• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.31-42
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• 2008

It was revaluated about taxonomic position of Bupleurum latissimum Nakai (Apiaceae) by the cluster analysis, the principal component analysis, and the discriminant analysis. Its diagnostic characteristics include bracteole length longer than umbellules, 16-21 pedicel numbers, plant with evergreen leaves, chromosome number 2n = 16 and cauline leaves with auriculate base. It is morphologically similar to B. euphorbioides in having ovate bracteoles, 16-21 pedicel numbers, chromosome number 2n = 16, and cauline leaves with auriculate base, but distinguished from B. longiradiatum with having linear bracteoles, 7-15 pedicel numbers, and chromosome number 2n = 12. Morphological characters support that Bupleurum latissimum Nakai is a Korean endemic species.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.303-318
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• 1995

Microscopic structures of epidermis and palynology and isozyme analysis were examined to find out the intergeneric and interspecific relationships and consequently to confirm the position of Hanabusaya aiatica as an endemic genus among 4 genera and 9 species belonging to the Hanabusaya asiatica, Korean endemic, and its allied groups. In the examination of microscopic structures of epidermis, cell patterns of perianth and ultrastructure of seed coat were found to be useful characters for the identification of the 4 genera and 9 species. Palynological characters such as microscopic structure and overall morphology of pollen grains were, however, not enough to distinguish them because of the great variabilities in these traits. Isozyme analysis showed that H. asiatica was very closly related to Campanula punctata and C. takesimana, though there were variations among populations and collected areas in some classified groups, depending on classified groups. Based on these results, the position of H. asiatica as an endemic genus was well confirmed.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.274-285
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• 2007

The subfamily Chrominae of damselfishes (Teleostei: Pomacentridae) includes the genus Chromis and Dascyllus. They are found throughout the tropical oceans and form a major component of coral reef communities. There are 5 species of the Chrominae currently recognized in Korea. This study was conducted to infer phylogenetic position of two Korean Chromis species and one Dascyllus species within general category of their each genus in worldwide level. This study also includes one species of Japanese Dascyllus. In the phylogenetic analysis, the Japanese D. aruanus grouped with D. aruanus previously reported from French Polynesia. Korean Chromis fumea grouped with Australian C. nitida and the p-distance value between the two species is relatively very low (0.047). Korean C. notatus grouped together with C. flavomaculata (New Caledonia). In the sequence analysis of some Korean and Japanese damselfishes, there was no sequence variation between D. melanurus (Jeju, Korea) and D. melanurus (Indo-Pacific), but the sequences of the two populations were different in only one nucleotide sites from that of D. melanurus in Indonesian Archipelago. The sequences of Dascyllus aruanus (Japan) were different in two nucleotide sites from it in French Polynesia. There were high difference between the sequences of two Korean species, Chromis fumea and Korean C. notatus. The variations among mitochondrial cytochrome b sequences indicate that the gene sequence could be used as DNA barcode for identification of local populations of D. aruaus and D. melanurus as well as species level.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.353-360
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• 2011

The sequence data of the plastid DNA (trnL-F IGS, trnL intron, and trnH-psbA) and nuclear DNA (RNApol2_i23) markers were utilized to study phylogenetic relationships among the taxa in the Polygonatum odoratum complex (Ruscaceae). European P. odoratum individuals form a clade with a high bootstrap value, which is a sister to the clade of Korean P. odoratum var. odoratum, P. odoratum var. pluriflorum and P. robustum. The formation of the clade with P. odoratum var. odoratum, P. robustum, and one accession of P. odoratum var. pluriflorum indicates geological speciation in isolated populations in the islands following dispersal events from the mainlands. All data sets form two major clades, which are congruent with the subgroups divided by the basic chromosome numbers (x = 9 and x = 10). Although it is not easy to test the hypothesis of the decrease in the basic chromosome number due to scatter taxon sampling in this study, the molecular data strongly suggested that aneuploidy plays an important role in lineage diversification in the genus Polygonatum. The cytological data was not strongly supported by the cpDNA sequences. Further investigations of the cytological, morphological, and geographical characteristics with comprehensive sampling are desired to understand the evolution and lineage diversification in the genus.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.312-323
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• 1999

External morphology, principal component analysis, cluster analysis, and vegetation were investigated to understand the taxonomic relationships and native environmental characters of Megaleranthis saniculifolia populations in four different habitats. Morphological characters such as calyx length and width, calyx index, presence or absence of serrate in calyx lobe, length of peduncle, branch of peduncle and fruit characters were useful for the identification of four different habitats. But, characters of plant height, bract and seed in four habitats were similar. The results obtained based on the principal component(PC) analysis of treated 96 OTU were divided into two groups by PC 1, 2, 3, and the sums of contributions for the total variance were 66.79%(PC1 31.3%, PC2 20.7%, PC3 15.8%, respectively), and only Mt. Taeki population was distinctly different from populations of other three habitats. In cluster analysis based on average linkage cluster analysis and Ward's method, there were similarities in the composition of clustered taxa, and each populations were not identified. Importance value by relative coverage and frequency appeared in M. saniculifolia(50.81%), Aruncus dioicus(12.64%), Corydalus turtschanovii(11.62%), Veratrum oxysepalum(11.45%), Anemone koraiensis(8.96%), Meehania urticifolia(8.76%), Filipendula palmata(7.06%), Aconitum pseudo-laeve(5.66%), Pseudostellaria palibiniana(5.45%) and Smilacina japonica(5.25%), respectively. These species were considered to be highly similar with M. saniculifolia. The highest importance value in all investigated sites was M. saniculifolia, but specific composition of high level different from each habitat. Average diversity of species was 1.40, and the highest in Mt. Kwangdeok(1.31), lowest in Mt. Jumbong(1.17). Average soil pH was 5.25 and similar in each habitat. Although the lowest content of K ion, but the highest the EC, water capacity, organic compound, Ca content were found in soil of Mt. Sobaek. Soils in Mt. Kwangdeok had the lowest content of EC, organic compound, Ca. Soils of Mt. Jumbong showed the highest of Mg content, but the lowest of water capacity, $P_2O_5$, and K contents.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.189-195
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• 2006

We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon's Index $0.358{\sim}0.467$(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(${\phi}_{ST}$) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

• Korean Journal of Plant Taxonomy
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• v.42 no.2
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• pp.161-166
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• 2012

Aiming to determine the taxonomic identity of Gang-hwa-yak-ssuk as a cultivated plant, this study analyzes ITS sequences and compares their external morphologies with the genus Artemisia, which have a similar external morphology. Thus far, we have considered A. indica as the original plant of Gang-hwa-yak-ssuk, but Gang-hwa-yak-ssuk is better grouped with A. argyi, not A. indica, for the following reasons: Gang-hwa-yak-ssuk has the same characteristics as A. argyi in terms of the natural habitat, in that it is 1-bipinnately cleft or parted in the external morphology of its leaves, and because the white glandular trichome is distributed on the adaxial surface. This is in addition to the result of ITS sequence analysis. Therefore, we can define Gang-hwa-yak-ssuk as a cultivated plant that originates from A. argyi and not A. indica.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.209-214
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• 2003

In order to clarify the successional trend and its characteristics of plant community in abandoned field in Cheju Island, we investigated the seral changes of vegetation height, coverage, growth form, species richness, diversity and dominance index along with the time lapse, and then compared with those of mainland researches. And in order to seek the overall trend of vegetation changes, we used cluster, TWINSPAN and PCA ordination techniques. The succession was characterized by the lower species number, lower vegetation height, longer period of herb dominant and later invasion of tree species. These results may come from that ecological traits of Cheju Island itself, and ecosystem fragmented influences that block a seed (propagule) transport. Sere of the dominant species was shown as follows: Mazus japonicus(0.5∼1 years)→Conyza sumatrensis (2 years)→Artemisia princeps var. orientalis(4 years)→Artemisia princeps var. orientalis, indigofera pseudotinctoria(5 years)→Rosa multiflora, Miscanthus sinensis, etc.(8 years)→Miscanthus sinensis(12 years)→Boehmeria nivea, Pueraria thunbergiana etc.(15 years)→Mallotus japonicus(20 years)→Litsea japonica, Machilus thunbergii (20 years<). Abandoned fields were classified into three groups according to time lapse; earlier stage(0∼1 years), middle stage(2∼8 years) and later stage(8∼20 years).

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.345-354
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• 2013

To understand the community structures of the vegetation of the beaches and coastal dunes of Seocheon-gun, Boryeong-si, Anmyeon-do, Taean-gun in Chungcheongnam-do and Buan-gun in Jeollabuk-do at the west coast of South Korea, a plant sociological vegetation survey was carried out to make a classification of correlation vegetation communities, a cluster analysis and an inorganic component analysis on the soil and plant body. Regarding the vegetation of the coastal dunes, plant communities were classified into 31 communities by correlation dominant species, and as a result of a cluster analysis on the vegetation communities, they were classified into five vegetation types such as Calystegia soldanella community, Rosa rugosa community, Imperata cylindrica community, Vitex tend community and Elymus mollis community. The soil pH was highest in Seocheon area ($7.54{\pm}1.18$) while lowest in Buan area ($6.29{\pm}2.15$), and regarding inorganic components, the nitrogen content was highest in Robinia while lowest in Imperata cylindrica. The soil PH of vegetation I was highest (8.23) among the five vegetation types, and the organic matter was lowest (71.58). As a result of CCA ordination analysis to examine the correlation among environmental factors, Calystegia soldanella community, Rosa rugosa community and Imperata cylindrica community among the five vegetation types were distributed while they were not affected by soil environmental elements, but Vitex tend community and Elymus mollis community were distributed mainly at the places with high $K^+$ content in the soil.

• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.63-68
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• 2013

ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.