• The Korean Journal of Ecology
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• v.23 no.5
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• pp.353-357
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• 2000

The soil pH favored by several native plants in Korea ranges 5.33∼7.20, while a more acidic range of pH 3.95∼6.10 is acceptable to exotic plants. Ethanol extracts of native and exotic plants in Korea were investigated for antimicrobial activity against Bacillus sphiaericus 2362, Bacillus thuringiensis var. subtilis and Bacillus thuringiensis var. cereus and Actinomycetes. Higher antimicrobial activity was observed from the extract of exotic plants than those of native plants. The ethanol extract of Ambrosia artemisiifolia var. elatior was observed to have the highest antimicrobial activity against 4 species of soil microbes. Especially, antimicrobial activity of Ambrosia artemisiifolia var. elatior showed the largest clear zone of 48mm in Actinomycetes. Larger clear zone was formed in the order of caffeic acid, benzoic acid and ρ -coumaric acid among the nine chemical compounds. Accordingly, the antimicrobial activity of Ambrosia artemisiifolia var elatior against Actinomycetes was found to be due to the synergetic effect of chemical compounds.

• Proceedings of the EASDL Conference
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• 2003.09a
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• pp.85-85
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• 2003

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2002.08a
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• pp.187.1-187
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• 2002

• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.210-219
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• 2007

In this study was investigated the growth effect of native plant (Microlepia marginata(panzer Christ., Prunella vulgaris. aleutica Fernald, Perillafrutescens var. japonica Hara., Gleichenia japonica Spreng) extracts on various lactic acid bacterias, electron donating ability, total cell count and hydroxyl radical scavenging activity. The total cell count of Enterococcus faecium KCCM 12118, Lactobacillus rhamnosus KCCM 32826, Lactobacillus plantarum KCCM 11542, Pediococcus pentosaceus KCCM 40464 in the absence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $2.2{\times}10^9\;cfu/ml$, $2.1{\times}10^9\;cfu/ml$, $2.3{\times}10^9\;cfu/ml$, $2.2{\times}10^9\;cfu/ml$. On the other hand, the total cell count of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 in the presence of native plant extracts (10%) at $37^{\circ}C$ after 48hr were $4.3{\times}10^9\;cfu/ml$, $4.3{\times}10^9\;cfu/ml$, $4.8{\times}10^9\;cfu/ml$, $3.9{\times}10^9\;cfu/ml$. The electron donating ability indicated to E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 added by 10% native plant extracts, respectively. when 10% native plant extracts were added lactic acid bacterias, the electron donating ability is the highest. Hydroxyl radical scavenging activity of E. faecium KCCM 12118, L. rhamnosus KCCM 32826, L. plantarum KCCM 11542, P. pentosaceus KCCM 40464 showed higher than that of control.

• The Korean Journal of Ecology
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• v.22 no.1
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• pp.51-58
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• 1999

Korean native plants and naturalized plants were analyzed for allelochemicals, and their antimicrobial effects were studied. The difference in soluble solid contents between Korean native plants and naturalized species was not significant, and the Korean native plant, Solanum nigrum showed the highest soluble solid content of 90 mg/ml. The ethanol extract of the Korean native plant, Solanum nigrum showed antifungal activity to Aspergillus phoenicis KCTC 1228, with a clear zone of 18 mm, and spore formation was not observed from the treatment. The naturalized plants Ambrosia artemisiifolia var. elatior and Erigeron canadensis showed inhibition of spore formation and the clear zones were at 24 mm and 22 mm, respectively. The clear zones of Aspergillus phoenicis KCTC 1228 treated with ethanol extrats of Phytolacca americana and Rudbeckia bicolor were 22 mm and 19 mm, respectively, and spore formation was observed from the treatment. The Korean native plant, Solanum nigrum and naturalized plants, Phytolacca americana and Ambrosia artemisiifolia var. elatior showed antimicrobial activity against Bacillus sphiaericus 2362, and Bacillus sphiaericus 2297, Bacillus thuringiensis var. subtilis and Baicillus thuringiensis var. cereus. The antimicrobial activity of Ambrosia artemisiifolia var. elatior showed the largest clear zone of 32 mm against Bacillus thuringiensis var. subtilis. In general, the more soluble the solid contents of the extracts, the greater were the antifungal and antimicrobial activities. The phenolic compounds from the Korean native plant, Solanum nigrum and the naturalized species, Phytolacca americana and Ambrosia artemisiifolia var. elatior were analyzed by high performance liquid chromatography. Three phenolic compounds including hydroquinone were identified in Solanum nigrum. In contrast, five and seven phenolic compounds were identified in Phytolacca americana and Ambrosia artemisiifolia var. elatior, respectively. The antifungal activity against Aspergillus phoenicis KCTC 1228 was found to be due to the coumaric and benzoic compounds.

• Journal of Mushroom
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• v.22 no.1
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• pp.22-26
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• 2024

Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called "shiro," which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.

• Journal of Soil and Groundwater Environment
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• v.11 no.5
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• pp.51-58
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• 2006

In this study, uptake and toxicity of disel (TPH) by poplar specie, $P.\;nigra{\times}P.\;maximowiczii$ were assessed in laboratory soil column experiments. Poplar cuttings were grown for 2 months and exposed to various concentration (0, 200, 500, 1000, 2000 mg/kg) of disel for a period of 60 days. For disel removal experiments, disel was effectively removed in the range of lower concentration. but, the removal rate of disel was rapidly decreased as increasing initial disel concentrations. For the this reason, toxicity effetcs were evaluated by measuring in poplar cutting mass variation and monitoring transpiration. Exposure on higher disel concentration resulted in decrease of biomass and transpiration accompanied by chlorosis and abscission, indicating toxic effect of disel on the poplar tree. And also, we have observed that both removal efficiency of disel and the microbial activity were higher at the bottom of the soil column. It was suggested that the plant formed the root zone at contaminated soil, stimulated microbial activity by plant root exudates, and played an important role in enhanced biodegradation of disel.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.399-422
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• 2020

Remediating soils contaminated with heavy metals due to urbanization and industrialization is very important not only for human health but also for ecosystem sustainability. Of the available remediation technologies for heavy metal-contaminated soils, phytoremediation is a relatively low-cost environment-friendly technology which preserves biodiversity and soil fertility. The application of plant growth-promoting bacteria (PGPB) during the phytoremediation of heavy metal-contaminated soils can enhance plant growth against heavy metal toxicity and increase heavy metal removal efficiency. In this study, the sources of heavy metals that have adverse effects on microorganisms, plants, and humans, and the plant growth-promoting traits of PGPB are addressed and the research trends of PGPB-assisted phytoremediation over the last 10 years are summarized. In addition, the effects of environmental factors and PGPB inoculation methods on the performance of PGPB-assisted phytoremediation are discussed. For the innovation of PGPB-assisted phytoremediation, it is necessary to understand the behavior of PGPB and the interactions among plant, PGPB, and indigenous microorganisms in the field.

• Korean Journal of Microbiology
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• v.43 no.3
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• pp.201-209
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• 2007

The CFDA (6-carboxyfluorescein diacetate) direct viable count method and plate count (PC) method using conventional nutrient broth (NB) medium and $10^{-2}$ diluted NB (DNB) medium were applied to samples collected from Mt. Yongdoo In Andong, in an effect to determine the number of living bacteria pine mushroom forest soil. The number of living bacteria determined via plate count in NB medium comprised $5{\sim}8%$ of the CFDA direct viable count, and the bacteria in the DNB medium comprised $40{\sim}47%$. This result indicated that viable but nonculturable (VBNC) bacteria existed in the pine mushroom forest soil at a high percentage. The phylogenetic characteristics of the VBNC bacterial populations in the samples of pine mushroom (Tricholoma matsutake) forest soil were analyzed via the direct extraction of DNA and 16S rDNA-ARDRA. The 115 clones from pine mushroom forest soil were clustered into 31 different RFLP phylotypes by ARDRA. Based on the 16S rDNA sequences, the 31 ARDRA clusters were classified into 6 phylogenetic groups: ${\alpha}-,\;{\beta}-,\;{\gamma}-Proteobacteria$, Acidobacteria, Actinobacteria and Firmicutes. Among these bacterial populations, approximately 85% were classified as members of phylum Acidobacteria. The Acidobacteria phylum was shown to exist abundantly in the pine mushroom forest soil.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.59-66
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• 2018

Propionibacterium acnes, a major pathogens bacterium of acne, is one of the microflora associated with the inflammation. In this study, the antibacterial effects of 70% ethanol extracts from the 61 natural plants in Jeju against P. acnes were investigated using the disc diffusion method. The 45 natural plant extracts showed antibacterial effects and the 16 natural plants extracts didn't show antibacterial effects. The Phyllanthus urinaria L. (stem and leaves) extract showed thr highest antimicrobial activity with $18.96{\pm}0.69mm$ clear zone of the growth of P. acnes, followed by Angelica gigas Nakai (roots), Gynostemma pentaphyllum (Thunb.) Makino, Angelica gigas Nakai (stem and leaves), Isodon inflexus (Thunb.) Kudo, camellia sinensis L. (flowers), Rosmarinus officinalis, etc. These results suggest that the natural plant in Jeju can be used as functional cosmetic biomaterials or antimicrobial agents against P. acnes.