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Molecular Breeding of Tobacco Plants Resistant to TMV and PVY (분자생물학적 TMV 및 PVY 저항성 연초 육종)

  • E.K. Pank;Kim, Y.H.;Kim, S.S.;Park, S.W.;Lee, C.H.;K.H.Paik
    • Proceedings of the Korean Society of Tobacco Science Conference
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    • 1997.10a
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    • pp.134-152
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    • 1997
  • Plant viruses of tobacco including tobacco mosaic virus (TMV) and potato virus Y (PVY) cause severe economic losses in leaf-tobacco production. Cultural practices do not provide sufficient control against the viruses. Use of valuable resistant cultivars is most recommendable for the control of the viruses. However, conventional breeding programs are not always proper for the development of virus-resistant plants mostly owing to the frequent lack of genetic sources and introduction of their unwanted properties. Therefore, we tried to develop virus-resistant tobacco plants by transforming commercial tobacco cultivars, NC 82 and Burley 21, with coat protein (CP) or replicase (Nlb) genes of TMV and PVY necrosis strain (PVY-VN) with or without untranslated region (UTR) and with or without mutation. Each cDNA was cloned and inserted in plant expression vectors with 1 or 2 CaMV 35S promotors, and introduced into tobacco leaf tissues by Agrobacterium tumefaciens LBA 4404. Plants were regenerated in kanamycin-containing MS media. Regenerated plants were tested for resistance to TMV and PVY In these studies, we could obtain a TMV-resistant transgenic line transformed with TMV CP and 6 genetic lines with PVY-VN cDNAs out of 8 CP and replicase genes. In this presentation, resistance rates, verification of gene introduction in resistant plants, stability of resistance through generations, characteristics of viral multiplication and translocation in resistant plants, and resistance responses relative to inoculum potential and to various PVY strains will be shown. Yield and quality of leaf tobacco of a promising resistant tobacco line will be presented.

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Contamination level and congener profiles of PCBs, Co-PCBs and PCDD/DFs in transformer insulation oil samples (변압기 절연유 중 PCBs, Co-PCBs 및 PCDD/PCDFs 오염수준 및 이성체 분포)

  • Kim, Kyoung-Soo;Kim, Jong-Guk;Shin, Sun-Kyoung;Kim, Kyoung-Sim;Song, Byung-Joo
    • Analytical Science and Technology
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    • v.19 no.3
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    • pp.263-271
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    • 2006
  • The levels of total PCBs, Co-PCBs and PCDD/Fs in the transformer insulation oil samples obtained using GC/ECD and HRGC/HRMS were ranged from N.D. to 77.3 ppm, from 0.0863 to 2.49 ppm and from N.D. to 0.00241 ppm, respectively. In terms of WHO-TEQ values, Co-PCBs and PCDD/Fs were ranged from 23.3 to 600 pgTEQ/g and from N.D. to 128 pgTEQ/g, respectively (${\Sigma}Co$-PCBs+PCDD/Fs concentration was calculated 24.4~728 pgTEQ/g). Although, the contribution of PCDD/Fs was below 12% in total TEQ concentration, it is suggested contamination of PCDD/Fs in transformer insulation oils. Among 10 samples, 4 samples showed higher concentration than 2 ppm (specific waste criterion of Korea) and Aroclor 1242, 1248, 1254 and 1260 was detected in samples as a single or mixture of Aroclor. It was shown reliable relationship between concentration of Co-PCBs and those of PCDD/Fs (p<0.003), however, was not shown between production year of transformer and concentration of PCBs. The distribution pattern of Co-PCB congeners showed that the ratios of mono-ortho substituted congeners were higher than non-ortho substituted congeners. Among that, PCB-118 congener was predominant. In addition, the OCDD congener was predominated in PCDD/Fs congeners as above 53%. Moreover, the congener pattern of Co-PCBs was similar to that of Aroclor as well as ambient air, which suggested that PCBs volatilization from transformer insulation oil affected the pattern of Co-PCBs in ambient air.

A Study on change in thermal properties and chemical structure of Zr-Ni delay system by aging (노화에 따른 Zr-Ni계 지연관의 열 특성 및 화학적 구조 변화에 관한 연구)

  • Park, Byung Chan;Chang, Il Ho;Kim, Sun Tae;Hwang, Taek Sung;Lee, Seungho
    • Analytical Science and Technology
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    • v.22 no.4
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    • pp.285-292
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    • 2009
  • It has been observed that, after long term storage, some ammunitions are misfired by tamping (combustionstopping) due to aging of the chemicals loaded in the ammunitions. Used in ammunitions are percussion powder which provides the initial energy, igniter which ignites the percussion powder, and a delay system that delays the combustion for a period of time. The percussion powder is loaded first, followed by the igniter and then the delay system, and the ammunitions explode by the energy being transferred in the same order. Tamping occurs by combustion-stopping of the igniter or insufficient energy transfer from the igniter to the delay system or the combustion-stopping of the delay system, which are suspected to be caused by low purity of the components, inappropriate mixing ratio, size distribution of particulate components, type of the binder, blending method, hydrolysis by the humidity penetrated during the long term storage, and chemical changes of the components by high temperature. Goal of this study is to find the causes of the combustion-stopping of the igniter and the delay system of the ammunitions after long term storage. In this study, a method was developed for testing of the combustion-stopping, and the size distributions of the particulate components were analyzed with field-flow fractionation (FFF), and then the mechanism of chemical change during long term storage was investigated by thermal analysis (differential scanning calorimetry), XRD (X-ray diffractometry), and XPS (X-ray photoelectron spectroscopy). For the ignition system, M (metal)-O (oxygen) and M-OH peaks were observed at the oxygen's 1s position in the XPS spectrum. It was also found by XRD that $Fe_3O_4$ was produced. Thus it can be concluded that the combustion-stopping is caused by reduction in energy due to oxidation of the igniter.

Contents of mercury, lead, cadmium, and arsenic in dried marine products (건포류의 수은, 납, 카드뮴 및 비소 함유량)

  • Ok, Hwang Young;Kim, Su Un;Ryu, Seung Hee;Ham, Hee Jin;Park, Gun Yong;Park, Seog Gee
    • Analytical Science and Technology
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    • v.22 no.4
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    • pp.336-344
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    • 2009
  • This survey was carried out to estimate the contents of heavy metals (Hg, Pb, Cd, and As) in 10 kinds of dried marine products (n=189) which are closely related to food resources. The contents of heavy metals were estimated by inductively coupled plasma spectrometer (ICP) and mercury analyzer. The values of heavy metals in dried marine products were as follows (Mean${\pm}$SD (range), mg/kg). The average contents of heavy metals in the dried marine products were Hg $0.058{\pm}0.069$ (0.002~0.502) mg/kg, Pb $0.178{\pm}0.598$ (ND~5.130) mg/kg, Cd $0.306{\pm}0.610$ (ND~6.802) mg/kg, As $5.282{\pm}6.158$ (ND~71.760) mg/kg. The range of heavy metal contents in dried marine products are low level, except of lead contents (n=2). In the comparison of heavy metal content by anchovy size, it was shown that cadmium, mercury and arsenic were meaningfully different. The contents of heavy metals by the shape of shrimp and Alaska Pollack were shown that the result of each group was meaningless. In the comparison imported production with domestic production, in the case of common squid, cadmium and arsenic were meaningfully different. The weekly average intakes of Hg, Pb and Cd from dried marine products takes about 1.17~11.52% of PTWI (Provisional Tolerable Weekly Intakes) that FAO/WHO Joint Food Additive and Contaminants Committee has set to evaluate their safeties.

Usefulness of Canonical Correlation Classification Technique in Hyper-spectral Image Classification (하이퍼스펙트럴영상 분류에서 정준상관분류기법의 유용성)

  • Park, Min-Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.5D
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    • pp.885-894
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    • 2006
  • The purpose of this study is focused on the development of the effective classification technique using ultra multiband of hyperspectral image. This study suggests the classification technique using canonical correlation analysis, one of multivariate statistical analysis in hyperspectral image classification. High accuracy of classification result is expected for this classification technique as the number of bands increase. This technique is compared with Maximum Likelihood Classification(MLC). The hyperspectral image is the EO1-hyperion image acquired on September 2, 2001, and the number of bands for the experiment were chosen at 30, considering the band scope except the thermal band of Landsat TM. We chose the comparing base map as Ground Truth Data. We evaluate the accuracy by comparing this base map with the classification result image and performing overlay analysis visually. The result showed us that in MLC's case, it can't classify except water, and in case of water, it only classifies big lakes. But Canonical Correlation Classification (CCC) classifies the golf lawn exactly, and it classifies the highway line in the urban area well. In case of water, the ponds that are in golf ground area, the ponds in university, and pools are also classified well. As a result, although the training areas are selected without any trial and error, it was possible to get the exact classification result. Also, the ability to distinguish golf lawn from other vegetations in classification classes, and the ability to classify water was better than MLC technique. Conclusively, this CCC technique for hyperspectral image will be very useful for estimating harvest and detecting surface water. In advance, it will do an important role in the construction of GIS database using the spectral high resolution image, hyperspectral data.

Structural Evaluation Method to Determination Safe Working Load of Block Handling Lugs (블록 이동용 러그의 안전사용하중 결정에 관한 구조 평가법)

  • O-Hyun Kwon;Joo-Shin Park;Jung-Kwan Seo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.4
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    • pp.363-371
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    • 2023
  • To construct a ship, blocks of various sizes must be moved and erected . In this process, lugs are used such that they match the block fastening method and various functions suitable for the characteristics of each shipyard facility. The sizes and shapes of the lugs vary depending on the weight and shape of the block structures. The structure is reinforced by welding the doubling pads to compensate for insufficient rigidity around the holes where the shackle is fastened. As for the method of designing lugs according to lifting loading conditions, a simple calculation based on the beam theory and structural analysis using numerical modeling are performed. In the case of the analytical method, a standardized evaluation method must be established because results may differ depending on the type of element and modeling method. The application of this ambiguous methodology may cause serious safety problems during the process of moving and turning-over blocks. In this study , the effects of various parameters are compared and analyzed through numerical structural analysis to determine the modeling conditions and evaluation method that can evaluate the actual structural response of the lug. The modeling technique that represents the plate part and weld bead around the lug hole provides the most realistic behavior results. The modeling results with the same conditions as those of the actual lug where only the weld bead is connected to the main body of the lug, showed a lower ulimated strength compared with the results obtained by applying the MPC load. The two-dimensional shell element is applied to reduce the modeling and analysis time, and a safety working load was verified to be predicted by reducing the thickness of the doubling pad by 85%. The results of the effects of various parameters reviewed in the study are expected to be used as good reference data for the lug design and safe working load prediction.

Impact of Emotional Regulation on the Quality of Life in Elderly People (노인의 감정조절이 삶의 질에 미치는 영향)

  • Yun, EunGyeong;Jo, YeunDuk
    • 한국노년학
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    • v.30 no.4
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    • pp.1429-1444
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    • 2010
  • The purpose of this study was to examine the impact of elderly people's difficulties in emotional regulation on their quality of life and to suggest possible ways of improving their emotional regulation. The subjects in this study were 345 senior citizens who participated in community education programs and used senior centers. A survey was conducted in person, and the instrument used to check their difficulties in emotional regulation was Gratz & Roemer(2004)'s inventory that was rearranged to serve the purpose of this study. When a factor analysis was carried out, their emotional regulation difficulties were categorized into five factors, which were respectively named troubles in emotional response handling, difficulties in accurate emotional awareness, difficulties in emotional coping, and difficulties in emotional reception. The findings of the study were as follows: First, the senior citizens were different from one another in emotional regulation difficulties according to their personal characteristics involving gender, income, financial state, hospitalization experience over the past three months, and presence or absence of disease. Second, their quality of life significantly varied with gender, age, presence or absence of spouse, form of residence, education, income, financial state, hospitalization experience and presence or absence of disease. Third, as a result of investigating the influence of their emotional regulation difficulties on the overall quality of life, a better quality of life was found among those who were male and who had an income and suffered from fewer diseases. And a lower quality of life was found among the senior citizens who faced difficulties in emotional response handling, who had difficulties in emotional control and who lagged behind in terms of emotional coping. Accordingly, the emotional regulation difficulties of the senior citizens could be said to be closely linked to their quality of life. Given the findings of the study, in which way elderly people could be helped to improve their emotional regulation in consideration of their own personal characteristics was discussed, and how to classify their emotional regulation difficulties from various angles to relieve them of the troubles was suggested.

Assessment of stream water quality and pollutant discharge loads affected by recycled irrigation in an agricultural watershed using HSPF and a multi-reservoir model (HSPF와 다중 저류지 모형을 이용한 농업지역 순환관개에 의한 하천 수질 및 배출부하 영향 분석)

  • Kyoung-Seok Lee;Dong Hoon Lee;Youngmi Ahn;Joo-Hyon Kang
    • Journal of Wetlands Research
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    • v.25 no.4
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    • pp.297-305
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    • 2023
  • The recycled irrigation is a type of irrigation that uses downstream water to fulfill irrigation demand in the upstream agricultural areas; the used irrigation water returns back to the downstream. The recycled irrigation is advantageous for securing irrigation water for plant growth, but the returned water typically contains high levels of nutrients due to excess nutrients inputs during the agricultural activities, potentially deteriorating stream water quality. Therefore, quantitative assessment on the effect of the recycled irrigation on the stream water quality is required to establish strategies for effective irrigation water supply and water quality management. For this purpose, a watershed model is generally used; however no functions to simulate the effects of the recycled irrigation are provided in the existing watershed models. In this study, we used multi-reservoir model coupled with the Hydrological Simulation Program-Fortran (HSPF) to estimate the effect of the recycled irrigation on the stream water quality. The study area was the Gwangok stream watershed, a subwatershed of Gyeseong stream watershed in Changnyeong county, Gyeongsangnam-do. The HSPF model was built, calibrated, and used to produce time series data of flow and water quality, which were used as hypothetical observation data to calibrate the multi-reservoir model. The calibrated multi-reservoir model was used for simulating the recycled irrigation. In the multi-reservoir model, the Gwangok watershed consisted of two subsystems, irrigation and the Gwangok stream, and the reactions (plant uptake, adsorption, desorption, and decay) within each subsystem, and fluxes of water and materials between the subsystems, were modeled. Using the developed model, three scenarios with different combinations of the operating conditions of the recycled irrigation were evaluated for their effects on the stream water quality.

Estimation of Rice Heading Date of Paddy Rice from Slanted and Top-view Images Using Deep Learning Classification Model (딥 러닝 분류 모델을 이용한 직하방과 경사각 영상 기반의 벼 출수기 판별)

  • Hyeok-jin Bak;Wan-Gyu Sang;Sungyul Chang;Dongwon Kwon;Woo-jin Im;Ji-hyeon Lee;Nam-jin Chung;Jung-Il Cho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.337-345
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    • 2023
  • Estimating the rice heading date is one of the most crucial agricultural tasks related to productivity. However, due to abnormal climates around the world, it is becoming increasingly challenging to estimate the rice heading date. Therefore, a more objective classification method for estimating the rice heading date is needed than the existing methods. This study, we aimed to classify the rice heading stage from various images using a CNN classification model. We collected top-view images taken from a drone and a phenotyping tower, as well as slanted-view images captured with a RGB camera. The collected images underwent preprocessing to prepare them as input data for the CNN model. The CNN architectures employed were ResNet50, InceptionV3, and VGG19, which are commonly used in image classification models. The accuracy of the models all showed an accuracy of 0.98 or higher regardless of each architecture and type of image. We also used Grad-CAM to visually check which features of the image the model looked at and classified. Then verified our model accurately measure the rice heading date in paddy fields. The rice heading date was estimated to be approximately one day apart on average in the four paddy fields. This method suggests that the water head can be estimated automatically and quantitatively when estimating the rice heading date from various paddy field monitoring images.

Observation of Methane Flux in Rice Paddies Using a Portable Gas Analyzer and an Automatic Opening/Closing Chamber (휴대용 기체분석기와 자동 개폐 챔버를 활용한 벼논에서의 메탄 플럭스 관측)

  • Sung-Won Choi;Minseok Kang;Jongho Kim;Seungwon Sohn;Sungsik Cho;Juhan Park
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.436-445
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    • 2023
  • Methane (CH4) emissions from rice paddies are mainly observed using the closed chamber method or the eddy covariance method. In this study, a new observation technique combining a portable gas analyzer (Model LI-7810, LI-COR, Inc., USA) and an automatic opening/closing chamber (Model Smart Chamber, LI-COR, Inc., USA) was introduced based on the strengths and weaknesses of the existing measurement methods. A cylindrical collar was manufactured according to the maximum growth height of rice and used as an auxiliary measurement tool. All types of measured data can be monitored in real time, and CH4 flux is also calculated simultaneously during the measurement. After the measurement is completed, all the related data can be checked using the software called 'SoilFluxPro'. The biggest advantage of the new observation technique is that time-series changes in greenhouse gas concentrations can be immediately confirmed in the field. It can also be applied to small areas with various treatment conditions, and it is simpler to use and requires less effort for installation and maintenance than the eddy covariance system. However, there are also disadvantages in that the observation system is still expensive, requires specialized knowledge to operate, and requires a lot of manpower to install multiple collars in various observation areas and travel around them to take measurements. It is expected that the new observation technique can make a significant contribution to understanding the CH4 emission pathways from rice paddies and quantifying the emissions from those pathways.