9.2 mm against S. aureus and P. acnes, respectively, exhibiting the greatest antimicrobial activities among the extracts. This research demonstrated that the PM and CW root extracts possessed not only antioxidant activity but also strong antimicrobial activity against skin-related bacteria.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.357-366
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• 2015

Quorum sensing (QS) is involved in the process of cell-to-cell communication and as a gene regulatory mechanism, which has been implicated in bacterial pathogenicity. Bacteria use this QS system to control a variety of physiological processes. In this study, pomegranate (Punica granatum L.) peel extract (PPE) was first screened for its ability to inhibit QS in bio-reporter strains (Chromobacterium violaceum and C. violaceum CV026). Next, the ability of PPE to inhibit swimming motility and biofilm formation was examined in Y. enterocolitica. Additionally, changes in the expression of specific genes involved in the synthesis of the N-acylhomoserine lactones (AHLs; yenI and yenR) and in the flagellar regulon (fliA, fleB and flhDC) were evaluated by reverse transcription (RT)-PCR. The results show that PPE specifically inhibited and reduced QS-controlled violacein production by 78.5% in C. violaceum CV026, and decreased QS-associated biofilm formation and swimming motility in Y. enterocolitica without significantly affecting bacterial growth. These inhibitory effects were also associated with the down-regulation of gene expression involved in the synthesis of AHLs and in motility. Our results suggest that PPE could be a potential therapeutic agent to prevent enteropathogens in humans, as well as highlight the need to further investigate the in vivo properties of PPE for clinical applications.