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Characterization of Burcucumber Biochar and its Potential as an Adsorbent for Veterinary Antibiotics in Water (가시박 유래 바이오차의 특성 및 항생물질 흡착제로서의 활용가능성 평가)

  • Lim, Jung Eun;Kim, Hae Won;Jeong, Se Hee;Lee, Sang Soo;Yang, Jae E;Kim, Kye Hoon;Ok, Yong Sik
    • Journal of Applied Biological Chemistry
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    • v.57 no.1
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    • pp.65-72
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    • 2014
  • Biochar (BC) from biomass pyrolysis is a carbonaceous material that has been used to remove various contaminants in the environment. The eliminatory action for burcucumber (Sicyos angulatus L.) as an invasive plant is being consistently carried out because of its harmfulness and ecosystem disturbance. In this study, burcucumber biomass was converted into BCs at different pyrolysis temperatures of 300 and $700^{\circ}C$ under a limited oxygen condition. Produced BCs were characterized and investigated to ensure its efficiency on antibiotics' removal in water. The adsorption experiment was performed using two different types of antibiotics, tetracycline (TC) and sulfamethazine (SMZ). For the BC pyrolyzed at a high temperature ($700^{\circ}C$), the values of pH, electrical conductivity, and the contents of ash and carbon increased whereas the yield, mobile matter, molar ratios of H/C and O/C, and functional groups decreased. Results showed that the efficiency of BCs on antibiotics' removal increased as pyrolysis temperature increased from 300 to $700^{\circ}C$ (38 to 99% for TC and 6 to 35% for SMZ). The reaction of ${\pi}-{\pi}$ EDA (electron-donor-acceptor) might be involved in antibiotics' adsorption to BCs. BC has potential to be a superior antibiotics' adsorbent with environmental benefit by recycling of waste/invasive biomass.

Deodorization of H2S, CH3SH in Soil Filter Reactors Packed with Montmorillonites, Rice Hulls and Thickening-activated Sludge (Montmorillonites, 왕겨 및 농축활성슬러지를 충진한 토양상에서의 H2S, CH3SH의 제거)

  • Kim, Hwan-Gi;Park, Chan-Soo
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.1
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    • pp.43-52
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    • 2000
  • Deodorization characteristics and removal rate of sulfur-containing odor have been investigated in the soil filter reactors packed with montmorillonites (Mont.), rice hulls(Rh.), and thickening-activated sludge(Ts.). And variation of pH and $SO_4{^{2-}}$ with the removal of malodorous sulfur compounds have been investigated together. As compared removal rate of montmorillonites between wet and dry condition for sulfur compounds through batch test, it showed that wet condition was better than dry one; removal ratio, as wet/dry, was $H_2S$ of 1.2 and $CH_3SH$ of 1.9, and decrease of pH and increase of $SO_4{^{2-}}$ concentration in the wet condition also showed to be larger than in dry condition. In continuous test for biological deodorization experiment, removal rate of sulfur compounds in reactor packed with Mont., Rh. and Ts, was more than 98 %, and the variation of static pressure was maintained stably under condition of SV $150h^{-1}$, LV 4.2 mm/sec and SV $200h^{-1}$, LV 5.6 mm/sec, and in reactor packed with Mont. and Rh., $H_2S$ was 76.4 % to 87.2 % and $CH_3SH$ was 87.8 % to 93.3 % under the same condition. From above results, it ascertained that it can obtain the high deodorization efficiency by inoculating thickening-activated sludge in soil filter using montmorillonites.

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Effect of Cover Crop Hairy Vetch on Prevention of Soil Erosion and Reduction of Nitrogen Fertilization in Sloped Upland (경사지 밭토양 유실억제 및 질소비료절감에 대한 피복작물 헤어리벳치의 효과)

  • Seo, Jong-Ho;Park, Jong-Yeol;Song, Duk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.3
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    • pp.134-141
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    • 2005
  • Cultivation of winter cover crops and its soil utilization for summer main crops in slope upland is very important at the respects of diminution of soil erosion and non-point pollution source. The effects of cover crop, hairy vetch as no-tillage or conventional tillage on prevention of soil erosion and reduction of chemical nitrogen fertilization were investigated in the slope upland with whiter clover living mulch (partial tillage) in Suwon for three years and with rye (conventional tillage) in Hongcheon for two years, respectively. In Suwon, amounts of soil lost by rainfall runoff decreased as much as 90% by hairy vetch-no tillage (HV-NT), white clover-partial tillage (WF-PT) together with the decrease of rainfall runoff compared to winter fallow with conventional tillage (WF-CT). In addition, amounts of weed also decreased as much as 80-90% by HV-NT and WF-PT. Corn yield decreased much at the plot of WF-PT mainly due to competition for soil water and nutrients between clover and corn at the early corn growth stage. On the contrary, corn yield increased by HV-NT compared to WF-CT regardless of weed control. In Hongcheon, amounts of soil eroded during winter season before corn seeding were reduced as much as 95% by cultivation of hairy vetch and rye compared to winter fallow. Amount of soil eroded during waxy corn growing season was reduced as much as 98% by HV-NT compared to WF-CT. Also, soil incorporation of hairy vetch and rye as green manure with conventional tillage at corn seeding time could reduce soil erosion as much as 70% compared to no soil cover with conventional tillage. Ear yields of waxy corn were increased 10% higher at hairy vetch green manure (HV-CT) without nitrogen fertilizer, 20% higher at HV-NT with standard nitrogen fertilizer, respectively than WF-CT. But ear yields of waxy corn were decreased by rye green manure (R-CT) and HV-NT at the condition of no nitrogen fertilizer. It was concluded that hairy vetch was better as winter cover crop to reduce both soil erosion and chemical nitrogen fertilizer simultaneously in slope upland than other cover crops.

Eco-friendly remediation and reuse for coastal dredged materials using a bioaugmentation technology (생물증강법을 이용한 오염해양준설토의 환경친화적 정화 및 재활용)

  • Kim, In-Soo;Ha, Shin-Young;Koh, Sung-Cheol
    • Korean Journal of Microbiology
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    • v.51 no.4
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    • pp.374-381
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    • 2015
  • Occurrences of coastal dredged materials are ever increasing due to port construction, navigational course maintenance and dredging of polluted coastal sediments. Ocean dumping of the coastal dredged materials has become virtually prohibited as London Treaty will be enacted as of the year 2012. It will be necessary to treat and recycle the dredged materials that may carry organic pollutants and heavy metals in a reasonable and effective process: collection of the dredged materials, liquid and solid separation, and treatment of organic compounds and heavy metals. In this study we have developed a continuous bioreactor system that can treat a mixture of silt and particulate organic matter using a microbial consortium (BM-S-1). The steady-state operation conditions were: pH (7.4-7.5), temperature ($16^{\circ}C$), DO (7.5-7.9), and salt concentration (3.4-3.7%). The treatment efficiencies of SCOD, T-N and T-P of the mixture were 95-96%, 92-99%, and 79-97%. The system was also effective in removal of heavy metals such as Zn, Ni, and Cr. Levels of MLSS during three months operation period were 11,000-19,000 mg/L. Interestingly, there was little sludge generated during this period of operation. The augmented microbial consortium seemed to be quite active in the removal of the organic component (30%) present in the dredged material in association with indigenous bacteria. The dominant phyla in the treatment processes were Proteobacteria and Bacteroidetes while dominant genii were Marinobacterium, Flaviramulus, Formosa, Alteromonadaceae_uc, Flavobacteriaceae_uc. These results will contribute to a development of a successful bioremediation technology for various coastal and river sediments with a high content of organic matter, inorganic nutrients and heavy metals, leading to a successful reuse of the polluted dredged sediments.

Effects of No-Tillage on Pepper Growing in the Greenhouse with Organic Soil (시설 고추 무경운 유기재배 효과)

  • Yang, Seung-Koo;Seo, Youn-Won;Kim, Hyun-Woo;Kim, Byeong-Ho;Kim, Yong-Sun;Im, Gyeong-Ho;Kim, Hong-Jae;Kim, Jeong-Geun
    • Proceedings of the Korean Society of Organic Agriculture Conference
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    • 2009.12a
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    • pp.287-287
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    • 2009
  • 우리나라의 시설재배면적은 51,237ha에 이르며 수경재배 면적을 제외하면 대부분 경운재배를 하고 있는 실정이다. 경운 작업은 대형 농기구를 이용하여 경운하기 때문에 시설내 토양 입자가 단립으로 잘게 부서지며 대형농기구로 인하여 토양이 답압되고 경운층 아래 경반층이 형성되어 뿌리 발육과 배수 불량의 원인을 제공하게 된다. 경운은 기존에 설치되어 있는 관수시설과 지주, 멀칭을 제거한 후 유기물을 투입하고 2~3회 경운작업 후 다시 소형농기구를 이용하여 이랑을 조성한다. 그리고 제거된 관수시설, 멀칭, 지주를 다시 설치하여야 하기 때문에 시설의 경운재배는 가장 고통스러운 악성노동을 수반하게 된다. 따라서 본 시험에서는 시설 고추 유기재배 시 무경운재배 효과에 대하여 검토하고자 전남 나주시 남평읍 평산리 "JD 중동통 미사질양토"에서 시험을 수행하였다. 2009년 3월 11일 "녹광" 고추 품종을 재식 거리 135${\times}$38cm로 정식하여 7월 27일까지 재배한 후, 시험포를 1/2로 나누어 트렉터를 이용하여 경운작업을 실시하고 2009년 8월 1일 억제작형으로 "녹광" 고추 품종을 정식하였다. 그리고 나머지 시험포 1/2은 전작물을 재배한 이랑에 경운작업을 생략하고 전작물 고추의 식물체는 지제부를 절단하여 분해되도록 고랑을 피복하였으며, 고추 포기와 포기 사이에 무경운으로 고추를 정식하여 시험을 수행하였다. 고추 정식 전 7월 30일에 경운 시험포에 10a당 29톤의 물을 관수하였으며, 무경운 시험포에는 10a당 48톤의 물을 관수하고, 관수 2일 후 시험포의 습도와 이랑에 발생되는 균열수를 조사하였다. 이랑의 수분함량은 표토 0.23%, 토양 30cm 깊이의 수분함량은 0.18%로 경운방법에 따라서 차이가 없었으나 토양 20cm 깊이의 수분함량은 경운은 0.25%, 무경운은 0.14%로 경운재배 토양에 비하여 뿌리의 주요 분포지역인 작토층의 수분함량이 44% 정도 낮았다. 그리고 경운재배 이랑에서는 7~35cm 정도 되는 균열이 m당 22개정도 발생되었으나 무경운에서는 관찰되지 않았다. 시설 토양의 생물다양성 지표가 되는 미소동물의 분포를 조사한(9월 16일) 결과 경운재배 토양에서는 보리톡톡기 등 8종 97개체가, 무경운 토양에서는 10종 101개체가 포획되었다. 경운 방법에 따른 시설 유기재배 고추의 수확과수는 주당 139~141과, 수량은 1,367~1,431g/주가 수확되고 10a당 수량은 경운재배가 2,790kg, 무경운재배는 2,666kg이 생산되어 경운방법에 따른 수량 차는 인정되지 않았다. 경운방법에 따른 경영비를 분석한 결과 무경운은 경운재배에 비하여 10a당 유기질 비료비 178천원, 대농구상각비 211천원, 노력비 261천원의 절감으로 경운재배 경영비 4,815천원/10a 대비 약 14%의 경영비가 절감되었다. 그리고 작기마다 반복되는 경운작업과 관수시설, 멀칭, 지주설치 작업을 1회 설치 작업으로 연속사용이 가능하여 시설재배에서 가장 고통스러운 악성노동의 회피가 가능하였다.

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Cross-sectional Changes of Ridge Traversing Trail in Jirisan National Park (지리산국립공원 종주등산로의 횡단면 변화 - 노고단~삼도봉 구간을 중심으로 -)

  • Kim, Taeho;Lee, Seungwook
    • Journal of the Korean association of regional geographers
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    • v.19 no.2
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    • pp.234-245
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    • 2013
  • In order to examine the amount and rate of soil erosion on Ridge Traversing Trail in Jirisan National Park, a cross-sectional area of hiking trail were monitored at 16 sites in Nogodan - Samdobong section from November 2011 to April 2012. Although all sites demonstrates an enlarged cross-section of trail, the amount of soil erosion varies from site to site: 54.9 to $908.8cm^2$. It suggests that the erosional rate ranges from $0.1cm^2/day$ to $1.72cm^2/day$. The erosional amount is also varied with a trail type: $109.3cm^2$ for a shallow gully-like trail to $573.2cm^2$ for a unilateral trail. However, the cross-sectional change is larger on a sidewall than a tread irrespective of a trail type. The erosional amounts of November to April are smaller than that of May to October. In particular, the erosional amount of November 2011 to April 2012 is smaller than the depositional amount, implying a reduced cross-section of trail. Pipkrake action puts loose soil particles on a sidewall on March and April, and then rainwash due to a heavy rainfall takes them away after May. It seems to be the most predominant erosional process in Ridge Traversing Trail. A sidewall facing north shows a larger amount of erosion than a sidewall facing south. It also implies a difference in the development of a pipkrake according to an aspect. The small amount of erosion and cross-sectional decrease, which is usually observed on April, results from the combined effect of frost heaving, pipkrake action, a small rainfall and a temporary suspension of trampling. It is necessary to establish the monitoring system of trail erosion in terms of the management of hiking trail in a mountain national park.

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Cytotoxicity of Particulate Matter in Various Human Cells Lines (미세먼지가 다양한 사람 세포주에 미치는 세포 독성)

  • Lee, Ji-Hyeon;Lee, Joo-Yeong;Kim, Mi-Jeong;Kim, Hyeon-Ji;Lee, Sung-Ho;Jeon, Byeong-Gyun
    • Journal of Life Science
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    • v.29 no.6
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    • pp.724-734
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    • 2019
  • The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.