• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.115-115
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• 2011

본 연구에서는 자갈사주의 식생 이입 과정을 밝히고, 식생 이입에 영향을 미치는 수리적 특성을 규명하기 위한 현지조사 방안을 제시하였다. 자갈사주의 식생이입은 조절 하천과 비조절 하천에서의 이입 과정이 다르며, 본 연구에서는 비조절 하천을 대상으로 하였다. 따라서 상류에 댐이나 저수지가 없는 자연에 가까운 하천을 고려하였으며, 장기적으로는 조절 하천의 식생이입 과정과의 비교를 위해 임하댐의 반변천과 가까운 길안천의 중류 구간을 선정하였다. 자갈사주의 식생이입 과정을 모니터링하기 위해, 자갈사주를 전구역과 세부구역으로 나누어 같은 지점에서 같은 배율로 사진을 촬영하였다. 전자는 자갈사주 전체를 대상으로 촬영하며, 이를 위한 view point 선정이 중요하고 선정에 적절한 지점을 제시하였다. 후자의 경우 식생의 이입 정도를 왕성, 보통, 미약, 없음(4단계)로 구분하기 위해 대상 지점을 matrix가 50% 이상인 지점 ; 식생이입 왕성 (2개 지점), matrix가 50% 이하인 지점 ; 식생이입 보통, 또는 미약 (2개 지점), matrix가 없는 자갈만의 지점 ; 식생이입 없음 (1개 지점)을 선정하였다. 식생이입에 영향을 미치는 변수로는 하상토의 입경과 흐름의 특성(수심, 유속, 하상 소류력 등)을 들 수 있으며, 이들 조사를 위한 방법을 제시하였다.

• Korean Journal of Child Studies
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• v.14 no.2
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• pp.137-147
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• 1993

감정이입은 친사회적 행동의 잠재적 동기요인으로서 발달심리학자들 (e.g., Feshbach, 1978 ; Hoffman, 1975)로부터 지대한 관심을 받아왔다. 어려움에 처한 다른 사람에 대해 감정이입적으로 자극된 사람은 상호 체험하는 심적 고통(distress)으로부터 벗어난다는 기대에 의해서, 또는 지원적 행위 후에 대리체험할 수 있는 긍정적인 감정에 대한 기대에 의해서, 그 사람을 지원하도록 동기유발 되어질 수 있다(Barnett & Thompson, 1985; Hoffman, 1975). 감정이입과 친사회적 행동 사이의 긍정적 관계에 대한 충분한 이론적 근거에도 불구하고, 그 관련성을 실증하는데 실패한 많은 연구들에서 나타난 개념적, 방법론적 문제들이 본 논문에서 분석되었다. 성인의 감정이입과 친사회적 행동과의 관계에 대한 연구들은 상당히 일관된 긍정적 결과를 제시해온 반면, 아동들, 특히 어린 유아들을 대상으로 한 경우, 명백하거나 쉽게 해석할 수 있는 패턴의 관련성이 확립되지 못했다. 이러한 종전 연구에서의 문제점은 감정이입에 대한 개념적 논쟁 및 측정방법의 어려움에 기인할 수 있다. 감정이입과 친사회적 행동 사이의 실험적인 관련성의 강도는 이 변인들을 측정하기 위해 사용된 방법들의 특성과 제한성에 다분히 의존하는 것으로 보인다. 친사회적 반응에 영향을 미치는 것으로 가정되어지는 다양한 상황적, 동기적 요소들을 감안하여, 유아의 감정이입 능력이 구체적인 사회적 상황에서 작용되는 과정이 보다 면밀하게 연구되어져야 한다.

• Tuberculosis and Respiratory Diseases
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• v.41 no.2
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• pp.87-96
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• 1994

Background : Since tumor necrosis factor was discovered in 1975, TNF has been well known about its cytotoxic effect on tumor cells in vivo and in vitro. According to the recent improvement of molecular biological techinques, it is possible that exogenous TNF gene is transferred to tumor cells and is expressed in theirs. By virtue of TNF gene transfer, we have expected that TNF expressed in TNF-gene-transferred tumor cells would kill tumor cells in vivo without systemic side effect. The expected mechanisms in which antitumor effects of TNF expressed in TNF-gene-transferred tumor cells are working would be as followings. In the first mechanism, TNF expressed in TNF-gene-transferred tumor cells would kill tumor cells around(like homicide). In the second mechanism, TNF expressed in TNF-gene-transferred tumor cells would kill themselves(like suicide). In the third mechanism, TNF expressed in TNF-gene-transferred tumor cells would recruit immune effector cells and kill tumor cells indirectly. In the last mechanism, TNF expressed in TNF-gene-transferred tumor cells would augment cytokine such as interferon-$\gamma$ to kill tumor cells. Among these four mechanisms of antitumor effect, only the second mechanism has not been established yet. Therefore, to elucidate the second mechanism, We performed this study. Method : We transferred TNF-$\alpha$ gene to NCI-H2058, a human mesothelioma cell line and WEHI164, a murine fibrosarcoma cell line by using retroviral vector(pLT12SNTNF). And, We determined by using MTT assay whether TNF expressed in TNF-gene-transferred tumor cell lines would kill themselves like suicide or not. Then, if TNF-gene-transferred tumor cell lines would not suicide themselves, I would know more about the TNF sensitivity of TNF-gene-transferred tumor cell lines to exogenous TNF also by MTT assay. Result : NCI-H2058 and WEHI164 which were sensitive to TNF, became far less sensitive to endogenous and exogenous TNF after being transferred TNF-$\alpha$ gene to. Conclusion : TNF-gene-transfer to NCI-H2058 and WEHI164 gave them resistance to TNF.

• Cartoon and Animation Studies
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• s.15
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• pp.179-192
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• 2009

This study investigates the effects of empathy responses towards animated spokes-characters in advertising on brand likability. This study also examines the mediating role of cognitive and affective responses towards character advertising in the effect process of empathy. Statistically, several significant results are found. First, empathy has positive effects on cognitive responses and affective responses. Second, both cognitive and affective responses have positive influence on brand likability, but the effects of cognitive responses show relatively weaker than those of affective responses. Third, empathy has a positive influence on brand likability mediated by cognitive responses, but affective responses as a mediator do not show statistically significant differences. The results of this study indicate consumers can have emotional responses to advertising characters just as they do to human information sources. And the results also imply that enhancement of the empathy intensity should be considered to make effective animated characters in advertising, and that advertising appeals should be practiced to form cognitive responses positively and strongly to strengthen consumers' empathy responses.

• Tuberculosis and Respiratory Diseases
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• v.44 no.6
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• pp.1353-1365
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• 1997

Background : Tumor necrosis factor(TNF) has been considered as an important candidate for cancer gene therapy based on its potent anti-tumor activity. However, since the efficiency of current techniques of gene transfer is not satisfactory, the majority of current protocols is aiming the in vitro gene transfer to cancer cells and re-introducing genetically modified cancer cells to host. In the previous study, it was shown that TNF-sensitive cancer cells transfected with TNF-$\alpha$ cDNA would become highly resistant to TNF, and the probability was shown that the acquired resistance to TNF might be associated with synthesis of some protective protein. Understanding the mechanisms of TNF -resistance in TNF-$\alpha$ cDNA transfected cancer cells would be. an important step for improving the efficacy of cancer gene therapy as well as for better understandings of tumor biology. This study was designed to evaluate the role of MnSOD, an antioxidant enzyme, in the acquired resistance to TNF of TNF-$\alpha$ cDN A transfected cancer cells. Method : We transfected TNF-$\alpha$ c-DNA to WEHI164(murine fibrosarcoma cell line), NCI-H2058(human mesothelioma cell line), A549(human non-small cell lung cancer cell line), ME180(human cervix cancer cell line) cells using retroviral vector(pLT12SN(TNF)) and confirm the expression of TNF with PCR, ELISA, MIT assay. Then we determined the TNF resistance of TNF-$\alpha$ cDNA transfected cells(WEHI164-TNF, NCIH2058-TNF, A549-TNF, ME180-TNF) and the changes of MnSOD mRNA expressions with Northern blot analysis. Results : The MnSOD mRNA expressions of parental cells and genetically modified cells of WEHI164 and ME180 cells(both are naturally TNF sensitive) were not significantly different The MnSOD mRNA expressions of genetically modified cells of NCI-H2058 and A549(both are naturally TNF resistant) were higher than those of the parental cells, while those of parental cells with exogenous TNF were also elevated. Conclusion : The acquired resistance to TNF after TNF-$\alpha$ cDNA transfection may not be associated with the change in the MnSOD expression, but the difference in natural TNF sensitivity of each cell may be associated with the level of the MnSOD expression.

• KSBB Journal
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• v.14 no.3
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• pp.377-379
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• 1999

In order to improve the transfection efficiency of CHO/dhfr- cells using cationic lipid, optimal concentrations of the cationic lipid($LipofectAmine^{TM}$) and DNA(pEGFP-C1) need to be determined. The use of green fluorescent protein(GFP) gene as a reporter gene facilitated the quantification of transfection efficiency. The green fluorescence intensity of each cell transfected at various lipid-DNA concentrations was measured using fluorescence-activated cell sorter(FACS) analysis. A combination of $2.0{\mu}L$ cationic lipid and 0.4{$\mu}g$ DNA in a well resulted in the highest trasfection efficiency. Taken together, the method using FACS analysis of GFP is simple and fast, facilitating the optimization of transfection.

• Radiation Oncology Journal
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• v.19 no.4
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• pp.381-388
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• 2001

Purpose : This study was conducted to assess the effects of x-irradiation on the expression of the novel glutathione S-transferase K1 gene. Materials and methods : Human glutathione S-transferase K1 (hGSTK1) DNA was purified and ligated to a pcDNA3.1/Myc-His(+) vector for the overexpression of hGSTK1 gene. MCF-7 cells were transfected with or without the recombinant hGSTK1 gene, and irradiated with 6 MV x-ray. After incubation of 14 days, cell survival was measured and compared. The expression of hGSTK1 and the effect of x-irradiation on hGSTK1 expression were also estimated in MCF-7 cells transfected with or without the hGSTK1 gene by RT-PCR. Results : Following 2 to 12 Gy of x-irradiation, the cell survivals were higher in the MCF-7 cells transfected with the hGSTK1 gene than in those without transfection. Despite the higher cell survival in the hGSTK1-transfected cells, RT-PCR for hGSTK1 mRNA revealed no significant differences according to radiation dose, fractionation, and time after irradiation. Conclusion : The MCF-7 cells transfected with the hGSTK1 gene showed higher cell survival than those without transfection of the gene. The hGSTK1 gene might be associated with the radiosensitivity of MCF-7 cell line and further analysis should be needed.

• Korean Journal of Social Welfare Studies
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• no.37
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• pp.145-170
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• 2008

This qualitative study explores social workers' phenomenological experiences with battered women, including their struggles and needs for building and maintaining an empathic identification with their battered women clients. Twelve interviews were conducted with social workers who have provided services in the domestic violence field over the last two years. They identified their clients prejudices and unfamiliarity with counseling and social work profession in Korea as related to battered women clients' initial resistance to social workers' empathic engagement. The nature of victimization between intimate partners (i.e., emotional bounds, continuing risks of victimization) requires workers' enormous energy to maintain their need for an empathic identification with their clients. Social workers emphasized the importance of clinical supervision and supportive networks that enable them to maintain their professional energy and commitment as an empathic helper.

• Nuclear Medicine and Molecular Imaging
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• v.42 no.5
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• pp.383-393
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• 2008

Purpose: Cancer specific killing can be achieved by therapeutic gene activated by cancer specific promotor. Expression of sodium iodide symporter (NIS) gene causes transportation and concentration of iodide into the cell, therefore radioiodine treatment after NIS gene transfer to cancer cell could be a form of radionuclide gene therapy. luciferase (Luc) gene transfected cancer cell can be monitored by in vivo optical imaging after D-luciferin injection. Aims of the study are to make vector with both therapeutic NIS gene driven by AFP promoter and reporter Luc gene driven by CMV promoter, to perform hepatocellular carcinoma specific radiodiodine gene therapy by the vector, and assessment of the therapy effect by optical imaging using luciferase expression. Materials and Methods: A Vector with AFP promoter driven NIS gene and CMV promoter driven Luc gene (AFP-NIS-CMV-Luc) was constructed. Liver cancer cell (HepG2, Huh-7) and non liver cancer cell (HCT-15) were transfected with the vector using liposome. Expression of the NIS gene at mRNA level was elucidated by RT-PCR. Radioiodide uptake, perchlorate blockade, and washout tests were performed and bioluminescence also measured by luminometer in these cells. In vitro clonogenic assay with 1-131 was performed. In vivo nuclear imaging was obtained with gamma camera after 1-131 intraperitoneal injection. Results: A Vector with AFP-NIS-CMV-Luc was constructed and successfully transfected into HepG2, Huh-7 and HCT-15 cells. HepG2 and Huh-7 cells with AFP-NIS-CMV-Luc gene showed higher iodide uptake than non transfected cells and the higher iodide uptake was totally blocked by addition of perchlorate. HCT-15 cell did not showed any change of iodide uptake by the gene transfection. Transfected cells had higher light output than control cells. In vitro clonogenic assay, transfected HepG2 and Huh-7 cells showed lower colony count than non transfected HepG2 and Huh-7 cells, but transfected HCT-15 cell did not showed any difference than non transfected HCT-15 cell. Number of Huh-7 cells with AFP-NIS-CMV-Luc gene transfection was positively correlated with radioidine accumulation and luciferase activity. In vivo nuclear imaging with 1-131 was successful in AFP-NIS-CMV-Luc gene transfected Huh-7 cell xenograft on nude mouse. Conclusion: A Vector with AFP promoter driven NIS and CMV promoter driven Luc gene was constructed. Transfection of the vector showed liver cancer cell specific enhancement of 1-131 cytotoxicity by AFP promoter, and the effect of the radioiodine therapy can be successfully assessed by non-invasive luminescence measurement.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.196-196
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• 2011

본 연구에서는 일본 다마천 자갈사주의 식생 이입 과정을 조사하고, 과도한 식생 이입에 영향을 미치는 하상토의 특성과 수리적 특성을 분석하여 자갈사주의 복원을 위한 방안에 관하여 조사하였다. 다마천 자갈사주는 넓게 분포하였던 자갈하천(white river)에 버드나무 등의 식생이 과다하게 성장하여(green river), 이를 억제하기 위한 대책을 수립하여 시행하였다. 또한 이곳에 서식하는 고유종(야생 국화)를 복원하기 위한 연구도 진행되었다. 다마천 자갈사주 식생번무의 원인으로, 자갈과 모래 채취, 하천 개수, 상류의 댐과 보의 축조를 들 수 있으며, 자갈사주를 복원하기 위한 종합적인 연구계획이 수립되어 사업이 시행되었다. 복원 대책으로 홍수터를 파고 모래톱 복원, 상류로부터 자갈과 모래 공급, 저수로 확장, 과다 성장한 버드나무나 아카시아 벌목, 표토 제거를 계획하였으며 이를 시행하였다. 모래톱은 성긴 조약돌로 포설하였으며, 다양한 높이로 조성하여 침수 빈도를 다양하게 변화시켰다. 가장 높은 모래톱은 5년 빈도로 하였다. 자갈사주의 복원 및 관리를 위한 모니터링 그룹도 발족하여 현재 자갈사주의 복원 모니터링이 진행 중이며, 이를 바탕으로 체계적이고 계획적인 복원 사업이 진행되고 있다.