• Title/Summary/Keyword: 일반성분분석

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Optimization of Medium to Improve Protease Production Using Response Surface Methodology by Bacillus amyloliquefaciens SRCM115785 (반응표면분석법을 이용한 Bacillus amyloliquefaciens SRCM115785의 protease 활성증가를 위한 배지 최적화)

  • Yang, Hee Gun;Ha, Gwangsu;Ryu, Myeong Seon;Park, Se Won;Jeong, Ho Jin;Yang, Hee-Jong;Jeong, Do-Youn
    • Journal of Life Science
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    • v.31 no.8
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    • pp.761-770
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    • 2021
  • In this study, the optimal medium composition for enhancing protease production was established by the Bacillus strain isolated from Makgeolli, a traditional fermented food, using the response surface methodology. B. amyloliquefaciens SRCM115785 was selected as the protease producer by productivity analysis and identified by 16S rRNA gene sequencing. Plackett-Burman design (PBD) was introduced to analyze the effect of each component on protease production among the 11 selected medium components. As a result, glucose, yeast extract, and beef extract were finally selected as factors for enhancing protease production. Central composite design (CCD) analysis was designed as a method to determine the optimal concentration of each component for protease production and the concentration of each medium composition for maximum protease production was predicted to glucose 6.75 g/l, yeast extract 12.42 g/l and beef extract 17.48 g/l. The suitability of the experimental model was proved using ANOVA analysis and as a result of quantitative analysis to prove this, the amount of increase was 230.47% compared to the LB medium used as a control. Through this study, the optimization of medium composition for enhancing protease production was established, and based on this, it is expected that it can be efficient use of protease as an industrial enzyme.

Extractive Nitrogenous Constituents in the Commercial Marine Seasoned-dried Products and Seasoned-dried and Roasted Products (시판 수산조미건제품의 함질소 엑스성분 조성)

  • Park, Choon-Kyu;Park, Jung-Nim
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.370-379
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    • 2002
  • The composition of extractive nitrogenous constituents in four species of commercial marine seasoned-dried products (SDP) and five species of seasoned-dried and roasted products (SDRP) were analyzed. The extractive nitrogen contents were $688{\sim}1,124$ and $756{\sim}1,099\;mg/100g$ in SDP and SDRP extracts. Contents of Free amino acids such as glutamic acid, taurine, arginine, proline, alanine, and histidine were high in all samples. The combined amino acid amounted to $662{\sim}2,248$ and $1,146{\sim}1,821\;mg/100g$ in SDP and SDRP, respectively, which corresponded to 34.5 and 42.5% of the total free amino acid level. ATP and related compounds were $3.69{\sim}7.37$ and $2.17{\sim}8.45\;{\mu}mol/g$ in SDP and SDRP, respectively. Five types of betaines were detected in both specimens although in small amounts. TMAO, TMA, creatine, and creatinine were detected in both samples, however they have same variation in each sample. There was no significant difference in the extractive nitrogenous constituents between SDP and SDRP except in moisture, salinity, and contents of glutamic acid and creatinin (p<0.01).

Analysis of Chemical Composition and Antioxidant Activity of Codonopsis lanceolata Skin (더덕껍질의 일반성분 분석과 항산화 활성)

  • Kim, Na-Young;Chae, Hyun-Seok;Lee, In-Sook;Kim, Dong-Soo;Seo, Kang-Tae;Park, Sung-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.11
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    • pp.1627-1633
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    • 2010
  • The purpose of this study is to determine the possibility of Codonopsis lanceolata skin as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of C. lanceolata skin were measured. On a dry weight basis the contents of carbohydrate, crude protein, crude lipid and ash are 24.74, 2.73, 2.96 and 4.84%, and the calories of skin was 266.00 kcal/100 g and total dietary fiber was 64.73%. The contents of essential and non-essential amino acids were 633.40 and 870.72 mg/100 g wet weight basis. The K was the largest mineral followed by Ca, Mg, and P, suggesting that C. lanceolata skin is alkali material. The EDA of water extract from C. lanceolata skin was 18.28~79.30%, and the activity was dependent on the sample concentration. Total phenolic and flavonoids contents of water extract from C. lanceolata skin were estimated as 24.65 and $6.19\;{\mu}g/g$. The C. lanceolata skin extract showed the highest reducing power (3.5) at the concentration of 25 mg/mL. Based on the above results, we deemed that the C. lanceolata skin might have potential antioxidant activities. The general nutrients and antioxidant bioactive materials in C. lanceolata skin were also potential materials for good health food.

Composition Analysis of Various Blueberries Produced in Korea and Manufacture of Blueberry Jam by Response Surface Methodology (국내산 블루베리의 품종별 성분분석 및 반응표면분석법을 이용한 잼 제조)

  • Cho, Won-Jun;Song, Beom-Seok;Lee, Ju-Yeoun;Kim, Jong-Kon;Kim, Jae-Hun;Yoon, Yo-Han;Choi, Jong-Il;Kim, Gang-Sung;Lee, Ju-Woon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.319-323
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    • 2010
  • This study was carried out to investigate proximate compositions, acidity, and soluble solids of various blueberries produced in Korea and to prepare jam with optimized overall palatability by a response surface methodology. Proximate compositions were 75~88% in moisture, 0.32~0.62% in crude protein, 0.12~0.39% in crude lipid, and 10.18~23.80% in carbohydrate. Acidity and soluble solids of blueberries showed 0.82~1.58% and $7~12^{\circ}Brix$, respectively. The effect of sucrose ($X_1$, 200~300 g), pectin ($X_2$, 0~10 g), and citric acid ($X_3$, 0~0.5 g) on overall palatability of blueberry jam were investigated at five levels using a central composite design. Overall palatability of blueberry jam showed maximum score in 200 g blueberry, 248 g sucrose, 4.8 g pectin, and 0.26 g citric acid.

Crown Shape Control of Pinus koraiensis S. et Z. (VII) - The Influence of Thinning and Stem Pruning on Seed Component(The First Report) - (잣나무의 수형조절(VII) - 잣나무의 간벌과 수형조절이 종자의 성분에 미치는 영향(제1보) -)

  • Song, Jae-Mo;Shim, Tae-Heum;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
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    • v.18 no.1
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    • pp.87-96
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    • 2001
  • In order to investigate the influence of increased seed production of Korean white pine(Pinus koraiensis S. et Z.), component of seeds, produced from trees in the thinned stand and the stem-pruned trees in the seed orchard, were analyzed. The results obtained were as follows: 1. General component analysis of seed There is no significant influence of thinning on the seed component in moisture content (thinned- 4.10%, unthinned- 3.74% ), ash (thinned- 2.95%, unthinned- 2.94%), crude lipid (thinned- 67.62%, unthinned- 71.94%), and crude protein (thinned- 17.27%, unthinned- 17.50%). There is no significant influence of stem-pruning on the seed component in moisture content (unpruned- 4.26%, 1m stem-pruned- 4.10%, and 2m stem-pruned- 3.99%), ash (unpruned- 2.08%, 1m stem-pruned- 2.09%, and 2m stem-pruned- 2.15%) crude lipid(unpruned- 68.59%, 1m stem-pruned- 69.52%, and 2m stem-pruned- 72.53%), and crude protein (unpruned- 18.13%, 1m stem-pruned- 17.96%, and 2m stem-pruned- 17.56%) 2. Fatty acid analysis Seeds of tree from thinned and unthinned stands contained two essential fatty acids, i.e., linoleic acid (18:2) (thinned- 54.92%, unthinned- 55.40%) and linolenic acid (18:3) (thinned- 0.19%, and unthinned- 0.23%). Over 94% of fatty acids consisted of linoleic acid (18:2)(55%), oleic acid (18:1) (32%), and palmitic acid (16:0)(7%). T-test showed difference of content at 1% significance level for palmitoleic acid (16:1), stearic acid (18:0) and 8.11, 14-eicosatrienoic acid (20:3) and at 5% for linolenic acid (18:3), but no significant difference for three major fatty acids mentioned above, between two types of trees. Thus thinning may give no influence in fatty acid content. Seeds of three types of trees contained two essential fatty acids, i.e., linoleic acid (18:2)(unpruned- 55.25%, 1m stem-pruned- 54.74%, and 2m stem-pruned- 55.00%), and linolenic acid (18:3)(0.21% for three kinds of trees). Linoleic acid (18:2)(55%), oleic acid (18:1) (32%), and palmitic acid (16:0)(6%) consisted of more than 93% of all fatty acid content. At 5% significance level in F-test, difference was observed in the content of palmitoleic acid (16:1) and 8.11, 14-eicosatrienoic acid (20:3), but not in three major types of fatty acids above mentioned. It is observed that stem-pruning gave no significant influence in fatty acid content in general.

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Noisy Time Varying Vibration Signal Analysis using Adaptive Predictor-Binary Tree Structured Filter Bank System (적응예측기-이진트리구조 필터뱅크 시스템을 이용한 잡음이 부가된 시변 진동신호 분석)

  • Bae, Hyeon-Deok
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.10 no.1
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    • pp.77-84
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    • 2017
  • Generally, a time-varying vibration signal is generated in a rotating machine system, and when there is a failure in the rotating machine, the signal contains noise. In this paper, we propose a system consisting of an adaptive predictor and a binary tree filter bank for analyzing time - varying vibration signals with noise. And the vibration signal analyzed results in this system is used for fault diagnosis of the rotating machine. The adaptive predictor of the proposed system predicts the periodic signal components, and the filter bank system decomposes the difference signal between the input signal and the predicted periodic signal into subband. Since each subband signal includes a noise signal component due to a failure, it is possible to diagnose the failure of the using rotary machine. The validity of the proposed vibration signal analysis method is shown in the simulations, where the periodic components cancelled vibrating signals are decomposed to 32 subband, and the signal characteristics related faults are analyzed.

Analysis of Chemical Composition, Vitamin, Mineral and Antioxidative Effect of the Lotus Leaf (연잎의 일반성분, 비타민, 무기질 함량 분석 및 항산화 효과)

  • Lee, Kyung-Seok;Kwon, Yong-Jun;Lee, Ki-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.12
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    • pp.1622-1626
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    • 2008
  • This study was carried out for the analysis of chemical composition and antioxidative activity of lotus leaf. The lotus leaf contained 63.8% of crude carbohydrate, 16.9% of crude protein, 1.0% crude fat, and 9.3% of crude ash including high amount of calcium (2.2%). The antioxidative effect of several solvents extracts of lotus leaf was investigated. Among them 70% ethanol extract showed relatively higher extraction yield and higher total phenol content as well as the highest electron-donating ability using 1,1-diphenyl-2-picryl hydroxyl.

Compositions & Color of Codonopsis lanceolata Affected by Cultivation Methods (재배방법에 따른 더덕의 성분 및 색도)

  • Chung, Mi-Sook
    • Journal of the Korean Society of Food Culture
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    • v.14 no.5
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    • pp.529-534
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    • 1999
  • Amino acids, minerals and color of Codonopsis lanceolota that is one of Korean indigenous culinary herbs were analyzed. Arginine, glutamine, alanine and proline were the major total amino acids in wild, organic cultivated and cultivated Codonopsis lanceolata. The content of arginine was the highest free amino acid in wild, organic cultivated and cultivated Codonopsis lanceolata. The contents of arginine and K were the highest in organic cultivated Codonopsis lanceolata that was obtained by rice straw and fallen leaves application. Wild Codonopsis lanceolata was containing $3{\sim}5$ times Ca than organic cultivated and cultivated one. The contents of Pb and Cd were lower than 'countermeasure values for foodstuffs contamination'. Values of a, b and ${\Delta}E$ were significantly high in wild Codonopsis lanceolata than cultivated one.

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Technical Development of Hot Sauce with Red Pepper (생홍고추를 이용한 핫소스 제조 기술)

  • Kwon, Dong-Jin;Kim, Yoo-Jin;Lee, Sung;Yoo, Jin-Yong
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.391-396
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    • 1998
  • To inhibit the import of hot sauce, the manufacturing process of the Korean type hot sauce was developed. Chemical analysis of the imported hot sauce showed as follows; moisture 95.66% (w/w), salt 2.79% (w/w), titratable acidity 3.62% (w/w), crude fiber 0.42% (w/w), reducing sugar 0.03% (w/w), capsanthin 0.89% (w/w), capsaicin 4.28 mg% and pH 3.26. Korean type hot sauce prepared with red pepper, salt and vinegar was aged in oak barrel for 12 months at $10^{\circ}C$. Chemical analysis of the Korean type hot sauce showed as follows; moisture 92.05% (w/w), salt 8.82% (w/w), titratable acidity 3.88% (w/w), crude fiber 3.14% (w/w), reducing sugar 1.78% (w/w), capsanthin 0.13% (w/w), capsaicin 2.32 mg% and pH 3.29. Among chemical components, the contents of reducing sugar, salt and crude fiber except capsaicin and capsanthin were more than those of imported one. Chemical components of the Korean type hot sauce were changed slightly during aging for 12 months. The cell counts of yeast were increased from $3.2{\times}10^2\;to\;5.2{\times}10^6$ till the second month and those of lactic acid bacteria were increased from $3.{\times}10^1\;to\;1.5{\times}10^7$ till the third month, those of yeast and lactic acid bacteria decreased from the eighth month showed $6.6{\times}10^4$ and not detectable on the twelfth month, respectively. As the results of sensory evaluation on the Korean type hot sauce were evaluated to be superior to the imported to be superior to the imported one.

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Changes of Physicochemical Compositions in Domestic Broiler Chickens of Different Marketing Standard (국내산 닭고기의 유통규격별 이화학적 조성의 변화)

  • 채현석;유영모;조수현;박범영;김진형;안종남;이종문;윤상기;최양일
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.337-343
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    • 2001
  • Changes of Physicochemical compositions in domestic broiler chickens of different marketing standard range from 5ho (Korean traditional weight unite for chicken) to 16ho were assessed. The moisture contents of chicken breast were 74.00 ∼ 76.15% and there were no significant trend with weight difference. The chicken breasts of the light weight range(5ho∼8ho) contained high fat contents of 2.35∼2.78%. The chicken breasts of the heavy weight range(12ho∼16ho) contained low fat contents of 0.47 ∼0.66%. The chicken breasts of the heavy weight range had more protein contents than those of the light weight range. The chicken breast of the light weight range showed low Warner Braztler shear force (WBS) values when compared to those taken from chicken of the heavy weight range. However. there was no significant differences in water ho1ding capacity (WHC ) and cooking loss(p>0.05). In meat color the L* values (lightness) were 61.83∼71.33 and there were no significant differences for samples in different weight range except 5ho and 13ho. The b* valuers (yellowness) were 1.73 ∼6.79 and the values decreased with increasing chicken weight. In mineral composition. calcium and iron contents were decreased with increasing chicken weight. Phosphate, potassium and magnesium contents increased as weight ranges increased. The thigh meat separated from 11ho to 16ho had a similar tendency. In chemical composition(%). WBS, WBC. cooking loss, meat colors and mineral contents, compacted to breast in the same weight ranges. The results from this study would provide a basic information for establishment of marketing standard of chicken.

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