• Journal of the Korean Applied Science and Technology
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• v.36 no.3
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• pp.821-829
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• 2019

This study was conducted to evaluate the applicability of the extracts from nipa palm, molokhia, and finger root in functional cosmetics as a natural active ingredient. The extracts were obtained through the processes of heating under reflux with ethanol, filtration, concentration, and freeze-drying. UV absorption and blocking effects of the extracts were examined by using the UV-vis spectrophotometer equipped with an integrating sphere. Antioxidant activity and its stability between the extracts were compared using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Also, total polyphenol content in the extracts was determined quantitatively using the Folin-Ciocalteu reagent, with gallic acid as the standard. Antibacterial activity of the extracts was investigated by the disc diffusion test against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative). Finally, collagenase inhibitor assay was performed to examine the anti-wrinkle effect of the extracts. From the results of this study, the extract of nipa palm showed the potential for use in cosmetics as an antioxidant and anti-wrinkle agent, and the extract of finger root as a sunscreen and antibacterial agent.

• Journal of Life Science
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• v.18 no.6
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• pp.776-781
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• 2008

To develop natural food preservatives, methanol and water extracts were prepared from the Suaeda maritima and their antibacterial activities were examined against 12 microorganisms which were food borne pathogens bacteria, food poisoning microorganisms and food-related bacteria. Methanol extracts exhibited antibacterial activities for the 5 Gram positve and 7 Gram negative bacteria by agar diffusion method, The antibacterial activities and cell growth inhibition were investigated on each strain with the different concentrations of Suaeda maritima extracts. Antibacterial activities were shown in root, stem, furit extracts of Suaeda maritima. However stem and fruit extracts showed weak antibacterial activity against the tested microorganisms. Root extracts showed the highest antibacterial activities against microorganisms tested, such as Bacillus cereus,Bacillus subtilis, Vibrio parahaemolyticus, Staphylococcus aureus. The highest antibacterial activity against bacteria test was found in the methanol extract.

• Food Science and Preservation
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• v.11 no.3
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• pp.400-404
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• 2004

This study was conducted to find the antimicrobial activity of garlic extracts by various processing methods(boiled, pan fried, microwave heated, pickled). Ethanol and water extracts from garlic sample were prepared and antimicrobial activities were determined against 10 microoganisms ; food borne pathogens, food poisoning microoganisms, food-related bacteria and yeasts. The ethanol extracts from the fresh and pickled garlic showed antimicrobial activities for test microoganisms, except lactic acid bacteria and yeast. However, the antimicrobial activities were decreased by heat treatment. The minimum inhibitory concentration(MIC) of the fresh garlic extracts was determined to 0.1 mg/mL against an gram positive bacterium and 0.5 mg/mL against an gram negative bacterium. The antimicrobial activities of the ethanol extracts were affected by heating methods and not by pHs.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.245-249
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• 2005

The flower of Magnolia denudata Desrousseaux(Magnoliaceae) has long been used for treatment of nasal disorder in Korea. The physiological activity of the Magnoliae Flos ethanol extract (MFX) was investigated. MFX showed antimicrobial activity. At doses of 100 and 200 mg/kg, MFX showed significant inhibition on both change in paw volume and vascular permeability. MFX (100 mg/kg) significantly inhibited PAR2 agonist-induced myeloperoxidase (MPO) activity in paw tissue. These results indicate that MFX has anti-inflammatory activity in PAR2-mediated paw edema.

• KSBB Journal
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• v.24 no.4
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• pp.347-352
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• 2009

Ethanol extract (1.0 wt%) of Scutellaria baicalensis $G_{EORGE}$ practically satisfied CTFA (The cosmetics, Toiletry, and Fragrance Association) standard in its antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans which were inoculated in skin toner and skin lotion. At concentrations of the extract less than 1.0 wt% the survival rate was the best for S. aureus and the worst for P. aeruginosa. The antimicrobial effect seems to be due to the damage to the bacterial cell wall as evidenced by the images of a Field Emission-Scanning Electron Microscope. The extract also showed a high anti-oxidant effect, and superoxide dismutase (SOD)-like activity reached 80% at 1,000 ppm. These findings suggest that the extract of Scutellaria baicalensis $G_{EORGE}$ is applicable to cosmetics as a natural preservative and an anti-oxidant.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.7
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• pp.1098-1105
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• 2004

In order to develop natural food preservatives, the ethanol and water extracts of the Saururus chinensis Baill were prepared. Antimicrobial activity was examined against 10 kinds of harmful microorganisms. The ethanol and water extracts showed the most active antimicrobial activity against B. subtilis and E. coli. The ethanol extract showed stronger antimicrobial activity than that of the water extract. However, the extracts did not show any antimicrobial activity against lactic acid bacteria and yeast. The minimum inhibitory concentrations (MIC) of ethanol extracts against B. subtilis and E. coli were 5 to 10 mg/mL, respectively. Antimicrobial activity of the ethanol extract was not destroyed at 40-12$0^{\circ}C$ and pH 3∼11. The ethanol extract was fractionated in the order of hexane, diethyl ether, ethyl acetate, and water fractions. The highest antimicrobial activity was found in the diethyl ether fraction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1382-1388
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• 2008

Antimicrobial activities of green tea extracts used for coarse tea were investigated by disc diffusion method using eight different bacteria. Among the green tea extracts, the 70% ethanol extract demonstrated the strongest antimicrobial activities against Vibrio parahemolyticus (V. parahemolyticus) and Staphylococcus aureus (S. aureus) and thus was further fractionated. Among these fractions, the ethyl acetate fraction showed the strongest antimicrobial activities against V. parahemolyticus, S. aureus, Bacillus subtilis (B. subtilis), and Streptococcus mutans (S. mutans). These activities exceeded that of all extracts and fractions tested in this study. Interestingly, although green tea extracts showed significant antimicrobial activity against Micrococcus luteus (M. luteus), once fractionated, the ethyl acetate fraction did not show any antimicrobial activity against M. luteus. MICs of the ethyl acetate fraction were $5\;\;{\mu}L$/disc against B. subtilis and $3\;{\mu}L$/disc against S. aureus, S. mutans and V. parahaemolyticus. 90% inhibition of B. subtilis was observed with 0.05% ethyl acetate fraction but S. mutans needed over 0.1% ethyl acetate fraction to exhibit the same inhibition as B. subtilis. Antimicrobial activities of ethyl acetate fractions were reduced around 10% by thermal treatment at $121^{\circ}C$ for 20 min. All the results suggest that the 70% ethanol extract as well as the ethyl acetate fraction from green tea used for coarse tea could be further developed into a natural antimicrobial agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.2
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• pp.261-269
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• 2012

In this study, 18 kinds of Korean medicinal herb extracts were examined for anti-microbial activities against pathogenic microorganisms. The methanol (MeOH) extracts from Schizandra chinensis, Rhus javanica and Caesalpinia sappan exhibited antimicrobial activities against most pathogenic microorganisms at concentrations of 5 mg/mL, whereas the other 15 extracts exhibited anti-microbial activities at concentrations of 30 mg/mL. The minimum concentration at which Schizandra chinensis extracts inhibited for S. epidermidis and Bor. bronchiseptica was 0.6 mg/mL. The MeOH extracts from Schizandra chinensis, Caesalpinia sappan, Rhus javanica and Seutellaria baicalensis which had higher anti-microbial activities were subsequently fractionated using 5 different solvents, and further screened for anti-microbial activities. The inhibitory effects of ethyl acetate (EtOAc) extracts on microbial growth were greater compared to any other solvent extracts. In order to investigate the inhibitory effect of Korean medicinal herbs with high anti-microbial activities on microbial proliferation, the MeOH extracts at concentrations of 0, 100, 300 and 500 ppm were added to the media. No addition of extracts caused rapid growth of microbes after 12 hours incubation. As the concentration of extracts from Rhus javanica and Caesalpinia sappan increased, the growth-inhibiting effect on gram-positive bacteria including S. aureus, S. epidermidis, and L. monocytogenes was prominent. Rhus javanica extracts exhibited growth-inhibiting activity for gram-negative bacteria including Sal. Pullorum and Sal. Choleraesuis. The low concentration of extracts from Rhus javanica and Caesalpinia sappan exhibited the growth of Bor. bronchiseptica and E. coli serotype $O_8$. However, the higher concentration of extracts from Rhus javanica and Caesalpinia sappan exhibited a strong inhibitory effect on microbial proliferation.

• Journal of Life Science
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• v.30 no.6
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• pp.550-554
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• 2020

The purpose of this study was to screen the testosterone-like activities of eight oriental medicines with an in vitro testosterone compound detection (TCD) system. Distilled water and 80% ethanol, both at 80℃, were used to obtain extracts from Cervus nippon temminck (Nokgaksang), Cynanchum wilfordii (Baeksuo), Lespedeza cuneata (Yagwanmun), Panax ginseng (Hongsam), Smilax china (Toryeong), Taraxacum platycarpum (Mindlre), Tribulus terresteis (Namgase), and Trigonella foenum-graecum (Horopa), and extracts at concentrations of 5, 50, and 500 ㎍/ml were assessed using the TCD system. The testosterone-like activities of the 80% ethanol extracts were seen to increase with concentration and ranged from 0.9 to 5.0 times higher than those of the negative control; Horopa and Yagwanmun exhibited superior testosterone-like activities, followed by Mindlre and Hongsam at the same concentration. Toryeong and Namgase, on the other hand, showed low activity with Nokgaksang and Baeksuo exhibiting very low levels. Compared with the activities of the positive control (5α-DHT), the 80% ethanol extracts of Horopa and Yagwanmun at a concentration of 500 ㎍/ml showed higher testosterone-like activities than 10^-8 M 5α-DHT. The water extracts showed lower activity levels than the ethanol samples, and the change of activity with concentration was also lower. It was confirmed that the 80% ethanol extracts of Horopa, Yagwanmun, and Mindlre etc. enhance the transcription efficiency of testosterone-related genes and may therefore be useful in developing health food or medicinal treatments for the improvement of male menopausal symptoms.

• Journal of the East Asian Society of Dietary Life
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• v.22 no.1
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• pp.109-119
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• 2012

This study analyzed the antibacterial activity of the ethanol extract of Schizandra chinensis Baillon against food pathogenic microorganisms to determine its capabilities as a natural antimicrobial agent. A paper disc diffusion test, minimum inhibitory concentration (MIC) determination, and time-kill assay showed that the ethanol extract strongly inhibits the growth of Listeria monocytogenes, Bacillus cereus, Escherichia coli O157:H7, and Pseudomonas aeruginosa. Release of cytoplasmic ${\beta}$-galactosidase was detected in E. coli, E. coli O157:H7, S. aureus, and P. aeruginosa treated with the ethanol extract. An increase of outer membrane permeability caused by the ethanol extract was also observed. An outward flow of cell constituents was detected in the Gram negative strains treated with the ethanol extract. These results imply that the inner and outer membranes of cells were partially destroyed and cell constituents were released by the treatment of the S. chinensis Baillon ethanol extract. The results of this study indicate that ethanol extract of S. chinensis Baillon evidences a fairly good antibacterial effect.