• Journal of Genetic Medicine
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• v.5 no.1
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• pp.15-20
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• 2008

Purpose : Large exon deletions in the DMD gene are found in about 60% of DMD/BMD patients. Multiplex PCR has been employed to detect the deletion mutation, which frequently generates noise PCR products due to the presence of multiple primers in a single reaction as well as the stringency of PCR conditions. This often leads to a false-negative or false-positive result. To address this problematic issue, we introduced the dual primer oligonucleotide (DPO) system. DPO contains two separate priming regions joined by a polydeoxyinosine linker that results in high PCR specificity even under suboptimal PCR conditions. Methods : We tested 50 healthy male controls, 50 patients with deletion mutation as deletion-positive patient controls, and 20 patients with no deletions as deletion-negative patient controls using DPO-multiplex PCR. Both the presence and extent of deletion were verified by simplex PCR spanning the promoter region (PM) and 18 exons including exons 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, and 60 in all 120 controls. Results : DPO-multiplex PCR showed 100% sensitivity and specificity for the detection a deletion. However, it showed 97.1% sensitivity and 100% specificity for determining the extent of deletions. Conclusion : The DPO-multiplex PCR method is a useful molecular test to detect large deletions of DMD for the diagnosis of patients with DMD/BMD because it is easy to perform, fast, and cost-effective and has excellent sensitivity and specificity.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.4
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• pp.301-308
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• 2009

Purpose: The aim of this study was to evaluate the usefulness of FDG-PET/CT as follow up imaging tool in patients with endometrial cancer after therapy. Material and Methods: One hundred one patients with endometrial cancer who underwent FDG PET/CT after the treatment of this disease were included in this study population (25-79 yr old, Mean age 50.6 yr old) and all these patients also performed various laboratory and imaging studies such as serum tumor marker, CT or MRI. The lesions having increased focal FDG uptake were classified into benign, equivocal, and malignant one according to their pattern and activity. Tumor recurrence was confirmed by histopathological results and other clinical and imaging data. Results: Among the 19 patients with 30 malignant or equivocal hot uptakes, 11 of 14 patients supposed to be malignant finding in PET/CT were proved to be tumor recurrence, while one of 5 patients with equivocal lesions were recurred malignancy, Two false negative cases were turned out to be peritoneal carcinomatosis, Estimated sensitivity, specificity and accuracy of PET/CT for diagnosis of recurrence in endometrial carcinoma after treatment were 86 %, 92 % and 91 %, respectively. Positive and negative predictive values in the same issue were 63% and 98%, respectively. Conclusion: FDG-PET/CT is useful for regular work up of endometrial carcinoma after the treatment because of its high negative predictive value as well as high sensitivity and specificity.

• The Journal of the Acoustical Society of Korea
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• v.21 no.7
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• pp.656-664
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• 2002

We present a statistical analysis of Korean phonological variations using a Grapheme-to-Phoneme (GPT) system. The GTP system used for experiments generates pronunciation variants by applying rules modeling obligatory and optional phonemic changes and allophonic changes. These rules are derived form morphophonological analysis and government standard pronunciation rules. The GTP system is optimized for continuous speech recognition by generating phonetic transcriptions for training and constructing a pronunciation dictionary for recognition. In this paper, we describe Korean phonological variations by analyzing the statistics of phonemic change rule applications for the 60,000 sentences in the Samsung PBS Speech DB. Our results show that the most frequently happening obligatory phonemic variations are in the order of liaison, tensification, aspirationalization, and nasalization of obstruent, and that the most frequently happening optional phonemic variations are in the order of initial consonant h-deletion, insertion of final consonant with the same place of articulation as the next consonants, and deletion of final consonant with the same place of articulation as the next consonant's, These statistics can be used for improving the performance of speech recognition systems.

• The Journal of the Acoustical Society of Korea
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• v.20 no.7
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• pp.37-46
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• 2001

This paper proposes a new noise suppression method using the Wavelet transform analysis. The noise suppressor using the Wavelet transform shows the more effective advantages in a babble noise than one using the short-time Fourier transform. We designed a new channel structure based on spectral subtraction of Wavelet transform coefficients and used the Wavelet mask pattern with more higher time resolution in high frequency. It showed a good adaptation capability for babble noise with a non-stationary property. To evaluate the performance of proposed noise canceller, the informal subjective listening tests (Mos tests) were performed in background noise environments (car noise, street noise, babble noise) of mobile communication. The proposed noise suppression algorithm showed about MOS 0.2 performance improvements than the suppression algorithm of EVRC in informal listening tests. The noise reduction by the proposed method was shown in spectrogram of speech signal.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.01a
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• pp.185-188
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• 2013

음성통화 위주의 통신 시장이 무선 인터넷 중심으로 변함에 따라 스마트디바이스의 생태계가 진화하고 있다. 일반 데스크탑 환경에서 보여지는 웹을 바탕으로 모바일 디바이스에서도 데스크탑 퀄리티의 환경을 사용할 수 있게 되었다. 또한 UI를 비롯하여 사용자 중심의 각종 서비스들이 개발되고 있으며 향후, 2016년까지 전 세계 모바일 데이터 트래픽이 현재의 10배 수준으로 증가할 것으로 전망하고 있다. 본 논문에서는 기존 모바일디바이스를 활용한 주문 시스템의 단점과 한계를 APNs방식을 사용하여 모바일 데이터사용량을 최소화함으로써 모바일 데이터 트래픽의 낭비가 심한 문제점을 보완하였다. 또한 TLS peer-to-peer방식을 사용하여 신뢰성 있는 접속환경을 만듦으로써 보완성을 높였다. 전송에 대한 반응과 보완성을 높여 기존 방식에 비하여 더욱 안정적인 환경을 구축하였다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.203-203
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• 1994

3) 결과 및 고찰 : 급성독성시험 : 대조군 및 G009투여군(최저 312.5mg/kg, 최고 5000mg/kg) 5용량에서 모두 사망예가 관찰되지 않았다 체중변화에 있어서도 대조군과 투여군 사이에 유의성 있는 차이는 없었다. 육안적 소견은 생존동물 모두에 약물에 기인한 내부장기의 이상이 관찰되지 않았다. 유전독성시험 : 마우스 골수세포를 이용한 소핵시험에서 약물 투여에 의한 어떠한 독성의 징후도 관찰되지 않았다. 포유류 배양세포를 이용한 염색체이상 시험에서 모든 농도에서 염색체 이상을 가진 세포의 출현빈도가 3% 이하로서 G009는 CHL세포에 대하여 염색체 이상유발성이 없었다. 살모넬라균을 이용한 복귀돌연변이 시험에서 투여군은 음성대조와 같은 정도 또는 그 이하의 복귀변이 집락수를 나타내었다.

• Proceedings of the Korean Information Science Society Conference
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• 2001.10c
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• pp.670-672
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• 2001

최근 인터넷에서는 메일, 파일 전송 등의 비실시간 응용 뿐만 아니라 음성, 비디오 등의 실시간 응용 서비스를 이용하려는 요구가 증가하고 있다. 이를 위해 광대역 서비스 뿐만 아니라 사용자별 차별화된 QoS의 보장이 요구된다. 네트워크를 통해 전달되는 패킷의 지연 시간과 데이터 처리율, 그리고 손실은 서비스에 따른 요구사항 등이 QoS의 주요 내용인데, 현재의 인터넷은 모든 패킷을 동일하게 전달하는 최선의 노력(Best-Effort)만을 제공하고 있기 때문에 서비스에 따른 패킷의 전달 지연과 지연 변이에 대한 요구사항을 보장해 주지 못하고 있다. 본 연구에서는 분산된 네트워크 환경에서 효율적인 QoS 관리를 위해 이동 에이전트(Mobile Agents)를 이용한 모니터링 시스템을 설계 및 구현하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1227-1236
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• 2001

Genotoxicological safety on 10 kGy-gamma irradiated vegetable juices such as Oenanthstolonifera DC., Daucus carota L., Brassica oleracea var. acephala and Angelica keiskei was determined by the Salmonella typhmurium reversion assay, the SOS Chromotest using in Escherichia cloi PQ37 and chromosome aberration test in cultured Chinese hamster lung (CHL) fibroblast cells. Vegetable juices exposed to 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests.

• Annual Conference of KIPS
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• 2014.11a
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• pp.187-189
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• 2014

패킷 네트워크에서 음성, 영상 트래픽이 IP, UDP, RTP 를 이용하여 전송 될 때 중복 헤더가 사용되어 통신의 비효율을 초래한다. 이를 방지 하기 위해 ROHC(Robust Header Compression)가 적용된다. 이는 인접 패킷 헤더 간의 차이 값이 규칙적으로 증가하는 영역은 그 차이 값만을 전송한다. 차이 값은 WLSB 인코딩 과정을 거쳐 전송이 되는데 인코팅 시 Sliding Window 값이 사용된다. Sliding Window 크기에 따라 헤더 압축률이 변하고 대역폭에도 영향을 미치게 된다. 본 논문에서는 효과적인 Sliding Window 값을 구하여 기존 ROHC 에 적용 하여 네트워크 통신의 효율성을 향상 시키고자 한다.

• Korean Journal of Poultry Science
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• v.40 no.2
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• pp.83-89
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• 2013

iNOS (Inducible nitric oxide synthase) and TLR-4 (Toll-like Receptor-4) play crucial roles in innate immunity of poultry. iNOS has been mapped to chicken chromosome 14 and implicated in a variety of chicken diseases. iNOS possesses potent antimicrobial activity, including the inhibition of microbes replication in vitro. TLR-4 is a pathogen associated molecular-pattern receptor for bacterial product, such as LPS (lipopolysaccharides) found in Gram negative bacteria, that triggers pro-inflammatory cytokine expression after engagement with ligands. In the previous studies, genetic analysis of iNOS and TLR-4 revealed the possible association of mutation in these genes with the intestinal microflora of cecum when infected with Salmonella spp. This study was aimed to augment previous findings, which show the association of iNOS (C14513T) and TLR-4 (G4409T) polymorphisms with economic traits in Korean Native Black (KNB), Rhode Island Red (RIR) and Cornish chickens. Investigation in the effect of SNPs on economic traits (layday, layw, layno, bw150, bw270, layw270) was conducted. iNOS (C14513T) had a significant effect on the average body weight at 270 days of age (p<0.05) in both KNB and RIR, whereas TLR-4 (G4409T) showed no significant correlation with all traits (p>0.05). The results obtained from using the candidate genes can be useful for the genetic improvement of body weight in both KNB and RIR breeds.