• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1248-1254
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• 2007

The secretory overexpression of Clostridium thermocellum endoglucanase A gene (celA) was examined in Saccharomyces cerevisiae using Kluyveromyces marxianus exoinulinase (INU1) signal sequence and GAL10 promoter. The two plasmids, pYEG-CT1 with its own signal sequence, and pYInu-CT1 with INU1 signal sequence were introduced to S. cerevisiae SEY2102 and S. cerevisiae 2805 host strains, respectively, and then each transformant was selected on the synthetic defined media lacking uracil. The expression level and secretion efficiency of endoglucanase A was increased by $18{\sim}22%$ and 11%, respectively, by INU1 signal sequence over celA signal sequence. By considering the high level of expression (361 unit/I), plasmid stability (89%), and secretion efficiency (70%), S. cerevisiae 2805 harboring plasmid pYInu-CT1 was selected as the opti-mal host vector system for the production of cellulose-degrading enzyme and recombinant yeast probiotic. The total expression and secretion efficiency of endoglucanase A was 418 unit/l and 73%, respectively, in the batch fermentation of S. cerevisiae 2805/pYlnu-CT1 on galactose medium. The mo-lecular weight of secreted endoglucanase A was found to be greater than 100 kDa, presumably due to the N-linked glycosylation.

• Journal of Navigation and Port Research
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• v.29 no.3 s.99
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• pp.189-194
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• 2005

The berthing/deberthing manoeuvring operation is the peculiar work owned to the marine pilot and the dock master. So, in the port or the shipyard, the berthing/deberthing manoeuvring operation requires considerable concentration and bears dangerousness. In that situation, a tug utilization is getting increased and the external forces have an effect on the own ship because of moving with low advance speed. In this study, we constructed the 2-dimensional virtual ship manoeuvring simulator system with which we can carry out the berthing/deberthing manoeuvring operation by using tugs in the external forces such as strong wind. And then, we propose the objective indexes by which the degree of manoeuvring difficulty evaluated. Using the present system, we carry out manoeuvring simulation experiment in order to grasp correlation between the objective indexes proposed here and the def{ree of manoeuvring difficulty felt by operator. Lastly, we discuss the evaluation technique of manoeuvring difficulty.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.06a
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• pp.183-185
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• 2014

The purpose of this paper aims at contributing to the national economic development through global competitiveness enhancement by marine finance's hub and marine logistics cluster by finance specialization and finance support as a creative-type service industry in global shipping port logistics. This study adopted the integrated approach and applied it to policy implementation to achieve the effectiveness. Creative-type marine finance development stages as a tool of policy implementation and the guide line for the time of policy implementation are followed by Stage 1(Construction & Growth Policy) for 2013~2016, Stage 2(Forstering & Activation Policy) for 2017~2019) and Stage 3(Continuous Development Policy) after 2020 until its completion. Korea has the inferiority over the competitiveness in global marine finance and needs a strategic approach to secure the liquidity of marine finance; interim, Islamic finance has been come to the force as a new alternative in financial transaction being accompanied by a spot transaction since the crisis of global finance. In order to create a potential slack of Korea in marine finance practice, in addition, this study suggests a consortium with the circle of Islamic finance as a clue of an easier policy implementation at the beginning stage.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.4
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• pp.436-442
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• 2011

To obtain the engine output correctly is basically very important factor for estimating a engine performance. But, it has been reported that the IHP measured from electronic indicator such as MIPS(Mean Indication Pressure System) has a deviation compared to mechanical indicator. It was reported by authors that the uncertainty of crank angle for TDC position could be one of the reasons. In this paper, the uncertainty of crank angle for TDC position and its influence to engine output were investigated respectively about M/E and G/E for marine diesel engines. For the purpose, two sampling methods of pressure in cylinder were considered which were 'angle base sampling' and 'time base sampling'. Angle base sampling is real crank angle acquired from angle encoder which is attached to crank shaft and time base sampling is crank angle calculated by detected revolution with Z-pluse of encoder. Time base sampling is same method of MIPS. This paper concluded that time base sampling method is not suitable for obtaining the output of marine diesel engine on board because of instantaneous speed variation and load fluctuation. Also it is verified that the variation of engine speed by load fluctuation should be one of reasons additionally in case of M/E.

• Applied Biological Chemistry
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• v.48 no.4
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• pp.431-434
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• 2005

The quantitative analytical method for major antioxidants, ellagic acid and punicalagin, in pomegranate husk (Granati pericarpium) were established by HPLC. The optimal HPLC conditions were as follows: Column; Agilent Zorbax Eclipse XDB-C18 ($4.6{\times}150mm,\;5{\mu}m$), mobile phase; 1% formic acid in water (A) and 1% formic acid in MeCN (B) (gradient elution of 5% to 100% B for 50 min), flow rate; 0.8 ml/min., detection; UV 254 nm. The optimal pre-treatment conditions for HPLC analysis were as follows: 5 g of pomegranate husk in 100 ml of 95% EtOH, refluxed for 3 h. Under these analytical conditions, punicalagin and ellagic acid contents in Korean pomegranates husks which were cultivated in five different sites were determined. As results, the ellagic acid and punicalagin (as a mixture of ${\alpha-\;and\;{\beta}-anomer$) contents were the highest in Haepyung pomegranate husk $(15.27{\mu}g/mg)$ and Jangsung pomegranate husk $(16.21{\mu}g/mg)$, respectively.

• Journal of Korean Society of Disaster and Security
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• v.6 no.3
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• pp.9-17
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• 2013

Geospatial information data came recently in use to build the location-based service in various fields. These data were shown via a 2-D map in the past but now can be viewed as a 3-D map due to the dramatic evolution of IT technology, thus improving efficiency and raising practicality to a greater extent by providing a more realistic visualization of the field. In addition, many previous GIS applications have been provided under desktop environment, limiting access from remote sites and reducing its approachability for less experienced users. The latest trend offers service with web-based environment, providing efficient sharing of data to all users, both unknown and specific internal users. Therefore, real-time information sensors that have been installed on steep slopes are to be integrated with 3-D geospatial information in this study. It is also to be developed with web-based environment to improve usage and access. There are three steps taken to establish this system: firstly, a 3-D GIS database and 3-D terrain with higher resolution aerial photos and DEM (Digital Elevation Model) have been built; secondly, a system architecture was proposed to integrate real-time sensor information data with 3D Web-based GIS; thirdly, the system has been constructed for Gangwon Province as a test bed to verify the applicability.

• Journal of Environmental Impact Assessment
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• v.27 no.5
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• pp.405-416
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• 2018

In this research, the distribution of Platinum Group Elements (PGEs) at roadside dust in Daejeon, Korea was examined using an ICP-MS (Inductively Coupled Plasma-Mass Spectrometry) technique. For the quality assurance of the determination, method validation based on its accuracy and precision was conducted using SRM (Standard Reference Material). It was found that the relative errors of Pt, Pd, and Rh against each SRM value were -0.7%, -10.0%, and -20.4%, respectively, while relative standard deviations for three elements were less than 10%. The concentrations of Pt, Pd and Rh in roadside dust averaged as $17.4{\pm}9.2{\mu}g/kg$, $283.6{\pm}20.5{\mu}g/kg$, and $7.3{\pm}2.8{\mu}g/kg$, respectively. The concentrations of Pt and Rh have significantly higher distribution patterns in the dust at roadside and underground parking lot than those in soil of the background or other urban area. The correlation analysis between concentrations of PGEs in roadside dust indicates that the distribution of Pt and Rh concentration were strongly affected by automobile sources.

• Proceedings of KOSOMES biannual meeting
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• 2003.11a
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• pp.157-162
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• 2003

The sludge oils were produced necessarily in the ships operation, so that it will be the best way to manage the sludge oils inside ship itself from a viewpoint of the prevention of marine oil pollution from ship. The ultrasonic breaking systems which recycle the sludge oil from ship into usable oil to be brunt is recognized as a most possible recycling device. In this regards, the purpose of this study is to examine erosion damage on the SS400 specimen by cavitation and the effect of impact pressure generated from the demolition of the cavity of ultrasonic vibration in the marine sludge oil environment .. The erosion damage of specimen was investigated mainly on weight loss, weight loss rate and maximum erosion rate with variation of the oil temperature as well as the change of space between transducer horn and specimen. The experimental results showed that as the space between ultrasonic vibrator horn and specimen disk increased, the weight loss and weight loss rate decreased and the values were larger in SFO than in SLO. The experimental results can be useful to the development of sludge oil disposing systems and to consider a countermeasure for the prevention of erosion damages by cavitation.

• Journal of Korea Multimedia Society
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• v.13 no.7
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• pp.1023-1043
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• 2010

As main memory get cheaper, it becomes increasingly affordable to load entire index of DBMS and to access the index. Since speed gap between CPU and main memory is growing bigger, many researches to reduce a cost of main memory access are under the progress. As one of those, cache conscious trees can reduce the cost of main memory access. Since cache conscious trees reduce the number of cache miss by compressing data in node, cache conscious trees can reduce the cost of main memory. Existing cache conscious trees use only fixed one compression technique without consideration of properties of data in node. First, this paper proposes the DC-tree that uses various compression techniques and change data layout in a node according to properties of data in order to reduce cache miss. Second, this paper proposes the level of compression locality that describes properties of data in node by formula. Third, this paper proposes Forced Partial Decomposition (FPD) that reduces the nutter of cache miss. DC-trees outperform 1.7X than B+-tree, 1.5X than simple prefix B+-tree, and 1.3X than pkB-tree, in terms of the number of cache misses. Since proposed DC-trees can be adopted in commercial main memory database system, we believe that DC-trees are practical result.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.5
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• pp.200-210
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• 2017

This Research is focused on ancient battle formation basing on the layout drawing of Yijinchongbang (manuals of learning military formations) in a strategy book in the late Joseon dynasty. The research topic is the principles of organic composition of battle formation and battlefield operating system by reforming the basic model of ancient battle formation. The research method is comparative analysis by reforming the battlefield operating system of types of disposition such as Obangjindisposition(Bangnjin; battle formation, Jikjin; direct battle formation, Gokjin; bend battle formation, Wonjin; round battle formation, and Yejin; keen battle formation), and Hyunmoojindo; turtle battle formation, Paljindo; all-rounder battle formation, Yookhwajindo; six flowers battle formation, Gugunjindo; nine forms battle formation. From the study results, Standoff Bombing of the battle formation in the late Joseon Dynasty basically started out from magic battle formation, but was then transformed into square, rectangle, pentagon, and circle. Also, the battle array composition used a 5-linear structure and was composed of 5 systems of circulation such as rectangle, square, diagonal, curve, and circle. The research findings elucidate the battlefield of the Joseon dynasty by establishing the real battle formation, and thus have military and academic value in suggesting possible tactics that can be used by modern training of military.