• Applied Biological Chemistry
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• v.48 no.1
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• pp.98-102
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• 2005

The MeOH extracts obtained from whole plant of Trigonotis peduncularis Benth. were solvent fractionated using EtOAc, n-BuOH and water, successively. The EtOAc and n-BuOH fractions gave four flavonol glycosides through application of silica gel and octadecyl silica gel (ODS) column chromatographies. The chemical structures of the flavonol glycosides were determined by the interpretation of several spectral data including 2D-NMR as $kaempferol-3-O-{\beta}-{D}-glucopyranoside\;(astragalin,\;1),\;kaempferol-3-O-{\alpha}-{L}-rhamnopyranosyl\;(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(nicotiflorin,\;2),\;quercetin-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(rutin,\;3),\;quercetin-3-O-{\beta}-{D}-glucopyranoside\;(isoquercitrin,\;4)$. The flavonoids have been first isolated from this plant. Nicotiflorin $(100\;{\mu}g/ml)$ showed $68.3{\pm}1.2%$ of the inhibitory effect on hACAT1(human Acyl CoA: cholesterol transferase 1) activity.

• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.197-203
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• 2009

An indole oxygenase originated from Rhodococcus sp. RHA1 was cloned into the expression vector, pTrc99A, in Escherichia coli, and designated pTCAN1. The pTCAN2 was constructed from pTCAN1 by the deletion of $lacI^q$ for the constitutive expression of indole oxygenase without adding IPTG in the medium. The complete open reading frame of indole oxygenase was 1,224 bp long, which encodes a protein of 407amino acids. Crude extracts of E. coli $DH5{\alpha}$/pTCAN1 and pTCAN2, respectively, were prepared and subjected to SDS-PAGE analysis. A band corresponding to molecular mass of about 43 kDa was appeared and this result correlated with the predicted molecular mass of cloned indole oxygenase. The E. coli harboring pTCAN1 and pTCAN2, respectively, showed blue color colony in LB plate. The pigment showing blue color was prepared from E. coli $DH5{\alpha}$/pTCAN2, and identified as indigo by experiments using spectrophotometer, HPLC, and TLC. The indigo-forming activity of indole oxygenases from the whole cell of E. coli $DH5{\alpha}/pTCAN1$ cultured at LB medium added 1mM of IPTG and that of E. coli/pTCAN2 showed about 1.75nmol/min/mg DCW (dry cell weight) and 3.85 nmol/min/mg DCW, respectively. Also, the E. coli $DH5{\alpha}$/pTCAN2 produced about $236{\mu}M$ of indigo after 48 hours incubation in TB medium supplemented with 2.5 mM of tryptophan.

• KIPS Transactions on Software and Data Engineering
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• v.5 no.4
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• pp.187-194
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• 2016

When performing experiments in indoor and outdoor environment, we need a system that monitors a volunteer to prevent dangerous situations and efficiently manages the data in real time. We developed a guidance device for visually impaired person that guides the user to walk safely to the destination in the previous study. We set a POI (Point of Interest) of a specific location indoors and outdoors and tracks the user's position and navigate the walking path using artificial markers and ZigBee modules as landmark. In addition, we develop path finding algorithm to be used for navigation in the guidance device. In the test bed, the volunteers are exposed to dangerous situations and can be an accident due to malfunction of the device since they are visually impaired person or normal person wearing a eye patch. Therefore the device requires a system that remotely monitors the volunteer wearing guidance device and manages indoor or outdoor a lot of map data. In this paper, we introduce a managing system that monitors the volunteers remotely and handles map data efficiently. We implement a management system which can monitor the volunteer in order to prevent a hazardous situation and effectively manage large amounts of data. In addition, we verified the effectiveness of the proposed system through various experiments.

• Journal of The Korean Association For Science Education
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• v.39 no.6
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• pp.755-765
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• 2019

This study aims to develop the scales to measure the innovation configurations of Science. Technology, Engineering, Art and Mathematics (STEAM) from the perspective of elementary school teachers and investigates the effect of the innovation configurations and related environmental factors on the usage levels of STEAM based on the newly developed scales for the innovation configurations of STEAM. The scales for the innovation configurations of STEAM are based on analyzing the various previous studies and in-depth interviews and consist of four sub-configurations: The 'Preparation,' 'Design,' 'Implementation,' and 'Evaluation.' The innovation configurations of STEAM was investigated with the developed scales through 266 teachers who are leading STEAM school teachers, are involved in STEAM research group, or are implementing STEAM in general schools. The final 19 questionnaires for the scale of the innovation configuration of STEAM are confirmed with exploratory factor analysis and reliability analysis. In order to examine the relationship between the innovation configurations and levels of use, the direct relationship between four sub-configurations and the usage levels of STEAM and the effect of environmental factors on the innovation configuration and the usage levels of STEAM have been conducted and their interactions are considered. The results show that 'Implementation' in the innovation configurations of STEAM and 'STEAM training experience' are most important factors to improve the usage levels of STEAM for elementary school teachers. It implies that the scales are very helpful in improving as well as figuring out the current innovation configurations of STEAM.

• Journal of Korea Water Resources Association
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• v.40 no.12
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• pp.985-994
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• 2007

Small hydropower is the one of the cleanest one among new and renewable energy with less green house gas emissions. Recently, the necessity of developing small hydropower is emerging since its remaining potential resources in this country are abundant. However, a survey or research on the small hydropower development has been hardly performed since the 1990#s. These circumstances encourages a systematic approach for the small hydropower development. The purpose of this study is to propose a methodology of the location analysis for developing small hydropower. To this end, constraint and location criteria with weights are established and quantification method of each factor is presented. Especially, the analysis procedure is established on the basis of GSIS. Also the study focus on raising the objectivity and precision of analysis by developing system model with automatic search. The proposed methodology is applied to Bochung stream in Keum Riverbasin. The result selects the four and two locations of dam type and run-of-river type respectively. This study will be beneficial to the future activation of small hydropower development as a fundamental work.

• Journal of Life Science
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• v.19 no.12
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• pp.1836-1840
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• 2009

Recently, hydroxymethylfurfrual (HMF) has been highlighted as a key intermediate for the production of liquid biofuels and other valuable compounds. We used sesame roots as a biocatalyst to synthesize HMF using flask cultures. The synthesis of HMF was identified by GC-mass analysis. The highest root growth was observed in cultures with 1.0 mg/l NAA at $30^{\circ}C$, while root growth was not found in those without NAA treatment. When silver nitrate ($AgNO_3$) was added, the root growth was greatest in those treated with 0.5 mg/l $AgNO_3$ and cultured at $30^{\circ}C$. In the case of HMF synthesis, its highest yield was obtained in those treated with 0.5 mg/l NAA at $25^{\circ}C$, but low HMF was detected in those treated without naphthaleneacetic acid (NAA). The addition of $AgNO_$ to the culture medium showed a 8-10% reduction in HMF yield compared to that of the control, indicating its inhibitory effect on the synthesis of HMF. On the whole, an optimal culture temperature for HMF synthesis seemed to be between $25-30^{\circ}C$.

• Science of Emotion and Sensibility
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• v.8 no.3
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• pp.177-187
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• 2005

This article examined how the amount of information presented in ad design affect consumer's evaluation on the ad Existing researches have mainly considered external information presented in the ad from the resource principle perspective. This study investigated how internal information processing such as imagery information processing caused by brand names moderates persuasion effects according to the amount of external information through two experiments. The experiments leave us the conclusion that brand names presented in the ads stimulate imagery information processing when surplus cognitive resources do exist and they lead ad recipients' evaluation to positive direction in prestige brand condition and to the contrary to negative direction in non-prestige brand condition. The results contribute to the direction of ad design in that ad needs not contain as muck product-related information as needed in order to increase persuasion effects in prestige brand condition. Rather, it's necessary to leave the room for internal information processing such as imagery information processing by well structured brand equity. On the contrary, non-prestige brand needs to contain explicit product-relevant information to exert a potent influence on a4 persuasion. We hope this study result is helpful for design direction of advertisement in order to heighten persuasion effects toward ad recipients.

• Journal of Digital Convergence
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• v.16 no.1
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• pp.135-146
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• 2018

This study aims to investigate the consumers' preference and willingness to pay for UWV panoramic video, which is a new realistic video technology, and propose the issues to be considered for developing and commercializing this technology in accordance with the needs of consumers. For this purpose, experimental study was conducted by constructing two environments; watching TV at home and watching panoramic video on the big screen. As a result, consumers in both experimental environments were more likely to prefer UWV than UHD, which is the existing video ratio. Consumers also showed intend to use UWV and are willing to pay for it. As a result of exploring the consumers' maximum amount of money to pay for the UWV and the reasons for it, the perception of immersion, liveliness, and actual experience of UWV were important factors for consumer acceptance of new realistic video technology.

• Microbiology and Biotechnology Letters
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• v.26 no.4
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• pp.331-337
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• 1998

The biological activities of both ethanol and water extracts from the fruiting body of E. applanata and E. applanata mycelium and the three fractions of ethanol extracts from E. applanata were compared. 91% of MCF7 cell growth was inhibited by adding 0.5 g/l of water extracts of E. applanata and 81% of MCF7 cell growth was inhibited by adding 0.5 g/l of diethyl ether and chloroform fractions. It was also showed that above 60% of Hep3B cell growth was inhibited by adding all samples including the fractions. The ethanol extracts of E. applanata mycelium showed 33.3% of cytotoxicity on normal liver cell, WRL68 in adding 0.5 g/l of the samples and 40% in adding 0.5 g/l of chloroform fractions. The result of anti-mutagenicity of all extracts and fractions including ethanol extracts of Phelinus linteus were showed that diethyl ether fractions were most effective than any other samples. Hypoglycemic activities of diethyl ether and chloroform fractions were the most effective which scores were above 75%. The enhancement of glutathione-S-transferase activity was increased above 2.3 times by adding 1.0 g/l ethanol extracts of E. applanata and diethyl ether, chloroform fractions. It can be concluded that both biological activities of the fruiting body and mycelium of E. applanata were almost equivalent.

• Journal of Digital Convergence
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• v.11 no.7
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• pp.271-279
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• 2013

This study has significance in terms of its intent to contribute to the increase in hospital revisits by proposing measures to enhance name awareness through an examination of the relationship between the name awareness of public medical institutions and patients' revisit, and its corresponding influences. The study findings were drawn by employing analysis methods such as frequency analysis, reliability and exploratory factor analysis, t-tests, ANOVA, correlation analysis, and hierarchical multiple regression analysis. The results of the correlation analysis showed statistically significant correlations between all independent variables and the patients' revisit or not. The perception of service quality and the environmental factor of medical treatment increased the explanatory power of independent variables, thereby exhibiting a high correlation with the revisit to medical institutions. The usual image, medical service, and medical treatment environment were revealed to have a positive influence on the patient's intent to revisit. This study could confirm a correlation between the name awareness of medical public institutions and the patients' revisit or not. Consequently, to improve the quality and infrastructure of their medical services, public medical institutions are required to perform studies on measures to increase the revisits of patients by identifying their image, medical services, and treatment environment on a consistent basis.