• Title/Summary/Keyword: 인공수정체

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Intraspecies Androgenesis in Mud Loach (Misgurnus mizolepis): I. Optimization of the Egg Inactivation and Haploid Androgenesis Using Transgene Marker (미꾸라지(Misgurnus mizolepis)의 웅성발생성 처녀생식: I. 형질전환 유전자 표지를 이용한 웅성발생성 반수체 유도의 최적화)

  • Nam Yoon-Kwon;Noh Choong-Hwan;Kim Dong-Soo
    • Journal of Aquaculture
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    • v.19 no.3
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    • pp.166-172
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    • 2006
  • Induced androgenesis, a form of artificial parthenogenesis is an important tool for the generation and use of genetically isogenic or clonal fish strain. An optimized protocol for the genetic inactivation of mud loach (Misgurnus mizolepis) oocytes (i.e. production of androgenetic haploid) was developed using UV-irradiation. Various dose levels of UV significantly affected the fertilizing capacity of the eggs, hatchability of embryos and incidence of haploidy. Based on the extensive examinations of treatment conditions on embryo viability and haploid incidence, the optimum dose level of UV irradiation was turned out to be $10,800ergs/mm^2$ with 56.9% of hatching success and 94.6% of haploidy. The overall yield of putative androgen under optimized treatment condition was more than 50% out of total eggs inseminated. The success of androgenetic reproduction of haploid genome was verified by flow cytometry and PCR amplification of transgene that is exclusive to either one of parental sexes. However, a small portion $(8\sim11%)$ of presumed androgenetic haploid larvae was proven to contain residual DNA fragment(s) from maternal parent.

Study on sensitivities of generalized RRI method for data analysis of CSAMT survey (인공전류원 MT탐사 자료해석을 위한 GRRI법의 감도해석에 관한 연구)

  • Kim, Hee-Joon;Park, Mi-Kyung;Seol, Soon-Jee
    • 한국지구물리탐사학회:학술대회논문집
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    • 2005.05a
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    • pp.281-286
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    • 2005
  • This paper presents sensitivity analysis of generalized rapid relaxation inversion (GRRI) algorithm for inverting controlled-source audio-frequency magnetotelluric (CSAMT) data. The algorithm was originally developed by modifying the RRI algorithm to recover a two-dimensional (2-D) conductivity structure of the Earth from MT data, but can be extended to include CSAMT data if it is combined with 2.5-D forward modeling. These GRRI approximate sensitivities are validated by comparison with exact 1-D and 2.5-D sensitivities. The comparison shows that the GRRI sensitivity is a good approximation to the exact sensitivity and has about half magnitude of the RRI sensitivity. Although the magnitude of the GRRI sensitivity is still slightly larger than that of the 2.5-D sensitivity, both sensitivities are broadly similar in shape when source-receiver offsets are greater than one skin depth on the Earth.

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Studies on the Fertilization of Pulsatilla koreana (할미꽃(Pulsatilla koreana)의 수정현상(受精現象)에 관(關)한 연구(硏究))

  • Lee, Man-Sang
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.1
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    • pp.81-85
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    • 1994
  • This experiment was carried out to investigate the fertilization and the size of mature female and male gametophytic parts of Pulsatilla koreana after artificial pollination. The size of pollen is $26.5{\mu}m$ at the time of anther dehiscence, that is, about $3{\sim}4$ days after pollination. Synergid nucleus, egg nucleus, and polar nucleus are 10.0, 15.0, and $32.5{\mu}m$ respectively at the time of completing egg apparatus formation, that is, about 2 days after pollination. Poller tubes germinate on stigma about 10 hours, passing lower part of style about 30 hours, penetrating into micropyle about 35 hours after pollination. Sperm nucleus penetrates into polar nucleus about 40 hours and egg cell about 48 hours after pollination. But, there seems to be different among the individuals. Multinuclei and multinucleoli are formed in egg cell, synergid, and polar nucleus about the time of fertilization. Proembryo is formed about 4 days, being changed to large globular form about $6{\sim}8$ days after pollination. Endosperm nuclei divide into free nuclei after fertilization and change to cotyledon in gymnosperm. There seems to be same phenomena in Pulsatilla koreana.

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The Study on Accommodation for eyes with pseudo-crystalline lenses (인공수정체안의 조절반응에 관한 연구)

  • Lee, Seok-Ju;Sung, A-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.203-209
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    • 2004
  • Physiological accommodation in aphakia has been reported 0.50D. Also, accommodation appears in pseudo-crystalline lens. However accommodation was not clear how to works in the eyes. Therefore, the purpose of this study is that accommodation has been affected to changes of axial length and corneal refractive power when the accommodative stimulus was taken 10 diopters to person who has pseudo-crystalline lens. To determine of the accommodative response of pseudo-phakic patients who had no side effects over 2 months, we have measured the axial length, corneal refractive power in 48 patients(96 eyes). When the accommodative stimulus was taken about 10 diopters, the axial length increased by $0.05{\pm}0.06mm$ and corneal refractive power increased $0.04{\pm}0.24$ diopter. The changes of axial length in the myopia was increased by $0.19{\pm}0.18D$, emmetropia was increased by $0.12{\pm}0.16D$ and hyperopia was $0.10{\pm}0.19D$. The results of measurement revealed that the mean value was increased $0.03{\pm}0.13D$ in the myopia, and increased $0.21{\pm}0.13D$ in the emmetropia, and decreased $0.02{\pm}0.34D$ in the hyperopia.

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Conceptual Design of Multi-Functional Structure using Rectangular Grid-Stiffened Structure for Satellite (위성용 사각형 격자강화 구조의 다기능 구조체 개념설계)

  • Seo, Hyun-Suk;Jang, Tae-Seong;Rhee, Ju-Hun;Kim, Won-Seock;Hyun, Bum-Seok;Lim, Jae-Hyuk;Hwang, Do-Soon;Lee, Sang-Kon;Cho, Hee-Keun;Han, Eun-Soo;Kim, Im-Soo;Sim, Eun-Sup
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.39 no.6
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    • pp.526-534
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    • 2011
  • The MFS (Mlti-Functional Structure) concept, which integrates the electronics, thermal control and structure into a single packaging system, has been developed and applied to reduce the volume and weight of the satellite. Therefore, this MFS can eliminate the bulky chassis/frames, cables and connectors of the electronic equipment. The main point of this traditional MFS is the replacement of the electrical chassis/frames with MCMs (Multi-Chip Modules) that require much costs and efforts for developing. This paper shows the new MFS concept that effectively saves the volume and weight. The structure including the thermal control and radiation shielding elements will be designed and manufactured as the rectangular grid-stiffened structure. The rectangular grid-stiffened structure is the modification of the iso-grid structure, and provides the enough spaces for putting the general PCBs without the chassis/frames.

A ground condition prediction ahead of tunnel face utilizing time series analysis of shield TBM data in soil tunnel (토사터널의 쉴드 TBM 데이터 시계열 분석을 통한 막장 전방 예측 연구)

  • Jung, Jee-Hee;Kim, Byung-Kyu;Chung, Heeyoung;Kim, Hae-Mahn;Lee, In-Mo
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.21 no.2
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    • pp.227-242
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    • 2019
  • This paper presents a method to predict ground types ahead of a tunnel face utilizing operational data of the earth pressure-balanced (EPB) shield tunnel boring machine (TBM) when running through soil ground. The time series analysis model which was applicable to predict the mixed ground composed of soils and rocks was modified to be applicable to soil tunnels. Using the modified model, the feasibility on the choice of the soil conditioning materials dependent upon soil types was studied. To do this, a self-organizing map (SOM) clustering was performed. Firstly, it was confirmed that the ground types should be classified based on the percentage of 35% passing through the #200 sieve. Then, the possibility of predicting the ground types by employing the modified model, in which the TBM operational data were analyzed, was studied. The efficacy of the modified model is demonstrated by its 98% accuracy in predicting ground types ten rings ahead of the tunnel face. Especially, the average prediction accuracy was approximately 93% in areas where ground type variations occur.

CLINICAL STUDY OF MAXILLOFACIAL PROSTHESES;OSSEOINTEGRATED IMPLANTS FOR MAXILLOFACIAL PROSTHESES (악안면 보철의 임상적 고찰;골내 매식술을 통한 보철적 회복에 관하여)

  • Min, Seung-Ki
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.23 no.5
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    • pp.406-414
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    • 2001
  • In recent decade, there has been a very rapid development in technical possibilities to provide patients with maxillofacial prostheses. Dr. Brenemark first introduced possibility of use of maxillofacial interosseous implant in patients with ablative tumor surgery in 1979. He did introduce the new type of maxillofacial implants system which widen the fixture flange on top of the bone. The advantages of fixed prostheses with implants were well known to various ways, easy to attach, keep clean prostheses, and not to disturb recipient bed. But there are some problems to install implants on maxillofacial regions, because mostly facial bone has very thin cortical bone and poor bone quality. It needs more retention between implant and bone which overcome that fault with fixture flange. To make maxillofacial prostheses, it should be understood general procedure of maxillofacial laboratory work. Ear and midface maxillofacial fabrication which include nose and eye defects will be described step by step.

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Changes in Medical Practice Pattern before and after Covering Intraocular Lens in the Health Insurance (인공수정체 보험급여 전.후 진료양상의 변화)

  • Choi, No-Ah;Yu, Seung-Hum;Min, Hey-Young;Chung, Eun-Wook
    • Journal of Preventive Medicine and Public Health
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    • v.27 no.4 s.48
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    • pp.807-814
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    • 1994
  • This study is to find out changes in medical practice at a university hospital before and after covering intraocular lens (IOL) from the health insurance benefit. The coverage started on March 1, 1993 and a total of 596 cases who were discharged from July 1 to December 31, 1992 and 580 cases who were discharged from July 1 to December 31, 1993 were analyzed. Since the standard reimbursement scheme was changed from March 1, 1993, the charges for 1992 were transformed into 1993 scheme. Major findings are as follows: Average length of stay was statistically significantly decreased from 8.24 days in 1992 to 6.86 days in 1993. Charges except IOL has been statistically significantly decreased from 501,000 Won in 1992 to 444,000 Won in 1993. Charges for drugs and injection have been reduced. However, charge per day for them was not much different. This is due to decrease in length of stay. Charges for laboratory tests and radiologic examination were quite the same. Charges which are not covered by the insurance remained the same. The revenue of the hospital was reduced as expected. However, the hospital reduced the length of stay and increase the turnover rate In order to compensate the potential loss of revenue due to the difference of reimbursement between the out-of-pocket expense and the insurance coverage. By introducing the IOL benefit in the insurance, the insured pays less, hospital generates more revenue through shortening the hospital stay, and the total medical care cost becomes less nationwidely.

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Design of Small Optical Tracker for Use in the Proving Ground (시험장 환경에 적합한 소형 광학추적기 설계)

  • Park, Sanghyun
    • Journal of Advanced Navigation Technology
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    • v.24 no.3
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    • pp.224-231
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    • 2020
  • An optical tracking plays an important role for measurement operation, as it is responsible for low altitude measurements that are difficult to obtain with radar systems. Since the existing optical tracking systems have not been developed in the proving ground itself so far, it is difficult to modify them to fit the environment of the proving ground. Also, they are designed as a vehicle-mounted type, so there is a limitation in selecting an optimal site. The in-house developed small optical tracking system is designed with a simple configuration to overcome these shortcomings and makes it possible for operators to operate the system at any place in the proving ground. In addition, there has been a need of developing small optical trackers by ourselves to be prepared for future research so that artificial intelligence (AI) can be applied to the optical tracking systems. In this paper, we described the design concept of the small optical tracker, the configuration of the components to implement the basic tracking function, and showed the results of the simulation to set the configuration of the equipment according to the characteristics of the flight targets.

Seed Production by Induction of Male Flowers on Female Plants of Hemp(Cannabis saliva L.) (대마 자주의 웅화 유도에 의한 자성종자 생산에 관한 연구)

  • Moon, Youn-Ho;Koo, Bon-Cheol;Choi, Yoyng-Hwan;Ahn, Seung-Hyun;Bark, Surn-Teh;Cha, Young-Lok;An, Gi-Hong;Kim, Jung-Kon;Suh, Sae-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.4
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    • pp.327-332
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    • 2010
  • This study was conducted to overcome the deliciousness by sex reversal in hemp plants. When female plants grown under long day length condition were transferred to short day length condition in 3th and 6th leaf stage, a few male flowers were formed and the gap of flowering days between sex reversed male and normal female flowers were around 10 days and 3 days, respectively. Although the flowering amounts were small, this short gaps of flowering days were enough for artificial pollination. When female plants grown under long day length condition were transferred to short day length condition just after treatment of silver nitrate in the vegetative stage, a great amount of male flower was formed and the gap of flowering days between sex reversed male and normal female flowers were only 1 to 3 days. The increased sex reversed male flowers and induced short gap of flowering days were adequate for natural wind pollination. When female seeds were cultivated, seed yield was 141 kg/10a, while it was 96 kg/10a when normal seeds cultivated.