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Current occurrence of persimmon viroid and citrus viroid in persimmon in JellaNam-do and testing for viroid inactivation methods (전라남도 지역 감 바이로이드의 감염상황 및 무병화 효율 연구)

  • Kim, Dae Hyun;Kim, In-Soo;Lee, Gunsup;Cho, In-Sook;Cho, Kang Hee;Shin, Il Sheob;Kim, Se Hee;Chun, Jae An;Choi, In-Myung
    • Journal of Plant Biotechnology
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    • v.42 no.1
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    • pp.43-48
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    • 2015
  • It is a serious situation that the farmers' income has gradually decreased due to the decline of productivity of fruit trees infected with viroids. It has been known that Persimmon viroid (PVd) and Citrus viroid (CVd) are economically important viroids that can infected persimmon. In this study, the incidence of CVd and PVd in 'Fuyu' persimmon were identified as 41% and 34% in JeollaNam-do, respectively. The collected persimmon samples infected by both PVd and CVd were used for testing efficiency of the viroid inactivation methods. The samples were subjected to single treatment of the heat treatment ($37^{\circ}C$), cold treatment ($4^{\circ}C$), or antiviral agent treatment (Ribavirin), and double treatment of combinations of the three methods. Viroid inactivation efficiency was confirmed through RT-PCR. In the case of the samples subjected to cold treatment for 4 weeks, the viroid inactivation efficiency was most significantly high as 67% against the survival rate of 100%. In addition, in the case of the samples treated for 2 weeks with the antiviral agents and cold treatment, the viroid inactivation rate was similar to that of the cold treatment. In conclusion, the cold treatment showed the highest viroid inactivation efficiency, and this result will provide valuable information for production of viroid-free persimmon.

Clinical Outcomes and Prognosis of Patients with Stent Fracture after Successful Drug-Eluting Stent Implantation (관상동맥 약물 방출 스텐트 삽입 후 스텐트 골절에 대한 임상결과 및 예후)

  • Kim, In-Soo;Han, Jae-Bok;Jang, Seong-Joo
    • Journal of radiological science and technology
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    • v.37 no.2
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    • pp.109-116
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    • 2014
  • Many studies have suggested that in the era of Drug-Eluting Stents(DES) are one of the causes of In-Stent Restenosis(ISR) of Stent Fracture(SF). The present study sought to evaluate clinical characteristics of patients with stent fracture after successful DES implantation. The 4,701 patients were selected for analysis who underwent a follow-up coronary angiography irrespective of ischemic symptoms. The overall incidence of SF was 32 patients(male:female=19:13, Av. age $62.44{\pm}9.8$year, 0.68%). Fractures of Sirolimus-Eluting Stents(SES), Paclitaxel-Eluting Stents(PES), Biolimus A9-Eluting Stents(BES), Everolimus-Eluting Etents(EES), Endothelial Progenitor Cell Capture Stent(EPC) and Zotarolimus-Eluting Stents(ZES) are accounted for 19(59.4%), 9(28.1%), 2(6.3%), 1(3.1%), 1(3.1%) and 0(0%) respectively. SF developed in the left Anterior Dscending(LAD) artery in 16 patients(50%) and in complex(type B2, C) lesions in 25 patients(69.4%). Ten patients were treated with heterogenous DES, the rest being treated with either homogenous DES(3 patients), plain old balloon angioplasty(3 patients), or conservative medical treatment(17 patients). None of the patients with SF suffered from cardiac death during a follow-up period of $32.9{\pm}12.4$ months. The overall rate of DES fracture over up to 3.7 years of follow-up was 0.68% with higher incidence in SES than in PES. SF frequently occurred in the LAD artery and in complex lesions. Of the patients with SF, coronary intervention was performed only when the binary restenosis lesion was significant. During the follow-up, patients with SF have continued on combination antiplatelet therapy. There is a very low rate of major adverse cardiac events(post-detection of SF), especially cardiac death associated with SF.

A Study for Quality of Life in Musically Talented Students Using Experience Sampling Method (경험표집법(ESM)을 통해 본 음악영재의 삶의 질)

  • Lee, Hyun-Joo;Choe, In-Soo
    • Journal of Gifted/Talented Education
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    • v.21 no.1
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    • pp.57-81
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    • 2011
  • The purpose of this study was to explore the quality of life of musically talented students as measured by their external experiences (e.g., activities, companions) and internal experiences (e.g., flow, emotion). The participants in this study were 33 musically talented students (10 males, 23 females) aged 13 to 19. Study data were collected for 7 consecutive days using the Experience Sampling Method (ESM), which employs a cellular-phone as a signaling device. The results were as follows: First, in response to the 1625 random signals, musically talented students reported that 40.9% of their time was spent on productive activities. An additional 33.4% of time was used for maintenance activities and the rest of their time was spent on leisure/social activities. Also, musically talented students reported that 48.5% of their time was spent alone. When they were alone, they spent a lot of time engaging in productive activities (44.3%). Second, in order to measure the flow of their life, two methods were used. One used a 4-channel flow model (i.e. apathy, boredom, flow, anxiety) and the other used 8 dimensions and conditions of the flow experience (i.e. concentration, self-consciousness disappears, action and awareness merge, distorted sense of time, freedom from worry about failure, clear goals, immediate feedback, balance between challenges and skills). According to the former, when engaged in music-related activities, musically talented students usually reported flow (54.0%), while they felt apathy (41.3%) for daily routines activities. According to the latter method, musically talented students experienced flow for most productive activities, while they experienced flow least for maintenance activities. Emotional variables of ESF are comprised of 10 semantic scales (i.e. happy-sad, strong-weak, active-passive, sociablelonely, proud-ashamed, involved-detached, excited-bored, clear-confused, relaxed-worried, cooperative-competitive). Musically talented students reported experiencing the most positive emotion for social activities and experiencing the most negative emotion for maintenance activities. Results of this study assert that musically talented students had to trade off immediate enjoyment for developing their special gifts. They could not afford as much time for socializing with friends, and they had to spend more time alone compared to their peers without such gifts. Consequently, they were found to deprive themselves of the spontaneous good times that teenagers usually thrive on. They were helped in this respect by their autotelic personality traits, especially their strong need for achievement and endurance. The downside, however, is that the moment-to-moment quality of their moods suffered. The argument concerning musically talented students applies for all adolescents. The choices that talented students must make between immediate gratification and long-term development, and between solitude and companionship, are the same choices every young person must make, regardless of her or his level of talent. All of us have gifts that are potentially useful and worthy of being appreciated. But to develop these latent talents we must cultivate them, and this takes time and the investment of mental energy. The lifestyle that musically talented students develop can show us some of the choices all of us must make in order to cultivate our gifts.

Quality Stability of Powdered Soup Using Powder from Oyster Wash Water (굴 세척액 유래 분말수프의 품질안정성)

  • Heu, Min-Soo;Lee, Jung-Suck;Kim, Poong-Ho;Cho, Moon-Lae;Ahn, Hwa-Jin;Shim, Hyo-Do;Kim, Jin-Soo;Kim, In-Soo
    • Applied Biological Chemistry
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    • v.44 no.4
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    • pp.224-229
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    • 2001
  • This study was carried out to examine the quality stability of powdered soup using powder from oyster wash water (PSW). To compare the quality stability, powdered soup from oyster hot-water extracts (PSE) was also prepared by mixing hot-water extract powder (15 g), table salt (5 g), cream powder (19 g), milk replacer (12 g), wheat flour (20 g), corn flour (15 g), starch (5 g), glucose (7.5 g), and onion powder (1.5 g). In preparing PSW, powder from oyster wash water, instead of powder from oyster hot-water extracts, was added and other additives were the same proportion as PSE. The PSW and PSE were packed with laminated film bag (OPP,$20\;{\mu}m$; PE, $20{\mu}m$; paper, $45\;g/m^3$; PE, $20\;{\mu}m$; Al, $7\;{\mu}m$; PE, $20\;{\mu}m$), and then stored at ambient temperature for 12 months. The moisture content, water activity, peroxide value, and fatty acid composition showed little changes during storage of the PSW, The pH, volatile basic nitrogen content, and brown pigment formation increased slightly, while white index decreased slightly during storage of PSW. No significant difference was observed in the changes of food components between PSW and PSE during storage. According to a sensory evaluation, the change in quality of PSW was negligible during 12 months of storage. From the results of the chemical experiment and sensory evaluation, PSW packed with laminated film bag (OPP, $20\;{\mu}m$; PE, $20\;{\mu}m$; paper, $45\;g/{\mu}m$; PE, $20\;{\mu}m$) was revealed to be preserved in good quality during 12 months of storage.

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Prevalence and Clinical Characteristics of Pertussis in Children, Cheonan, Korea (천안지역 소아 백일해 감염의 유병률과 임상적 고찰)

  • Lee, Kun Song;Son, Jae Sung;Chung, Eun Hee;Bae, Hong Ki;Lee, Mee Jeong;Yu, Jeesuk;Chang, Young Pyo;Park, Woo Sung;Kim, Jae Kyoung;Rheem, Insoo;Roh, Eui-Jung
    • Pediatric Infection and Vaccine
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    • v.16 no.2
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    • pp.175-182
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    • 2009
  • Purpose : A number of countries have experienced an increase in pertussis during the past decade. In particular, there has been an increase in the incidence rate among adolescents and adults. To learn more about the current epidemiology of pertussis, we studied the prevalence and clinical characteristics of pertussis in children in Cheonan, South Korea. Methods : We collected nasopharyngeal aspirates of 118 patients who were treated for respiratory symptoms at Dankook Univeristy Hospital between March 2008 and September 2009. We performed multiplex PCR for detection of Bordetella pertussis in those aspirates. Results : Of the 118 patients, 10 (8%) were positive by PCR for B. pertussis. Six episodes occurred during the period July to September 2009. Nine of the 10 patients were less than 3 months old. Seven of them had not received DTaP vaccine. The mean duration of coughing before diagnosis was 10.9${\pm}$5.2 days. Ten patients (100%) had paroxysmal cough and 8 (80%) had post-tussive vomiting. Only one patient had fever. One who had complications that include pneumonia, atelectasis and pneumomediastinum developed an absolute increase in leukocyte count (84,400/$mm^3$). There was a statistically significant relation between vaccine being received and development of complications (P =0.033). Conclusion : We suspect that there was an epidemic of pertussis between July and September 2009. Further investigation by a pediatric or nationwide surveillance system is needed to monitor the changing epidemiology for pertussis.

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Performance of Growing Period of Two-way Crossbreed Parent Stock for Producing of Laying-Type Korean Native Commercial Chickens (산란전용 토종 실용계를 생산하기 위한 2원교배 종계의 육성 능력 검정)

  • Hong, Eui-Chul;Choo, Hyo-Jun;Kim, Hak-Kyu;Kim, Chong-Dae;Heo, Kang-Nyeong;Lee, Myeong-Ji;Son, Bo-Ram;Suh, Ok-Suk;Choi, Hee-Cheol;Kang, Bo-Seok
    • Korean Journal of Poultry Science
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    • v.39 no.3
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    • pp.177-182
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    • 2012
  • This work was carried out to investigate the performance of growing period of two-way crossed of Korean native chickens parental stocks. A total of four hundred eighty female 2-crossbred chicks were used in this study and they were from National Institute of Animal Science. Groups were four crossbreds (4 replications/crossbred, 30 birds/replication) as A) C strain ${\times}$ Y strain, B) C strain ${\times}$ L strain, C) C strain ${\times}$ G strain and D) C strain ${\times}$ W strain. Body weight of A crossbred was the highest at the age of 8 week (P<0.05) and that of D strain was the lowest for growing period (P<0.05). Body weights of A and B crossbreds were higher than those of C and D crossbreds at the 12 and 16 weeks (P<0.05). Weekly body weights of A and B crossbreds were higher than C and D crossbreds (P<0.05), and weekly body weight of B crossbred was higher compared to other crossbreds at 0~20 weeks old. Weekly feed intake of D crossbred was the lowest among all crossbreds at 0~12, 0~16 and 0~20 weeks old (P<0.05). Weekly feed conversion ratio of C crossbred was the highest among crossbreds (P<0.05). These results can give the basic information for growth related data in 2-way crossbreed Korean Native Chickens, which can be used for the parental stocks for the laying-type of Korean native commercial chickens.

Study on the Combination of In-situ Chemical Oxidation Method by using Hydrogen Peroxide with the Air-sparging Method for Diesel Contaminated Soil and Groundwater (과산화수소를 이용한 현장원위치 화학적 산화법과 공기분사법(Air-sparging)을 연계한 디젤 오염 토양/지하수 동시 정화 실내 실험 연구)

  • Kim, Nam-Ho;Kim, In-Su;Choi, Ae-Jung;Lee, Min-Hee
    • Journal of Soil and Groundwater Environment
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    • v.11 no.6
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    • pp.8-17
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    • 2006
  • Laboratory scale experiments were performed to investigate the removal efficiency of the in-situ chemical oxidation method and the air-sparging method for diesel contaminated soil and groundwater. Two kinds of diesel contaminated soils (TPH concentration : 2,401 mg/kg and 9,551 mg/kg) and groundwater sampled at Busan railroad station were used for the experiments. For batch experiments of chemical oxidation by using 50% hydrogen peroxide solution, TPH concentration of soil decreased to 18% and 15% of initial TPH concentration. For continuous column experiments, more than 70% of initial TPH in soil was removed by using soil flushing with 20% hydrogen peroxide solution, suggesting that most of diesel in soil reacted with hydrogen peroxide and degraded into $CO_2$ or $H_2O$ gases. Batch experiment for the air-sparging method with artificially contaminated groundwater (TPH concentration : 810 mg/L) was performed to evaluate the removal efficiency of the air-sparging method and TPH concentration of groundwater decreased to lower than 5 mg/L (waste water discharge tolerance limit) within 72 hours of air-sparging. For box experiment with diesel contaminated real soil and groundwater, the removal efficiency of air-sparging was very low because of the residual diesel phase existed in soil medium, suggesting that the air-sparging method should be applied to remediate groundwater after the free phase of diesel in soil medium was removed. For the last time, the in-situ box experiment for a unit process mixed the chemical oxidation process with the air-sparging process was performed to remove diesel from soil and groundwater at a time. Soil flushing with 20% hydrogen peroxide solution was applied to diesel contaminated soils in box, and subsequently contaminated groundwater was purified by the air-sparging method. With 23 L of 20% hydrogen peroxide solution and 2,160 L of air-sparging, TPH concentration of soil decreased from 9,551 mg/kg to 390 mg/kg and TPH concentration of groundwater reduced to lower than 5 mg/L. Results suggested that the combination process of the in-situ hydrogen peroxide flushing and the air-sparging has a great possibility to simultaneously remediate fuel contaminated soil and groundwater.

A study on determination of the lime requirement based on exchangeable aluminum content (치환성(置換性) Al 함량(含量)에 따른 석탄소요량(石炭所要量) 결정(決定)에 관(關)한 연구(硏究))

  • Ryu, In Soo;Cho, Seong Jin;Yuk, Chang Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.3
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    • pp.185-191
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    • 1974
  • Incubation and pot studies were conducted with upland soils for a study on determination of the lime requirement based on exchangeable alumium content. The results obtained are as follows; 1. Results of chemical analysis of upland soils show that pH varies from 5.0 to 5.4, and exchangeable Al moves with the range of 1.3-3.0m.e/100gr. Exchangeable Al decreases with years of cultivation. 2. Incubation studies shows that on acid mineral soils almost all exchangeable Al, on average 95% was neutralized with the lime to neutralized 100% exchangeable Al. On volcanic ash soil, however, only 65.5% was neutralized with the lime estimated to neutralize the equivalent of 200% exchangeable Al. The latter has required more lime. 3. The pH of mineral soils is on the average increased from an initial 5.2 to 6.3 when 95% of exchangeable Al is neutralized, whereas that on volcanic ash soil is increased from an initial 5.3 to 5.5 only when lime is applied at rate to neutralize the equivalent of 200% exchangeable Al. 4. A high correlation coefficient (r=0.99) was obtained between exchangeable Al and exchangeable acidity. This indicates that exchangeable acidity is primarly a result of exchangeable Al. 5. In pot experiments with soybean cultivated on one of the hill land soils (Songjoong soil) the application of fused phosphate and triple superphosphate based on a 5% saturation rate ($P_2O_5$ 32.1 kg/10a) showed that the liming factor for calculation of the optimum lime requirements based on exchangeable acidity was 0.594 for fuses phosphate or 1.132 for tripple superphosphate, and optimum pH is approximately 6.0 and optimum neutralization rate of exchangeable Al is 80-90%.

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Determination of the Optimum Rates of P and K Fertilizer Application for Tong-il Line Rices in Different Paddy Soils (통일계(統一系) 수도품종(水稻品種)에 대(対)한 답토양별(畓土壤別) 인산(燐酸) 및 가리시비적량(加里施肥適量))

  • Lee, Choon-Soo;Huh, Beom-Lyang;Ryu, In-Soo;Park, Chon-Suh;Ko, Mi-Suk
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.2
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    • pp.101-109
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    • 1982
  • An attempt to determine the optimum levels of P and K fertilizers application for Tong-il lines (indica${\times}$Japonica) was made with the data obtained from the farm fields during 1976 to 1979. The detailed interpretation to obtains relationships between fertilizer recommendation of P and K with their balance with Ca and Mg contents in soil were made using yield data obtained in 1977. The results were summarized as follows : 1. The optimum rates of P and K fertilizer application varied with the kinds of paddy soils showing the ranges of 6.6-11.4 kg/10a for P (as $P_2O_5$) and 7.0-11.3 kg/10a for K (as $K_2O$). The amounts of optimum fertilizers increased in the order of unmatured soil, normal soil, sandy soil, saline soil, poorly drained soil for P, and unmatured soil, poorly drained soil, sandy soil, normal soil, saline soil for K. 2. The yield increment at the optimum levels of P and K in comparison with no fertilizer application were 3,5-7.5% for P and 2.1-9.1% for K. The effectiveness of P was greatest in the unmatured soils and that of K was greatest in the poorly drained soils, and in the saline soil, that of P and K was relatively high. 3. According to relationship between relative yield index and soil testing value, the critical $P_2O_5$ contents which showed the yield response in soil were about 100 ppm for normal soil and 200ppm for sandy soil. That of exchangeable K/Ka+Mg ratio in soil were about 0.08 for normal paddy soil and over 0.08 for sandy soil, and those for poorly drained soils were not obtained in the ranged below 0.08. 4. The regression equations of fertilizer recommendation for different soils were obtained between the available $P_2O_5$ in soil or ratio of K to base including Ca and Mg in soil (x) and the amount (Y) of P and K fertilizers applied. The equations for phosphorus recommendation were Y=11.27C-0.048x for normal paddy soil and Y=13.383-0.061x for sandy soil, and those for potassium recommendation were Y=9.526-0.569x for normal paddy soil, Y=11.727-1.004x for sandy soil, and Y=12.574-0.558x for poorly drained soil, respectively.

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Postpartum Reproductive Tract Recovery and Prevalence of Health Problems in Dairy Cows (젖소의 분만 후 생식기의 회복 및 질병 발생 조사)

  • Jeong, Jae-Kwan;Choi, In-Soo;Kang, Hyun-Gu;Jung, Young-Hun;Hur, Tai-Young;Kim, Ill-Hwa
    • Journal of Veterinary Clinics
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    • v.32 no.2
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    • pp.168-173
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    • 2015
  • This study estimated the degree of uterine inflammation and resumption of ovarian cyclicity in postpartum dairy cows. In addition, the prevalence of health problems during calving and the postpartum period was investigated. A total of 224 Holstein dairy cows from four dairy farms in Chungcheong Province were used for the study. Uterine discharge was scored on a 0 to 5 rank scale (metricheck score) using a metricheck instrument from 1 to 8 weeks postpartum and uterine cytology (neutrophil level) was examined at 4, 6 and 8 weeks postpartum to evaluate uterine inflammation. Resumption of ovarian cyclicity was evaluated based on progesterone analysis (${\geq}1ng/mL$) at 4, 6 and 8 weeks postpartum. Postpartum disorders were diagnosed by veterinarians in the research team. The metricheck score decreased linearly (p < 0.0001) from weeks 1 ($3.4{\pm}0.1$) to 4 ($2.0{\pm}0.1$) postpartum, and then remained at that level through week 8 postpartum ($1.6{\pm}0.1$, p > 0.05). The neutrophil levels determined by uterine cytology assays also decreased (p < 0.0001) from week 4 ($22.3{\pm}1.8%$) to 6 ($12.1{\pm}1.4%$) postpartum, and then remained at that level through week 8 postpartum ($9.2{\pm}1.4%$, p > 0.05). The proportion of cows exhibiting the ovarian cyclicity increased linearly (p < 0.0001) from weeks 2 (12.1%) to 8 (74.3%) postpartum. The prevalence of dystocia and retained placenta were 20.5% and 30.4%, respectively. The prevalence of ketosis, milk fever, abomasal displacement, septicemic metritis, endometritis, subclinical endometritis, pyometra, and ovarian cysts were 18.8%, 3.6%, 4.0%, 36.2%, 29.5%, 17.0%, 4.5%, and 14.7%, respectively. The prevalence of digestive problems and mastitis were 5.4% and 9.6%, respectively. The prevalence of culling and death were 4.0% and 3.6%, respectively. The prevalence of dystocia, retained placenta, septicemic metritis, endometritis, ovarian cysts, digestive problems, mastitis, and culling differed among farms (p < 0.05 to 0.01). In conclusion, proper examinations for uterine inflammation and ovarian cyclicity during 4 to 8 weeks postpartum are useful clinical tools to maintain herd reproductive health. Dystocia, retained placenta, septicemic metritis, and endometritis were predominant disorders in dairy cows of the survey area.