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Microbial community analysis of an eco-friendly recirculating aquaculture system for olive flounder (Paralichthys olivaceus) using complex microbial probiotics (복합미생물 프로바이오틱을 이용한 환경친화적 넙치 순환여과양식시스템에서의 미생물군집 분석)

  • Rhee, Chaeyoung;Kim, Haham;Emmanuel, S. Aalfin;Kim, Hong-Gi;Won, Seonghun;Bae, Jinho;Bai, Sungchul C.;Koh, Sung-Cheol
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.369-378
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    • 2018
  • This study was conducted to evaluate effects of dietary microbial probiotics on the growth and disease resistance of olive flounder (Paralichthys olivaceus) in a recirculating aquaculture system (RAS), and the effects of the probiotic bioaugmentation on the microbial community structure and water quality. For the analysis, 80 juvenile fish (average weight, $25.7{\pm}7.6g$; average length, $15.2{\pm}1.7cm$) were fed a basal diet containing a commercial microbial product CES-AQ1 (CES; $1{\times}10^9\;CFU/kg$ diet) in an RAS for 8 weeks. Weight gain, the specific growth rate, feed efficiency, and protein efficiency ratio of the fish fed the CES diet in the RAS were 1.5~2.5 times higher than those of fish fed the basal diet alone, or the basal diet containing oxytetracycline (OTC), yeast plus bacterium, or Bacillus subtilis in a still water system. There was no significant difference in the pathogen challenge test between fish fed the OTC diet and fish fed the CES diet in the RAS, suggesting the CES-AQ1 probiotic used in the RAS as a potential replacement for antibiotics. The RAS biofilter maintained the highest microbial diversity and appeared to harbor microbial communities with ammonium oxidation, denitrification, and fish pathogen suppression functions. Ammonia, which is hazardous to fish, was significantly decreased to < 0.5 mg/L in 19 days, indicating the effectiveness of probiotic supplementation to maintain good water quality in RAS. These results suggest that the intestinal microbial communities of fish are stabilized by a probiotic-containing diet (CES) and that bioaugmentation with probiotics may be an eco-friendly and economical supplement for aquaculture of olive flounder, promoting both good water quality and fish health in an RAS.

A Study on the Characteristics of PM1.0 Chemical Components Using a Real-time Aerosol Mass Spectrometer (실시간 에어로졸 질량분석기를 이용한 PM1.0의 화학적성분의 특성에 관한 연구)

  • Park, Jinsoo;Choi, Jinsoo;Kim, Hyunjae;Oh, Jun;Sung, Minyoung;Ahn, Joonyoung;Lee, Sangbo;Kim, Jeongho
    • Journal of the Korean Society of Urban Environment
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    • v.18 no.4
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    • pp.485-494
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    • 2018
  • This study aims to identify the characteristics of oxidation and chemical composition of PM in winter season, 2017 at Incheon area. The mean concentration of air pollutants were $46{\pm}22{\mu}g/m^3-PM_{10}$, $29{\pm}18{\mu}g/m^3/-PM_{2.5}$, $5{\pm}3ppb-SO_2$, $0.56{\pm}0.24ppm-CO$, $21{\pm}13ppb-O_3$ and $28{\pm}17ppb-NO_2$, respectively. The dominant ion of the $PM_{1.0}$ chemical component were organic with $3.2{\mu}g/m^3$ and nitrate with $1.9{\mu}g/m^3$. The day and night variation of the $PM_{1.0}$ chemical components was higher in nighttime than those of daytime. The averaged nitrate oxidation rate (SOR) was 0.06 and sulfate oxidation rate was 0.11 during the field campaign. In the high mass loading period, nitrate oxidation rate (NOR) was up to 0.6 and also the nitrate in $PM_{1.0}$ was increased. The averaged ratio of $NO_x/SO_2$ was 8.7 and nitrate/sulfate was 3.1, respectively. In this results, the nitrate component in $PM_{1.0}$ was influenced by NOx from the stationary source as power plant and the mobile source around the measurement site.

Effects of Straight Body Exercise Therapy on Neck Pain, Necks Disability Index and Neck Length Among Turtle Neck Syndrome Patient (정체 운동이 거북목 증후군의 경부통증과 경추 기능장애지수 및 목 길이에 미치는 효과)

  • Kim, Seung Won;Kwak, Jong Hyeok;Kim, Gyeong Rip;Choi, Min Gyeong;Sung, Hyun Chul;Song, Geun Sung
    • Journal of the Korean Society of Radiology
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    • v.15 no.2
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    • pp.201-210
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    • 2021
  • This study was conducted not only to find out the effect of straight body exercise on neck pain, necks disability and neck length change of turtle neck syndrome patients but also to provide fundamental data for this field in the future. Number of 60 persons were selected by pre-test and divided to 21 persons in straight body exercise group, 19 persons in exercise group and 20 persons in control group(non-exercise group). 5 kinds of straight body exercise were applied in straight body exercise group, 8 kinds of strengthening cervical vertebral exercise were applied in exercise group and no exercise was applied in control group. Neck pain, necks disability index and neck length were measured before and after exercise and 10 weeks later as well. The data were analyzed by SPSS(v18.0). In straight body exercise group and exercise group, neck length, neck pain and necks disability index were significantly decreased after eight weeks of exercise and there was no significant difference in control group. Neck length which was checked every week in straight body exercise group and exercise group was significantly decreased and there were significant differences after 3 weeks.. After 2 weeks of 8 weeks exercise, neck length, neck pain and necks disability were checked again in post test and those results were maintained just like 8 weeks exercise status. As above results, it was more efficient in the straight body exercise group than exercise group and control group to decrease neck pain, necks disability and neck length of turtle neck syndrome patients. Furthermore, after 2 weeks of 8 weeks exercise, the results were maintained just like 8 weeks exercise status. Therefore, the author consider that straight body exercise will be effective exercise for turtle neck syndrome patients, and this study would provide fundamental data for this field in the future.

Development of High-frequency Data-based Inflow Water Temperature Prediction Model and Prediction of Changesin Stratification Strength of Daecheong Reservoir Due to Climate Change (고빈도 자료기반 유입 수온 예측모델 개발 및 기후변화에 따른 대청호 성층강도 변화 예측)

  • Han, Jongsu;Kim, Sungjin;Kim, Dongmin;Lee, Sawoo;Hwang, Sangchul;Kim, Jiwon;Chung, Sewoong
    • Journal of Environmental Impact Assessment
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    • v.30 no.5
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    • pp.271-296
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    • 2021
  • Since the thermal stratification in a reservoir inhibits the vertical mixing of the upper and lower layers and causes the formation of a hypoxia layer and the enhancement of nutrients release from the sediment, changes in the stratification structure of the reservoir according to future climate change are very important in terms of water quality and aquatic ecology management. This study was aimed to develop a data-driven inflow water temperature prediction model for Daecheong Reservoir (DR), and to predict future inflow water temperature and the stratification structure of DR considering future climate scenarios of Representative Concentration Pathways (RCP). The random forest (RF)regression model (NSE 0.97, RMSE 1.86℃, MAPE 9.45%) developed to predict the inflow temperature of DR adequately reproduced the statistics and variability of the observed water temperature. Future meteorological data for each RCP scenario predicted by the regional climate model (HadGEM3-RA) was input into RF model to predict the inflow water temperature, and a three-dimensional hydrodynamic model (AEM3D) was used to predict the change in the future (2018~2037, 2038~2057, 2058~2077, 2078~2097) stratification structure of DR due to climate change. As a result, the rates of increase in air temperature and inflow water temperature was 0.14~0.48℃/10year and 0.21~0.41℃/10year,respectively. As a result of seasonal analysis, in all scenarios except spring and winter in the RCP 2.6, the increase in inflow water temperature was statistically significant, and the increase rate was higher as the carbon reduction effort was weaker. The increase rate of the surface water temperature of the reservoir was in the range of 0.04~0.38℃/10year, and the stratification period was gradually increased in all scenarios. In particular, when the RCP 8.5 scenario is applied, the number of stratification days is expected to increase by about 24 days. These results were consistent with the results of previous studies that climate change strengthens the stratification intensity of lakes and reservoirs and prolonged the stratification period, and suggested that prolonged water temperature stratification could cause changes in the aquatic ecosystem, such as spatial expansion of the low-oxygen layer, an increase in sediment nutrient release, and changed in the dominant species of algae in the water body.

Evaluation of Manganese Removal from Acid Mine Drainage by Oxidation and Neutralization Method (산화법과 중화법을 이용한 산성광산배수 내 망간 제거 평가)

  • Kim, Bum-Jun;Ji, Won-Hyun;Ko, Myoung-Soo
    • Economic and Environmental Geology
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    • v.53 no.6
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    • pp.687-694
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    • 2020
  • Two oxidizing agents (KMnO4, H2O2), and one neutralizing agent (NaOH) were applied to evaluate Mn removal in mine drainage. A Mn2+ solution and artificial mine drainage were prepared to identify the Fe2+ influence on Mn2+ removal. The initial concentrations of Mn2+ and Fe2+ were 0.1 mM and 1.0 mM, respectively. The injection amount of oxidizing and neutralizing agents were set to ratios of 0.1, 0.67, 1.0, and 2.0 with respect to the Mn2+ mole concentration. KMnO4 exhibited a higher removal efficiency of Mn2+ than did H2O2 and NaOH, where approximately 90% of Mn2+ was removed by KMnO4. A black MnO2 was precipitated that indicated the oxidation of Mn2+ to Mn4+ after an oxidizing agent was added. In addition, MnO2 (pyrolusite) is a stable precipitate under pH-Eh conditions in the solution. However, relatively low removal ratios (6%) of Mn2+ were observed in the artificial mine drainage that included 1.0 mM of Fe2+. The rapid oxidation tendency of Fe2+ as compared to that of Mn2+ was determined to be the main reason for the low removal ratios of Mn2+. The oxidation of Fe2+ showed a decrease of Fe concentration in solution after injection of the oxidizing and neutralizing agents. In addition, Mn7+ of KMnO4 was reduced to Mn2+ by Fe2+ oxidation. Thus, the concentrations of Mn increased in artificial mine drainage. These results revealed that the oxidation method is more effective than the neutralization method for Mn removal in solution. It should also be mentioned that to achieve the Mn removal in mine drainage, Fe2+ removal must be conducted prior to Mn2+ oxidation.

Organic Matter and Heavy Metals Pollution Assessment of Surface Sediment from a Fish Farming Area in Tongyoung-Geoje Coast of Korea (통영-거제 연안 어류 양식장 표층 퇴적물 중 유기물 및 중금속 오염 평가)

  • Hwang, Dong-Woon;Hwang, Hyunjin;Lee, Garam;Kim, Sunyoung;Park, Sohyun;Yoon, Sang-Pil
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.4
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    • pp.510-520
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    • 2021
  • To understand the status of organic matter and heavy metal pollution in surface sediment of a fish farming area, we have measured the concentrations of total organic carbon (TOC), total nitrogen (TN), and heavy metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, and Zn) in surface sediments of a fish farming area near Tongyoung-Geoje coast. The mean concentrations of TOC and TN were 22.7 mg/g and 3.4 mg/g, respectively, and were much higher than those in surface sediments of a semi-enclosed bay in the southern coast of Korea. The mean concentrations of As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, and Zn were 10.5 mg/kg, 0.37 mg/kg, 82.9 mg/kg, 127 mg/kg, 4.19%, 0.041 mg/kg, 596 mg/kg, 39.5 mg/kg, and 175 mg/kg, respectively, and the mean concentrations of Cd and Cu were three times higher than those in surface sediments of shellfish farming area in the southeastern coast of Korea. In addition, the concentrations of TOC and corrected Cu exceeded the values of sediment quality guidelines applied in Korea, and pollution load index (PLI) and ecological risk index (ERI) showed that the metal concentrations in the sediments of some fish farming area have a strongly negative ecological impact on benthic organisms, although most metal concentrations did not exceed the sediment quality guidelines. Based on overall assessment results, the surface sediments of fish farming areas in the study region are polluted with organic matter and some heavy metals. Thus, a comprehensive management plan is necessary to improve the sedimentary environments, identify primary contamination sources, and reduce the input of pollution load for organic matter and heavy metals in the sediments of fish farming areas.

Clinical and Epidemiological Characteristics of Common Human Coronaviruses in Children: A Single Center Study, 2015-2019

  • Choi, Youn Young;Kim, Ye Kyung;Choi, Eun Hwa
    • Pediatric Infection and Vaccine
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    • v.28 no.2
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    • pp.101-109
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    • 2021
  • Purpose: Common human coronaviruses (HCoVs) are relatively understudied due to the mild nature of HCoV infection. Given the lack of local epidemiology data on common HCoVs, we aimed to describe clinical and epidemiological characteristics of common HCoVs in children. Methods: Respiratory viral test results from 9,589 respiratory samples from Seoul National University Children's Hospital were analyzed from January 2015 to December 2019. Viral detection was done by the multiplex reverse transcription polymerase chain reaction. Demographics and clinical diagnosis were collected for previously healthy children tested positive for HCoVs. Results: Of the 9,589 samples tested, 1 or more respiratory viruses were detected from 5,017 (52.3%) samples and 463 (4.8%) samples were positive for HCoVs (OC43 2.8%, NL63 1.4%, 229E 0.7%). All 3 types co-circulated during winter months (November to February) with some variation by type. HCoV-OC43 was the most prevalent every winter season. HCoV-NL63 showed alternate peaks in late winter (January to March) and early winter (November to February). HCoV-229E had smaller peaks every other winter. Forty-one percent of HCoV-positive samples were co-detected with additional viruses; human rhinovirus 13.2%, respiratory syncytial virus 13.0%, influenza virus 4.3%. Common clinical diagnosis was upper respiratory tract infection (60.0%) followed by pneumonia (14.8%), croup (8.1%), and bronchiolitis (6.7%). Croup accounted for 17.0% of HCoV-NL63-positive children. Conclusions: This study described clinical and epidemiological characteristics of common HCoVs (OC43, NL63, 229E) in children. Continuing surveillance, perhaps by adding HKU1 in the diagnostic panel can further elucidate the spectrum of common HCoV infections in children.

An Outlier Detection Using Autoencoder for Ocean Observation Data (해양 이상 자료 탐지를 위한 오토인코더 활용 기법 최적화 연구)

  • Kim, Hyeon-Jae;Kim, Dong-Hoon;Lim, Chaewook;Shin, Yongtak;Lee, Sang-Chul;Choi, Youngjin;Woo, Seung-Buhm
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.33 no.6
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    • pp.265-274
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    • 2021
  • Outlier detection research in ocean data has traditionally been performed using statistical and distance-based machine learning algorithms. Recently, AI-based methods have received a lot of attention and so-called supervised learning methods that require classification information for data are mainly used. This supervised learning method requires a lot of time and costs because classification information (label) must be manually designated for all data required for learning. In this study, an autoencoder based on unsupervised learning was applied as an outlier detection to overcome this problem. For the experiment, two experiments were designed: one is univariate learning, in which only SST data was used among the observation data of Deokjeok Island and the other is multivariate learning, in which SST, air temperature, wind direction, wind speed, air pressure, and humidity were used. Period of data is 25 years from 1996 to 2020, and a pre-processing considering the characteristics of ocean data was applied to the data. An outlier detection of actual SST data was tried with a learned univariate and multivariate autoencoder. We tried to detect outliers in real SST data using trained univariate and multivariate autoencoders. To compare model performance, various outlier detection methods were applied to synthetic data with artificially inserted errors. As a result of quantitatively evaluating the performance of these methods, the multivariate/univariate accuracy was about 96%/91%, respectively, indicating that the multivariate autoencoder had better outlier detection performance. Outlier detection using an unsupervised learning-based autoencoder is expected to be used in various ways in that it can reduce subjective classification errors and cost and time required for data labeling.

Analysis on Seismic Resistance Capacity of Hollow Concrete Block Reinforced Foundation Ground by Using Shaking Table Test (진동대 시험을 이용한 중공블록 보강 기초의 내진성능분석)

  • Shin, Eun-Chul;Lee, Yeun-Jeung;Yang, Tae Chul
    • Journal of the Korean Geosynthetics Society
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    • v.20 no.4
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    • pp.85-93
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    • 2021
  • The seventy percentage of Korean Peninsular is covered by the mountainous area, and the depth of west sea and south sea is relatively shallow. Therefore, a large scale land reclamation from the sea has been implemented for the construction of industrial complex, residental area, and port and airport facilities. The common problem of reclaimed land is consisted of soft ground, and hence it has low load bearing capacity as well as excessive settlement upon loading on the ground surface. The hollow concrete block has been used to reinforce the loose and soft foundation soil where the medium-high apartment or one-story industrial building is being planned to be built. Recently the earthquakes with the magnitude of 4.0~5.0 have been occurred in the west coastal and southeast coastal areas. Lee (2019) reported the advantages of hollow concrete block reinforced shallow foundation through the static laboratory bearing capacity tests. In this study, the dynamic behavior of hollow concrete block reinforced sandy ground with filling the crushed stone in the hollow space has been investigated by the means of shaking table test with the size of shaking table 1000 mm × 1000 mm. Three types of seismic wave, that is, Ofunato, Hachinohe, Artificial, and two different accelerations (0.154 g, 0.22 g) were applied in the shaking table tests. The horizontal displacement of structure which is situated right above the hollow concrete block reinforced ground was measured by using the LVDT. The relative density of soil ground are varied with 45%, 65%, and 85%, respectively, to investigate the effectiveness of reinforcement by hollow block and measured the magnitude of lateral movement, and compared with the limit value of 0.015h (Building Earthquake Code, 2019). Based on the results of shaking table test for hollow concrete block reinforced sandy ground, honeycell type hollow block gives a large interlocking force due to the filling of crushed stone in the hollow space as well as a great interface friction force by the confining pressure and punching resistance along the inside and outside of hollow concrete block. All these factors are contributed to reduce the great amount of horizontal displacement during the shaking table test. Finally, hollow concrete block reinforced sandy ground for shallow foundation is provided an outstanding reinforced method for medium-high building irrespective of seismic wave and moderate accelerations.

Habitat Quality Analysis and an Evaluation of Gajisan Provincial Park Ecosystem Service Using InVEST Model (InVEST 모델을 이용한 가지산도립공원의 서식지질 분석과 생태계서비스평가)

  • Kwon, Hye-Yeon;Jang, Jung-Eun;Shin, Hae-Seon;Yu, Byeong-Hyeok;Lee, Sang-Cheol;Choi, Song-Hyun
    • Korean Journal of Environment and Ecology
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    • v.36 no.3
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    • pp.318-326
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    • 2022
  • The Convention on Biodiversity (CBD) recommends that 17% of the land be designated as a protected area to counter global environmental problems. Korea also realized a need to designate protected areas according to the international level and explain the significance of designating protected areas. Accordingly, studies on ecosystem services are required. In Korea, the protected areas are designated as national parks, provincial parks, and county parks by hierarchy under the Natural Parks Act. However, as priority was on political and administrative aspects, research on ecosystem service value evaluation and habitat management were concentrated in national parks, and provincial and county parks were relatively neglected. Therefore, more studies on provincial and county parks are necessary. In this study, habitat quality for Gajisan Provincial Park, where there were few studies on habitat management and ecosystem service valuation, was evaluated using the InVEST Habitat Quality model among the InVEST models. The analysis results were compared with 16 mountainous national parks. The results showed that the habitat quality value of Gajisan Provincial Park was 0.83, higher than that of the surrounding areas. The analysis of habitat quality in three districts showed 0,84 for the Tongdosa and Naewonsa districts and 0.83 for the Seoknamsa district. By use district, the nature conservation district, the natural environment district, the cultural heritage district, and the park village district had the highest habitat quality value in that order. Compared with the existing habitat quality analysis results of national parks, Gajisan Provincial Park showed naturalness at the level of Mudeungsan National Park. These results can be used as objective data for establishing policies and management plans to preserve biodiversity and promote ecosystem services in provincial parks.