• Title/Summary/Keyword: 유충 발육

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Establishment of Optimal Rearing Conditions for the Production of Tenebrio molitor Larvae (갈색거저리 유충 생산을 위한 효율적인 사육조건 확립)

  • Kim, Sihyeon;Kim, Jong Cheol;Lee, Se Jin;Kim, Jae Su
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.421-429
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    • 2016
  • Tenebrio molitor larvae contain large amounts of proteins, lipids and other functional materials, enabling this insect to be used as an edible food source in animal feeds and for industrialization. Although many efforts have been made to set up mass rearing systems, few studies have been conducted to establish optimal rearing conditions for the production of high quality T. molitor larvae. Herein we investigated 1) the effects of additional diets on the survival and fecundity of the insect, 2) the relationship between oviposition period and the uniformity of larval size, 3) the effects of rearing density and temperature on insect development, and 4) the storage stability of eggs and pupae at low temperatures given possible temporary production discontinuation. The addition of carrot and zucchini to the traditional wheat bran diet significantly increased the survival and fecundity rate of adult T. molitor. Of the three different oviposition sampling periods (3, 7, and 14 days) used to investigate the uniformity of the hatched larvae in each treatment, the period of 3 and 7 days provided higher uniformity than the 14 days oviposition period. Larval development was faster at $30^{\circ}C$ than at 20, 25, and $35^{\circ}C$. Interestingly, oviposition rates were highest at $20^{\circ}C$ but showed much slower larval development and lower uniformity at $30^{\circ}C$. Regarding the effect of larval rearing densities (1, 10, 20, 30, 40, and 50 larvae per 90 mm diam. dish), larval weight was significantly reduced at higher rearing densities, but larval longevity and length were not influenced by rearing density. The 30 larvae/dish is suggested to be a reasonable density to be applied to mass production systems. When kept at $4^{\circ}C$, T. molitor eggs showed a significant reduction in hatching rate; however, when stored under the same conditions, pupae emergence rates remained high until 10 weeks, suggesting that storage at low temperatures is more suitable for the pupal stage than the egg stage. Our findings suggest that an increase in T. molitor adult survival and fecundity rates and a uniformity of hatched larval development can be achieved with the following recommendations: a combination diet (including wheat bran), a 7-day oviposition period; a larvae-rearing temperature of $30^{\circ}C$, a rearing density of 30 larvae/dish, and the storage of pupal stages at low temperatures in the case of rearing discontinuation. This study serves as a strong foundation for the successful mass production of high quality T. molitor larvae.

A Note on the Deveolopment of Tribolium castaneum (Coleoptera: Tenebrionidae) on Brown Rice Oryzae sativa L. (현미에서의 거짓쌀도둑, Tribilium castaneum(딱정발레목:거저리과)의 발육)

  • 전환구;홍영석;류문일
    • Korean journal of applied entomology
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    • v.30 no.2
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    • pp.103-137
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    • 1991
  • The developmental period(mean $\pm$ SE) of the red flour beetle, Tribolium castaneum was studied on brown rice at four constant temperature(25, 28, 34, $36^{\circ}C$ $\pm$ $0.5^{\circ}C$, RH 70%)conditions. Based on the results, the lower and the upper developmental threshold temperature for the beetle were also estimated. The duration of the egg stage was estimated to be 7.6 $\pm$ 0.25, 4.8 $\pm$ 0.10, 3.0 $\pm$ 0.03 and 2.5 $\pm$ 0.09 days at 25, 28, 34, $36^{\circ}C$, respectively. That of the larval stage was estimated to be 53.3 $\pm$ 1.49, 33.4 $\pm$ 0.57, 30.6 $\pm$ 0.70, 31.0 $\pm$ 1.18; that of the pupal stage to be 12.1 $\pm$ 0.17, 7.8 $\pm$ 0.09, 5.7 $\pm$ 0.09, 5.0 $\pm$ 0.07 ; that of the overall period which spans from eggs to the adult emergence to be 72.3 $\pm$ 1.67, 46.0 $\pm$ 0.55, 39.4 $\pm$ 0.64, 38.7 $\pm$ 1.15 days at 25, 28, 34, $36^{\circ}C$, respectively. The duration of the egg and the pupal stage were similar to those of the results reared on other diets such as wheat and maize. Fed on brown rice, however, the duration of the larval stage was significantly retarded than on wheat. The mortality of the beetle during ,development was higher on brown rice than on wheat reported, indicating that brown rice is a poor diet compared with wheat. The lower developmental threshold temperature was estimated to be $20.0^{\circ}C$ for all stages of the beetle. The upper one was estimated to be $40.2^{\circ}C$ for the overall stage. The modal value of the moulting times during the larval stage was 7. However, as temperature increased,.the frequency of the beetle that moulted more than 7 times became higher. The sex ratio of the beetle was not affected by temperature.

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The Effect of Temperature on the Development of Spodoptera frugiperda (Lepidoptera: Noctuidae) (열대거세미나방 발육에 미치는 온도의 영향)

  • Moon, Hyung Cheol;Choi, Min Kyung;Jang, Su Ji;Lee, Jang Ho;Kim, Ju Hee;Chon, Hyong Gwon
    • Korean journal of applied entomology
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    • v.61 no.2
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    • pp.349-356
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    • 2022
  • Fall armyworm, Spodoptera frugiperda, is a invasive migratory pest of native to the tropical and sub-tropical regions that has recently invaded Korea with damage to cornfield. The study was conducted to investigate the development periods of S. frugiperda on artificial diet at six different temperatures. The developmental period from eggs to adult at 18, 21, 24, 27, 30 and 32℃ was 79.8, 54.2, 34.3, 28.4, 24.6 and 24.0 days, respectivery and decreased with increasing temperature. The pupal periods of females were shorter than males. Most of the larvae developed through six instar, but the ratio of 7 or more instar increased at low temperatures. The relationship between the development rate and temperature was fitted with by linear regression analysis. The lower development threshold for egg and larva development was 12.9℃ and 11.3℃, respectively, 12.6℃ for pupae and 11.8℃ for egg-to-adult development. The effective accumulative temperature for the development of the respective life cycle stages were 37.0 DD for eggs, 286.3 DD for larvae, 132.2 DD for pupae and 456.8 DD egg-to-adult development.

Effects of Temperature on the Development of Gypsy moth (Lymantria dispar) (매미나방(Lymantria dispar) 발육에 미치는 온도의 영향)

  • A-Hae Cho;Hyo-Jeong Kim;Jin-Hee Lee;Ji-in Kim
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.385-388
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    • 2023
  • Gypsy moth (Lymantria dispar), a polyphagous insect pest belonging to the family Lymantriidae, is widely distributed in Korea, Japan, Siberia, Europe, and North America. They pose a threat to various host plants including pear trees, apple trees, and blueberries. Traditionally considered a forest pest, the increasing incursion of gypsy moths into agricultural land near forested areas has intensified damage to crops lacking effective control methods. This study aimed to investigate the temperature-dependent development of gypsy moths to enhance outbreak prediction and advance technology development. The effects of temperature on development of each life stage were investigated under constant temperature conditions of 18, 21, 24, 27, 30, and 33℃ (14L:10D, RH 60±5%) utilizing egg masses collected in Jeollanam-do Jangheung-gun in 2021. The results revealed that higher temperatures accelerated the development rate of the gypsy moth larvae with optimal development occurring at 30℃. However, the survival rate was lowest at 33℃. At the favorable temperature of 30℃, the total development period was 43.8 days for females and 42.5 days for males. The developmental threshold temperature were 13.1℃ for females and 12.5℃ for males, with effective accumulated temperature of 641.1 DD and 657.8 DD, respectively.

Rearing Method for Ascotis selenaria (Lepidoptera: Geometridae) using an Artificial Diet (인공사료를 이용한 네눈쑥가지나방(Ascotis selenaria)(나비목: 자나방과) 실내 사육법)

  • Choi, Kyung-San;Park, Young-Mi;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.50 no.1
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    • pp.55-63
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    • 2011
  • This study was conducted to develop an artificial diet for the mugwort looper, Ascotis selenaria (Lepidoptera: Geometridae), which is an insect pest to leaves of citrus (Citrus unshiu). Corn and soybean powder were selected as main nutrient sources for larvae of A. selenaria after several diets consisted of wheat germ, corn, kidney bean and/or soybean were tested for larval development and survival. A higher amount of the main nutrients in the diet increased the larval survivorship. Addition of yeast and cholesterol in diet increased the larval survivorship. Finally the composition of diet was decided as followings; corn 100 g, soybean 100 g agar 25 g, Brewers' yeast 30 g, cholesterol 0.5 g, Vanderzant vitamin mixture 2 g, Wesson's salt mixture 2 g, sorbic acid 2 g, ascorbic acid 2 g, and methyl-4-hydroxybenzoate 2.5 g, and distilled water 1 liter. Development periods of larvae and pupae, survival rate and fecundity of A. selenaria reared on the diet were not significantly different with those on the host plant, citrus leaves. Larvae of early instars were reared in a group, while larvae of later instars (5-6th) were reared individually. Adult mating was conducted in a plastic cage and an oilpaper covered with a gauze was provided as an oviposition site.

Development and Adult Life Span of Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae) Fed on the Melon Aphid, Aphis gossypii Glover or the Green Peach Aphid, Myzus persicae($S\"{u}lzer$) (Homoptera: Aphididae) (목화진딧물(Aphis gossypii)과 복숭아혹진딧물(Myzus persicae) (Homoptera: Aphididae)을 먹이로 한 진디혹파리[Aphidoletes aphidimyza (Rondani)] (Diptera: Cecidomyiidae)의 발육 및 성충수명)

  • Kim Tae-Heung;Kim Ji-Soo
    • Korean journal of applied entomology
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    • v.43 no.4 s.137
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    • pp.297-304
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    • 2004
  • The development of Aphidoletes aphidimyza, an aphidophagous gall midge, was studied at various constant temperatures ranging from 15 to $35^{\circ}C$, with $65{\pm}5\%$ RH, and a photo-period of 16L:8D. When A. aphidimyra was fed either on Aphis gossypii or Myzus persicae, it took 43.9 and 44.5 days, respectively, to develop from egg to pupa at $15^{\circ}C$, whereas at $25^{\circ}C$, 14.3 and 15.8 days. The developmental zero was 10.7 and $10.0^{\circ}C$, respectively, while the effective accumuative temperatures were 210.8 and 245.5 day-degrees. The nonlinear shape of temperature-dependent development, shown by A. aphidimyza when fed on either species of the aphids, was well described by the modified Sharpe and DeMichele model. When distribution model of completion time of development for each growth stage was expressed as physiological age and fitted to the Weibull fuction, the completion time of development gradually shortened from egg to larva, and to pupa. In addition, the coefficient of determination $r^2$ ranged between 0.86-0.93 and 0.85-0.94, respectively providing a good approximation of cumulative developmental rates. The life span of adult was 8.7 and 9.2 days at $15^{\circ}C$, and 3.1 and 2.7 days at $30^{\circ}C$, respectively. Egg incubation period was relatively short at $35^{\circ}C$ but hatchability was less than $50\%$ and the mortality of the larva at $35^{\circ}C$ reached $100\%$. At $30^{\circ}C$, the time of development lengthened and the adult longevity was short suggesting ill effect of high temperatures. Even though the life span of adults at $15^{\circ}C$ was relatively long, none moved freely in the rearing cage and no oviposition occurred. Accordingly, in case A. aphidimyza is adopted to suppress phytophagus aphid populations, it could be applicable to cropping systems with ambient temperatures above $20^{\circ}C$ and below $30^{\circ}C$. Within this range, A. aphidimyza adults was observed to be active and oviposit fully.

Effect of Temperature on Development and Reproduction of the Persimmon Fruit Moth, Stathmopoda masinissa (Lepidoptera: Stathmopodidae) (감꼭지나방(나비목: 감꼭지나방과)의 발육과 생식에 미치는 온도의 영향)

  • 박은철;최경환;김정화;조수원;김길하
    • Korean journal of applied entomology
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    • v.40 no.4
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    • pp.297-300
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    • 2001
  • Development and reproduction of the persimmon fruit moth, Stathmopoda masinissa, were investigated under different temperatures (15, 20, 25, and 3$0^{\circ}C$). It took 96.1 days to grow from egg to adult at 2$0^{\circ}C$, 43.2 days at $25^{\circ}C$, and 34.6 days at 3$0^{\circ}C$. At 15$^{\circ}C$, all tested individuals died before pupation. The developmental threshold temperatures for egg, larva, pupa, and adult were 12.2, 13.5, 13.8, and 13.4$^{\circ}C$, respectively. The total effective temperatures for egg, larva, pupa, and egg to adult were 74.0, 331.3, 160.5, and 569.9 degree days, respectively. The hatching, pupation, and emergence rates were highest at $25^{\circ}C$. The average life span of adult prior to laying eggs and the total life span of adult were 12.6 and 29.3 days at 2$0^{\circ}C$, 3.8 and 8.6 days at $25^{\circ}C$, and 2.5 and 7.0 days at 3$0^{\circ}C$, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (R$_{o}$) was lowest at 2$0^{\circ}C$. The intrinsic rate of natural increase (r$_{m}$) was highest at $25^{\circ}C$ as 0.066.

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Effects of Temperature on Survival, Development, and Reproduction of the Non-diapause Asian Corn Borer, Ostrinia furnacalis (Lepidoptera: Crambidae) (조명나방(나비목: 포충나방과) 비휴면태의 생존과 발육, 생식에 미치는 온도의 영향)

  • Jung, Jin Kyo;Seo, Bo Yoon;Kim, Eun Young
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.449-462
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    • 2021
  • We analyzed the effects of temperature on the survival, development, and reproduction of the non-diapause Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). A 16:8 h light:dark photoperiod was supplied to the non-diapause stages of O. furnacalis. The insects were raised on an artificial diet at seven constant temperatures between 15 and 35℃ for immature stages, and eight temperatures between 13 and 33℃ for the adult stage. The survival rate of eggs exceeded 70% at all temperatures, whereas survival of larvae was as low as 7.4% at 15℃. The developmental periods of the immature stages decreased with increasing temperature, but the larval period did not decrease further at 35℃. The pupal weight increased with increasing temperature, but the female weight decreased at 35℃. Variations with different last larval instars within the colonies at different temperatures were observed. Adults produced offspring at all tested temperatures. Adult longevity and the pre-oviposition and oviposition periods decreased with increasing temperature, but the pre-oviposition period increased at 33℃. Total fecundity exceeded 400 viable eggs at 22℃ and 31℃. The mean daily fecundity during the oviposition period and the mean daily fecundity on the day of oviposition increased with increasing temperature, but decreased at 33℃. Daily fecundity sharply increased at earlier adult ages and slowly decreased thereafter. The simulated oviposition frequency was greatest at 22℃. The lower developmental threshold temperatures were estimated to be the lowest at 9.7℃ for the first instar larva and the highest at 14.7℃ for the 5th to last instar larval stage, using a linear model.

Cold hardiness of Spodoptera litura Fabricius (Lepidoptera: Noctuidae) (담배거세미나방(Spodoptera litura Fabricius)의 내한성)

  • 김용균;박형근;송원례
    • Korean journal of applied entomology
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    • v.36 no.3
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    • pp.256-263
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    • 1997
  • Supercooling points (SCP) and cold tolerance of the tobacco cutworm, Spodol~rera litura Fabricius. were measured in response to subzero temperatures. SCPs were varied among developmental stages. Eggs showed the lowest SCP (-27$^{\circ}$C). Pupae and adults had the intermediate SCP(- 18$^{\circ}$C). The SCPs (- I0 to - 16$^{\circ}$C) of larvae increased with their ages. Lethal low temperature of each stage was higher than its SCP. Preexposure of eggs and larvae to a sublethal low temperature increased their survival capacities under lethal low temperatures. The sublethal temperature also induced the fifth instar larvae to increase hemolymph osmolality and to produce cold-induced proteins of apparent molecular weights of 20 and 27 kD. These results indicate that this species is classified into a freeze-susceptible insect.

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An Artificial Diet for Rearing of Chilo suppressalis (Lepidoptera: Crambidae) Larvae (이화명나방(나비목: 포충나방과) 유충 사육을 위한 인공사료)

  • Jung, Jin Kyo;Kim, I Hyeon;Kim, Eun Young;Seo, Bo Yoon;Moon, Youn-Ho
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.473-477
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    • 2021
  • An artificial diet containing wheat germ, soybean, corn, yeast, sucrose, casein, cholesterol, 𝛽-sitosterol, and dry Miscanthus sacchariflorus stem powder was developed for rearing Chilo suppressalis (Crambidae) larvae. Neonate larvae were individually raised on the diet till adult emergence at 25℃ and 15:9 h light:dark photoperiod. In the results, the pupation and the emergence rates were 82.2% and 98.6%, respectively. The mean developmental periods of female and male larvae were 34.6 days and 31.0 days, respectively, whereas the pupal periods of female and male were 8.9 days and 9.7 days, respectively. Those periods were significantly different between genders. The mean pupal weights of female and male were 75.0 mg and 57.0 mg, respectively, with significant difference.