• Restorative Dentistry and Endodontics
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• v.32 no.3
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• pp.236-247
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• 2007

The purpose of this study was to evaluate the effects of cyanate methacylate on the shear bond strengths to bovine dentin surfaces as a dentin primers. Seven experimental adhesives were made with different mass fraction of Isocyanatoetylme-thacrylate (IEM), 40wt% HEMA (Wako Pure Chemical Industries Osaka, Japan), 0.6% camphoroquinone, 0.4% amine and ethanol as balance dentin bonding agents (0, 2, 4, 6, 8, 10, 12%) were made and applied on the surface of bovine dentin specimens of 7 experimental groups. Shear bond strengths were measured using a universal testing machine (Instro 4466). To identify the ratio and modes of cohesive failures, microscopic examinationn was performed. The ultra-structure of resin tags were observed under scanning electron microscope. The results were as follows ; 1) A higher shear bond strengths (33.62 MPa) in group 8% of Cyanate methacrylate to dentin were found, but there were no statistically significancy between Groups (p > 0.05). 2) The higher ratio of cohesive failures mode in group 2, 6, an 10% could be seen than that in any other groups. 3) A shorter resin tags were observed in all experimental groups. This could be resulted that the preventing from the cyanate methacrylate penetrate into dentin owing to reacting it with dentin collagen. Therefore the resin tags were shorter in lengths. Whether the higher bonding strengths of dentin bonding agents can be affected was not been assured with statistic results. The results indicated that the relation between tensile strengths of the dentin adhesives to bovine dentin and resin tags formed into the dentin could not affected. The main reason of increasing the shear bond strength to bovine dentin in experimental groups could not be assured.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.390-394
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• 2017

Unexpected antibody screening and identification tests are highly important in the prevention of hemolytic transfusion reactions. Therefore, it is highly recommended to perform unexpected antibody screening test in all transfusion candidates. Here, the frequency and distribution of unexpected antibodies identified in Jeju for the past 3 years were evaluated. Between Jan 2014 and Dec 2016, unexpected antibody screening test was performed for 10,360 sera of transfusion candidates in Jeju general hospital using a column agglutination method with the Ortho BioVue system (Ortho-clinical Diagnostics, Raritan, NJ, USA). Eighty-seven (0.84%) of 10,360 cases that underwent unexpected antibiotics screening showed positive results. Among them, unexpected antibodies were identified in 41 cases (0.40%). Unidentified antibodies were detected in 8 cases (19.51%) and autoantibodies were detected in 3 cases (7.32%). The anti-E antibody included in warm antibodies were detected most frequently in 8 cases (19.51%); 6 cases (14.63%) of anti-E + anti-c antibody and 3 cases (7.32%) of $anti-Le^a+anti-Le^b$. $Anti-Le^a$ and $anti-Le^b$ antibodies were detected in 2 cases (4.88%), respectively. The anti-D, $anti-Di^a$, $anti-Fy^b$, $anti-Jk^a$, $anti-Jk^b$, anti-M and anti-P1 were detected in 1 case (2.44%). Complex antibodies were detected in 1 case (2.44%) in anti-C+anti-D and anti-E+anti-c+$anti-Jk^b$, respectively. In this study, we analyzed the frequency and distribution of unexpected antibodies in one general hospital for the past 3 years. However, there has been a general increase in multicultural families and foreign workers in Jeju, and it would be a meaningful study to compare the frequency and distribution of unexpected antibodies.